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Image Search Results
Journal: International Journal of Biological Sciences
Article Title: DHCR24 Drives Ovarian Cancer Chemoresistance Through Lipid Raft-mediated P-gp Stabilization and STAT3 Activation
doi: 10.7150/ijbs.128173
Figure Lengend Snippet: DHCR24 regulates P-gp protein stability via cholesterol-rich lipid rafts in ovarian cancer cells. (A) Immunohistochemical staining of ovarian cancer tissues showing co-elevated expression of DHCR24 and P-gp. (B) Western blot analysis of P-gp protein expression in A2780/DDP and SKOV3/DDP cells after DHCR24 knockdown. (C) Quantitative PCR analysis of P-gp mRNA levels in DHCR24-silenced A2780/DDP and SKOV3/DDP cells. (D) Cycloheximide (CHX, 50 μ g) chase assay evaluating P-gp protein stability upon DHCR24 knockdown in A2780/DDP and SKOV3/DDP cells. (E) Western blot analysis of P-gp expression following lipid raft disruption via MβCD-mediated cholesterol depletion. (F) CHX chase assay assessing P-gp protein stability after MβCD treatment. (G) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after DHCR24 silencing. (H) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after MβCD treatment. (I) Immunohistochemical staining of P-gp in xenograft tumor tissues from the shDHCR24 and shctrl groups (same cohort as Fig. B). (J) Western blot analysis of P-gp protein levels in xenograft tumor tissues from the shDHCR24 and shctrl groups.
Article Snippet:
Techniques: Immunohistochemical staining, Staining, Expressing, Western Blot, Knockdown, Real-time Polymerase Chain Reaction, Disruption, Immunofluorescence