sigmoidal 4pl Search Results


sigmoid 4pl interpolation  (GraphPad Software Inc)
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GraphPad Software Inc sigmoid 4pl interpolation
The neutralization titers (50% inhibition) in immunized mice against SPΔC wt -Luc-PVs (A), SPΔCdelta-Luc-PVs (B), SPΔC beta’ -Luc-PVs (D) and SPΔC b.1.617 -Luc-PVs (E) after 28 days of prime-boost vaccination with different bivalent vaccines. VSV-G-Luc-PVPs (C) was used as negative control. The mouse serum of each immunization group collected at day 28 were pooled together, 2× serially diluted and incubated with different Luc-PVs (∼10 4 RLU). Then, the mixtures were added in A549 ACE2 . cell cultures and the infection of PVs was determined by Luciferase assay at 48∼66 hrs post infection. The percentage of infection was calculated compared with no serum control and neutralizing titers were calculated by using sigmoid <t>4PL</t> <t>interpolation</t> with GraphPad Prism 9.0, as described in Materials and Methods. The neutralizing titers of V-EM2/SPΔC1 immunized mouse sera against different SPΔC-Luc-PVs were listed (F). Data represent Mean ±SD and were obtained from over three independent experiments. Statistical significance was determined using ordinary one-way ANOVA test and Turkey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
Sigmoid 4pl Interpolation, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nonlinear regression (sigmoidal, 4pl) using graphpad prism 8  (GraphPad Software Inc)
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GraphPad Software Inc nonlinear regression (sigmoidal, 4pl) using graphpad prism 8
The neutralization titers (50% inhibition) in immunized mice against SPΔC wt -Luc-PVs (A), SPΔCdelta-Luc-PVs (B), SPΔC beta’ -Luc-PVs (D) and SPΔC b.1.617 -Luc-PVs (E) after 28 days of prime-boost vaccination with different bivalent vaccines. VSV-G-Luc-PVPs (C) was used as negative control. The mouse serum of each immunization group collected at day 28 were pooled together, 2× serially diluted and incubated with different Luc-PVs (∼10 4 RLU). Then, the mixtures were added in A549 ACE2 . cell cultures and the infection of PVs was determined by Luciferase assay at 48∼66 hrs post infection. The percentage of infection was calculated compared with no serum control and neutralizing titers were calculated by using sigmoid <t>4PL</t> <t>interpolation</t> with GraphPad Prism 9.0, as described in Materials and Methods. The neutralizing titers of V-EM2/SPΔC1 immunized mouse sera against different SPΔC-Luc-PVs were listed (F). Data represent Mean ±SD and were obtained from over three independent experiments. Statistical significance was determined using ordinary one-way ANOVA test and Turkey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
Nonlinear Regression (Sigmoidal, 4pl) Using Graphpad Prism 8, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sigmoidal 4pl, x is concentration  (GraphPad Software Inc)
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GraphPad Software Inc sigmoidal 4pl, x is concentration
The neutralization titers (50% inhibition) in immunized mice against SPΔC wt -Luc-PVs (A), SPΔCdelta-Luc-PVs (B), SPΔC beta’ -Luc-PVs (D) and SPΔC b.1.617 -Luc-PVs (E) after 28 days of prime-boost vaccination with different bivalent vaccines. VSV-G-Luc-PVPs (C) was used as negative control. The mouse serum of each immunization group collected at day 28 were pooled together, 2× serially diluted and incubated with different Luc-PVs (∼10 4 RLU). Then, the mixtures were added in A549 ACE2 . cell cultures and the infection of PVs was determined by Luciferase assay at 48∼66 hrs post infection. The percentage of infection was calculated compared with no serum control and neutralizing titers were calculated by using sigmoid <t>4PL</t> <t>interpolation</t> with GraphPad Prism 9.0, as described in Materials and Methods. The neutralizing titers of V-EM2/SPΔC1 immunized mouse sera against different SPΔC-Luc-PVs were listed (F). Data represent Mean ±SD and were obtained from over three independent experiments. Statistical significance was determined using ordinary one-way ANOVA test and Turkey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
Sigmoidal 4pl, X Is Concentration, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nonlinear regression curve fit (log (agonist) vs. response) in prism 6.0  (GraphPad Software Inc)
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GraphPad Software Inc nonlinear regression curve fit (log (agonist) vs. response) in prism 6.0
The neutralization titers (50% inhibition) in immunized mice against SPΔC wt -Luc-PVs (A), SPΔCdelta-Luc-PVs (B), SPΔC beta’ -Luc-PVs (D) and SPΔC b.1.617 -Luc-PVs (E) after 28 days of prime-boost vaccination with different bivalent vaccines. VSV-G-Luc-PVPs (C) was used as negative control. The mouse serum of each immunization group collected at day 28 were pooled together, 2× serially diluted and incubated with different Luc-PVs (∼10 4 RLU). Then, the mixtures were added in A549 ACE2 . cell cultures and the infection of PVs was determined by Luciferase assay at 48∼66 hrs post infection. The percentage of infection was calculated compared with no serum control and neutralizing titers were calculated by using sigmoid <t>4PL</t> <t>interpolation</t> with GraphPad Prism 9.0, as described in Materials and Methods. The neutralizing titers of V-EM2/SPΔC1 immunized mouse sera against different SPΔC-Luc-PVs were listed (F). Data represent Mean ±SD and were obtained from over three independent experiments. Statistical significance was determined using ordinary one-way ANOVA test and Turkey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
Nonlinear Regression Curve Fit (Log (Agonist) Vs. Response) In Prism 6.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sigmoidal, 4pl model  (GraphPad Software Inc)
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GraphPad Software Inc sigmoidal, 4pl model
The neutralization titers (50% inhibition) in immunized mice against SPΔC wt -Luc-PVs (A), SPΔCdelta-Luc-PVs (B), SPΔC beta’ -Luc-PVs (D) and SPΔC b.1.617 -Luc-PVs (E) after 28 days of prime-boost vaccination with different bivalent vaccines. VSV-G-Luc-PVPs (C) was used as negative control. The mouse serum of each immunization group collected at day 28 were pooled together, 2× serially diluted and incubated with different Luc-PVs (∼10 4 RLU). Then, the mixtures were added in A549 ACE2 . cell cultures and the infection of PVs was determined by Luciferase assay at 48∼66 hrs post infection. The percentage of infection was calculated compared with no serum control and neutralizing titers were calculated by using sigmoid <t>4PL</t> <t>interpolation</t> with GraphPad Prism 9.0, as described in Materials and Methods. The neutralizing titers of V-EM2/SPΔC1 immunized mouse sera against different SPΔC-Luc-PVs were listed (F). Data represent Mean ±SD and were obtained from over three independent experiments. Statistical significance was determined using ordinary one-way ANOVA test and Turkey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
Sigmoidal, 4pl Model, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sigmoidal 4pl curve fit in graphpad prism v 9.4 software  (GraphPad Software Inc)
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GraphPad Software Inc sigmoidal 4pl curve fit in graphpad prism v 9.4 software
The neutralization titers (50% inhibition) in immunized mice against SPΔC wt -Luc-PVs (A), SPΔCdelta-Luc-PVs (B), SPΔC beta’ -Luc-PVs (D) and SPΔC b.1.617 -Luc-PVs (E) after 28 days of prime-boost vaccination with different bivalent vaccines. VSV-G-Luc-PVPs (C) was used as negative control. The mouse serum of each immunization group collected at day 28 were pooled together, 2× serially diluted and incubated with different Luc-PVs (∼10 4 RLU). Then, the mixtures were added in A549 ACE2 . cell cultures and the infection of PVs was determined by Luciferase assay at 48∼66 hrs post infection. The percentage of infection was calculated compared with no serum control and neutralizing titers were calculated by using sigmoid <t>4PL</t> <t>interpolation</t> with GraphPad Prism 9.0, as described in Materials and Methods. The neutralizing titers of V-EM2/SPΔC1 immunized mouse sera against different SPΔC-Luc-PVs were listed (F). Data represent Mean ±SD and were obtained from over three independent experiments. Statistical significance was determined using ordinary one-way ANOVA test and Turkey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
Sigmoidal 4pl Curve Fit In Graphpad Prism V 9.4 Software, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sigmoidal 4 pl analysis with graphpad prism 8.2  (GraphPad Software Inc)
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GraphPad Software Inc sigmoidal 4 pl analysis with graphpad prism 8.2
The neutralization titers (50% inhibition) in immunized mice against SPΔC wt -Luc-PVs (A), SPΔCdelta-Luc-PVs (B), SPΔC beta’ -Luc-PVs (D) and SPΔC b.1.617 -Luc-PVs (E) after 28 days of prime-boost vaccination with different bivalent vaccines. VSV-G-Luc-PVPs (C) was used as negative control. The mouse serum of each immunization group collected at day 28 were pooled together, 2× serially diluted and incubated with different Luc-PVs (∼10 4 RLU). Then, the mixtures were added in A549 ACE2 . cell cultures and the infection of PVs was determined by Luciferase assay at 48∼66 hrs post infection. The percentage of infection was calculated compared with no serum control and neutralizing titers were calculated by using sigmoid <t>4PL</t> <t>interpolation</t> with GraphPad Prism 9.0, as described in Materials and Methods. The neutralizing titers of V-EM2/SPΔC1 immunized mouse sera against different SPΔC-Luc-PVs were listed (F). Data represent Mean ±SD and were obtained from over three independent experiments. Statistical significance was determined using ordinary one-way ANOVA test and Turkey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
Sigmoidal 4 Pl Analysis With Graphpad Prism 8.2, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sigmoidal 4pl fit  (GraphPad Software Inc)
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GraphPad Software Inc sigmoidal 4pl fit
The neutralization titers (50% inhibition) in immunized mice against SPΔC wt -Luc-PVs (A), SPΔCdelta-Luc-PVs (B), SPΔC beta’ -Luc-PVs (D) and SPΔC b.1.617 -Luc-PVs (E) after 28 days of prime-boost vaccination with different bivalent vaccines. VSV-G-Luc-PVPs (C) was used as negative control. The mouse serum of each immunization group collected at day 28 were pooled together, 2× serially diluted and incubated with different Luc-PVs (∼10 4 RLU). Then, the mixtures were added in A549 ACE2 . cell cultures and the infection of PVs was determined by Luciferase assay at 48∼66 hrs post infection. The percentage of infection was calculated compared with no serum control and neutralizing titers were calculated by using sigmoid <t>4PL</t> <t>interpolation</t> with GraphPad Prism 9.0, as described in Materials and Methods. The neutralizing titers of V-EM2/SPΔC1 immunized mouse sera against different SPΔC-Luc-PVs were listed (F). Data represent Mean ±SD and were obtained from over three independent experiments. Statistical significance was determined using ordinary one-way ANOVA test and Turkey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
Sigmoidal 4pl Fit, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prism 8 sigmoidal 4 pl model  (GraphPad Software Inc)
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The neutralization titers (50% inhibition) in immunized mice against SPΔC wt -Luc-PVs (A), SPΔCdelta-Luc-PVs (B), SPΔC beta’ -Luc-PVs (D) and SPΔC b.1.617 -Luc-PVs (E) after 28 days of prime-boost vaccination with different bivalent vaccines. VSV-G-Luc-PVPs (C) was used as negative control. The mouse serum of each immunization group collected at day 28 were pooled together, 2× serially diluted and incubated with different Luc-PVs (∼10 4 RLU). Then, the mixtures were added in A549 ACE2 . cell cultures and the infection of PVs was determined by Luciferase assay at 48∼66 hrs post infection. The percentage of infection was calculated compared with no serum control and neutralizing titers were calculated by using sigmoid <t>4PL</t> <t>interpolation</t> with GraphPad Prism 9.0, as described in Materials and Methods. The neutralizing titers of V-EM2/SPΔC1 immunized mouse sera against different SPΔC-Luc-PVs were listed (F). Data represent Mean ±SD and were obtained from over three independent experiments. Statistical significance was determined using ordinary one-way ANOVA test and Turkey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
Prism 8 Sigmoidal 4 Pl Model, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sigmoidal 4pl option  (GraphPad Software Inc)
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Comparison of the percentage of tumor cell staining for each programmed death ligand 1 (PD‐L1) assay (the 22C3, 28–8, SP263, and SP142 assays), by case, with/without alternating current (AC) mixing. (a) Conventional immunohistochemistry (IHC) ( ) 22C3, ( ) 28‐8, ( ) SP263, ( ) SP142. (b) AC mixing IHC ( ) 22C3, ( ) 28‐8 AC, ( ) SP263 AC, ( ) SP142 AC. The standard IHC is the 22C3 phamDx IHC on Autostainer Link 48 platform in both figures. Best fit colored curves enable comparison of score ranges between the four assays. When assessing PD‐L1 TPS in the cases of 22C3 PD‐L1 >1%‐positive patients using AC mixing, the nonlinear best fit curve calculated using the sigmoidal <t>4PL</t> method showed one curve for all four PD‐L1 antibodies (extra sum‐of squares F test, p = 0.2552)
Sigmoidal 4pl Option, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sigmoidal 4pl robust fit regression method  (GraphPad Software Inc)
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Comparison of the percentage of tumor cell staining for each programmed death ligand 1 (PD‐L1) assay (the 22C3, 28–8, SP263, and SP142 assays), by case, with/without alternating current (AC) mixing. (a) Conventional immunohistochemistry (IHC) ( ) 22C3, ( ) 28‐8, ( ) SP263, ( ) SP142. (b) AC mixing IHC ( ) 22C3, ( ) 28‐8 AC, ( ) SP263 AC, ( ) SP142 AC. The standard IHC is the 22C3 phamDx IHC on Autostainer Link 48 platform in both figures. Best fit colored curves enable comparison of score ranges between the four assays. When assessing PD‐L1 TPS in the cases of 22C3 PD‐L1 >1%‐positive patients using AC mixing, the nonlinear best fit curve calculated using the sigmoidal <t>4PL</t> method showed one curve for all four PD‐L1 antibodies (extra sum‐of squares F test, p = 0.2552)
Sigmoidal 4pl Robust Fit Regression Method, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sigmoid 4pl interpolation graphpad prism 9.0  (GraphPad Software Inc)
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GraphPad Software Inc sigmoid 4pl interpolation graphpad prism 9.0
A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.
Sigmoid 4pl Interpolation Graphpad Prism 9.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The neutralization titers (50% inhibition) in immunized mice against SPΔC wt -Luc-PVs (A), SPΔCdelta-Luc-PVs (B), SPΔC beta’ -Luc-PVs (D) and SPΔC b.1.617 -Luc-PVs (E) after 28 days of prime-boost vaccination with different bivalent vaccines. VSV-G-Luc-PVPs (C) was used as negative control. The mouse serum of each immunization group collected at day 28 were pooled together, 2× serially diluted and incubated with different Luc-PVs (∼10 4 RLU). Then, the mixtures were added in A549 ACE2 . cell cultures and the infection of PVs was determined by Luciferase assay at 48∼66 hrs post infection. The percentage of infection was calculated compared with no serum control and neutralizing titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. The neutralizing titers of V-EM2/SPΔC1 immunized mouse sera against different SPΔC-Luc-PVs were listed (F). Data represent Mean ±SD and were obtained from over three independent experiments. Statistical significance was determined using ordinary one-way ANOVA test and Turkey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

Journal: bioRxiv

Article Title: Development and Characterization of Recombinant Vesicular Stomatitis Virus (rVSV)-based Bivalent Vaccine Against COVID-19 Delta Variant and Influenza Virus

doi: 10.1101/2021.12.14.472657

Figure Lengend Snippet: The neutralization titers (50% inhibition) in immunized mice against SPΔC wt -Luc-PVs (A), SPΔCdelta-Luc-PVs (B), SPΔC beta’ -Luc-PVs (D) and SPΔC b.1.617 -Luc-PVs (E) after 28 days of prime-boost vaccination with different bivalent vaccines. VSV-G-Luc-PVPs (C) was used as negative control. The mouse serum of each immunization group collected at day 28 were pooled together, 2× serially diluted and incubated with different Luc-PVs (∼10 4 RLU). Then, the mixtures were added in A549 ACE2 . cell cultures and the infection of PVs was determined by Luciferase assay at 48∼66 hrs post infection. The percentage of infection was calculated compared with no serum control and neutralizing titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. The neutralizing titers of V-EM2/SPΔC1 immunized mouse sera against different SPΔC-Luc-PVs were listed (F). Data represent Mean ±SD and were obtained from over three independent experiments. Statistical significance was determined using ordinary one-way ANOVA test and Turkey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

Article Snippet: The endpoint titer is designated as the reciprocal of the highest dilution of a serum that has an OD450 above the cutoff (10× negative control) and is calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0.

Techniques: Neutralization, Inhibition, Vaccines, Negative Control, Incubation, Infection, Luciferase, Control

Comparison of the percentage of tumor cell staining for each programmed death ligand 1 (PD‐L1) assay (the 22C3, 28–8, SP263, and SP142 assays), by case, with/without alternating current (AC) mixing. (a) Conventional immunohistochemistry (IHC) ( ) 22C3, ( ) 28‐8, ( ) SP263, ( ) SP142. (b) AC mixing IHC ( ) 22C3, ( ) 28‐8 AC, ( ) SP263 AC, ( ) SP142 AC. The standard IHC is the 22C3 phamDx IHC on Autostainer Link 48 platform in both figures. Best fit colored curves enable comparison of score ranges between the four assays. When assessing PD‐L1 TPS in the cases of 22C3 PD‐L1 >1%‐positive patients using AC mixing, the nonlinear best fit curve calculated using the sigmoidal 4PL method showed one curve for all four PD‐L1 antibodies (extra sum‐of squares F test, p = 0.2552)

Journal: Thoracic Cancer

Article Title: Harmonization across programmed death ligand 1 ( PD‐L1) assays for lung cancer by immunohistochemistry using noncontact alternating current electric field mixing

doi: 10.1111/1759-7714.13893

Figure Lengend Snippet: Comparison of the percentage of tumor cell staining for each programmed death ligand 1 (PD‐L1) assay (the 22C3, 28–8, SP263, and SP142 assays), by case, with/without alternating current (AC) mixing. (a) Conventional immunohistochemistry (IHC) ( ) 22C3, ( ) 28‐8, ( ) SP263, ( ) SP142. (b) AC mixing IHC ( ) 22C3, ( ) 28‐8 AC, ( ) SP263 AC, ( ) SP142 AC. The standard IHC is the 22C3 phamDx IHC on Autostainer Link 48 platform in both figures. Best fit colored curves enable comparison of score ranges between the four assays. When assessing PD‐L1 TPS in the cases of 22C3 PD‐L1 >1%‐positive patients using AC mixing, the nonlinear best fit curve calculated using the sigmoidal 4PL method showed one curve for all four PD‐L1 antibodies (extra sum‐of squares F test, p = 0.2552)

Article Snippet: Best fit curves data were fitted in GraphPad using the Sigmoidal 4PL option.

Techniques: Comparison, Staining, Immunohistochemistry

A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.

Journal: bioRxiv

Article Title: Oral immunization with rVSV bivalent vaccine elicits protective immune responses, including ADCC, against both SARS-CoV-2 and Influenza A viruses

doi: 10.1101/2023.07.14.549076

Figure Lengend Snippet: A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.

Article Snippet: The ID 50 was calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0.

Techniques: Neutralization, Infection, Incubation, Luciferase, Inhibition