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Image Search Results
Journal: BioMed Research International
Article Title: Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering
doi: 10.1155/2021/9391575
Figure Lengend Snippet: Quantitative real-time PCR. SMC-specific contractile gene induction for calponin, smoothelin, and MyH11 is increased in early spheroids, particularly on day 4 of spheroid culture. Interestingly, comparison shows a stark contrast, with almost no changes in 2D SMC gene induction over time.
Article Snippet: Primers for smoothelin (
Techniques: Real-time Polymerase Chain Reaction, Comparison
Journal: BioMed Research International
Article Title: Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering
doi: 10.1155/2021/9391575
Figure Lengend Snippet: Immunofluorescent staining. SMC spheroids and 2D (monolayer) SMCs stained positive for SMC lineage-specific markers α -smooth muscle actin, calponin, smoothelin, and myosin heavy chain 11. Bladder wall ECM proteins collagen I, collagen III, elastin, and fibronectin are more strongly expressed in SMC spheroids. Nuclei stained blue with DAPI. Scale bar is 100 μ m.
Article Snippet: Primers for smoothelin (
Techniques: Staining
Journal: BioMed Research International
Article Title: Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering
doi: 10.1155/2021/9391575
Figure Lengend Snippet: Immunoblotting. The figure displays summarized representative bands and absolute protein concentrations normalized to GAPDH. Both SMC spheroids and 2D SMCs express the characteristic contractile marker proteins ( α -SMA, calponin, smoothelin, and MyH11). Immunoblotting indicates that SMC spheroids quickly shift to a more differentiated phenotype than do 2D SMCs. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. See figure for representative bands for all time points.
Article Snippet: Primers for smoothelin (
Techniques: Western Blot, Marker