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Image Search Results
Journal: Frontiers in Nutrition
Article Title: High-fat diet mouse model receiving L-glucose supplementations propagates liver injury
doi: 10.3389/fnut.2024.1469952
Figure Lengend Snippet: Metabolic profile assessment: (A) Fasting blood sugar (FBS) was assessed as indicated in materials and methods. GLUT2 expressions were quantitated by (B) western blot and (C) RT-PCR methods. (D) Serum insulin C-peptide measured by ELISA. (E) Hepatic expression of inulin receptor (IR) was evaluated from liver sections by western blot (F) HOMA2 calculator calculated HOMA-IR. Paired and unpaired Student’s t -test and ANOVA were used for statistically significant differences. p value was compared between RD and HFD control groups or within RD or HFD groups. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.
Article Snippet: Rabbit anti-Human/Mouse GYS2 (Proteintech, 22371-1-AP), Rabbit anti-Human/Mouse Glycogen synthase [p Ser641] (Novus bio, NBP2-67315), rabbit anti-human/mouse PYGL antibody (Proteintech, 15851-1-AP), rabbit
Techniques: Western Blot, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Expressing, Control
Journal: Diabetes, obesity & metabolism
Article Title: Caffeic acid, naringenin and quercetin enhance glucose-stimulated insulin secretion and glucose sensitivity in INS-1E cells.
doi: 10.1111/dom.12236
Figure Lengend Snippet: Figure 4. mRNA abundance of Glut2, Gck, Ins1, Ins2, Beta2 and Pdx1 in INS-1E cells treated with caffeic acid, naringenin or quercetin in presence of 11 or 25 mM glucose for 72 h were studied by real time RT-PCR using TaqMan® assays (ABI, Foster City, CA, USA). Duplicate samples were taken for each treatment and the samples were measured in triplicates. Gene expressions were normalized to 18 S ribosomal RNA. Difference in the mRNA abundance was calculated compared with their respective untreated controls. Open and closed bars represent the cells grown in control conditions or in presence of the compounds. *p < 0.05, **p < 0.01, ***p < 0.001.
Article Snippet: The TaqMan assays used for the PCR were Glut2 (assay
Techniques: Quantitative RT-PCR, Control