Journal: F1000Research

Article Title: Exploring the moderating effects of SIRT1 and gene polymorphisms rs7895833 on the relationship between hemoglobin levels and physical frailty in elderly adults with comorbid chronic diseases: A moderated mediation analysis

doi: 10.12688/f1000research.133517.3

Figure Lengend Snippet: Figure 1. SIRT1 SNP rs7895833 A>G representative PCR gel electrophoresis images.

Article Snippet: The plasma levels of SIRT1 was determined by a monoclonal antibody-based ELISA method using a commercially available human SIRT1 ELISA kit (Elabscience®, Houston, USA, E-EL-H1546).

Techniques: Nucleic Acid Electrophoresis

Journal: F1000Research

Article Title: Exploring the moderating effects of SIRT1 and gene polymorphisms rs7895833 on the relationship between hemoglobin levels and physical frailty in elderly adults with comorbid chronic diseases: A moderated mediation analysis

doi: 10.12688/f1000research.133517.3

Figure Lengend Snippet: Figure 2. The correlation plot of the interrelationship between the potential main variables. Hb (Hemoglobin); SIRT1 (Sirtuin1); Frail score (frailty); genotype (AA, AG-GG). Spearman's rank correlation with two tail significant: *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: The plasma levels of SIRT1 was determined by a monoclonal antibody-based ELISA method using a commercially available human SIRT1 ELISA kit (Elabscience®, Houston, USA, E-EL-H1546).

Techniques:

Journal: Oxidative medicine and cellular longevity

Article Title: SIRT1 Activity in Peripheral Blood Mononuclear Cells Correlates with Altered Lung Function in Patients with Chronic Obstructive Pulmonary Disease.

doi: 10.1155/2018/9391261

Figure Lengend Snippet: Figure 3: Linear regression analysis between Sirtuin 1 (SIRT1) activity in peripheral blood mononuclear cells (PBMCs) and forced expiratory volume in 1 second (FEV1) values (a); between SIRT1 activity in peripheral blood mononuclear cells (PBMCs) and forced expiratory volume in 1 second/forced vital capacity (FEV1/FVC) ratio values (b); and between SIRT1 activity in peripheral blood mononuclear cells (PBMCs) and serum TEAC (c). SIRT1 activity was determined in the nuclei extracted from PBMCs of healthy nonsmokers (HnS), healthy smokers (HS), and COPD patients (COPD). The multivariate analysis was also adjusted for age, gender, BMI, smoking pack/years, comorbidity, and drugs.

Article Snippet: SIRT1 expression was measured by enzyme-linked immunosorbent assay (SIRT1 ELISA Kit, Elabscience), following the manufacturer’s instructions.

Techniques: Activity Assay

Journal: Journal of Clinical Investigation

Article Title: The ZIP8/SIRT1 axis regulates alveolar progenitor cell renewal in aging and idiopathic pulmonary fibrosis

doi: 10.1172/jci157338

Figure Lengend Snippet: Figure 3. ZIP8-dependent zinc metabolism regulates AEC2 renewal through SIRT1. (A) IPA pathway analysis of AEC2s from healthy and IPF lungs.(B) Sir- tuin activation score of healthy and IPF AEC2s. (C and D) SIRT1 expression in freshly isolated AEC2s (C) and AEC2s derived from 3D organoids (D) by qPCR (n = 4–6, *P < 0.05). (E) SIRT1 expression of AEC2s derived from 3D-cultured organoids of ZIP8+ and ZIP8– AEC2s (n = 5 each, *P < 0.05). (F) Intracellular SIRT1 levels in healthy and IPF AEC2s without and with ZnSO4 treatment by flow cytometry (n = 3–4). (G) Intracellular SIRT1 levels in gated ZIP8+ and ZIP8– AEC2s from healthy lungs (n = 4). (H and I) CFE with 3D organoid culture of AEC2s from healthy lungs treated with either 1 μM SRT1720 (n = 4–5, ***P < 0.001 by ANOVA) (H) or 125 and 200 μM splitomicin (n = 3, ***P < 0.001, ****P < 0.0001 by ANOVA) (I). (J) CFE of AEC2s from IPF lungs cultured with SRT1720 at the indicated concentrations (n = 3, **P < 0.01, ****P < 0.0001 by ANOVA). (K) CFE of AEC2s from IPF lungs cultured with 1 μM SRT1720, 100 μM ZnSO4, or both (n = 3–4, ****P < 0.0001 by ANOVA). (L–N) A549 cells with SIRT1 knockout and control cells. SIRT1 ko 1, set 1 sgRNA; SIRT ko 2, set 2 sgRNA. (L) SIRT1 expression by qPCR. (M) SIRT1 expression by Western blot analysis; the same experiments were performed 3 times. (N) CFE with 3D organoid culture (n = 4, ***P < 0.001 by ANOVA). Data are shown as mean ± SEM. Unpaired 2-tailed Student’s t test.

Article Snippet: Human SIRT1 monoclonal mouse IgG1 (clone 834918, catalog IC7714S) from R&D Systems was used for intracellular staining.

Techniques: Activation Assay, Expressing, Isolation, Derivative Assay, Cell Culture, Flow Cytometry, Knock-Out, Control, Western Blot

Journal: Journal of Clinical Investigation

Article Title: The ZIP8/SIRT1 axis regulates alveolar progenitor cell renewal in aging and idiopathic pulmonary fibrosis

doi: 10.1172/jci157338

Figure Lengend Snippet: Figure 4. Downregulated ZIP8/SIRT1 signaling and decreased renewal capacity of AEC2s from old mouse lungs. (A) Flow cytometry analysis to gate out total AEC2s (R2), and ZIP8 expression AEC2s (R3) from lung homologies of young and old mice. (B) Percentage of ZIP8+ cells (R3) within total AEC2s (n = 5–6, **P < 0.01). (C) Number of ZIP8+ cells recovered from young and old mouse lung (n = 5–6, **P < 0.01). (D) CFE of mouse AEC2s isolated from young and old mouse lungs (n = 6–7, **P < 0.01). (E and F) Flow cytometry analysis of ZIP8 expression in gated AEC2s cultured with medium only or medium containing 100 μM ZnSO4 (n = 6, ***P < 0.001). (G–J) 3D organoid culture of AEC2s isolated from lungs of 2.5-, 12-, 14-, and 18-month-old mice with and without 100 μM ZnSO4 treatment. (G) CFE (n = 3–4, *P < 0.05, ***P < 0.001, ****P < 0.0001 by ANOVA). (H–J) Expression of Slc39a8 (H), Sftpc (I), and Pdpn (J) in AEC2s derived from 3D-cultured organoids with and without ZnSO4 treatment by qPCR (n = 3–4, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by ANOVA). (K) Violin plots of gene expression in AEC2s from lungs of bleomycin-treated young and old mice. (L) IPA pathway analysis of AEC2s from young and old mice on day 4 after bleomycin injury. (M and N) CFE of AEC2s from uninjured 10- to 12-week-old young mice treated with SRT1720 (n = 3–6, **P < 0.01, ****P < 0.0001 by ANOVA) (M) and splitomicin (n = 5–6, ****P < 0.0001 by ANOVA) (N) at the indicated doses and DMSO control. (O) CFE of AEC2s from uninjured 20- to 24-month-old mice treated with SRT1720 at the indicated doses and DMSO control (n = 4, **P < 0.01, ****P < 0.0001 by ANOVA). Data are shown as mean ± SEM. Unpaired 2-tailed Student’s t test.

Article Snippet: Human SIRT1 monoclonal mouse IgG1 (clone 834918, catalog IC7714S) from R&D Systems was used for intracellular staining.

Techniques: Flow Cytometry, Expressing, Isolation, Cell Culture, Derivative Assay, Gene Expression, Control

Journal: Journal of Clinical Investigation

Article Title: The ZIP8/SIRT1 axis regulates alveolar progenitor cell renewal in aging and idiopathic pulmonary fibrosis

doi: 10.1172/jci157338

Figure Lengend Snippet: Figure 5. Targeted deletion of Slc39a8 decreased AEC2 renewal. (A) ZIP-expressing cells among gated AEC2s and (B) percentage of ZIP8+ cells within the total AEC2 population from uninjured Zip8AEC2 and control mice by flow cytometry (n = 8, ****P < 0.001). (C and D) Intracellular zinc levels of AEC2s (C) and percentage of zinc+ AEC2s within the total AEC2 population (D) from Zip8AEC2 (n = 4) and control mice (n = 8) by flow cytometry (**P < 0.01). (E) CFE of flow-sorted AEC2s from uninjured Zip8AEC2 and control mice with 3D organoid culture (n = 6 each, *P < 0.05). (F and G) Expression of Sirt1 (n = 4, *P < 0.05) (F) and Pdpn (n = 5, **P < 0.01) (G) in AEC2s derived from 3D-cultured organoids. (H–J) AEC2s from day 4 bleomycin-injured Zip8AEC2 and control mice. (H) Number of AEC2s recovered per lung (n = 5, ***P < 0.001). (I and J) CFE of AEC2s with 3D organoid culture (n = 5–7, **P < 0.01) (I) and colony size (n = 28–86, ****P < 0.0001) (J). (K and L) Ki-67 expression by flow cytometry (n = 6 each, *P < 0.05) (K) and Pdpn expression by qPCR (n = 5 each, ****P < 0.0001) (L) in AEC2s derived from 3D-cultured organoids. (M and N) Violin plots of gene expression in AEC2s with scRNA-Seq. (M) AEC2s from 2-month-old (Young) and 18- to 20-month-old (Old) C57BL/6 WT mice (n = 3). (N) AEC2s from 10- to 12-week-old (young) Zip8AEC2 mice and littermate controls 2 weeks after 4 doses of tamoxifen injection. Data are shown as mean ± SEM. Unpaired 2-tailed Student’s t test.

Article Snippet: Human SIRT1 monoclonal mouse IgG1 (clone 834918, catalog IC7714S) from R&D Systems was used for intracellular staining.

Techniques: Expressing, Control, Flow Cytometry, Derivative Assay, Cell Culture, Gene Expression, Injection

Journal: Journal of Clinical Investigation

Article Title: The ZIP8/SIRT1 axis regulates alveolar progenitor cell renewal in aging and idiopathic pulmonary fibrosis

doi: 10.1172/jci157338

Figure Lengend Snippet: Figure 7. Summary of the role of the ZIP8/SIRT1 axis in regulating alveolar progenitor cell renewal. In young and healthy AEC2s, sufficient ZIP8 ensures adequate levels of intracellular zinc, SIRT1 activity, and AEC2 renewal capacity. However, in old AEC2s and IPF AEC2s, severely downregulated ZIP8 results in intracellular zinc deficiency and defective SIRT1 activity, which impairs AEC2 renewal. In addition, enzymes regulating NAD+ synthesis were downregulated in IPF AEC2s, further exaggerating SIRT1 impairment. Therefore, the optimal combinations of zinc, NAD+, and SIRT1 activation may restore AEC2 integrity and mitigate fibrosis. MT, metallothionein.

Article Snippet: Human SIRT1 monoclonal mouse IgG1 (clone 834918, catalog IC7714S) from R&D Systems was used for intracellular staining.

Techniques: Activity Assay, Activation Assay

Journal: Metabolic Brain Disease

Article Title: The SIRT-1/Nrf-2/HO-1 antioxidant defense axis in adult attention-deficit/hyperactivity disorder

doi: 10.1007/s11011-026-01845-5

Figure Lengend Snippet: ROC Curve Analysis for HO-1, Nrf-2 and SIRT-1. HO-1 (cut-off value 0.87, sensitivity 78.3%, specificity 76.7%); Nrf-2 (cut-off value 9.8 sensitivity 70%, specificity 73.3%); SIRT-1 (cut-off value 8.3, sensitivity 75%, specificity 70%). Abbreviations: HO-1: heme oxygenases-1; Nrf-2: nuclear factor erythroid 2-related factor 2; SIRT-1: Sirtuin 1

Article Snippet: Serum HO-1, NRF-2, and SIRT-1 concentrations were analyzed using ELISA kits according to the manufacturers’ standard protocols (BT Lab, Human Heme Oxygenase-1: Cat. No. E0932Hu; BT Lab, Human Nuclear Factor Erythroid 2-Related Factor 2: Cat. No. E3244Hu; BT Lab, Human Sirtuin-1: Cat. No. E2557Hu; Jiaxing Korain Biotech, Jiaxing, China) on a Rel Assay automated ELISA reader (Biobase Biodusty Co., Ltd., Jinan, China).

Techniques:

Journal: Metabolic Brain Disease

Article Title: The SIRT-1/Nrf-2/HO-1 antioxidant defense axis in adult attention-deficit/hyperactivity disorder

doi: 10.1007/s11011-026-01845-5

Figure Lengend Snippet: ROC Curve Analysis for HO-1, Nrf-2 and SIRT-1. HO-1 (cut-off value 0.87, sensitivity 78.3%, specificity 76.7%); Nrf-2 (cut-off value 9.8 sensitivity 70%, specificity 73.3%); SIRT-1 (cut-off value 8.3, sensitivity 75%, specificity 70%). Abbreviations: HO-1: heme oxygenases-1; Nrf-2: nuclear factor erythroid 2-related factor 2; SIRT-1: Sirtuin 1

Article Snippet: Serum HO-1, NRF-2, and SIRT-1 concentrations were analyzed using ELISA kits according to the manufacturers’ standard protocols (BT Lab, Human Heme Oxygenase-1: Cat. No. E0932Hu; BT Lab, Human Nuclear Factor Erythroid 2-Related Factor 2: Cat. No. E3244Hu; BT Lab, Human Sirtuin-1: Cat. No. E2557Hu; Jiaxing Korain Biotech, Jiaxing, China) on a Rel Assay automated ELISA reader (Biobase Biodusty Co., Ltd., Jinan, China).

Techniques: