slc5a5 Search Results


gene exp slc5a5 hs00166567 m1  (Thermo Fisher)
90
Thermo Fisher gene exp slc5a5 hs00166567 m1
Gene Exp Slc5a5 Hs00166567 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti nis  (Novus Biologicals)
92
Novus Biologicals anti nis
Anti Nis, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp slc5a5 rn00583900 m1  (Thermo Fisher)
95
Thermo Fisher gene exp slc5a5 rn00583900 m1
Gene Exp Slc5a5 Rn00583900 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp slc5a5 hs00950362 g1  (Thermo Fisher)
86
Thermo Fisher gene exp slc5a5 hs00950362 g1
Gene Exp Slc5a5 Hs00950362 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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anti nis  (Proteintech)
94
Proteintech anti nis
FIGURE 2 | hsa_circ_0023990 regulates <t>NIS</t> expression through affecting its methylation level. (A, B) <t>The</t> <t>DNMT1</t> and NIS protein expressions in thyroid cancer tissues and paracancerous tissues were detected by western blot. (C) The NIS promoter methylation in RAIR-DTC/paracancer- ous tissues was detected by MSP assay. TPC1 cells and KMH-2 cells were transfected with hsa_circ_0023990 overexpression plasmid and si-hsa_ circ_0023990, respectively. (D–G) The DNMT1 mRNA and protein expressions in cells were detected by RT-PCR and western blot. (H–K) The NIS mRNA and protein expressions in cells were detected by RT-PCR and western blot. (L) The NIS promoter methylation in cells was detected by MSP assay. **p < 0.01, ***p < 0.001.
Anti Nis, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp slc5a5 ss03394915 m1  (Thermo Fisher)
93
Thermo Fisher gene exp slc5a5 ss03394915 m1
FIGURE 2 | hsa_circ_0023990 regulates <t>NIS</t> expression through affecting its methylation level. (A, B) <t>The</t> <t>DNMT1</t> and NIS protein expressions in thyroid cancer tissues and paracancerous tissues were detected by western blot. (C) The NIS promoter methylation in RAIR-DTC/paracancer- ous tissues was detected by MSP assay. TPC1 cells and KMH-2 cells were transfected with hsa_circ_0023990 overexpression plasmid and si-hsa_ circ_0023990, respectively. (D–G) The DNMT1 mRNA and protein expressions in cells were detected by RT-PCR and western blot. (H–K) The NIS mRNA and protein expressions in cells were detected by RT-PCR and western blot. (L) The NIS promoter methylation in cells was detected by MSP assay. **p < 0.01, ***p < 0.001.
Gene Exp Slc5a5 Ss03394915 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nis antibody conjugated with af488  (Novus Biologicals)
91
Novus Biologicals nis antibody conjugated with af488
FIGURE 2 | hsa_circ_0023990 regulates <t>NIS</t> expression through affecting its methylation level. (A, B) <t>The</t> <t>DNMT1</t> and NIS protein expressions in thyroid cancer tissues and paracancerous tissues were detected by western blot. (C) The NIS promoter methylation in RAIR-DTC/paracancer- ous tissues was detected by MSP assay. TPC1 cells and KMH-2 cells were transfected with hsa_circ_0023990 overexpression plasmid and si-hsa_ circ_0023990, respectively. (D–G) The DNMT1 mRNA and protein expressions in cells were detected by RT-PCR and western blot. (H–K) The NIS mRNA and protein expressions in cells were detected by RT-PCR and western blot. (L) The NIS promoter methylation in cells was detected by MSP assay. **p < 0.01, ***p < 0.001.
Nis Antibody Conjugated With Af488, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp slc5a5 mm01351811 m1  (Thermo Fisher)
86
Thermo Fisher gene exp slc5a5 mm01351811 m1
FIGURE 2 | hsa_circ_0023990 regulates <t>NIS</t> expression through affecting its methylation level. (A, B) <t>The</t> <t>DNMT1</t> and NIS protein expressions in thyroid cancer tissues and paracancerous tissues were detected by western blot. (C) The NIS promoter methylation in RAIR-DTC/paracancer- ous tissues was detected by MSP assay. TPC1 cells and KMH-2 cells were transfected with hsa_circ_0023990 overexpression plasmid and si-hsa_ circ_0023990, respectively. (D–G) The DNMT1 mRNA and protein expressions in cells were detected by RT-PCR and western blot. (H–K) The NIS mRNA and protein expressions in cells were detected by RT-PCR and western blot. (L) The NIS promoter methylation in cells was detected by MSP assay. **p < 0.01, ***p < 0.001.
Gene Exp Slc5a5 Mm01351811 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp slc5a5 hs00950356 m1  (Thermo Fisher)
90
Thermo Fisher gene exp slc5a5 hs00950356 m1
FIGURE 2 | hsa_circ_0023990 regulates <t>NIS</t> expression through affecting its methylation level. (A, B) <t>The</t> <t>DNMT1</t> and NIS protein expressions in thyroid cancer tissues and paracancerous tissues were detected by western blot. (C) The NIS promoter methylation in RAIR-DTC/paracancer- ous tissues was detected by MSP assay. TPC1 cells and KMH-2 cells were transfected with hsa_circ_0023990 overexpression plasmid and si-hsa_ circ_0023990, respectively. (D–G) The DNMT1 mRNA and protein expressions in cells were detected by RT-PCR and western blot. (H–K) The NIS mRNA and protein expressions in cells were detected by RT-PCR and western blot. (L) The NIS promoter methylation in cells was detected by MSP assay. **p < 0.01, ***p < 0.001.
Gene Exp Slc5a5 Hs00950356 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp slc5a5 rn01420249 g1  (Thermo Fisher)
85
Thermo Fisher gene exp slc5a5 rn01420249 g1
FIGURE 2 | hsa_circ_0023990 regulates <t>NIS</t> expression through affecting its methylation level. (A, B) <t>The</t> <t>DNMT1</t> and NIS protein expressions in thyroid cancer tissues and paracancerous tissues were detected by western blot. (C) The NIS promoter methylation in RAIR-DTC/paracancer- ous tissues was detected by MSP assay. TPC1 cells and KMH-2 cells were transfected with hsa_circ_0023990 overexpression plasmid and si-hsa_ circ_0023990, respectively. (D–G) The DNMT1 mRNA and protein expressions in cells were detected by RT-PCR and western blot. (H–K) The NIS mRNA and protein expressions in cells were detected by RT-PCR and western blot. (L) The NIS promoter methylation in cells was detected by MSP assay. **p < 0.01, ***p < 0.001.
Gene Exp Slc5a5 Rn01420249 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 2 | hsa_circ_0023990 regulates NIS expression through affecting its methylation level. (A, B) The DNMT1 and NIS protein expressions in thyroid cancer tissues and paracancerous tissues were detected by western blot. (C) The NIS promoter methylation in RAIR-DTC/paracancer- ous tissues was detected by MSP assay. TPC1 cells and KMH-2 cells were transfected with hsa_circ_0023990 overexpression plasmid and si-hsa_ circ_0023990, respectively. (D–G) The DNMT1 mRNA and protein expressions in cells were detected by RT-PCR and western blot. (H–K) The NIS mRNA and protein expressions in cells were detected by RT-PCR and western blot. (L) The NIS promoter methylation in cells was detected by MSP assay. **p < 0.01, ***p < 0.001.

Journal: Cancer science

Article Title: Hypoxia Inhibitor Improves Iodine Uptake Disorder in Thyroid Cancer Through the hsa_circ_0023990/miR-448/DNMT1/NIS Axis.

doi: 10.1111/cas.70102

Figure Lengend Snippet: FIGURE 2 | hsa_circ_0023990 regulates NIS expression through affecting its methylation level. (A, B) The DNMT1 and NIS protein expressions in thyroid cancer tissues and paracancerous tissues were detected by western blot. (C) The NIS promoter methylation in RAIR-DTC/paracancer- ous tissues was detected by MSP assay. TPC1 cells and KMH-2 cells were transfected with hsa_circ_0023990 overexpression plasmid and si-hsa_ circ_0023990, respectively. (D–G) The DNMT1 mRNA and protein expressions in cells were detected by RT-PCR and western blot. (H–K) The NIS mRNA and protein expressions in cells were detected by RT-PCR and western blot. (L) The NIS promoter methylation in cells was detected by MSP assay. **p < 0.01, ***p < 0.001.

Article Snippet: These membranes were subsequently incubated with primary antibodies, such as anti- DNMT1 (24206- 1- AP), anti- NIS (24324- 1- AP), and GAPDH (60004- 1- lg; Proteintech) at the recommended concentration overnight at 4°C.

Techniques: Expressing, Methylation, Western Blot, MSP Assay, Transfection, Over Expression, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction

FIGURE 6 | Hypoxia inhibitors increase iodine uptake in tumor tissue through the has_circ_0023990/DNMT1/NIS signaling axis to inhibit tu- mor growth. (A) H&E staining. Scale bar = 50 μm. (B, C) The cell proliferation in tumor tissues was detected by Ki-67 immunohistochemistry. “IOD/ Area” indicating the mean optical density of Ki-67 expression. Scale bar = 50 μm. (D, E) The cell apoptosis in tumor tissues was detected by TUNEL staining. Scale bar = 50 μm. (F) The hsa_circ_0023990 expression in tumor tissues was detected by RT-PCR. (G–I) The DNMT1 and NIS protein ex- pressions in tumor tissues were detected by western blot. *p < 0.05, **p < 0.01, ***p < 0.001.

Journal: Cancer science

Article Title: Hypoxia Inhibitor Improves Iodine Uptake Disorder in Thyroid Cancer Through the hsa_circ_0023990/miR-448/DNMT1/NIS Axis.

doi: 10.1111/cas.70102

Figure Lengend Snippet: FIGURE 6 | Hypoxia inhibitors increase iodine uptake in tumor tissue through the has_circ_0023990/DNMT1/NIS signaling axis to inhibit tu- mor growth. (A) H&E staining. Scale bar = 50 μm. (B, C) The cell proliferation in tumor tissues was detected by Ki-67 immunohistochemistry. “IOD/ Area” indicating the mean optical density of Ki-67 expression. Scale bar = 50 μm. (D, E) The cell apoptosis in tumor tissues was detected by TUNEL staining. Scale bar = 50 μm. (F) The hsa_circ_0023990 expression in tumor tissues was detected by RT-PCR. (G–I) The DNMT1 and NIS protein ex- pressions in tumor tissues were detected by western blot. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: These membranes were subsequently incubated with primary antibodies, such as anti- DNMT1 (24206- 1- AP), anti- NIS (24324- 1- AP), and GAPDH (60004- 1- lg; Proteintech) at the recommended concentration overnight at 4°C.

Techniques: Staining, Immunohistochemistry, Expressing, TUNEL Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot