Journal: Biology of Sex Differences

Article Title: Gestational saccharin consumption disrupts gut-brain axis glucose homeostasis control in adolescent offspring rats in a sex-dependent manner

doi: 10.1186/s13293-025-00724-5

Figure Lengend Snippet: Glucose homeostasis gene and protein expression in adolescent offspring small intestine. Study of gene expression of Glp1r ( a ) , Tas1r3 ( d ) , Pyy ( f ) , Cd36 ( g ) , Slc5a1 ( i ) , Gcg ( j ) by qPCR (n = 9 for each experimental group) and protein expression of GLP1 ( b ), GLPR1 ( c ), TAS1R3 ( e ), CD36 ( h ) by Western blot (n = 6 for each experimental group) in the small intestine of male and female adolescent offspring. ( k ) Representative blots immunostained for each of the proteins tested. Data are presented as mean ± SEM and analyzed by two-way ANOVA. Significant post-hoc analysis is shown: *p < 0.05, ***p < 0.001 versus male within the same perinatal condition; ##p < 0.01 versus control within the same sex

Article Snippet: Slc5a1 , Rn01640634_m1 , 64.

Techniques: Expressing, Gene Expression, Western Blot, Control

Journal: Cardiovascular Diabetology

Article Title: Characterization of left ventricular myocardial sodium-glucose cotransporter 1 expression in patients with end-stage heart failure

doi: 10.1186/s12933-020-01141-1

Figure Lengend Snippet: Myocardial left ventricular expression of the three major glucose transporters and their correlations. a Quantification of left ventricular (LV) relative mRNA expression of sodium-glucose cotransporter 1 (SGLT1) in controls and in patients with heart failure (HF). b Quantification of LV relative mRNA expression of facilitative glucose transporter 1 (GLUT1) in controls and in patients with HF. c Quantification of LV relative mRNA expression of GLUT4 in controls and in patients with HF. d Correlation between the LV relative mRNA expressions of SGLT1 and GLUT1. e Correlation between the LV relative mRNA expressions of SGLT1 and GLUT4. f Correlation between the LV relative mRNA expressions of GLUT1 and GLUT4. For easier interpretation, expression values are normalized to that of Controls (i.e. mean of Control group = 1.00). To compare means of five groups, one-way analysis of variance (ANOVA) was performed with Welch’s correction followed by Dunnett T3 post hoc test to compute intergroup differences relative to the Control group. Statistical significance according to the post hoc test (relative to Control) is highlighted as follows: *P < 0.05, **P < 0.01. For zero-order correlation analysis, Spearman’s rho (r s ) was computed. For all correlation coefficients, bias-corrected and accelerated (BCa) 95% confidence intervals (CI) are reported based on n = 1000 bootstrap samples. DCM dilated cardiomyopathy, f. c. fold change, GLUT1 and 4 facilitative glucose transporter 1 and 4, HCM hypertrophic cardiomyopathy, IHD ischemic heart disease, IHD-T2DM IHD and type 2 diabetes mellitus, r s Spearman’s rho (correlation coefficient), SGLT1 sodium-glucose cotransporter 1

Article Snippet: Then, quantitative real-time polymerase chain reaction (qRT-PCR) was performed on StepOnePlus RT PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using TaqMan Universal PCR MasterMix and TaqMan Gene Expression Assays (Thermo Fisher Scientific) for the following targets: Solute Carrier Family 5 Member 1 (SLC5A1 encoding SGLT1; ID: Hs01573793_m1); SLC5A2 (encoding SGLT2; ID: Hs00894642_m1); SLC2A1 (encoding GLUT1; ID: Hs00892681_m1); SLC2A4 (encoding GLUT4; ID: Hs00168966_m1); and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; ID: Hs99999905_m1).

Techniques: Expressing

Journal: Cardiovascular Diabetology

Article Title: Characterization of left ventricular myocardial sodium-glucose cotransporter 1 expression in patients with end-stage heart failure

doi: 10.1186/s12933-020-01141-1

Figure Lengend Snippet: Results of statistical analyses correcting for age, sex, and body mass index (BMI)

Article Snippet: Then, quantitative real-time polymerase chain reaction (qRT-PCR) was performed on StepOnePlus RT PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using TaqMan Universal PCR MasterMix and TaqMan Gene Expression Assays (Thermo Fisher Scientific) for the following targets: Solute Carrier Family 5 Member 1 (SLC5A1 encoding SGLT1; ID: Hs01573793_m1); SLC5A2 (encoding SGLT2; ID: Hs00894642_m1); SLC2A1 (encoding GLUT1; ID: Hs00892681_m1); SLC2A4 (encoding GLUT4; ID: Hs00168966_m1); and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; ID: Hs99999905_m1).

Techniques: Expressing

Journal: Cardiovascular Diabetology

Article Title: Characterization of left ventricular myocardial sodium-glucose cotransporter 1 expression in patients with end-stage heart failure

doi: 10.1186/s12933-020-01141-1

Figure Lengend Snippet: Protein expression of SGLT1, and activation of ERK1/2 and AMPKα. a Relative protein expression of left ventricular (LV) sodium-glucose cotransporter 1 (SGLT1) in controls and in patients with heart failure (HF). A representative blot is shown. b The ratio of LV phosphorylated extracellular signal-regulated kinase 1/2 (P-ERK1/2) versus total ERK1/2 (t-ERK1/2) in controls and in patients with HF. A representative blot is shown. c The ratio of LV phosphorylated adenosine-monophosphate-activated protein kinase α catalytic subunit (P-AMPKα) versus total AMPKα (t-AMPKα) in controls and in patients with HF. A representative blot is shown. d Correlation between LV end-diastolic diameter (LVEDD), ejection fraction (EF) and SGLT1 relative protein expression. e Correlation between LVEDD, EF and P-ERK1/2 / t-ERK1/2 relative protein expression. f Correlation between LVEDD, EF and P-AMPKα / t- AMPKα relative protein expression. g Correlation between P-ERK1/2 / t-ERK1/2 and SGLT1 relative protein expressions. h Correlation between P- AMPKα / t-AMPKα and SGLT1 relative protein expressions. For easier interpretation, expression values are normalized to that of Controls (i.e. mean of Control group = 1.00). To compare means of five groups, one-way analysis of variance (ANOVA) was performed with Welch’s correction followed by Dunnett T3 post hoc test to compute intergroup differences relative to the Control group. Statistical significance according to the post hoc test (relative to Control) is highlighted as follows: *P < 0.05, **P < 0.01. For zero-order correlation analysis, Spearman’s rho (r s ) was computed. For all correlation coefficients, bias-corrected and accelerated (BCa) 95% confidence intervals (CI) are reported based on n = 1000 bootstrap samples. AMPKα adenosine-monophosphate-activated protein kinase α catalytic subunit, DCM dilated cardiomyopathy, EF ejection fraction, ERK1/2 extracellular signal-regulated kinase 1/2, f. c. fold change, HCM hypertrophic cardiomyopathy, LVEDD left ventricular end-diastolic diameter, IHD ischemic heart disease, IHD-T2DM IHD and type 2 diabetes mellitus, r s Spearman’s rho (correlation coefficient), SGLT1 sodium-glucose cotransporter 1

Article Snippet: Then, quantitative real-time polymerase chain reaction (qRT-PCR) was performed on StepOnePlus RT PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using TaqMan Universal PCR MasterMix and TaqMan Gene Expression Assays (Thermo Fisher Scientific) for the following targets: Solute Carrier Family 5 Member 1 (SLC5A1 encoding SGLT1; ID: Hs01573793_m1); SLC5A2 (encoding SGLT2; ID: Hs00894642_m1); SLC2A1 (encoding GLUT1; ID: Hs00892681_m1); SLC2A4 (encoding GLUT4; ID: Hs00168966_m1); and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; ID: Hs99999905_m1).

Techniques: Expressing, Activation Assay

Journal: Cardiovascular Diabetology

Article Title: Characterization of left ventricular myocardial sodium-glucose cotransporter 1 expression in patients with end-stage heart failure

doi: 10.1186/s12933-020-01141-1

Figure Lengend Snippet: Immunohistochemical analysis of SGLT1 in the heart. a Representative left ventricular (LV) epicardial section from a patient with dilated cardiomyopathy (DCM). The brownish staining refers to sodium-glucose cotransporter 1 (SGLT1). Full arrows indicate fibrotic tissue, blank arrows indicate adipose tissue, whereas the asterisk marks the lumen of an epicardial vessel. b Representative LV sections from controls and from patients with heart failure (HF). The brownish staining refers to SGLT1. Full arrows indicate fibrotic tissue, whereas blank arrows indicate adipose tissue. c Representative LV immunofluorescent sections from a patient with DCM depicting SGLT1 in green, the sarcolemma marker sodium–potassium ATPase (Na–K-ATPase) in red, and nuclei (DAPI) in blue. A merge of these recordings is also shown. Full arrows indicate staining of SGLT1 and Na–K-ATPase, respectively. DAPI 4′,6-diamidino-2-phenylindole, DCM dilated cardiomyopathy, HCM hypertrophic cardiomyopathy, IHD ischemic heart disease, IHD-T2DM IHD and type 2 diabetes mellitus, Na–K-ATPase sodium–potassium ATPase, SGLT1 sodium-glucose cotransporter 1

Article Snippet: Then, quantitative real-time polymerase chain reaction (qRT-PCR) was performed on StepOnePlus RT PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using TaqMan Universal PCR MasterMix and TaqMan Gene Expression Assays (Thermo Fisher Scientific) for the following targets: Solute Carrier Family 5 Member 1 (SLC5A1 encoding SGLT1; ID: Hs01573793_m1); SLC5A2 (encoding SGLT2; ID: Hs00894642_m1); SLC2A1 (encoding GLUT1; ID: Hs00892681_m1); SLC2A4 (encoding GLUT4; ID: Hs00168966_m1); and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; ID: Hs99999905_m1).

Techniques: Immunohistochemical staining, Staining, Marker

Journal: Cardiovascular Diabetology

Article Title: Characterization of left ventricular myocardial sodium-glucose cotransporter 1 expression in patients with end-stage heart failure

doi: 10.1186/s12933-020-01141-1

Figure Lengend Snippet: Effect of cardiac resynchronization therapy on left ventricular expression of the three major glucose transporters. a Comparison of left ventricular (LV) relative mRNA expression of sodium-glucose cotransporter 1 (SGLT1) between heart failure (HF) patients with and without cardiac resynchronization therapy (CRT). b Comparison of LV relative mRNA expression of facilitative glucose transporter 1 (GLUT1) between HF patients with and without CRT. c Comparison of LV relative mRNA expression of GLUT4 between HF patients with and without CRT. d Relative protein expression of LV SGLT1 in patients with dilated cardiomyopathy (DCM) with and without CRT. A representative blot is depicted. e Phosphorylated extracellular signal-regulated kinase 1/2 (P-ERK1/2) versus total ERK1/2 (t-ERK1/2) protein expressions in patients with DCM with and without CRT. A representative blot is depicted. For easier interpretation, expression values are normalized to that of non-CRT groups within each etiological subgroup (i.e. mean of corresponding non-CRT group = 1.00). Point-biserial correlation analysis on ranked scores was performed to compute the overall effect of CRT on LV mRNA expression of target genes, the related P values are reported. Significance of difference between two groups was assessed using unpaired Student t -test with Welch’s correction. Accordingly, statistical significance is highlighted as: *P < 0.05. a.u. arbitrary units, CRT cardiac resynchronization therapy, DCM dilated cardiomyopathy, f. c. fold change, ERK1/2 extracellular signal-regulated kinase 1/2, GAPDH glyceraldehyde-3-phosphate dehydrogenase, GLUT1 and 4 facilitative glucose transporter 1 and 4, HCM hypertrophic cardiomyopathy, IHD ischemic heart disease, IHD-T2DM IHD and type 2 diabetes mellitus, SGLT1 sodium-glucose cotransporter 1

Article Snippet: Then, quantitative real-time polymerase chain reaction (qRT-PCR) was performed on StepOnePlus RT PCR System (Thermo Fisher Scientific, Waltham, MA, USA) using TaqMan Universal PCR MasterMix and TaqMan Gene Expression Assays (Thermo Fisher Scientific) for the following targets: Solute Carrier Family 5 Member 1 (SLC5A1 encoding SGLT1; ID: Hs01573793_m1); SLC5A2 (encoding SGLT2; ID: Hs00894642_m1); SLC2A1 (encoding GLUT1; ID: Hs00892681_m1); SLC2A4 (encoding GLUT4; ID: Hs00168966_m1); and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; ID: Hs99999905_m1).

Techniques: Expressing

Journal: Scientific Reports

Article Title: Concise and Stereodivergent Synthesis of Carbasugars Reveals Unexpected Structure-Activity Relationship (SAR) of SGLT2 Inhibition

doi: 10.1038/s41598-017-05895-9

Figure Lengend Snippet: IC 50 values for the novel analogues of dapagliflozin towards SGLT1 and SGLT2.

Article Snippet: SGLT1- or SGLT2- expressing cells (S1 or S2) were developed by transfecting, respectively, SLC5A1 (NM_000343) or SLC5A2 (NM_003041) human cDNA clone (Origene Technologies Inc., Maryland, USA) into COS-7 cells (monkey fibroblast-like kidney cells, CRL-1651, ATCC, Manassas, USA).

Techniques: