Journal:

Article Title: Growth of Geobacter sulfurreducens with Acetate in Syntrophic Cooperation with Hydrogen-Oxidizing Anaerobic Partners

doi:

Figure Lengend Snippet: Hydrogen concentrations in the headspace of pure cultures of Fe(III)-reducing bacteria

Article Snippet: G. sulfurreducens (ATCC 51573), G. metallireducens (ATCC 53774), G. hydrogenophilus (ATCC 51590), Shewanella alga BRY (ATCC 51181), Shewanella sp. strain GC-29, and Desulfuromonas palmitatis (ATCC 51701) were from the Department of Microbiology, University of Massachusetts, Amherst.

Techniques:

Journal: Genome Biology and Evolution

Article Title: Unexpected Discovery of Hypermutator Phenotype Sounds the Alarm for Quality Control Strains

doi: 10.1093/gbe/evab148

Figure Lengend Snippet: Summary of Assemblies and Annotations of Shewanella putrefaciens ATCC-8071 and CGMCC-1.6515

Article Snippet: For the 16S rRNA-based phylogenetic tree, we used 16S rRNA sequences of seven Shewanella strains ( S. putrefaciens CN-32, S. putrefaciens ATCC-8071, S. putrefaciens CGMCC-1.6515, S. baltica NCTC-10737, S. baltica OS-678, S. algae MARS-14, and S. benthica KT99) and E. coli as the outgroup.

Techniques: Methylation

Journal: Genome Biology and Evolution

Article Title: Unexpected Discovery of Hypermutator Phenotype Sounds the Alarm for Quality Control Strains

doi: 10.1093/gbe/evab148

Figure Lengend Snippet: The Mutation Spectra of Shewanella putrefaciens ATCC-8071 and CGMCC-1.6515

Article Snippet: For the 16S rRNA-based phylogenetic tree, we used 16S rRNA sequences of seven Shewanella strains ( S. putrefaciens CN-32, S. putrefaciens ATCC-8071, S. putrefaciens CGMCC-1.6515, S. baltica NCTC-10737, S. baltica OS-678, S. algae MARS-14, and S. benthica KT99) and E. coli as the outgroup.

Techniques: Mutagenesis

Journal:

Article Title: The ars Detoxification System Is Advantageous but Not Required for As(V) Respiration by the Genetically Tractable Shewanella Species Strain ANA-3

doi: 10.1128/AEM.69.5.2800-2809.2003

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: ANA-3 Isolated from an As-treated wooden pier piling in a brackish estuary (Eel Pond, Woods Hole, Mass.), contains arsDABC , respires on As(V) resistant to >5 mM arsenite This study ARSB1 arsB mutant derived from ANA-3; arsB ::Kan(EZ::TN) This study S. oneidensis MR-1 Manganese-reducing strain from Oneida Lake, N.Y., sediments, type strain 32 S. algae OK-1 ATCC 51192, type strain, isolated from red algae 51 S. amazonensis SB2B Isolated from Amazon water, type strain 55 S. baltica 63 NCTC 10735, Japan, isolated from oil brine, type strain 60 Shewanella sp. strain MR-4 Isolated from the Black Sea water column, type strain 33 S. pealeana ANG-SQ1 Isolated from accessory nidamental gland of a squid, psychrotolerant, type strain 25 S. frigidimarina ACAM 591 Isolated from Antarctic Sea ice, type strain 7 S. putrefaciens 95 ATCC 8071, isolated from spoiled butter with surface taint, type strain 46 S. woodyi MS32 Isolated from seawater detritus, Alboran Sea, type strain 29 184 ATCC 8073, isolated from spoiled butter with surface taint 46 CL 256/73 NCTC 12093, isolate from human cerebrospinal fluid, type strain 18 CN32 Isolated from anaerobic subsurface core sample, New Mexico 14, 59 Plasmids and vectors pLAFR5 21.5-kb broad-host-range cosmid cloning vector; Tc r , lacZ 22 EZ::TN 1.2-kb transposon used for in vitro mutagenesis Epicentre pSALT1 pLAFR5-based 45-kb As(III) r cosmid from ANA-3 genomic DNA library; contains arsDABC , Tc r , confers resistance to As(III) and As(V) This study pSALT1-B10 As(III)-sensitive pSALT1; arsB :: kan (EZ::TN), Km r Tc r This study pSMV8 9.1-kb mobilizable suicide vector; oriR6K mobRP4 , Gm r D. Lies, Caltech parsB :: kan pSMV8 with Km r Tn-interrupted arsB gene from pSALT1-B10 This study Open in a separate window a Str r , streptomycin resistance; Nal r , nalidixic acid resistance; Tc r , tetracycline resistance; Gm r , gentamicin resistance.

Techniques: Plasmid Preparation, Cloning, Conjugation Assay, Isolation, Mutagenesis, Derivative Assay, Algae, In Vitro

Journal:

Article Title: Characterization of the Lipopolysaccharides and Capsules of Shewanella spp.

doi: 10.1128/AEM.68.9.4653-4657.2002

Figure Lengend Snippet: Shewanella strains used in this study

Article Snippet: Cultures were grown aerobically on a rotary shaker (150 rpm) at temperatures from 5 to 37°C and were harvested at a mid-exponential growth phase (optical density at 470 nm [OD 470 ] = ∼0.8). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Collection no. Characteristics S. putrefaciens CN32 ATCC BAA-453 Commonly used Fe oxide-reducing strain S. putrefaciens NCTC 10695 Reference strain for Owen's homology group I S. oneidensis MR-1 ATCC 700550 Type strain S. oneidensis DLM-7 S. algae OK-1 ATCC 51192 Type strain S. algae 136-2 NCTC 10738 Reference strain for Owen's homology group IV S. algae BrY ATCC 51181 Commonly used Fe oxide-reducing strain S. algae BCM-8 Shewanella sp. strain MR-4 Shewanella sp. strain CL 256/73 NCTC 12093 Reference strain for Owen's homology group III S. baltica 63 NCTC 10735 Type strain S. amazonensis SB2B ATCC 700329 Type strain S. frigidimarina ACAM 591 Type strain S. pealeana ANG-SQ1 ATCC 700345 Type strain S. woodyi MS32 ATCC 51908 Type strain Open in a separate window Shewanella strains used in this study

Techniques: Algae

Journal:

Article Title: Characterization of the Lipopolysaccharides and Capsules of Shewanella spp.

doi: 10.1128/AEM.68.9.4653-4657.2002

Figure Lengend Snippet: Surface properties of used Shewanella strains

Article Snippet: Cultures were grown aerobically on a rotary shaker (150 rpm) at temperatures from 5 to 37°C and were harvested at a mid-exponential growth phase (optical density at 470 nm [OD 470 ] = ∼0.8). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Collection no. Characteristics S. putrefaciens CN32 ATCC BAA-453 Commonly used Fe oxide-reducing strain S. putrefaciens NCTC 10695 Reference strain for Owen's homology group I S. oneidensis MR-1 ATCC 700550 Type strain S. oneidensis DLM-7 S. algae OK-1 ATCC 51192 Type strain S. algae 136-2 NCTC 10738 Reference strain for Owen's homology group IV S. algae BrY ATCC 51181 Commonly used Fe oxide-reducing strain S. algae BCM-8 Shewanella sp. strain MR-4 Shewanella sp. strain CL 256/73 NCTC 12093 Reference strain for Owen's homology group III S. baltica 63 NCTC 10735 Type strain S. amazonensis SB2B ATCC 700329 Type strain S. frigidimarina ACAM 591 Type strain S. pealeana ANG-SQ1 ATCC 700345 Type strain S. woodyi MS32 ATCC 51908 Type strain Open in a separate window Shewanella strains used in this study

Techniques: Algae

Journal: Veterinary World

Article Title: Characterization of putative pathogenic Shewanella algae isolated from ballast water

doi: 10.14202/vetworld.2021.678-688

Figure Lengend Snippet: Phylogenetic tree based on 16S rRNA gene sequences of S. algae Sa-BW1, Sa-BW2, Sa-BW7, Sa-BW8, and some other related Shewanella species. (a) Neighbor-joining tree. (b) Maximum-likelihood tree. GenBank accession numbers are given in parentheses.

Article Snippet: Comparison of the biochemical and enzymatic profiles displayed by the presumptive Shewanella isolates with other Shewanella type strains ( S. algae IAM 14159, S. putrefaciens ATCC 8071, and S. haliotis JCM 14758), as shown in , suggested all strains as S. algae .

Techniques: Algae

Journal: Veterinary World

Article Title: Characterization of putative pathogenic Shewanella algae isolated from ballast water

doi: 10.14202/vetworld.2021.678-688

Figure Lengend Snippet: Phylogenetic tree based on gyrB gene sequences of S. algae Sa-BW1, Sa-BW2, Sa-BW7, Sa-BW8, and some other related Shewanella species. (a) Neighbor-joining tree. (b) Maximum-likelihood tree. GenBank accession numbers are given in parentheses.

Article Snippet: Comparison of the biochemical and enzymatic profiles displayed by the presumptive Shewanella isolates with other Shewanella type strains ( S. algae IAM 14159, S. putrefaciens ATCC 8071, and S. haliotis JCM 14758), as shown in , suggested all strains as S. algae .

Techniques: Algae

Journal: Veterinary World

Article Title: Characterization of putative pathogenic Shewanella algae isolated from ballast water

doi: 10.14202/vetworld.2021.678-688

Figure Lengend Snippet: Maximum likelihood phylogenetic tree based on gyrB gene sequences of Shewanella algae Sa-BW1, Sa-BW2, Sa-BW7, Sa-BW8, and some other closely related S. algae strains. GenBank accession numbers are given in parentheses.

Article Snippet: Comparison of the biochemical and enzymatic profiles displayed by the presumptive Shewanella isolates with other Shewanella type strains ( S. algae IAM 14159, S. putrefaciens ATCC 8071, and S. haliotis JCM 14758), as shown in , suggested all strains as S. algae .

Techniques: Algae

Journal:

Article Title: Characterization of the Lipopolysaccharides and Capsules of Shewanella spp.

doi: 10.1128/AEM.68.9.4653-4657.2002

Figure Lengend Snippet: Shewanella strains used in this study

Article Snippet: Cultures were grown aerobically on a rotary shaker (150 rpm) at temperatures from 5 to 37°C and were harvested at a mid-exponential growth phase (optical density at 470 nm [OD 470 ] = ∼0.8). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Collection no. Characteristics S. putrefaciens CN32 ATCC BAA-453 Commonly used Fe oxide-reducing strain S. putrefaciens NCTC 10695 Reference strain for Owen's homology group I S. oneidensis MR-1 ATCC 700550 Type strain S. oneidensis DLM-7 S. algae OK-1 ATCC 51192 Type strain S. algae 136-2 NCTC 10738 Reference strain for Owen's homology group IV S. algae BrY ATCC 51181 Commonly used Fe oxide-reducing strain S. algae BCM-8 Shewanella sp. strain MR-4 Shewanella sp. strain CL 256/73 NCTC 12093 Reference strain for Owen's homology group III S. baltica 63 NCTC 10735 Type strain S. amazonensis SB2B ATCC 700329 Type strain S. frigidimarina ACAM 591 Type strain S. pealeana ANG-SQ1 ATCC 700345 Type strain S. woodyi MS32 ATCC 51908 Type strain Open in a separate window Shewanella strains used in this study

Techniques:

Journal:

Article Title: Characterization of the Lipopolysaccharides and Capsules of Shewanella spp.

doi: 10.1128/AEM.68.9.4653-4657.2002

Figure Lengend Snippet: Silver-stained SDS-PAGE gel of LPS from S. algae BrYFC (lane 1), S. algae 136-2 (lane 2), S. algae BCM-8 (lane 3), S. amazonensis SB2B (lane 4), S. pealeana (lane 5), and S. woodyi (lane 6) cultured at 26°C.

Article Snippet: Cultures were grown aerobically on a rotary shaker (150 rpm) at temperatures from 5 to 37°C and were harvested at a mid-exponential growth phase (optical density at 470 nm [OD 470 ] = ∼0.8). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Collection no. Characteristics S. putrefaciens CN32 ATCC BAA-453 Commonly used Fe oxide-reducing strain S. putrefaciens NCTC 10695 Reference strain for Owen's homology group I S. oneidensis MR-1 ATCC 700550 Type strain S. oneidensis DLM-7 S. algae OK-1 ATCC 51192 Type strain S. algae 136-2 NCTC 10738 Reference strain for Owen's homology group IV S. algae BrY ATCC 51181 Commonly used Fe oxide-reducing strain S. algae BCM-8 Shewanella sp. strain MR-4 Shewanella sp. strain CL 256/73 NCTC 12093 Reference strain for Owen's homology group III S. baltica 63 NCTC 10735 Type strain S. amazonensis SB2B ATCC 700329 Type strain S. frigidimarina ACAM 591 Type strain S. pealeana ANG-SQ1 ATCC 700345 Type strain S. woodyi MS32 ATCC 51908 Type strain Open in a separate window Shewanella strains used in this study

Techniques: Staining, SDS Page, Cell Culture

Journal:

Article Title: Characterization of the Lipopolysaccharides and Capsules of Shewanella spp.

doi: 10.1128/AEM.68.9.4653-4657.2002

Figure Lengend Snippet: Surface properties of used Shewanella strains

Article Snippet: Cultures were grown aerobically on a rotary shaker (150 rpm) at temperatures from 5 to 37°C and were harvested at a mid-exponential growth phase (optical density at 470 nm [OD 470 ] = ∼0.8). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Collection no. Characteristics S. putrefaciens CN32 ATCC BAA-453 Commonly used Fe oxide-reducing strain S. putrefaciens NCTC 10695 Reference strain for Owen's homology group I S. oneidensis MR-1 ATCC 700550 Type strain S. oneidensis DLM-7 S. algae OK-1 ATCC 51192 Type strain S. algae 136-2 NCTC 10738 Reference strain for Owen's homology group IV S. algae BrY ATCC 51181 Commonly used Fe oxide-reducing strain S. algae BCM-8 Shewanella sp. strain MR-4 Shewanella sp. strain CL 256/73 NCTC 12093 Reference strain for Owen's homology group III S. baltica 63 NCTC 10735 Type strain S. amazonensis SB2B ATCC 700329 Type strain S. frigidimarina ACAM 591 Type strain S. pealeana ANG-SQ1 ATCC 700345 Type strain S. woodyi MS32 ATCC 51908 Type strain Open in a separate window Shewanella strains used in this study

Techniques:

Journal:

Article Title: Characterization of the Lipopolysaccharides and Capsules of Shewanella spp.

doi: 10.1128/AEM.68.9.4653-4657.2002

Figure Lengend Snippet: All are thin sections and the bar in each image is 100 nm. (A) Conventionally embedded S. oneidensis MR-1; OM, outer membrane; P, periplasm; PM, plasma membrane. Note the absence of fibrous material on the outer membrane of this cell. (B) Freeze-substitution of S. oneidensis MR-1 (a patch of fringe on the outer membrane is shown by the arrow, whereas adjacent regions do not have the fringe). (C) Freeze-substitution of S. algae BrYFC (only one of the two cells has a fringe). (D) Freeze-substitution of Shewanella sp. strain MR-4 (arrows indicate the ordered fibrous material external to the outer membrane). (E) Freeze-substitution of S. putrefaciens CN32 (no fringe is seen).

Article Snippet: Cultures were grown aerobically on a rotary shaker (150 rpm) at temperatures from 5 to 37°C and were harvested at a mid-exponential growth phase (optical density at 470 nm [OD 470 ] = ∼0.8). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain Collection no. Characteristics S. putrefaciens CN32 ATCC BAA-453 Commonly used Fe oxide-reducing strain S. putrefaciens NCTC 10695 Reference strain for Owen's homology group I S. oneidensis MR-1 ATCC 700550 Type strain S. oneidensis DLM-7 S. algae OK-1 ATCC 51192 Type strain S. algae 136-2 NCTC 10738 Reference strain for Owen's homology group IV S. algae BrY ATCC 51181 Commonly used Fe oxide-reducing strain S. algae BCM-8 Shewanella sp. strain MR-4 Shewanella sp. strain CL 256/73 NCTC 12093 Reference strain for Owen's homology group III S. baltica 63 NCTC 10735 Type strain S. amazonensis SB2B ATCC 700329 Type strain S. frigidimarina ACAM 591 Type strain S. pealeana ANG-SQ1 ATCC 700345 Type strain S. woodyi MS32 ATCC 51908 Type strain Open in a separate window Shewanella strains used in this study

Techniques: