shp53b Search Results


shp53b  (Addgene inc)
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p53 downregulation rescues neurodevelopmental defects in XLID JMS patient hiPSCs (A) Relative number of rosettes upon neural differentiation of WT-DYS0100, JMS-cl.1, and JMS-cl.1-expressing shRNA control (shCtrl) or shRNA targeting p53 (shP53a and <t>shP53b)</t> hiPSCs (n ≥ 3, biological replicates). JMS cells harbor HUWE1 p.G4310R. (B–D) mRNA expression levels of NES/NESTIN (B), TUBB3/TUJ1 (C), and DCX (D) analyzed by qRT-PCR in WT and JMS neural cells (collected at day 13) addressed by qRT-PCR. (E) Immunofluorescence analysis of the TUJ1 signal in WT-DYS0100, JMS-cl.1, JMS-cl.1 shCtrl, and JMS-cl.1 shP53a and shP53b at day 13 of neural differentiation. (F–H) qRT-PCR analysis of CDKN1A/p21 (F), GADD45α (G), and BAX (H) expression in WT and JMS neural cells (collected at day 13). All error bars indicate mean ± SEM (n = 3, biological replicates); one-way ANOVA followed by Dunnett’s post-test (A); one-tailed t test (B–D and F–H); ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001, n.s. ≥ 0.05. See also and .
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p53 downregulation rescues neurodevelopmental defects in XLID JMS patient hiPSCs (A) Relative number of rosettes upon neural differentiation of WT-DYS0100, JMS-cl.1, and JMS-cl.1-expressing shRNA control (shCtrl) or shRNA targeting p53 (shP53a and shP53b) hiPSCs (n ≥ 3, biological replicates). JMS cells harbor HUWE1 p.G4310R. (B–D) mRNA expression levels of NES/NESTIN (B), TUBB3/TUJ1 (C), and DCX (D) analyzed by qRT-PCR in WT and JMS neural cells (collected at day 13) addressed by qRT-PCR. (E) Immunofluorescence analysis of the TUJ1 signal in WT-DYS0100, JMS-cl.1, JMS-cl.1 shCtrl, and JMS-cl.1 shP53a and shP53b at day 13 of neural differentiation. (F–H) qRT-PCR analysis of CDKN1A/p21 (F), GADD45α (G), and BAX (H) expression in WT and JMS neural cells (collected at day 13). All error bars indicate mean ± SEM (n = 3, biological replicates); one-way ANOVA followed by Dunnett’s post-test (A); one-tailed t test (B–D and F–H); ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001, n.s. ≥ 0.05. See also and .

Journal: Cell Reports Medicine

Article Title: Increased p53 signaling impairs neural differentiation in HUWE1-promoted intellectual disabilities

doi: 10.1016/j.xcrm.2021.100240

Figure Lengend Snippet: p53 downregulation rescues neurodevelopmental defects in XLID JMS patient hiPSCs (A) Relative number of rosettes upon neural differentiation of WT-DYS0100, JMS-cl.1, and JMS-cl.1-expressing shRNA control (shCtrl) or shRNA targeting p53 (shP53a and shP53b) hiPSCs (n ≥ 3, biological replicates). JMS cells harbor HUWE1 p.G4310R. (B–D) mRNA expression levels of NES/NESTIN (B), TUBB3/TUJ1 (C), and DCX (D) analyzed by qRT-PCR in WT and JMS neural cells (collected at day 13) addressed by qRT-PCR. (E) Immunofluorescence analysis of the TUJ1 signal in WT-DYS0100, JMS-cl.1, JMS-cl.1 shCtrl, and JMS-cl.1 shP53a and shP53b at day 13 of neural differentiation. (F–H) qRT-PCR analysis of CDKN1A/p21 (F), GADD45α (G), and BAX (H) expression in WT and JMS neural cells (collected at day 13). All error bars indicate mean ± SEM (n = 3, biological replicates); one-way ANOVA followed by Dunnett’s post-test (A); one-tailed t test (B–D and F–H); ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001, n.s. ≥ 0.05. See also and .

Article Snippet: JMS-cl.1 and JMS-cl.2 hiPSCs were transduced with lentiviruses encloding for the non-specific control short-hairpin RNA (shControl; pLKO.1 puro (Addgene ID: 8453)) or the p53 targeting shRNAs; shP53a (shp53 pLKO.1 puro shRNA (Addgene ID: 19119) ), shP53b (shp53 pLKO.1 puro shRNA-427 (Addgene ID: 25636) ) and shP53c (shp53 pLKO.1 puro shRNA-941(Addgene ID: 25637) ).

Techniques: Expressing, shRNA, Control, Quantitative RT-PCR, Immunofluorescence, One-tailed Test

Journal: Cell Reports Medicine

Article Title: Increased p53 signaling impairs neural differentiation in HUWE1-promoted intellectual disabilities

doi: 10.1016/j.xcrm.2021.100240

Figure Lengend Snippet:

Article Snippet: JMS-cl.1 and JMS-cl.2 hiPSCs were transduced with lentiviruses encloding for the non-specific control short-hairpin RNA (shControl; pLKO.1 puro (Addgene ID: 8453)) or the p53 targeting shRNAs; shP53a (shp53 pLKO.1 puro shRNA (Addgene ID: 19119) ), shP53b (shp53 pLKO.1 puro shRNA-427 (Addgene ID: 25636) ) and shP53c (shp53 pLKO.1 puro shRNA-941(Addgene ID: 25637) ).

Techniques: Virus, Recombinant, Knock-Out, Library Quantification, RNA Sequencing, Control, Mutagenesis, shRNA, Software, Reverse Transcription, SYBR Green Assay, Imaging