Journal: eLife

Article Title: Amoxicillin-resistant Streptococcus pneumoniae can be resensitized by targeting the mevalonate pathway as indicated by sCRilecs-seq

doi: 10.7554/eLife.75607

Figure Lengend Snippet:

Article Snippet: Chemical compound and drug , RADA , Tocris Bioscience , 6649 , Work concentration 250 μM RRID: SCR_014237.

Techniques: Sequencing, In Vivo, Injection, Concentration Assay, Software, Imaging

Journal: The Journal of Biological Chemistry

Article Title: Sidt2 inhibits islet β-cell dedifferentiation by regulating insulin secretion

doi: 10.1016/j.jbc.2025.110544

Figure Lengend Snippet: Disrupted insulin secretion induced β-cell dedifferentiation after Sidt2 ablation. ( A ) Protein expression of FoxO1 and p-FoxO1 in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( B , E and F ) Statistical analysis of data from pane l ( A ). ( C ) Protein expression of FoxO1 after separation of nuclear and cytoplasmic fractions in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( D ) Statistical analysis of data from panel ( C ). ( G ) Immunofluorescence staining of FoxO1 in Sidt2 +/+ and Sidt2 −/− INS-1 cells. Scale bar = 10 μm. ( H ) Protein expression of IRE1α, HSP90B1, Grp78/Bip, and CHOP in Sidt2 +/+ and Sidt2 −/− INS-1 cells. ( I ) Statistical analysis of data from panel ( H ). ( J ) Insulin content in the supernatant of INS-1 cells stimulated with octreotide (Oc) at different concentrations. ( K ) Protein expression of β-cell markers in INS-1 cells stimulated with Oc at different concentrations for 24 h. ( L ) Statistical analysis of data from panel ( K ). ( M ) Protein expression of β-cell markers in INS-1 cells stimulated with Oc at 0 to 50 mg/L. ( N ) Statistical analysis of data from panel ( M ). ( O ) mRNA levels of FoxO1, MafA, and Pdx1 in INS-1 cells stimulated with Oc at 0 to 50 mg/L. ( P ) Insulin content in the supernatant of INS-1 cells stimulated with 50 mg/L Oc at different times. ( Q ) Protein expression of β-cell markers in INS-1 cells stimulated with 50 mg/L Oc at different times. ( R ) Statistical analysis of data from panel ( Q ). ( S ) Protein expression of β-cell markers in in Sidt2 +/+ and Sidt2 −/− INS-1 cells stimulated with 50 mg/L Oc for 24 h. ( T ) Statistical analysis of data from panel ( S ). ( U ) mRNA levels of MafA and Pdx1 in Sidt2 +/+ and Sidt2 −/− INS-1 cells stimulated with 50 mg/L Oc for 24 h. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ## p < 0.01. n = 3.

Article Snippet: INS-1 cells were cultured in RPMI 1640 medium with 0 to 100 mg/L octreotide acetate (Hy-17365, MedChemExpress) for 0 to 48 h. The supernatant was collected, and the insulin content was measured using an insulin enzyme-linked immunosorbent assay (ELISA) kit (E-EL-R2466C, Elabscience Biotechnology Inc) to determine the optimal concentration and incubation time for inhibiting insulin secretion by Oc.

Techniques: Expressing, Immunofluorescence, Staining

Journal: The Journal of Biological Chemistry

Article Title: Sidt2 inhibits islet β-cell dedifferentiation by regulating insulin secretion

doi: 10.1016/j.jbc.2025.110544

Figure Lengend Snippet: Promoting insulin secretion partially mitigated β-cell dedifferentiation disorder induced by Sidt2 KO. ( A ) Insulin content in the supernatant of INS-1 cells stimulated with different concentrations of glimepiride (GM). ( B ) Protein expression of β-cell markers in INS-1 cells stimulated with different concentrations of GM. ( C ) Protein expression of β-cell markers in Sidt2 +/+ and Sidt2 −/− INS-1 cells stimulated with 100 μM GM ( D ) mRNA levels of MafA and Pdx1 in Sidt2 +/+ and Sidt2 −/− INS-1 cells stimulated with 100 μM GM. ( E ) Immunofluorescence staining of INS, Pdx1, and FoxO1 in Sidt2 +/+ and Sidt2 −/− INS-1 cells stimulated with 50 mg/L octreotide. Scale bar = 10 μm. ( F ) Immunofluorescence staining of INS, Pdx1, and FoxO1 in Sidt2 +/+ and Sidt2 −/− INS-1 cells stimulated with 100 μM GM. Scale bar = 10 μm. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; # p < 0.05, ## p < 0.01, ### p < 0.001. n = 3.

Article Snippet: INS-1 cells were cultured in RPMI 1640 medium with 0 to 100 mg/L octreotide acetate (Hy-17365, MedChemExpress) for 0 to 48 h. The supernatant was collected, and the insulin content was measured using an insulin enzyme-linked immunosorbent assay (ELISA) kit (E-EL-R2466C, Elabscience Biotechnology Inc) to determine the optimal concentration and incubation time for inhibiting insulin secretion by Oc.

Techniques: Expressing, Immunofluorescence, Staining

Journal: Endocrine Oncology

Article Title: Paltusotine versus octreotide: different effects on radioligand uptake in neuroendocrine tumours

doi: 10.1530/EO-25-0041

Figure Lengend Snippet: (A) 18 F-SiTATE uptake assay using BON-1 SSTR2 cells after treatment with either paltusotine or octreotide. Cellular uptake was measured after (B) 30 min, (C) 120 min and (D) 240 min. DOTATATE acetate was used as the blocked control. The data are depicted as mean ± SD of four duplicate experiments. Molar concentrations: 100× (7.3–25.4 nM), 1,000× (73–254 nM), 2,000× (146–508 nM). Significantly lower radioligand uptake compared to the unblocked control * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Paltusotine and octreotide (both MedChemExpress, USA) were tested in concentrations of 4–500 nM.

Techniques: Control

Journal: Endocrine Oncology

Article Title: Paltusotine versus octreotide: different effects on radioligand uptake in neuroendocrine tumours

doi: 10.1530/EO-25-0041

Figure Lengend Snippet: Treatment of different (A) NET cell lines and (B) patient-derived GEP-NET primary cultures ( n = 14) with paltusotine and octreotide. Each NET cell line is depicted in a different colour. The data are depicted as mean ± SD including the individual data points. Significant increase/decrease in cell viability compared to control DMSO * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Paltusotine and octreotide (both MedChemExpress, USA) were tested in concentrations of 4–500 nM.

Techniques: Derivative Assay, Control

Journal: Endocrine Oncology

Article Title: Paltusotine versus octreotide: different effects on radioligand uptake in neuroendocrine tumours

doi: 10.1530/EO-25-0041

Figure Lengend Snippet: Individual patient-derived GEP-NET primary cultures after incubation with (A) paltusotine 100 and 500 nM or (B) octreotide 4 nM and 40 μM. panNETs are diamond-shaped and siNETs are circular-shaped. Each primary culture is shown in a different colour; metastatic tumours are depicted with a black frame. The data are depicted as mean ± SD including the individual data points.

Article Snippet: Paltusotine and octreotide (both MedChemExpress, USA) were tested in concentrations of 4–500 nM.

Techniques: Derivative Assay, Incubation

Journal: International Journal of Molecular Sciences

Article Title: Effects of Spermine Synthase Deficiency in Mesenchymal Stromal Cells Are Rescued by Upstream Inhibition of Ornithine Decarboxylase

doi: 10.3390/ijms25052463

Figure Lengend Snippet: Schematic overview of the polyamine synthesis pathway. Abbreviations: AMD1: Adenosylmethionine decarboxylase 1, ODC1: Ornithine decarboxylase, SRM: Spermidine synthase, SMS: Spermine synthase, SMOX: Spermine oxidase, SAT1: Spermidine/spermine N1-acetyltransferase, PAOX: Polyamine oxidase, DFMO: Difluoromethylornithine, CDAP: N-cyclohexyl-1,3-propanediamine.

Article Snippet: Reverse transcription was performed using TaqMan Reverse Transcription Reagents (Life Technologies, Grand Island, NY, USA), and mRNA was measured by real-time PCR using TaqMan Gene Expression Master Mix (Life Technologies, Carlsbad, CA, USA) with TaqMan probes for the following genes: ALPL (Hs01029144_m1), SPP1 (Hs00959010_m1), IBSP (Hs00913377_m1), ODC1 (Hs00159739_m1), SMS (Hs04998877_g1), SRM (Hs01027696_g1), AMD1 (Hs00750876_s1), SAT1 (Hs00971739_g1), SMOX (Hs00602494_m1), PAOX (Hs00382210_m1), ATF4 (Hs00909569_g1), ATF5 (Hs01119208_m1), CHOP (Hs00358796_g1), and GAPDH (Hs02786624_g1).

Techniques: