Journal: iScience

Article Title: The role of long noncoding RNA Nron in atherosclerosis development and plaque stability

doi: 10.1016/j.isci.2022.103978

Figure Lengend Snippet:

Article Snippet: Primary antibodies specific for F4/80 (ab100790, Abcam), α-SMA (ab240654, Abcam) or VEGFA (ab1316, Abcam) was used.

Techniques: Virus, Recombinant, Plasmid Preparation, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Silver Staining, In Vitro, TUNEL Assay, Software

Journal: Biology direct

Article Title: RGS5 maintaining vascular homeostasis is altered by the tumor microenvironment.

doi: 10.1186/s13062-023-00437-y

Figure Lengend Snippet: Fig. 5 High RGS5 expression is associated with increased tumor vasculature in the patients with breast cancer. (A) Representative immunohistochemical staining for RGS5, α-SMA and CD34 expression in TNBC and adjacent normal tissues. Scale bar = 100 μm. Right: Quantification of the immunohistochemistry (n = 50). (B) Western blot and quantification of α-SMA and RGS5 in TNBC tissues. (C) Representative immunofluorescence images of RGS5 and α-SMA in TNBC tissues. Scale bar = 100 μm. The data were presented as the mean ± SD

Article Snippet: After blocking with 5% milk in TBST, the membranes were incubated with primary antibodies against VCAM-1 (1:1000, Epitomics), RGS5 (1:500, Proteintech), MCP-1(1:200, Santa Cruz), C3 (1:250, Abcam), CD74 (1:500, Abcam), Lyz2 (1:200, Proteintech) α-SMA(1:1000, Proteintech), Caspase-1 (1:200, Proteintech), GSDMD (1:200, Abcam) or β-actin (1:1000, Proteintech) at 4°C overnight, and then with the HRPconjugated secondary antibody for 1 h. The blots were visualized using Image Quant LAS 4000 detection system (GE Healthcare).

Techniques: Expressing, Immunohistochemical staining, Staining, Immunohistochemistry, Western Blot, Immunofluorescence

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: Actin (smooth muscle) , REAL650 , 50 , 130-123-363 , FITC , Miltenyi Biotec.

Techniques: Imaging

Journal: Scientific Reports

Article Title: The antifibrotic effect of Vildagliptin and Diaminodiphenyl Sulfone in murine schistosomiasis mansoni

doi: 10.1038/s41598-025-91955-4

Figure Lengend Snippet: Effect of Vilda, DDS, and Vilda/DDS on α-SMA and MMP-9 as fibrotic markers on S. mansoni- infected mice. ( a ) Photomicrographs of immunohistochemical staining (IHC) of hepatic tissues with alpha-smooth muscle actin (α-SMA) (× 200, scale bar = 100µm) as follows: liver sections of normal control and normal treated groups (Control, Vilda, DDS, and Vilda/DDS) marked scarce hepatocytes with positive cytoplasmic reactivity to α-SMA antibody (arrow). Liver sections from (Infected to Inf + PZQ) highlighted positive expression of α-SMA along ova of Schistosoma (arrowhead), fibroblast encircling granuloma (circle), inflammatory cells with nuclear expression (cube), and surrounding hepatocytes with cytoplasmic reactivity (arrow). The expressions were graded as intense in the Infected group, high in Inf + DDS group , moderately high in Inf + PZQ group , moderately low in Inf + Vilda/DDS group , and few in Inf + Vilda group. ( b ) Quantitative analysis of hepatic α-SMA IHC expression; (c) Hepatic level of matrix metallopeptidase-9 (MMP-9) as a fibrotic marker . Data are presented as mean ± SEM, (n = 6) and analyzed by one-way ANOVA followed by Tukey multiple comparisons. a Significantly different from the normal untreated group (Control), b Significantly different from the S. mansoni -infected untreated group (infected), c Significantly different from Inf + PZQ group, and d Significantly different from Inf + Vilda/DDS group, at P < 0.05. Inf + PZO, infected mice treated with paraziquantel; Inf + Vilda/DDS, infected mice treated with a combination of vildagliptin and diaminodiphenyl sulphone; Inf + Vilda, infected mice treated with vildagliptin; Inf + DDS, infected mice treated with diaminodiphenyl sulphone. All treatments were administered for 14 consecutive days, except PZQ for two consecutive days.

Article Snippet: Regarding immunohistochemical examination, thick deparaffinized liver Sects. (3–5 μm) were evaluated for the immunohistochemical reactivity of Anti-Rabbit α-SMA polyclonal Antibody (Cat#E-AB-34268, Elabscience, USA) and Anti-TGF-beta-1 polyclonal Antibody (Cat#ARG56429, Ari Gobio), following the manufacturer’s procedure.

Techniques: Infection, Immunohistochemical staining, Staining, Control, Expressing, Marker