Journal: Cancer Cell International

Article Title: KHDRBS3 accelerates glycolysis and promotes malignancy of hepatocellular carcinoma via upregulating 14-3-3ζ

doi: 10.1186/s12935-023-03085-4

Figure Lengend Snippet: Sequence of primers for qRT-PCR

Article Snippet: Slides were blocked in 1% BSA (Sangon, Shanghai, China) for 15 min and then incubated with KHDRBS3 antibody (Santa Cruz Biotechnology, sc-374,461, Shanghai, China) diluted (1:50) in PBS at 4 °C overnight.

Techniques: Sequencing

Journal: Cancer Cell International

Article Title: KHDRBS3 accelerates glycolysis and promotes malignancy of hepatocellular carcinoma via upregulating 14-3-3ζ

doi: 10.1186/s12935-023-03085-4

Figure Lengend Snippet: KHDRBS3 is elevated in human HCC tissues and is associated with poor clinal outcomes. ( A ) The expression profiles of KHDRBS3 gene in HCC on GEPIA database. ( B ) The expression of KHDRBS3 gene in three GEO datasets. ( C ) The overall survival (OS), recurrence-free survival (RFS), progression-free survival (PFS) and disease-specific survival (DSS) analysis in HCC patients with different KHDRBS3 expression. ( D-E ) KHDRBS3 expression in human HCC and adjacent tissues were detected by qPCR and Western blotting. Error bars represent standard deviation. * P < 0.05 vs. Para-tumor group; ** P < 0.01 vs. Normal group

Article Snippet: Slides were blocked in 1% BSA (Sangon, Shanghai, China) for 15 min and then incubated with KHDRBS3 antibody (Santa Cruz Biotechnology, sc-374,461, Shanghai, China) diluted (1:50) in PBS at 4 °C overnight.

Techniques: Expressing, Western Blot, Standard Deviation

Journal: Cancer Cell International

Article Title: KHDRBS3 accelerates glycolysis and promotes malignancy of hepatocellular carcinoma via upregulating 14-3-3ζ

doi: 10.1186/s12935-023-03085-4

Figure Lengend Snippet: Representative images of KHDRBS3 immunohistochemical staining in HCC and adjacent tissues

Article Snippet: Slides were blocked in 1% BSA (Sangon, Shanghai, China) for 15 min and then incubated with KHDRBS3 antibody (Santa Cruz Biotechnology, sc-374,461, Shanghai, China) diluted (1:50) in PBS at 4 °C overnight.

Techniques: Immunohistochemical staining, Staining

Journal: Cancer Cell International

Article Title: KHDRBS3 accelerates glycolysis and promotes malignancy of hepatocellular carcinoma via upregulating 14-3-3ζ

doi: 10.1186/s12935-023-03085-4

Figure Lengend Snippet: KHDRBS3 promotes cell proliferation in vitro and tumor growth in vivo. ( A ) The efficiency of knockdown and overexpression of KHDRBS3 was examined by Western blotting analysis. ( B-C ) MTT cell proliferation assay and colony formation assay in indicated cells. ( D-F ) Image, volume and weights of xenograft tumors derived from the Huh7 cells. ( G ) The protein levels of KHDRBS3 expression in xenograft tumors. Error bars represent standard deviation. ** P < 0.01 vs. Vector group or NC − sh group

Article Snippet: Slides were blocked in 1% BSA (Sangon, Shanghai, China) for 15 min and then incubated with KHDRBS3 antibody (Santa Cruz Biotechnology, sc-374,461, Shanghai, China) diluted (1:50) in PBS at 4 °C overnight.

Techniques: In Vitro, In Vivo, Knockdown, Over Expression, Western Blot, MTT Cell Proliferation, Colony Assay, Derivative Assay, Expressing, Standard Deviation, Plasmid Preparation

Journal: Cancer Cell International

Article Title: KHDRBS3 accelerates glycolysis and promotes malignancy of hepatocellular carcinoma via upregulating 14-3-3ζ

doi: 10.1186/s12935-023-03085-4

Figure Lengend Snippet: The knockdown of KHDRBS3 induces apoptosis and inhibits glycolysis of HCC cells. ( A ) Apoptosis was analyzed using flow cytometry with Annexin V-PI staining double staining. ( B-C ) Measurement of glucose consumption and lactate production in cell culture medium. Error bars represent standard deviation. * P < 0.05, ** P < 0.01 vs. NC − sh group

Article Snippet: Slides were blocked in 1% BSA (Sangon, Shanghai, China) for 15 min and then incubated with KHDRBS3 antibody (Santa Cruz Biotechnology, sc-374,461, Shanghai, China) diluted (1:50) in PBS at 4 °C overnight.

Techniques: Knockdown, Flow Cytometry, Staining, Double Staining, Cell Culture, Standard Deviation

Journal: Cancer Cell International

Article Title: KHDRBS3 accelerates glycolysis and promotes malignancy of hepatocellular carcinoma via upregulating 14-3-3ζ

doi: 10.1186/s12935-023-03085-4

Figure Lengend Snippet: KHDRBS3 enhances chemoresistance of HCC cells. ( A ) Cell viability of HCC cells upon treatment with different concentrations of doxorubicin was assessed by MTT. ( B ) Flow cytometry analysis of indicated cells with doxorubicin (1 µmol) treatment. Error bars represent standard deviation. ** P < 0.01 vs. Vector group or NC − sh group

Article Snippet: Slides were blocked in 1% BSA (Sangon, Shanghai, China) for 15 min and then incubated with KHDRBS3 antibody (Santa Cruz Biotechnology, sc-374,461, Shanghai, China) diluted (1:50) in PBS at 4 °C overnight.

Techniques: Flow Cytometry, Standard Deviation, Plasmid Preparation

Journal: Cancer Cell International

Article Title: KHDRBS3 accelerates glycolysis and promotes malignancy of hepatocellular carcinoma via upregulating 14-3-3ζ

doi: 10.1186/s12935-023-03085-4

Figure Lengend Snippet: KHDRBS3 binds to YWHAZ and upregulates its expression. ( A ) Relative mRNA levels of c-Myc, GLUT1, LDHA, YWHAZ, HOXB9, CRABP1 and DLL4 in stable KHDRBS3 knockdown or overexpressing Huh7 cells. ( B ) Protein levels of c-Myc, GLUT1, LDHA and 14-3-3ζ in infected Huh7 cells were evaluated by western blotting. ( C ) RIP assay was performed to detect the enrichment of c-Myc, GLUT1, LDHA and YWHAZ in the anti-KHDRBS3 IP group. ( D ) RNA pull-down assay determined the binding between KHDRBS3 and YWHAZ. Error bars represent standard deviation. ** P < 0.01 vs. Vector group or NC − sh group

Article Snippet: Slides were blocked in 1% BSA (Sangon, Shanghai, China) for 15 min and then incubated with KHDRBS3 antibody (Santa Cruz Biotechnology, sc-374,461, Shanghai, China) diluted (1:50) in PBS at 4 °C overnight.

Techniques: Expressing, Knockdown, Infection, Western Blot, Pull Down Assay, Binding Assay, Standard Deviation, Plasmid Preparation

Journal: Cancer Cell International

Article Title: KHDRBS3 accelerates glycolysis and promotes malignancy of hepatocellular carcinoma via upregulating 14-3-3ζ

doi: 10.1186/s12935-023-03085-4

Figure Lengend Snippet: KHDRBS3 promotes malignant progression of HCC cells via regulating 14-3-3ζ expression. ( A ) 14-3-3ζ protein levels in Huh7 cells were measured by western blotting. ( B ) Cell proliferation was assessed using MTT assay. ( C ) Cell apoptosis was analyzed by flow cytometry. ( D-E ) Glucose consumption and lactate production were measured in Huh7 cells. Error bars represent standard deviation. * P < 0.05, ** P < 0.01 vs. KHDRBS3 + NC − si group; # P < 0.05, ## P < 0.01 vs. NC − si group

Article Snippet: Slides were blocked in 1% BSA (Sangon, Shanghai, China) for 15 min and then incubated with KHDRBS3 antibody (Santa Cruz Biotechnology, sc-374,461, Shanghai, China) diluted (1:50) in PBS at 4 °C overnight.

Techniques: Expressing, Western Blot, MTT Assay, Flow Cytometry, Standard Deviation

Journal: Cancer Cell International

Article Title: KHDRBS3 accelerates glycolysis and promotes malignancy of hepatocellular carcinoma via upregulating 14-3-3ζ

doi: 10.1186/s12935-023-03085-4

Figure Lengend Snippet: Diagram of the mechanism of KHDRBS3 promoting HCC progression. KHDRBS3 upregulates 14-3-3ζ expression by binding to YWHAZ, resulting in promotion of glycolysis, cell proliferation, chemoresistance and inhibition of apoptosis

Article Snippet: Slides were blocked in 1% BSA (Sangon, Shanghai, China) for 15 min and then incubated with KHDRBS3 antibody (Santa Cruz Biotechnology, sc-374,461, Shanghai, China) diluted (1:50) in PBS at 4 °C overnight.

Techniques: Expressing, Binding Assay, Inhibition