singleplex Thermo Fisher Search Results


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  • 99
    Thermo Fisher quantigene singleplex assay kit
    Validation of <t>QuantiGene</t> probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse
    Quantigene Singleplex Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher singleplex bead kit
    Validation of <t>QuantiGene</t> probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse
    Singleplex Bead Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher singleplex taqman assays
    Validation of <t>QuantiGene</t> probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse
    Singleplex Taqman Assays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher cytokine singleplex assays
    Validation of <t>QuantiGene</t> probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse
    Cytokine Singleplex Assays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher il 13 human singleplex kit
    Validation of <t>QuantiGene</t> probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse
    Il 13 Human Singleplex Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher il 1 beta human singleplex kit
    Validation of <t>QuantiGene</t> probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse
    Il 1 Beta Human Singleplex Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher singleplex qpcr
    Validation of <t>QuantiGene</t> probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse
    Singleplex Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher il 6 monkey singleplex kit
    Validation of <t>QuantiGene</t> probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse
    Il 6 Monkey Singleplex Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher il8 human singleplex bead kit
    Validation of <t>QuantiGene</t> probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse
    Il8 Human Singleplex Bead Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher singleplex sige detection
    Validation of <t>QuantiGene</t> probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse
    Singleplex Sige Detection, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher singleplex luminometry
    Validation of <t>QuantiGene</t> probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse
    Singleplex Luminometry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher immunocap singleplex tests
    Validation of <t>QuantiGene</t> probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse
    Immunocap Singleplex Tests, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher mature mirna singleplex
    NF2 is downregulated by miR-92a-3p via its 3′UTR. (A) Schematic representation of the full and cloned 3′UTR region of NF2 isoform I mRNA and the predicted miR-92a-3p MREs within its sequence. Dual-Luciferase assay of (B) HCT116 and (C) A549 cells transfected with empty vector (i.e., pmirGLO) or NF2 −3′UTR-wild-type. (D and E) The expression levels of mature miR-92a-3p were measured by <t>QuantiGene</t> <t>miRNA</t> assay in untransfected (UTC) or miR-92a construct-transfected (OE) HCT116 and A549 cells. Dual-Luciferase assay of (F) HCT116 and (G) A549 cells co-transfected with increasing plasmid ratios of NF2 −3′UTR-wild-type:miR-92a. (H) Secondary structure analysis of the RNA-RNA interaction (boxed region) between miR-92a-3p (red) and its MRE within wild-type vs. mutant NF2 −3′UTR (green). Dual-Luciferase assay of (I) HCT116 and (J) A549 cells co-transfected with wild-type or miR-92a-3p MRE mutant NF2 −3′UTR and miR-92a expression vectors. All experiments were performed in cells maintained in 0.5% serum. Data presented are representative of three independent trials and expressed as the mean ± standard deviation (SD). *P≤0.05, **P≤0.01, ***P≤0.001. NF2 , neurofibromin 2; UTR, untranslated region; MRE, microRNA response element.
    Mature Mirna Singleplex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2725 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher ultrasensitive singleplex bead kit
    NF2 is downregulated by miR-92a-3p via its 3′UTR. (A) Schematic representation of the full and cloned 3′UTR region of NF2 isoform I mRNA and the predicted miR-92a-3p MREs within its sequence. Dual-Luciferase assay of (B) HCT116 and (C) A549 cells transfected with empty vector (i.e., pmirGLO) or NF2 −3′UTR-wild-type. (D and E) The expression levels of mature miR-92a-3p were measured by <t>QuantiGene</t> <t>miRNA</t> assay in untransfected (UTC) or miR-92a construct-transfected (OE) HCT116 and A549 cells. Dual-Luciferase assay of (F) HCT116 and (G) A549 cells co-transfected with increasing plasmid ratios of NF2 −3′UTR-wild-type:miR-92a. (H) Secondary structure analysis of the RNA-RNA interaction (boxed region) between miR-92a-3p (red) and its MRE within wild-type vs. mutant NF2 −3′UTR (green). Dual-Luciferase assay of (I) HCT116 and (J) A549 cells co-transfected with wild-type or miR-92a-3p MRE mutant NF2 −3′UTR and miR-92a expression vectors. All experiments were performed in cells maintained in 0.5% serum. Data presented are representative of three independent trials and expressed as the mean ± standard deviation (SD). *P≤0.05, **P≤0.01, ***P≤0.001. NF2 , neurofibromin 2; UTR, untranslated region; MRE, microRNA response element.
    Ultrasensitive Singleplex Bead Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher mouse procarta singleplex assay
    NF2 is downregulated by miR-92a-3p via its 3′UTR. (A) Schematic representation of the full and cloned 3′UTR region of NF2 isoform I mRNA and the predicted miR-92a-3p MREs within its sequence. Dual-Luciferase assay of (B) HCT116 and (C) A549 cells transfected with empty vector (i.e., pmirGLO) or NF2 −3′UTR-wild-type. (D and E) The expression levels of mature miR-92a-3p were measured by <t>QuantiGene</t> <t>miRNA</t> assay in untransfected (UTC) or miR-92a construct-transfected (OE) HCT116 and A549 cells. Dual-Luciferase assay of (F) HCT116 and (G) A549 cells co-transfected with increasing plasmid ratios of NF2 −3′UTR-wild-type:miR-92a. (H) Secondary structure analysis of the RNA-RNA interaction (boxed region) between miR-92a-3p (red) and its MRE within wild-type vs. mutant NF2 −3′UTR (green). Dual-Luciferase assay of (I) HCT116 and (J) A549 cells co-transfected with wild-type or miR-92a-3p MRE mutant NF2 −3′UTR and miR-92a expression vectors. All experiments were performed in cells maintained in 0.5% serum. Data presented are representative of three independent trials and expressed as the mean ± standard deviation (SD). *P≤0.05, **P≤0.01, ***P≤0.001. NF2 , neurofibromin 2; UTR, untranslated region; MRE, microRNA response element.
    Mouse Procarta Singleplex Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher singleplex analysis
    NF2 is downregulated by miR-92a-3p via its 3′UTR. (A) Schematic representation of the full and cloned 3′UTR region of NF2 isoform I mRNA and the predicted miR-92a-3p MREs within its sequence. Dual-Luciferase assay of (B) HCT116 and (C) A549 cells transfected with empty vector (i.e., pmirGLO) or NF2 −3′UTR-wild-type. (D and E) The expression levels of mature miR-92a-3p were measured by <t>QuantiGene</t> <t>miRNA</t> assay in untransfected (UTC) or miR-92a construct-transfected (OE) HCT116 and A549 cells. Dual-Luciferase assay of (F) HCT116 and (G) A549 cells co-transfected with increasing plasmid ratios of NF2 −3′UTR-wild-type:miR-92a. (H) Secondary structure analysis of the RNA-RNA interaction (boxed region) between miR-92a-3p (red) and its MRE within wild-type vs. mutant NF2 −3′UTR (green). Dual-Luciferase assay of (I) HCT116 and (J) A549 cells co-transfected with wild-type or miR-92a-3p MRE mutant NF2 −3′UTR and miR-92a expression vectors. All experiments were performed in cells maintained in 0.5% serum. Data presented are representative of three independent trials and expressed as the mean ± standard deviation (SD). *P≤0.05, **P≤0.01, ***P≤0.001. NF2 , neurofibromin 2; UTR, untranslated region; MRE, microRNA response element.
    Singleplex Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher il 6 human singleplex kit
    NF2 is downregulated by miR-92a-3p via its 3′UTR. (A) Schematic representation of the full and cloned 3′UTR region of NF2 isoform I mRNA and the predicted miR-92a-3p MREs within its sequence. Dual-Luciferase assay of (B) HCT116 and (C) A549 cells transfected with empty vector (i.e., pmirGLO) or NF2 −3′UTR-wild-type. (D and E) The expression levels of mature miR-92a-3p were measured by <t>QuantiGene</t> <t>miRNA</t> assay in untransfected (UTC) or miR-92a construct-transfected (OE) HCT116 and A549 cells. Dual-Luciferase assay of (F) HCT116 and (G) A549 cells co-transfected with increasing plasmid ratios of NF2 −3′UTR-wild-type:miR-92a. (H) Secondary structure analysis of the RNA-RNA interaction (boxed region) between miR-92a-3p (red) and its MRE within wild-type vs. mutant NF2 −3′UTR (green). Dual-Luciferase assay of (I) HCT116 and (J) A549 cells co-transfected with wild-type or miR-92a-3p MRE mutant NF2 −3′UTR and miR-92a expression vectors. All experiments were performed in cells maintained in 0.5% serum. Data presented are representative of three independent trials and expressed as the mean ± standard deviation (SD). *P≤0.05, **P≤0.01, ***P≤0.001. NF2 , neurofibromin 2; UTR, untranslated region; MRE, microRNA response element.
    Il 6 Human Singleplex Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher singleplex
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Singleplex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher singleplex fluorescent bead based array
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Singleplex Fluorescent Bead Based Array, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher singleplex bead immunoassay
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Singleplex Bead Immunoassay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher separate wells singleplex
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Separate Wells Singleplex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher singleplex control
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Singleplex Control, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher singleplex elisa
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Singleplex Elisa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher ige detection immunoassays
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Ige Detection Immunoassays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher maxima hot start pcr master mix
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Maxima Hot Start Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 385 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher singleplex taqman gene expression assays
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Singleplex Taqman Gene Expression Assays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher immunocap singleplex system
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Immunocap Singleplex System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher il 17 singleplex kit
    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based <t>singleplex</t> approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.
    Il 17 Singleplex Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher murine tnf α
    Mice lacking Rab27a but not Munc13-4 have impaired in vivo secretion of <t>TNF-α</t> in response to LPS. Munc13-4 jinx / jinx ( jinx ), Rab27a ash / ash ( ashen ), and wild-type (WT) mice were challenged with a single intraperitoneal injection of LPS or PBS. Blood
    Murine Tnf α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher poweruptm sybr green master mix
    Mice lacking Rab27a but not Munc13-4 have impaired in vivo secretion of <t>TNF-α</t> in response to LPS. Munc13-4 jinx / jinx ( jinx ), Rab27a ash / ash ( ashen ), and wild-type (WT) mice were challenged with a single intraperitoneal injection of LPS or PBS. Blood
    Poweruptm Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Thermo Fisher fgf basic singleplex bead kit
    Mice lacking Rab27a but not Munc13-4 have impaired in vivo secretion of <t>TNF-α</t> in response to LPS. Munc13-4 jinx / jinx ( jinx ), Rab27a ash / ash ( ashen ), and wild-type (WT) mice were challenged with a single intraperitoneal injection of LPS or PBS. Blood
    Fgf Basic Singleplex Bead Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Validation of QuantiGene probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse

    Journal: Nucleic Acid Therapeutics

    Article Title: A High-Throughput Method for Direct Detection of Therapeutic Oligonucleotide-Induced Gene Silencing In Vivo

    doi: 10.1089/nat.2015.0578

    Figure Lengend Snippet: Validation of QuantiGene probes sets shows good linearity with high signal to noise ratio for several mRNA targets in different tissues. Various mRNAs were quantified from different tissue lysates. (A) Human Huntingtin ( HTT ) quantified from YAC128 mouse

    Article Snippet: The following day, the remainder of the QuantiGene assay protocol was performed (#QS0011; Affymetrix).

    Techniques:

    Combination of TissueLyser II with QuantiGene ® bDNA: A high-throughput method of direct mRNA detection from tissues biopsies. Top panel: Punch biopsies from consecutively sectioned coronal brain samples are collected. Biopsies are incubated at

    Journal: Nucleic Acid Therapeutics

    Article Title: A High-Throughput Method for Direct Detection of Therapeutic Oligonucleotide-Induced Gene Silencing In Vivo

    doi: 10.1089/nat.2015.0578

    Figure Lengend Snippet: Combination of TissueLyser II with QuantiGene ® bDNA: A high-throughput method of direct mRNA detection from tissues biopsies. Top panel: Punch biopsies from consecutively sectioned coronal brain samples are collected. Biopsies are incubated at

    Article Snippet: The following day, the remainder of the QuantiGene assay protocol was performed (#QS0011; Affymetrix).

    Techniques: High Throughput Screening Assay, Incubation

    NF2 is downregulated by miR-92a-3p via its 3′UTR. (A) Schematic representation of the full and cloned 3′UTR region of NF2 isoform I mRNA and the predicted miR-92a-3p MREs within its sequence. Dual-Luciferase assay of (B) HCT116 and (C) A549 cells transfected with empty vector (i.e., pmirGLO) or NF2 −3′UTR-wild-type. (D and E) The expression levels of mature miR-92a-3p were measured by QuantiGene miRNA assay in untransfected (UTC) or miR-92a construct-transfected (OE) HCT116 and A549 cells. Dual-Luciferase assay of (F) HCT116 and (G) A549 cells co-transfected with increasing plasmid ratios of NF2 −3′UTR-wild-type:miR-92a. (H) Secondary structure analysis of the RNA-RNA interaction (boxed region) between miR-92a-3p (red) and its MRE within wild-type vs. mutant NF2 −3′UTR (green). Dual-Luciferase assay of (I) HCT116 and (J) A549 cells co-transfected with wild-type or miR-92a-3p MRE mutant NF2 −3′UTR and miR-92a expression vectors. All experiments were performed in cells maintained in 0.5% serum. Data presented are representative of three independent trials and expressed as the mean ± standard deviation (SD). *P≤0.05, **P≤0.01, ***P≤0.001. NF2 , neurofibromin 2; UTR, untranslated region; MRE, microRNA response element.

    Journal: Oncology Reports

    Article Title: MicroRNA-92a promotes cell proliferation, migration and survival by directly targeting the tumor suppressor gene NF2 in colorectal and lung cancer cells

    doi: 10.3892/or.2019.7020

    Figure Lengend Snippet: NF2 is downregulated by miR-92a-3p via its 3′UTR. (A) Schematic representation of the full and cloned 3′UTR region of NF2 isoform I mRNA and the predicted miR-92a-3p MREs within its sequence. Dual-Luciferase assay of (B) HCT116 and (C) A549 cells transfected with empty vector (i.e., pmirGLO) or NF2 −3′UTR-wild-type. (D and E) The expression levels of mature miR-92a-3p were measured by QuantiGene miRNA assay in untransfected (UTC) or miR-92a construct-transfected (OE) HCT116 and A549 cells. Dual-Luciferase assay of (F) HCT116 and (G) A549 cells co-transfected with increasing plasmid ratios of NF2 −3′UTR-wild-type:miR-92a. (H) Secondary structure analysis of the RNA-RNA interaction (boxed region) between miR-92a-3p (red) and its MRE within wild-type vs. mutant NF2 −3′UTR (green). Dual-Luciferase assay of (I) HCT116 and (J) A549 cells co-transfected with wild-type or miR-92a-3p MRE mutant NF2 −3′UTR and miR-92a expression vectors. All experiments were performed in cells maintained in 0.5% serum. Data presented are representative of three independent trials and expressed as the mean ± standard deviation (SD). *P≤0.05, **P≤0.01, ***P≤0.001. NF2 , neurofibromin 2; UTR, untranslated region; MRE, microRNA response element.

    Article Snippet: QuantiGene Singleplex miRNA probes were obtained from the pre-designed probes offered by eBioscience (Thermo Fisher Scientific, Inc.).

    Techniques: Clone Assay, Sequencing, Luciferase, Transfection, Plasmid Preparation, Expressing, Construct, Mutagenesis, Standard Deviation

    The shift from extract-based to molecule-based allergy diagnosis. For molecule-based singleplex approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.

    Journal: Yonsei Medical Journal

    Article Title: Molecular Approach to Allergy Diagnosis and Therapy

    doi: 10.3349/ymj.2014.55.4.839

    Figure Lengend Snippet: The shift from extract-based to molecule-based allergy diagnosis. For molecule-based singleplex approaches, the number of tests to be performed can be very high. Allergen microarrays offer the advantage of testing a large panel of molecules in one single test.

    Article Snippet: Presently, manufacturers offer molecular allergy diagnosis in singleplex (i.e., Thermo Fisher ImmunoCAP, Siemens ImmuLite, and HyCor HyTec) or multiplex [Thermo Fisher Immuno-Solid phase Allergen Chip (ISAC)] formats; both systems have their advantages and limitations.

    Techniques:

    Mice lacking Rab27a but not Munc13-4 have impaired in vivo secretion of TNF-α in response to LPS. Munc13-4 jinx / jinx ( jinx ), Rab27a ash / ash ( ashen ), and wild-type (WT) mice were challenged with a single intraperitoneal injection of LPS or PBS. Blood

    Journal: Infection and Immunity

    Article Title: Increased Survival and Reduced Neutrophil Infiltration of the Liver in Rab27a- but Not Munc13-4-Deficient Mice in Lipopolysaccharide-Induced Systemic Inflammation ▿

    doi: 10.1128/IAI.05043-11

    Figure Lengend Snippet: Mice lacking Rab27a but not Munc13-4 have impaired in vivo secretion of TNF-α in response to LPS. Munc13-4 jinx / jinx ( jinx ), Rab27a ash / ash ( ashen ), and wild-type (WT) mice were challenged with a single intraperitoneal injection of LPS or PBS. Blood

    Article Snippet: Plasma levels of tumor necrosis factor alpha (TNF-α) were determined using a singleplex assay specific for murine TNF-α (Invitrogen).

    Techniques: Mouse Assay, In Vivo, Injection