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sk br  (ATCC)
97
ATCC sk br
Sk Br, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories immpact dab substrate
Immpact Dab Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech antidkk1
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ATCC human cancer cells
Human Cancer Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC sk mel 28 cells
Sk Mel 28 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC human eoc cell lines skov3
DHCR24 regulates P-gp protein stability via cholesterol-rich lipid rafts in ovarian cancer cells. (A) Immunohistochemical staining of ovarian cancer tissues showing co-elevated expression of DHCR24 and P-gp. (B) Western blot analysis of P-gp protein expression in A2780/DDP and <t>SKOV3/DDP</t> cells after DHCR24 knockdown. (C) Quantitative PCR analysis of P-gp mRNA levels in DHCR24-silenced A2780/DDP and SKOV3/DDP cells. (D) Cycloheximide (CHX, 50 μ g) chase assay evaluating P-gp protein stability upon DHCR24 knockdown in A2780/DDP and SKOV3/DDP cells. (E) Western blot analysis of P-gp expression following lipid raft disruption via MβCD-mediated cholesterol depletion. (F) CHX chase assay assessing P-gp protein stability after MβCD treatment. (G) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after DHCR24 silencing. (H) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after MβCD treatment. (I) Immunohistochemical staining of P-gp in xenograft tumor tissues from the shDHCR24 and shctrl groups (same cohort as Fig. B). (J) Western blot analysis of P-gp protein levels in xenograft tumor tissues from the shDHCR24 and shctrl groups.
Human Eoc Cell Lines Skov3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories red alkaline phosphatase substrate
DHCR24 regulates P-gp protein stability via cholesterol-rich lipid rafts in ovarian cancer cells. (A) Immunohistochemical staining of ovarian cancer tissues showing co-elevated expression of DHCR24 and P-gp. (B) Western blot analysis of P-gp protein expression in A2780/DDP and <t>SKOV3/DDP</t> cells after DHCR24 knockdown. (C) Quantitative PCR analysis of P-gp mRNA levels in DHCR24-silenced A2780/DDP and SKOV3/DDP cells. (D) Cycloheximide (CHX, 50 μ g) chase assay evaluating P-gp protein stability upon DHCR24 knockdown in A2780/DDP and SKOV3/DDP cells. (E) Western blot analysis of P-gp expression following lipid raft disruption via MβCD-mediated cholesterol depletion. (F) CHX chase assay assessing P-gp protein stability after MβCD treatment. (G) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after DHCR24 silencing. (H) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after MβCD treatment. (I) Immunohistochemical staining of P-gp in xenograft tumor tissues from the shDHCR24 and shctrl groups (same cohort as Fig. B). (J) Western blot analysis of P-gp protein levels in xenograft tumor tissues from the shDHCR24 and shctrl groups.
Red Alkaline Phosphatase Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories dab kit
DHCR24 regulates P-gp protein stability via cholesterol-rich lipid rafts in ovarian cancer cells. (A) Immunohistochemical staining of ovarian cancer tissues showing co-elevated expression of DHCR24 and P-gp. (B) Western blot analysis of P-gp protein expression in A2780/DDP and <t>SKOV3/DDP</t> cells after DHCR24 knockdown. (C) Quantitative PCR analysis of P-gp mRNA levels in DHCR24-silenced A2780/DDP and SKOV3/DDP cells. (D) Cycloheximide (CHX, 50 μ g) chase assay evaluating P-gp protein stability upon DHCR24 knockdown in A2780/DDP and SKOV3/DDP cells. (E) Western blot analysis of P-gp expression following lipid raft disruption via MβCD-mediated cholesterol depletion. (F) CHX chase assay assessing P-gp protein stability after MβCD treatment. (G) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after DHCR24 silencing. (H) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after MβCD treatment. (I) Immunohistochemical staining of P-gp in xenograft tumor tissues from the shDHCR24 and shctrl groups (same cohort as Fig. B). (J) Western blot analysis of P-gp protein levels in xenograft tumor tissues from the shDHCR24 and shctrl groups.
Dab Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC sk br 3 tumor cells
DHCR24 regulates P-gp protein stability via cholesterol-rich lipid rafts in ovarian cancer cells. (A) Immunohistochemical staining of ovarian cancer tissues showing co-elevated expression of DHCR24 and P-gp. (B) Western blot analysis of P-gp protein expression in A2780/DDP and <t>SKOV3/DDP</t> cells after DHCR24 knockdown. (C) Quantitative PCR analysis of P-gp mRNA levels in DHCR24-silenced A2780/DDP and SKOV3/DDP cells. (D) Cycloheximide (CHX, 50 μ g) chase assay evaluating P-gp protein stability upon DHCR24 knockdown in A2780/DDP and SKOV3/DDP cells. (E) Western blot analysis of P-gp expression following lipid raft disruption via MβCD-mediated cholesterol depletion. (F) CHX chase assay assessing P-gp protein stability after MβCD treatment. (G) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after DHCR24 silencing. (H) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after MβCD treatment. (I) Immunohistochemical staining of P-gp in xenograft tumor tissues from the shDHCR24 and shctrl groups (same cohort as Fig. B). (J) Western blot analysis of P-gp protein levels in xenograft tumor tissues from the shDHCR24 and shctrl groups.
Sk Br 3 Tumor Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DSMZ sk br 3
DHCR24 regulates P-gp protein stability via cholesterol-rich lipid rafts in ovarian cancer cells. (A) Immunohistochemical staining of ovarian cancer tissues showing co-elevated expression of DHCR24 and P-gp. (B) Western blot analysis of P-gp protein expression in A2780/DDP and <t>SKOV3/DDP</t> cells after DHCR24 knockdown. (C) Quantitative PCR analysis of P-gp mRNA levels in DHCR24-silenced A2780/DDP and SKOV3/DDP cells. (D) Cycloheximide (CHX, 50 μ g) chase assay evaluating P-gp protein stability upon DHCR24 knockdown in A2780/DDP and SKOV3/DDP cells. (E) Western blot analysis of P-gp expression following lipid raft disruption via MβCD-mediated cholesterol depletion. (F) CHX chase assay assessing P-gp protein stability after MβCD treatment. (G) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after DHCR24 silencing. (H) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after MβCD treatment. (I) Immunohistochemical staining of P-gp in xenograft tumor tissues from the shDHCR24 and shctrl groups (same cohort as Fig. B). (J) Western blot analysis of P-gp protein levels in xenograft tumor tissues from the shDHCR24 and shctrl groups.
Sk Br 3, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Invent Biotechnologies bone tissue
DHCR24 regulates P-gp protein stability via cholesterol-rich lipid rafts in ovarian cancer cells. (A) Immunohistochemical staining of ovarian cancer tissues showing co-elevated expression of DHCR24 and P-gp. (B) Western blot analysis of P-gp protein expression in A2780/DDP and <t>SKOV3/DDP</t> cells after DHCR24 knockdown. (C) Quantitative PCR analysis of P-gp mRNA levels in DHCR24-silenced A2780/DDP and SKOV3/DDP cells. (D) Cycloheximide (CHX, 50 μ g) chase assay evaluating P-gp protein stability upon DHCR24 knockdown in A2780/DDP and SKOV3/DDP cells. (E) Western blot analysis of P-gp expression following lipid raft disruption via MβCD-mediated cholesterol depletion. (F) CHX chase assay assessing P-gp protein stability after MβCD treatment. (G) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after DHCR24 silencing. (H) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after MβCD treatment. (I) Immunohistochemical staining of P-gp in xenograft tumor tissues from the shDHCR24 and shctrl groups (same cohort as Fig. B). (J) Western blot analysis of P-gp protein levels in xenograft tumor tissues from the shDHCR24 and shctrl groups.
Bone Tissue, supplied by Invent Biotechnologies, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC sk mel 2
DHCR24 regulates P-gp protein stability via cholesterol-rich lipid rafts in ovarian cancer cells. (A) Immunohistochemical staining of ovarian cancer tissues showing co-elevated expression of DHCR24 and P-gp. (B) Western blot analysis of P-gp protein expression in A2780/DDP and <t>SKOV3/DDP</t> cells after DHCR24 knockdown. (C) Quantitative PCR analysis of P-gp mRNA levels in DHCR24-silenced A2780/DDP and SKOV3/DDP cells. (D) Cycloheximide (CHX, 50 μ g) chase assay evaluating P-gp protein stability upon DHCR24 knockdown in A2780/DDP and SKOV3/DDP cells. (E) Western blot analysis of P-gp expression following lipid raft disruption via MβCD-mediated cholesterol depletion. (F) CHX chase assay assessing P-gp protein stability after MβCD treatment. (G) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after DHCR24 silencing. (H) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after MβCD treatment. (I) Immunohistochemical staining of P-gp in xenograft tumor tissues from the shDHCR24 and shctrl groups (same cohort as Fig. B). (J) Western blot analysis of P-gp protein levels in xenograft tumor tissues from the shDHCR24 and shctrl groups.
Sk Mel 2, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


DHCR24 regulates P-gp protein stability via cholesterol-rich lipid rafts in ovarian cancer cells. (A) Immunohistochemical staining of ovarian cancer tissues showing co-elevated expression of DHCR24 and P-gp. (B) Western blot analysis of P-gp protein expression in A2780/DDP and SKOV3/DDP cells after DHCR24 knockdown. (C) Quantitative PCR analysis of P-gp mRNA levels in DHCR24-silenced A2780/DDP and SKOV3/DDP cells. (D) Cycloheximide (CHX, 50 μ g) chase assay evaluating P-gp protein stability upon DHCR24 knockdown in A2780/DDP and SKOV3/DDP cells. (E) Western blot analysis of P-gp expression following lipid raft disruption via MβCD-mediated cholesterol depletion. (F) CHX chase assay assessing P-gp protein stability after MβCD treatment. (G) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after DHCR24 silencing. (H) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after MβCD treatment. (I) Immunohistochemical staining of P-gp in xenograft tumor tissues from the shDHCR24 and shctrl groups (same cohort as Fig. B). (J) Western blot analysis of P-gp protein levels in xenograft tumor tissues from the shDHCR24 and shctrl groups.

Journal: International Journal of Biological Sciences

Article Title: DHCR24 Drives Ovarian Cancer Chemoresistance Through Lipid Raft-mediated P-gp Stabilization and STAT3 Activation

doi: 10.7150/ijbs.128173

Figure Lengend Snippet: DHCR24 regulates P-gp protein stability via cholesterol-rich lipid rafts in ovarian cancer cells. (A) Immunohistochemical staining of ovarian cancer tissues showing co-elevated expression of DHCR24 and P-gp. (B) Western blot analysis of P-gp protein expression in A2780/DDP and SKOV3/DDP cells after DHCR24 knockdown. (C) Quantitative PCR analysis of P-gp mRNA levels in DHCR24-silenced A2780/DDP and SKOV3/DDP cells. (D) Cycloheximide (CHX, 50 μ g) chase assay evaluating P-gp protein stability upon DHCR24 knockdown in A2780/DDP and SKOV3/DDP cells. (E) Western blot analysis of P-gp expression following lipid raft disruption via MβCD-mediated cholesterol depletion. (F) CHX chase assay assessing P-gp protein stability after MβCD treatment. (G) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after DHCR24 silencing. (H) Immunofluorescence staining of P-gp (green) and nuclei (DAPI, blue) in A2780/DDP cells after MβCD treatment. (I) Immunohistochemical staining of P-gp in xenograft tumor tissues from the shDHCR24 and shctrl groups (same cohort as Fig. B). (J) Western blot analysis of P-gp protein levels in xenograft tumor tissues from the shDHCR24 and shctrl groups.

Article Snippet: Human EOC cell lines SKOV3 and A2780 were obtained from ATCC (American Type Culture Collection), while their drug-resistant counterparts, SKOV3/DDP and A2780/DDP, were acquired from Wuhan Procell Life Science and Technology Co., Ltd. (Wuhan, China).

Techniques: Immunohistochemical staining, Staining, Expressing, Western Blot, Knockdown, Real-time Polymerase Chain Reaction, Disruption, Immunofluorescence