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Image Search Results
Journal: Psychiatry investigation
Article Title: Memory Decline and Aberration of Synaptic Proteins in X-Linked Moesin Knockout Male Mice.
doi: 10.30773/pi.2024.0186
Figure Lengend Snippet: Figure 5. Moesin-KO neurons decreased cell viability and the ex pression of neuronal cell markers. Relative expression levels of GFAP, MAP2, Sox2, DCX, SNAP25, PSD95, and moesin are dis played. At 7 days (A) and 14 days (B) after seeding cortical neu rons, molecular changes in neuronal cell markers were examined by immunoblotting. All data were analyzed by t-test and are pre sented as the mean±SEM of three replicates. The bar is 100 µm. *p< 0.05; **p<0.01; ***p<0.001. KO, knockout mice; WT, wild-type mice.
Article Snippet: The tissue lysates (30 μg) were electrophoresed and transferred to 0.45 μm nitrocellulose membranes, which were incubated with anti-GFAP (ab7260, 1:1,000, Abcam, Cambridge, MA, USA), anti-MAP2 (ab5392, 1:1,000, Abcam), anti-Sox2 (#4900, CST, 1:1,000), anti-DCX (#91954, CST, 1:1,000),
Techniques: Expressing, Western Blot, Knock-Out