serpine2 Search Results


gene exp serpine2 mm00436753 m1  (Thermo Fisher)
86
Thermo Fisher gene exp serpine2 mm00436753 m1
Gene Exp Serpine2 Mm00436753 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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serpine2 rabbit polyclonal antibody  (Proteintech)
93
Proteintech serpine2 rabbit polyclonal antibody
BMP6 signaling enhancement compensates for shallow trophoblast invasion in PE. An examination of clinical samples from PE patients and their gestational age-matched controls revealed that BMP6 was upregulated in preeclamptic placentas. BMP6 enhances trophoblast invasion via ID1-mediated upregulation of <t>SERPINE2</t> and PlGF. Moreover, BMP6 also intensifies trophoblast vascular mimicry through the ID1-mediated upregulation of PlGF. Both canonical (p-SMAD1/5/9) and noncanonical (p-SMAD2/3) pathways participate in BMP6-induced ID1 expression. Our findings indicate that the increase in BMP6 signaling during late gestation could serve as a compensatory response to shallow trophoblast invasion in PE, which in light of BMP6 and its downstream targets could serve as diagnostic markers and therapeutic target applications in the clinical management of PE. This schematic diagram was created with Biorender.com
Serpine2 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
serpine2 rabbit polyclonal antibody - by Bioz Stars, 2026-06
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serpine2 concentration  (Cusabio)
94
Cusabio serpine2 concentration
A Volcano plot of differentially expressed genes in SBC-2 cells with stable RASA4 knockdown, relative to those transfected with scramble shRNA. B qRT-PCR shows that <t>SERPINE2</t> mRNA levels were significantly lower in DMS114 and SHP77 cells with stable over-expression of RASA4, compared to cells stably transfected with an empty vector. SERPINE2 mRNA levels were significantly higher in H446 and SBC-2 cells with stable knockdown of RASA4, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). C ELISA analysis shows that H446 and SBC-2 cells with RASA4 knockdown secreted more SERPINE2 protein to cell culture medium, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). D Immunofluorescence staining of SERPINE2 protein in SBC-2 and H446 cells with stable transfections of scramble shRNA and RASA4 shRNA. (scale bar = 10 μm for SBC-2 cells, scale bar = 20 μm for H446 cells). E Co-IP analysis of protein interaction between RASA4 and SERPINE2 in DMS114 cells with RASA4 stable over-expression. Cell lysates from DMS114 cells with stable transfection of FLAG-tagged RASA4 were immunoprecipitated with anti-FLAG antibody, followed by western blot with anti-SERPINE2 antibody. IgG was used as a negative control. F Western blot shows that the protein expression of SERPINE2 and EMT markers (N-cadherin, E-cadherin, Vinmintin, and snail) following RASA4 stable knockdown and overexpression in SBC-2 and DMS114 cells. G qRT-PCR analysis of stemness markers NANOG , OCT4 , SOX2, and MYC in SBC-2 and DMS114 cells with RASA4 stable knockdown and overexpression. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). H Sphere formation assay in SBC-2 and H446 cells with RASA4 stable knockdown, and in DMS114 and SHP77 cells with RASA4 stable overexpression. (scale bar = 50 μm).
Serpine2 Concentration, supplied by Cusabio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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serpine2 rnai lentivirus lv rnai  (Taconic Biosciences)
85
Taconic Biosciences serpine2 rnai lentivirus lv rnai
A Volcano plot of differentially expressed genes in SBC-2 cells with stable RASA4 knockdown, relative to those transfected with scramble shRNA. B qRT-PCR shows that <t>SERPINE2</t> mRNA levels were significantly lower in DMS114 and SHP77 cells with stable over-expression of RASA4, compared to cells stably transfected with an empty vector. SERPINE2 mRNA levels were significantly higher in H446 and SBC-2 cells with stable knockdown of RASA4, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). C ELISA analysis shows that H446 and SBC-2 cells with RASA4 knockdown secreted more SERPINE2 protein to cell culture medium, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). D Immunofluorescence staining of SERPINE2 protein in SBC-2 and H446 cells with stable transfections of scramble shRNA and RASA4 shRNA. (scale bar = 10 μm for SBC-2 cells, scale bar = 20 μm for H446 cells). E Co-IP analysis of protein interaction between RASA4 and SERPINE2 in DMS114 cells with RASA4 stable over-expression. Cell lysates from DMS114 cells with stable transfection of FLAG-tagged RASA4 were immunoprecipitated with anti-FLAG antibody, followed by western blot with anti-SERPINE2 antibody. IgG was used as a negative control. F Western blot shows that the protein expression of SERPINE2 and EMT markers (N-cadherin, E-cadherin, Vinmintin, and snail) following RASA4 stable knockdown and overexpression in SBC-2 and DMS114 cells. G qRT-PCR analysis of stemness markers NANOG , OCT4 , SOX2, and MYC in SBC-2 and DMS114 cells with RASA4 stable knockdown and overexpression. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). H Sphere formation assay in SBC-2 and H446 cells with RASA4 stable knockdown, and in DMS114 and SHP77 cells with RASA4 stable overexpression. (scale bar = 50 μm).
Serpine2 Rnai Lentivirus Lv Rnai, supplied by Taconic Biosciences, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
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human glia derived nexin serpine2 elisa kit  (Cusabio)
94
Cusabio human glia derived nexin serpine2 elisa kit
A Volcano plot of differentially expressed genes in SBC-2 cells with stable RASA4 knockdown, relative to those transfected with scramble shRNA. B qRT-PCR shows that <t>SERPINE2</t> mRNA levels were significantly lower in DMS114 and SHP77 cells with stable over-expression of RASA4, compared to cells stably transfected with an empty vector. SERPINE2 mRNA levels were significantly higher in H446 and SBC-2 cells with stable knockdown of RASA4, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). C ELISA analysis shows that H446 and SBC-2 cells with RASA4 knockdown secreted more SERPINE2 protein to cell culture medium, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). D Immunofluorescence staining of SERPINE2 protein in SBC-2 and H446 cells with stable transfections of scramble shRNA and RASA4 shRNA. (scale bar = 10 μm for SBC-2 cells, scale bar = 20 μm for H446 cells). E Co-IP analysis of protein interaction between RASA4 and SERPINE2 in DMS114 cells with RASA4 stable over-expression. Cell lysates from DMS114 cells with stable transfection of FLAG-tagged RASA4 were immunoprecipitated with anti-FLAG antibody, followed by western blot with anti-SERPINE2 antibody. IgG was used as a negative control. F Western blot shows that the protein expression of SERPINE2 and EMT markers (N-cadherin, E-cadherin, Vinmintin, and snail) following RASA4 stable knockdown and overexpression in SBC-2 and DMS114 cells. G qRT-PCR analysis of stemness markers NANOG , OCT4 , SOX2, and MYC in SBC-2 and DMS114 cells with RASA4 stable knockdown and overexpression. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). H Sphere formation assay in SBC-2 and H446 cells with RASA4 stable knockdown, and in DMS114 and SHP77 cells with RASA4 stable overexpression. (scale bar = 50 μm).
Human Glia Derived Nexin Serpine2 Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
human glia derived nexin serpine2 elisa kit - by Bioz Stars, 2026-06
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pai  (Boster Bio)
93
Boster Bio pai
A Volcano plot of differentially expressed genes in SBC-2 cells with stable RASA4 knockdown, relative to those transfected with scramble shRNA. B qRT-PCR shows that <t>SERPINE2</t> mRNA levels were significantly lower in DMS114 and SHP77 cells with stable over-expression of RASA4, compared to cells stably transfected with an empty vector. SERPINE2 mRNA levels were significantly higher in H446 and SBC-2 cells with stable knockdown of RASA4, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). C ELISA analysis shows that H446 and SBC-2 cells with RASA4 knockdown secreted more SERPINE2 protein to cell culture medium, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). D Immunofluorescence staining of SERPINE2 protein in SBC-2 and H446 cells with stable transfections of scramble shRNA and RASA4 shRNA. (scale bar = 10 μm for SBC-2 cells, scale bar = 20 μm for H446 cells). E Co-IP analysis of protein interaction between RASA4 and SERPINE2 in DMS114 cells with RASA4 stable over-expression. Cell lysates from DMS114 cells with stable transfection of FLAG-tagged RASA4 were immunoprecipitated with anti-FLAG antibody, followed by western blot with anti-SERPINE2 antibody. IgG was used as a negative control. F Western blot shows that the protein expression of SERPINE2 and EMT markers (N-cadherin, E-cadherin, Vinmintin, and snail) following RASA4 stable knockdown and overexpression in SBC-2 and DMS114 cells. G qRT-PCR analysis of stemness markers NANOG , OCT4 , SOX2, and MYC in SBC-2 and DMS114 cells with RASA4 stable knockdown and overexpression. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). H Sphere formation assay in SBC-2 and H446 cells with RASA4 stable knockdown, and in DMS114 and SHP77 cells with RASA4 stable overexpression. (scale bar = 50 μm).
Pai, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pai/product/Boster Bio
Average 93 stars, based on 1 article reviews
pai - by Bioz Stars, 2026-06
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gene exp serpine2 hs00385730 m1  (Thermo Fisher)
85
Thermo Fisher gene exp serpine2 hs00385730 m1
A Volcano plot of differentially expressed genes in SBC-2 cells with stable RASA4 knockdown, relative to those transfected with scramble shRNA. B qRT-PCR shows that <t>SERPINE2</t> mRNA levels were significantly lower in DMS114 and SHP77 cells with stable over-expression of RASA4, compared to cells stably transfected with an empty vector. SERPINE2 mRNA levels were significantly higher in H446 and SBC-2 cells with stable knockdown of RASA4, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). C ELISA analysis shows that H446 and SBC-2 cells with RASA4 knockdown secreted more SERPINE2 protein to cell culture medium, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). D Immunofluorescence staining of SERPINE2 protein in SBC-2 and H446 cells with stable transfections of scramble shRNA and RASA4 shRNA. (scale bar = 10 μm for SBC-2 cells, scale bar = 20 μm for H446 cells). E Co-IP analysis of protein interaction between RASA4 and SERPINE2 in DMS114 cells with RASA4 stable over-expression. Cell lysates from DMS114 cells with stable transfection of FLAG-tagged RASA4 were immunoprecipitated with anti-FLAG antibody, followed by western blot with anti-SERPINE2 antibody. IgG was used as a negative control. F Western blot shows that the protein expression of SERPINE2 and EMT markers (N-cadherin, E-cadherin, Vinmintin, and snail) following RASA4 stable knockdown and overexpression in SBC-2 and DMS114 cells. G qRT-PCR analysis of stemness markers NANOG , OCT4 , SOX2, and MYC in SBC-2 and DMS114 cells with RASA4 stable knockdown and overexpression. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). H Sphere formation assay in SBC-2 and H446 cells with RASA4 stable knockdown, and in DMS114 and SHP77 cells with RASA4 stable overexpression. (scale bar = 50 μm).
Gene Exp Serpine2 Hs00385730 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp serpine2 hs00385730 m1/product/Thermo Fisher
Average 85 stars, based on 1 article reviews
gene exp serpine2 hs00385730 m1 - by Bioz Stars, 2026-06
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immunohistochemical staining anti human serpine2 antibody  (Proteintech)
91
Proteintech immunohistochemical staining anti human serpine2 antibody
A Volcano plot of differentially expressed genes in SBC-2 cells with stable RASA4 knockdown, relative to those transfected with scramble shRNA. B qRT-PCR shows that <t>SERPINE2</t> mRNA levels were significantly lower in DMS114 and SHP77 cells with stable over-expression of RASA4, compared to cells stably transfected with an empty vector. SERPINE2 mRNA levels were significantly higher in H446 and SBC-2 cells with stable knockdown of RASA4, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). C ELISA analysis shows that H446 and SBC-2 cells with RASA4 knockdown secreted more SERPINE2 protein to cell culture medium, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). D Immunofluorescence staining of SERPINE2 protein in SBC-2 and H446 cells with stable transfections of scramble shRNA and RASA4 shRNA. (scale bar = 10 μm for SBC-2 cells, scale bar = 20 μm for H446 cells). E Co-IP analysis of protein interaction between RASA4 and SERPINE2 in DMS114 cells with RASA4 stable over-expression. Cell lysates from DMS114 cells with stable transfection of FLAG-tagged RASA4 were immunoprecipitated with anti-FLAG antibody, followed by western blot with anti-SERPINE2 antibody. IgG was used as a negative control. F Western blot shows that the protein expression of SERPINE2 and EMT markers (N-cadherin, E-cadherin, Vinmintin, and snail) following RASA4 stable knockdown and overexpression in SBC-2 and DMS114 cells. G qRT-PCR analysis of stemness markers NANOG , OCT4 , SOX2, and MYC in SBC-2 and DMS114 cells with RASA4 stable knockdown and overexpression. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). H Sphere formation assay in SBC-2 and H446 cells with RASA4 stable knockdown, and in DMS114 and SHP77 cells with RASA4 stable overexpression. (scale bar = 50 μm).
Immunohistochemical Staining Anti Human Serpine2 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunohistochemical staining anti human serpine2 antibody - by Bioz Stars, 2026-06
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serpine2 human  (Cusabio)
93
Cusabio serpine2 human
<t>SERPINE2</t> expression is significantly upregulated in COAD. (A) Box plot of SERPINE family genes mRNA expression.in COAD(p < 0.05). (B) Box plot of SERPINE1 and SERPINE2 protein expression in COAD(p < 0.05). (C) Box plot of SERPINE2 mRNA expression among groups of different tumor grades in COAD (p < 0.05).
Serpine2 Human, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
serpine2 human - by Bioz Stars, 2026-06
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serpine2 bio his  (Addgene inc)
85
Addgene inc serpine2 bio his
<t>SERPINE2</t> expression is significantly upregulated in COAD. (A) Box plot of SERPINE family genes mRNA expression.in COAD(p < 0.05). (B) Box plot of SERPINE1 and SERPINE2 protein expression in COAD(p < 0.05). (C) Box plot of SERPINE2 mRNA expression among groups of different tumor grades in COAD (p < 0.05).
Serpine2 Bio His, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-human serpine2 antibody  (Abnova)
90
Abnova anti-human serpine2 antibody
Antibody specificity and presence of the <t>SERPINE2</t> protein in human uterine fluid . (A) Four hundred nanograms of recombinant human SERPINE2 was resolved on 10% SDS-PAGE and followed by Western blotting using anti-mouse SERPINE2 antiserum (lane 1), an anti-human SERPINE2 antibody (R&D) (lane2), or another anti-human SERPINE2 antibody (Abnova) (lane 3). (B) One hundred micrograms of the extract of endometrial curettage was analyzed by anti-mouse SERPINE2 antiserum (lanes 1 and 2) and an anti-human SERPINE2 antibody (R&D) (lanes 3 and 4). (C) Fifty micrograms of uterine fluid proteins collected from each individual patient ( n = 7) was Western-blotted using anti-mouse SERPINE2 antiserum (1:3000) (upper panel). EP, MP, and LP indicate early-, mid-, and late-proliferative phases, and ES and MS indicate early- and mid-secretory phases, respectively. The blot was also overexposed to clearly display the staining signal (lower panel).
Anti Human Serpine2 Antibody, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


BMP6 signaling enhancement compensates for shallow trophoblast invasion in PE. An examination of clinical samples from PE patients and their gestational age-matched controls revealed that BMP6 was upregulated in preeclamptic placentas. BMP6 enhances trophoblast invasion via ID1-mediated upregulation of SERPINE2 and PlGF. Moreover, BMP6 also intensifies trophoblast vascular mimicry through the ID1-mediated upregulation of PlGF. Both canonical (p-SMAD1/5/9) and noncanonical (p-SMAD2/3) pathways participate in BMP6-induced ID1 expression. Our findings indicate that the increase in BMP6 signaling during late gestation could serve as a compensatory response to shallow trophoblast invasion in PE, which in light of BMP6 and its downstream targets could serve as diagnostic markers and therapeutic target applications in the clinical management of PE. This schematic diagram was created with Biorender.com

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: BMP6 as a therapeutic target for preeclampsia: enhancing trophoblast invasion and vascular mimicry

doi: 10.1007/s00018-025-06040-w

Figure Lengend Snippet: BMP6 signaling enhancement compensates for shallow trophoblast invasion in PE. An examination of clinical samples from PE patients and their gestational age-matched controls revealed that BMP6 was upregulated in preeclamptic placentas. BMP6 enhances trophoblast invasion via ID1-mediated upregulation of SERPINE2 and PlGF. Moreover, BMP6 also intensifies trophoblast vascular mimicry through the ID1-mediated upregulation of PlGF. Both canonical (p-SMAD1/5/9) and noncanonical (p-SMAD2/3) pathways participate in BMP6-induced ID1 expression. Our findings indicate that the increase in BMP6 signaling during late gestation could serve as a compensatory response to shallow trophoblast invasion in PE, which in light of BMP6 and its downstream targets could serve as diagnostic markers and therapeutic target applications in the clinical management of PE. This schematic diagram was created with Biorender.com

Article Snippet: SMAD1 (D59D7) rabbit monoclonal antibody (#6944), phospho-SMAD1/5/9 (D5B10) rabbit monoclonal antibody (#13820), SMAD2 (D43B4) rabbit monoclonal antibody (#5339), phospho-SMAD2 (E8F3R) rabbit monoclonal antibody (#18338), SMAD3 (C67H9) rabbit monoclonal antibody (#9523), phospho-SMAD3 (C25A9) rabbit monoclonal antibody (# 9520), SMAD4 (D3R4N) rabbit monoclonal antibody (#46535), horseradish peroxidase (HRP)-linked anti-mouse IgG (#7076), and anti-rabbit IgG (#7074) were purchased from Cell Signaling Technology (Danvers, MA, USA); the mouse monoclonal antibody HLA-G (#11–499-C100) was purchased from EXBIO (Vestec, Czech Republic); CK7-specific rabbit polyclonal antibody (#17513-1-AP), BMP6 rabbit polyclonal antibody (#55421-1-AP), SERPINE2 rabbit polyclonal antibody (#11303-1-AP), and α-Tubulin mouse monoclonal antibody (#66031-1-Ig) were purchased from Proteintech (Wuhan, China); ID1 mouse monoclonal antibody (#sc-133104) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA); CD34 rabbit monoclonal antibody (#A19015) and α-SMA rabbit monoclonal antibody (#A2235) were obtained from ABclonal (Wuhan, China); Goat anti-Rabbit IgG secondary antibody, Alexa Fluor 594 (#A-11012) and goat anti-Mouse IgG secondary antibody, Alexa Fluor 488 (#A-11011) were purchased from Thermo Fisher (NY, USA).

Techniques: Expressing, Diagnostic Assay

ID1 mediates BMP6-induced SERPINE2 and PlGF upregulation in human trophoblasts. A-C , BMP6 upregulates SERPINE2 protein levels in trophoblasts. A , HTR8/SVneo cells were treated with different concentrations (0, 6.25, 12.5, 25, 50, or 100 ng/mL) of BMP6, and the SERPINE2 protein levels after 24 h of treatment were examined by Western blot analysis. The upper panel shows a representative Western blot image, and the lower panel shows the summarized quantitative results. B , SERPINE2 protein levels in HTR8/SVneo cells after treatment with vehicle (Ctrl) or 50 ng/mL BMP6 for different durations (24, 48, and 72 h). The upper panel shows a representative Western blot image, and the lower panel shows the summarized quantitative results. C , SERPINE2 protein levels in human primary EVTs. The left panel shows a representative Western blot image, and the right panel shows the summarized quantitative results. D-E , BMP6 promotes PlGF accumulation in the conditioned medium of trophoblasts. D , HTR8/SVneo cells were treated with or without 50 ng/mL BMP6 for 24–48 h. PlGF accumulation in conditioned medium was measured using ELISA. E , PlGF accumulation in conditioned medium was assayed by ELISA 48 h after BMP6 treatment in primary EVTs. F-J , ID1 mediates BMP6-induced SERPINE2 and PlGF upregulation in human trophoblasts. HTR8/SVneo cells were transfected for 48 h with 20 nM control non-targeting siRNA (si-Ctrl) or siRNA targeting ID1 (si- ID1 ) before treatment with or without 50 ng/mL BMP6. F , ID1 mRNA levels were examined by qPCR 6 h after BMP6 (50 ng/mL) treatment in HTR8/SVneo cells, with GAPDH used as the reference gene. G and H , SERPINE2 and ID1 protein levels in HTR8/SVneo cells ( G ) and human primary EVTs ( H ) after transfection with siRNA targeting ID1 , followed by treatment with or without BMP6 for 24 h, as assessed by Western blot. The left panel shows a representative Western blot image, and the right panel shows the summarized quantitative results. I , PGF mRNA levels were examined by qPCR 6 h after BMP6 treatment in HTR8/SVneo cells, with GAPDH used as the reference gene. J , PlGF accumulation in conditioned medium was assayed by ELISA 24 h after BMP6 treatment in HTR8/SVneo cells. K , SMAD4 mediates BMP6-induced upregulation of PlGF in human trophoblasts. HTR8/SVneo cells were transfected for 48 h with 20 nM control non-targeting siRNA (si-Ctrl) or siRNA targeting SMAD4 (si- SMAD4 ) before treatment with or without 50 ng/mL BMP6. PlGF accumulation in conditioned medium was assayed by ELISA 24 h after BMP6 treatment in HTR8/SVneo cells. The quantitative results are expressed as the means ± SEMs of at least three independent experiments. One-way ANOVA was used for analyses in A , and two-way ANOVA was used for comparisons in B-K . Groups without common letters are significantly different from each other ( P < 0.05). BMP6, bone morphogenetic protein 6; SERPINE2, serpin family E member 2; EVT, extravillous cytotrophoblast; Ctrl, control; PlGF, placental growth factor; ID1, inhibitor of DNA-binding 1

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: BMP6 as a therapeutic target for preeclampsia: enhancing trophoblast invasion and vascular mimicry

doi: 10.1007/s00018-025-06040-w

Figure Lengend Snippet: ID1 mediates BMP6-induced SERPINE2 and PlGF upregulation in human trophoblasts. A-C , BMP6 upregulates SERPINE2 protein levels in trophoblasts. A , HTR8/SVneo cells were treated with different concentrations (0, 6.25, 12.5, 25, 50, or 100 ng/mL) of BMP6, and the SERPINE2 protein levels after 24 h of treatment were examined by Western blot analysis. The upper panel shows a representative Western blot image, and the lower panel shows the summarized quantitative results. B , SERPINE2 protein levels in HTR8/SVneo cells after treatment with vehicle (Ctrl) or 50 ng/mL BMP6 for different durations (24, 48, and 72 h). The upper panel shows a representative Western blot image, and the lower panel shows the summarized quantitative results. C , SERPINE2 protein levels in human primary EVTs. The left panel shows a representative Western blot image, and the right panel shows the summarized quantitative results. D-E , BMP6 promotes PlGF accumulation in the conditioned medium of trophoblasts. D , HTR8/SVneo cells were treated with or without 50 ng/mL BMP6 for 24–48 h. PlGF accumulation in conditioned medium was measured using ELISA. E , PlGF accumulation in conditioned medium was assayed by ELISA 48 h after BMP6 treatment in primary EVTs. F-J , ID1 mediates BMP6-induced SERPINE2 and PlGF upregulation in human trophoblasts. HTR8/SVneo cells were transfected for 48 h with 20 nM control non-targeting siRNA (si-Ctrl) or siRNA targeting ID1 (si- ID1 ) before treatment with or without 50 ng/mL BMP6. F , ID1 mRNA levels were examined by qPCR 6 h after BMP6 (50 ng/mL) treatment in HTR8/SVneo cells, with GAPDH used as the reference gene. G and H , SERPINE2 and ID1 protein levels in HTR8/SVneo cells ( G ) and human primary EVTs ( H ) after transfection with siRNA targeting ID1 , followed by treatment with or without BMP6 for 24 h, as assessed by Western blot. The left panel shows a representative Western blot image, and the right panel shows the summarized quantitative results. I , PGF mRNA levels were examined by qPCR 6 h after BMP6 treatment in HTR8/SVneo cells, with GAPDH used as the reference gene. J , PlGF accumulation in conditioned medium was assayed by ELISA 24 h after BMP6 treatment in HTR8/SVneo cells. K , SMAD4 mediates BMP6-induced upregulation of PlGF in human trophoblasts. HTR8/SVneo cells were transfected for 48 h with 20 nM control non-targeting siRNA (si-Ctrl) or siRNA targeting SMAD4 (si- SMAD4 ) before treatment with or without 50 ng/mL BMP6. PlGF accumulation in conditioned medium was assayed by ELISA 24 h after BMP6 treatment in HTR8/SVneo cells. The quantitative results are expressed as the means ± SEMs of at least three independent experiments. One-way ANOVA was used for analyses in A , and two-way ANOVA was used for comparisons in B-K . Groups without common letters are significantly different from each other ( P < 0.05). BMP6, bone morphogenetic protein 6; SERPINE2, serpin family E member 2; EVT, extravillous cytotrophoblast; Ctrl, control; PlGF, placental growth factor; ID1, inhibitor of DNA-binding 1

Article Snippet: SMAD1 (D59D7) rabbit monoclonal antibody (#6944), phospho-SMAD1/5/9 (D5B10) rabbit monoclonal antibody (#13820), SMAD2 (D43B4) rabbit monoclonal antibody (#5339), phospho-SMAD2 (E8F3R) rabbit monoclonal antibody (#18338), SMAD3 (C67H9) rabbit monoclonal antibody (#9523), phospho-SMAD3 (C25A9) rabbit monoclonal antibody (# 9520), SMAD4 (D3R4N) rabbit monoclonal antibody (#46535), horseradish peroxidase (HRP)-linked anti-mouse IgG (#7076), and anti-rabbit IgG (#7074) were purchased from Cell Signaling Technology (Danvers, MA, USA); the mouse monoclonal antibody HLA-G (#11–499-C100) was purchased from EXBIO (Vestec, Czech Republic); CK7-specific rabbit polyclonal antibody (#17513-1-AP), BMP6 rabbit polyclonal antibody (#55421-1-AP), SERPINE2 rabbit polyclonal antibody (#11303-1-AP), and α-Tubulin mouse monoclonal antibody (#66031-1-Ig) were purchased from Proteintech (Wuhan, China); ID1 mouse monoclonal antibody (#sc-133104) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA); CD34 rabbit monoclonal antibody (#A19015) and α-SMA rabbit monoclonal antibody (#A2235) were obtained from ABclonal (Wuhan, China); Goat anti-Rabbit IgG secondary antibody, Alexa Fluor 594 (#A-11012) and goat anti-Mouse IgG secondary antibody, Alexa Fluor 488 (#A-11011) were purchased from Thermo Fisher (NY, USA).

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Transfection, Control, Binding Assay

Both SERPINE2 and PlGF mediate BMP6-induced trophoblast invasion. A-J , HTR8/SVneo cells or primary EVTs were transfected for 48 h with 20 nM control nontargeting siRNA (si-Ctrl), 20 nM siRNA targeting SERPINE2 (si- SERPINE2 ) or PGF (si- PGF ) before treatment with or without 50 ng/mL BMP6 for 24 h. A and B , The protein levels of SERPINE2 in HTR8/SVneo cells ( A ) and human primary EVTs ( B ) after 24 h of BMP6 treatment. The upper panel shows a representative Western blot image, and the lower panel shows the summarized quantitative results. C , PlGF accumulation in conditioned medium with or without BMP6 treatment for 24 h was assayed by ELISA in HTR8/SVneo cells. D , PGF mRNA levels in human primary EVTs with or without BMP6 treatment for 6 h were examined by RT‒qPCR, with GAPDH as the reference gene. E - H , Transwell assays were employed to examine the invasiveness of HTR8/SVneo cells ( E and G ) and primary EVTs ( F and H ) with or without BMP6 treatment for 36 h. I and J , Endothelial-like tube formation assays were used to assess vascular mimicry of HTR8/SVneo cells with or without BMP6 treatment for 12 h. Representative images from the endothelial-like tube formation assay are displayed in the above panel; the summarized quantitative results are displayed in the lower panel. Scale bar, 100 μm. The quantitative results are expressed as the means ± SEMs of at least three independent experiments. Two-way ANOVA was used for data comparison. Groups without common letters are significantly different from each other ( P < 0.05). BMP6, bone morphogenetic protein 6; SERPINE2, serpin family E member 2; PGF, placental growth factor; Ctrl, control; EVT, extravillous cytotrophoblast

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: BMP6 as a therapeutic target for preeclampsia: enhancing trophoblast invasion and vascular mimicry

doi: 10.1007/s00018-025-06040-w

Figure Lengend Snippet: Both SERPINE2 and PlGF mediate BMP6-induced trophoblast invasion. A-J , HTR8/SVneo cells or primary EVTs were transfected for 48 h with 20 nM control nontargeting siRNA (si-Ctrl), 20 nM siRNA targeting SERPINE2 (si- SERPINE2 ) or PGF (si- PGF ) before treatment with or without 50 ng/mL BMP6 for 24 h. A and B , The protein levels of SERPINE2 in HTR8/SVneo cells ( A ) and human primary EVTs ( B ) after 24 h of BMP6 treatment. The upper panel shows a representative Western blot image, and the lower panel shows the summarized quantitative results. C , PlGF accumulation in conditioned medium with or without BMP6 treatment for 24 h was assayed by ELISA in HTR8/SVneo cells. D , PGF mRNA levels in human primary EVTs with or without BMP6 treatment for 6 h were examined by RT‒qPCR, with GAPDH as the reference gene. E - H , Transwell assays were employed to examine the invasiveness of HTR8/SVneo cells ( E and G ) and primary EVTs ( F and H ) with or without BMP6 treatment for 36 h. I and J , Endothelial-like tube formation assays were used to assess vascular mimicry of HTR8/SVneo cells with or without BMP6 treatment for 12 h. Representative images from the endothelial-like tube formation assay are displayed in the above panel; the summarized quantitative results are displayed in the lower panel. Scale bar, 100 μm. The quantitative results are expressed as the means ± SEMs of at least three independent experiments. Two-way ANOVA was used for data comparison. Groups without common letters are significantly different from each other ( P < 0.05). BMP6, bone morphogenetic protein 6; SERPINE2, serpin family E member 2; PGF, placental growth factor; Ctrl, control; EVT, extravillous cytotrophoblast

Article Snippet: SMAD1 (D59D7) rabbit monoclonal antibody (#6944), phospho-SMAD1/5/9 (D5B10) rabbit monoclonal antibody (#13820), SMAD2 (D43B4) rabbit monoclonal antibody (#5339), phospho-SMAD2 (E8F3R) rabbit monoclonal antibody (#18338), SMAD3 (C67H9) rabbit monoclonal antibody (#9523), phospho-SMAD3 (C25A9) rabbit monoclonal antibody (# 9520), SMAD4 (D3R4N) rabbit monoclonal antibody (#46535), horseradish peroxidase (HRP)-linked anti-mouse IgG (#7076), and anti-rabbit IgG (#7074) were purchased from Cell Signaling Technology (Danvers, MA, USA); the mouse monoclonal antibody HLA-G (#11–499-C100) was purchased from EXBIO (Vestec, Czech Republic); CK7-specific rabbit polyclonal antibody (#17513-1-AP), BMP6 rabbit polyclonal antibody (#55421-1-AP), SERPINE2 rabbit polyclonal antibody (#11303-1-AP), and α-Tubulin mouse monoclonal antibody (#66031-1-Ig) were purchased from Proteintech (Wuhan, China); ID1 mouse monoclonal antibody (#sc-133104) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA); CD34 rabbit monoclonal antibody (#A19015) and α-SMA rabbit monoclonal antibody (#A2235) were obtained from ABclonal (Wuhan, China); Goat anti-Rabbit IgG secondary antibody, Alexa Fluor 594 (#A-11012) and goat anti-Mouse IgG secondary antibody, Alexa Fluor 488 (#A-11011) were purchased from Thermo Fisher (NY, USA).

Techniques: Transfection, Control, Western Blot, Enzyme-linked Immunosorbent Assay, Tube Formation Assay, Comparison

BMP6 is elevated in patients with PE and in PE model rats. A-C , BMP6 is elevated in patients with PE. A , RT‒qPCR analysis of BMP6 mRNA expression levels in the placentas of control women ( n = 10) and PE patients ( n = 10), with GAPDH as the reference gene. B , Western blot analysis of BMP6 protein expression levels in the placentas of control women ( n = 4) and PE patients ( n = 4). C , Spearman correlation analysis between the placental BMP6 mRNA levels and the value of log10 (SBP) of the corresponding patients. The gray area represents the 95% CI. Each dot represents one sample. D , The animal experimental protocol. E and F , BMP6 is elevated in the plasma of PE model rats. Rat plasma levels of BMP6 ( E ) and PlGF ( F ) in the Ad Fc + PBS group ( n = 4) and Ad Flt1 + PBS group ( n = 4). G-M , BMP6 is elevated in the placenta of PE model rats at G13. RNA-seq analysis of rat placentas at G13 in the Ad Fc + PBS group ( n = 3) and Ad Flt1 + PBS group ( n = 3). G , Heatmap depicting DEGs in the two groups. H , Dot plots of significantly enriched GO terms; the dot size represents the number of DEGs associated with a particular GO term. I , KEGG hierarchical network plot of pathways. J , GSEA-KEGG Ridge plot of pathways. K , GSEA plots of cytokine-cytokine receptor interaction pathway. L , Volcano plot of RNA-seq data showing DEGs between the Ad Fc + PBS group and the Ad Flt1 + PBS group. M , Circos graph displaying the coexpression networks of five genes in rat placenta samples. Each sector of the circle represents one gene, and its width indicates the total amount of co-occurrence that connects one gene to the other. The width of each link represents the total number of coexpressed genes among the linked genes. Student’s t-test was used for comparisons between two groups in A , B , E , and F . Groups without common letters are significantly different from each other ( P < 0.05). SD, Sprague–Dawley; BMP6, bone morphogenetic protein 6; PlGF, placental growth factor; PBS, phosphate-buffered saline; Ad Flt1, adenovirus expressing fms-like tyrosine kinase-1; Ad Fc, adenovirus-expressing control IgG2a Fc fragment; FC, fold change; Serpine2, serpin family E member 2; Id1, inhibitor of DNA-binding 1

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: BMP6 as a therapeutic target for preeclampsia: enhancing trophoblast invasion and vascular mimicry

doi: 10.1007/s00018-025-06040-w

Figure Lengend Snippet: BMP6 is elevated in patients with PE and in PE model rats. A-C , BMP6 is elevated in patients with PE. A , RT‒qPCR analysis of BMP6 mRNA expression levels in the placentas of control women ( n = 10) and PE patients ( n = 10), with GAPDH as the reference gene. B , Western blot analysis of BMP6 protein expression levels in the placentas of control women ( n = 4) and PE patients ( n = 4). C , Spearman correlation analysis between the placental BMP6 mRNA levels and the value of log10 (SBP) of the corresponding patients. The gray area represents the 95% CI. Each dot represents one sample. D , The animal experimental protocol. E and F , BMP6 is elevated in the plasma of PE model rats. Rat plasma levels of BMP6 ( E ) and PlGF ( F ) in the Ad Fc + PBS group ( n = 4) and Ad Flt1 + PBS group ( n = 4). G-M , BMP6 is elevated in the placenta of PE model rats at G13. RNA-seq analysis of rat placentas at G13 in the Ad Fc + PBS group ( n = 3) and Ad Flt1 + PBS group ( n = 3). G , Heatmap depicting DEGs in the two groups. H , Dot plots of significantly enriched GO terms; the dot size represents the number of DEGs associated with a particular GO term. I , KEGG hierarchical network plot of pathways. J , GSEA-KEGG Ridge plot of pathways. K , GSEA plots of cytokine-cytokine receptor interaction pathway. L , Volcano plot of RNA-seq data showing DEGs between the Ad Fc + PBS group and the Ad Flt1 + PBS group. M , Circos graph displaying the coexpression networks of five genes in rat placenta samples. Each sector of the circle represents one gene, and its width indicates the total amount of co-occurrence that connects one gene to the other. The width of each link represents the total number of coexpressed genes among the linked genes. Student’s t-test was used for comparisons between two groups in A , B , E , and F . Groups without common letters are significantly different from each other ( P < 0.05). SD, Sprague–Dawley; BMP6, bone morphogenetic protein 6; PlGF, placental growth factor; PBS, phosphate-buffered saline; Ad Flt1, adenovirus expressing fms-like tyrosine kinase-1; Ad Fc, adenovirus-expressing control IgG2a Fc fragment; FC, fold change; Serpine2, serpin family E member 2; Id1, inhibitor of DNA-binding 1

Article Snippet: SMAD1 (D59D7) rabbit monoclonal antibody (#6944), phospho-SMAD1/5/9 (D5B10) rabbit monoclonal antibody (#13820), SMAD2 (D43B4) rabbit monoclonal antibody (#5339), phospho-SMAD2 (E8F3R) rabbit monoclonal antibody (#18338), SMAD3 (C67H9) rabbit monoclonal antibody (#9523), phospho-SMAD3 (C25A9) rabbit monoclonal antibody (# 9520), SMAD4 (D3R4N) rabbit monoclonal antibody (#46535), horseradish peroxidase (HRP)-linked anti-mouse IgG (#7076), and anti-rabbit IgG (#7074) were purchased from Cell Signaling Technology (Danvers, MA, USA); the mouse monoclonal antibody HLA-G (#11–499-C100) was purchased from EXBIO (Vestec, Czech Republic); CK7-specific rabbit polyclonal antibody (#17513-1-AP), BMP6 rabbit polyclonal antibody (#55421-1-AP), SERPINE2 rabbit polyclonal antibody (#11303-1-AP), and α-Tubulin mouse monoclonal antibody (#66031-1-Ig) were purchased from Proteintech (Wuhan, China); ID1 mouse monoclonal antibody (#sc-133104) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA); CD34 rabbit monoclonal antibody (#A19015) and α-SMA rabbit monoclonal antibody (#A2235) were obtained from ABclonal (Wuhan, China); Goat anti-Rabbit IgG secondary antibody, Alexa Fluor 594 (#A-11012) and goat anti-Mouse IgG secondary antibody, Alexa Fluor 488 (#A-11011) were purchased from Thermo Fisher (NY, USA).

Techniques: Expressing, Control, Western Blot, Clinical Proteomics, RNA Sequencing, Saline, Binding Assay

A Volcano plot of differentially expressed genes in SBC-2 cells with stable RASA4 knockdown, relative to those transfected with scramble shRNA. B qRT-PCR shows that SERPINE2 mRNA levels were significantly lower in DMS114 and SHP77 cells with stable over-expression of RASA4, compared to cells stably transfected with an empty vector. SERPINE2 mRNA levels were significantly higher in H446 and SBC-2 cells with stable knockdown of RASA4, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). C ELISA analysis shows that H446 and SBC-2 cells with RASA4 knockdown secreted more SERPINE2 protein to cell culture medium, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). D Immunofluorescence staining of SERPINE2 protein in SBC-2 and H446 cells with stable transfections of scramble shRNA and RASA4 shRNA. (scale bar = 10 μm for SBC-2 cells, scale bar = 20 μm for H446 cells). E Co-IP analysis of protein interaction between RASA4 and SERPINE2 in DMS114 cells with RASA4 stable over-expression. Cell lysates from DMS114 cells with stable transfection of FLAG-tagged RASA4 were immunoprecipitated with anti-FLAG antibody, followed by western blot with anti-SERPINE2 antibody. IgG was used as a negative control. F Western blot shows that the protein expression of SERPINE2 and EMT markers (N-cadherin, E-cadherin, Vinmintin, and snail) following RASA4 stable knockdown and overexpression in SBC-2 and DMS114 cells. G qRT-PCR analysis of stemness markers NANOG , OCT4 , SOX2, and MYC in SBC-2 and DMS114 cells with RASA4 stable knockdown and overexpression. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). H Sphere formation assay in SBC-2 and H446 cells with RASA4 stable knockdown, and in DMS114 and SHP77 cells with RASA4 stable overexpression. (scale bar = 50 μm).

Journal: Communications Biology

Article Title: Integrated methylome analysis identifies an epigenetically silenced tumor suppressor RASA4 in small cell lung cancer

doi: 10.1038/s42003-025-09440-7

Figure Lengend Snippet: A Volcano plot of differentially expressed genes in SBC-2 cells with stable RASA4 knockdown, relative to those transfected with scramble shRNA. B qRT-PCR shows that SERPINE2 mRNA levels were significantly lower in DMS114 and SHP77 cells with stable over-expression of RASA4, compared to cells stably transfected with an empty vector. SERPINE2 mRNA levels were significantly higher in H446 and SBC-2 cells with stable knockdown of RASA4, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). C ELISA analysis shows that H446 and SBC-2 cells with RASA4 knockdown secreted more SERPINE2 protein to cell culture medium, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). D Immunofluorescence staining of SERPINE2 protein in SBC-2 and H446 cells with stable transfections of scramble shRNA and RASA4 shRNA. (scale bar = 10 μm for SBC-2 cells, scale bar = 20 μm for H446 cells). E Co-IP analysis of protein interaction between RASA4 and SERPINE2 in DMS114 cells with RASA4 stable over-expression. Cell lysates from DMS114 cells with stable transfection of FLAG-tagged RASA4 were immunoprecipitated with anti-FLAG antibody, followed by western blot with anti-SERPINE2 antibody. IgG was used as a negative control. F Western blot shows that the protein expression of SERPINE2 and EMT markers (N-cadherin, E-cadherin, Vinmintin, and snail) following RASA4 stable knockdown and overexpression in SBC-2 and DMS114 cells. G qRT-PCR analysis of stemness markers NANOG , OCT4 , SOX2, and MYC in SBC-2 and DMS114 cells with RASA4 stable knockdown and overexpression. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). H Sphere formation assay in SBC-2 and H446 cells with RASA4 stable knockdown, and in DMS114 and SHP77 cells with RASA4 stable overexpression. (scale bar = 50 μm).

Article Snippet: SERPINE2 concentration in cell culture medium was quantified using the Human Glia-Derived Nexin (SERPINE2) ELISA Kit (#CSB-EL021082HU, Cusabio, Wuhan, China) following the manufacturer’s protocol.

Techniques: Knockdown, Transfection, shRNA, Quantitative RT-PCR, Over Expression, Stable Transfection, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Cell Culture, Immunofluorescence, Staining, Co-Immunoprecipitation Assay, Immunoprecipitation, Western Blot, Negative Control, Expressing, Tube Formation Assay

A Representative images of H&E staining in tumor and adjacent normal tissues from FFPE specimens of three surgical SCLC patients. B IHC staining of RASA4 in tumor and adjacent normal tissues from FFPE specimens of the same three SCLC patients. The results show significantly reduced RASA4 expression in the tumor tissues compared to the adjacent normal tissues. C IHC staining of SERPINE2 in tumor and adjacent normal tissues from FFPE specimens of the same three SCLC patient samples. The results show significantly increased SERPINE2 expression in the tumor tissues compared to the adjacent normal tissues. (scale bar = 20 μm for 40×, scale bar = 100 μm for 8×) ( D ) IHC staining shows that SERPINE2 expression is elevated in the invasive front of the SCLC tumor. (scale bar = 40 μm for 20×, scale bar = 200 μm for 5×) ( E ) IHC scores of RASA4 and SERPINE2 in tumor and adjacent normal tissues from 29 SCLC patients who underwent surgery ( n = 29, Mann–Whitney test). F Statistical analysis indicates the negative association between RASA4 and SERPINE2 expression in SCLC tumor and adjacent normal tissues ( n = 29, Chi-square test for R × C contingency tables). G Kaplan–Meier survival analysis of 29 SCLC patients who underwent surgery, stratified by RASA4 IHC scores in tumor tissues. The results show that SCLC patients with moderate scores of RASA4 expression have significantly better survival compared to those with weak scores. The statistical significance of the difference in survival was determined by the log-rank test.

Journal: Communications Biology

Article Title: Integrated methylome analysis identifies an epigenetically silenced tumor suppressor RASA4 in small cell lung cancer

doi: 10.1038/s42003-025-09440-7

Figure Lengend Snippet: A Representative images of H&E staining in tumor and adjacent normal tissues from FFPE specimens of three surgical SCLC patients. B IHC staining of RASA4 in tumor and adjacent normal tissues from FFPE specimens of the same three SCLC patients. The results show significantly reduced RASA4 expression in the tumor tissues compared to the adjacent normal tissues. C IHC staining of SERPINE2 in tumor and adjacent normal tissues from FFPE specimens of the same three SCLC patient samples. The results show significantly increased SERPINE2 expression in the tumor tissues compared to the adjacent normal tissues. (scale bar = 20 μm for 40×, scale bar = 100 μm for 8×) ( D ) IHC staining shows that SERPINE2 expression is elevated in the invasive front of the SCLC tumor. (scale bar = 40 μm for 20×, scale bar = 200 μm for 5×) ( E ) IHC scores of RASA4 and SERPINE2 in tumor and adjacent normal tissues from 29 SCLC patients who underwent surgery ( n = 29, Mann–Whitney test). F Statistical analysis indicates the negative association between RASA4 and SERPINE2 expression in SCLC tumor and adjacent normal tissues ( n = 29, Chi-square test for R × C contingency tables). G Kaplan–Meier survival analysis of 29 SCLC patients who underwent surgery, stratified by RASA4 IHC scores in tumor tissues. The results show that SCLC patients with moderate scores of RASA4 expression have significantly better survival compared to those with weak scores. The statistical significance of the difference in survival was determined by the log-rank test.

Article Snippet: SERPINE2 concentration in cell culture medium was quantified using the Human Glia-Derived Nexin (SERPINE2) ELISA Kit (#CSB-EL021082HU, Cusabio, Wuhan, China) following the manufacturer’s protocol.

Techniques: Staining, Immunohistochemistry, Expressing, MANN-WHITNEY

A Volcano plot of differentially expressed genes in SBC-2 cells with stable RASA4 knockdown, relative to those transfected with scramble shRNA. B qRT-PCR shows that SERPINE2 mRNA levels were significantly lower in DMS114 and SHP77 cells with stable over-expression of RASA4, compared to cells stably transfected with an empty vector. SERPINE2 mRNA levels were significantly higher in H446 and SBC-2 cells with stable knockdown of RASA4, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). C ELISA analysis shows that H446 and SBC-2 cells with RASA4 knockdown secreted more SERPINE2 protein to cell culture medium, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). D Immunofluorescence staining of SERPINE2 protein in SBC-2 and H446 cells with stable transfections of scramble shRNA and RASA4 shRNA. (scale bar = 10 μm for SBC-2 cells, scale bar = 20 μm for H446 cells). E Co-IP analysis of protein interaction between RASA4 and SERPINE2 in DMS114 cells with RASA4 stable over-expression. Cell lysates from DMS114 cells with stable transfection of FLAG-tagged RASA4 were immunoprecipitated with anti-FLAG antibody, followed by western blot with anti-SERPINE2 antibody. IgG was used as a negative control. F Western blot shows that the protein expression of SERPINE2 and EMT markers (N-cadherin, E-cadherin, Vinmintin, and snail) following RASA4 stable knockdown and overexpression in SBC-2 and DMS114 cells. G qRT-PCR analysis of stemness markers NANOG , OCT4 , SOX2, and MYC in SBC-2 and DMS114 cells with RASA4 stable knockdown and overexpression. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). H Sphere formation assay in SBC-2 and H446 cells with RASA4 stable knockdown, and in DMS114 and SHP77 cells with RASA4 stable overexpression. (scale bar = 50 μm).

Journal: Communications Biology

Article Title: Integrated methylome analysis identifies an epigenetically silenced tumor suppressor RASA4 in small cell lung cancer

doi: 10.1038/s42003-025-09440-7

Figure Lengend Snippet: A Volcano plot of differentially expressed genes in SBC-2 cells with stable RASA4 knockdown, relative to those transfected with scramble shRNA. B qRT-PCR shows that SERPINE2 mRNA levels were significantly lower in DMS114 and SHP77 cells with stable over-expression of RASA4, compared to cells stably transfected with an empty vector. SERPINE2 mRNA levels were significantly higher in H446 and SBC-2 cells with stable knockdown of RASA4, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). C ELISA analysis shows that H446 and SBC-2 cells with RASA4 knockdown secreted more SERPINE2 protein to cell culture medium, compared to cells stably transfected with scramble shRNA. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). D Immunofluorescence staining of SERPINE2 protein in SBC-2 and H446 cells with stable transfections of scramble shRNA and RASA4 shRNA. (scale bar = 10 μm for SBC-2 cells, scale bar = 20 μm for H446 cells). E Co-IP analysis of protein interaction between RASA4 and SERPINE2 in DMS114 cells with RASA4 stable over-expression. Cell lysates from DMS114 cells with stable transfection of FLAG-tagged RASA4 were immunoprecipitated with anti-FLAG antibody, followed by western blot with anti-SERPINE2 antibody. IgG was used as a negative control. F Western blot shows that the protein expression of SERPINE2 and EMT markers (N-cadherin, E-cadherin, Vinmintin, and snail) following RASA4 stable knockdown and overexpression in SBC-2 and DMS114 cells. G qRT-PCR analysis of stemness markers NANOG , OCT4 , SOX2, and MYC in SBC-2 and DMS114 cells with RASA4 stable knockdown and overexpression. Data are shown as mean ± SD from three independent biological replicates ( n = 3, unpaired Student’s t -test). H Sphere formation assay in SBC-2 and H446 cells with RASA4 stable knockdown, and in DMS114 and SHP77 cells with RASA4 stable overexpression. (scale bar = 50 μm).

Article Snippet: SERPINE2 concentration in cell culture medium was quantified using the Human Glia-Derived Nexin (SERPINE2) ELISA Kit (#CSB-EL021082HU, Cusabio, Wuhan, China) following the manufacturer’s protocol.

Techniques: Knockdown, Transfection, shRNA, Quantitative RT-PCR, Over Expression, Stable Transfection, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Cell Culture, Immunofluorescence, Staining, Co-Immunoprecipitation Assay, Immunoprecipitation, Western Blot, Negative Control, Expressing, Tube Formation Assay

A Representative images of H&E staining in tumor and adjacent normal tissues from FFPE specimens of three surgical SCLC patients. B IHC staining of RASA4 in tumor and adjacent normal tissues from FFPE specimens of the same three SCLC patients. The results show significantly reduced RASA4 expression in the tumor tissues compared to the adjacent normal tissues. C IHC staining of SERPINE2 in tumor and adjacent normal tissues from FFPE specimens of the same three SCLC patient samples. The results show significantly increased SERPINE2 expression in the tumor tissues compared to the adjacent normal tissues. (scale bar = 20 μm for 40×, scale bar = 100 μm for 8×) ( D ) IHC staining shows that SERPINE2 expression is elevated in the invasive front of the SCLC tumor. (scale bar = 40 μm for 20×, scale bar = 200 μm for 5×) ( E ) IHC scores of RASA4 and SERPINE2 in tumor and adjacent normal tissues from 29 SCLC patients who underwent surgery ( n = 29, Mann–Whitney test). F Statistical analysis indicates the negative association between RASA4 and SERPINE2 expression in SCLC tumor and adjacent normal tissues ( n = 29, Chi-square test for R × C contingency tables). G Kaplan–Meier survival analysis of 29 SCLC patients who underwent surgery, stratified by RASA4 IHC scores in tumor tissues. The results show that SCLC patients with moderate scores of RASA4 expression have significantly better survival compared to those with weak scores. The statistical significance of the difference in survival was determined by the log-rank test.

Journal: Communications Biology

Article Title: Integrated methylome analysis identifies an epigenetically silenced tumor suppressor RASA4 in small cell lung cancer

doi: 10.1038/s42003-025-09440-7

Figure Lengend Snippet: A Representative images of H&E staining in tumor and adjacent normal tissues from FFPE specimens of three surgical SCLC patients. B IHC staining of RASA4 in tumor and adjacent normal tissues from FFPE specimens of the same three SCLC patients. The results show significantly reduced RASA4 expression in the tumor tissues compared to the adjacent normal tissues. C IHC staining of SERPINE2 in tumor and adjacent normal tissues from FFPE specimens of the same three SCLC patient samples. The results show significantly increased SERPINE2 expression in the tumor tissues compared to the adjacent normal tissues. (scale bar = 20 μm for 40×, scale bar = 100 μm for 8×) ( D ) IHC staining shows that SERPINE2 expression is elevated in the invasive front of the SCLC tumor. (scale bar = 40 μm for 20×, scale bar = 200 μm for 5×) ( E ) IHC scores of RASA4 and SERPINE2 in tumor and adjacent normal tissues from 29 SCLC patients who underwent surgery ( n = 29, Mann–Whitney test). F Statistical analysis indicates the negative association between RASA4 and SERPINE2 expression in SCLC tumor and adjacent normal tissues ( n = 29, Chi-square test for R × C contingency tables). G Kaplan–Meier survival analysis of 29 SCLC patients who underwent surgery, stratified by RASA4 IHC scores in tumor tissues. The results show that SCLC patients with moderate scores of RASA4 expression have significantly better survival compared to those with weak scores. The statistical significance of the difference in survival was determined by the log-rank test.

Article Snippet: SERPINE2 concentration in cell culture medium was quantified using the Human Glia-Derived Nexin (SERPINE2) ELISA Kit (#CSB-EL021082HU, Cusabio, Wuhan, China) following the manufacturer’s protocol.

Techniques: Staining, Immunohistochemistry, Expressing, MANN-WHITNEY

SERPINE2 expression is significantly upregulated in COAD. (A) Box plot of SERPINE family genes mRNA expression.in COAD(p < 0.05). (B) Box plot of SERPINE1 and SERPINE2 protein expression in COAD(p < 0.05). (C) Box plot of SERPINE2 mRNA expression among groups of different tumor grades in COAD (p < 0.05).

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 expression is significantly upregulated in COAD. (A) Box plot of SERPINE family genes mRNA expression.in COAD(p < 0.05). (B) Box plot of SERPINE1 and SERPINE2 protein expression in COAD(p < 0.05). (C) Box plot of SERPINE2 mRNA expression among groups of different tumor grades in COAD (p < 0.05).

Article Snippet: Concentrations of SERPINE2 (human) in the culture supernatant of treated cells were measured with the use of a commercially available kit (CUSABIO).Seed HCT-8 cells in a 100-mm culture dish at a density of 1 × 106 cells in complete medium (RPMI-1640,+10%FBS).At 60–80% confluency, perform siRNA transfection using [Lipofectamine 3000].

Techniques: Expressing

SERPINE2 expression correlates with tumor immunoregulation, division and proliferation. (A) Volcano map of SERPINE2 co-expressed genes in COAD. (B, C) GO and KEGG enrichment analysis of SERPINE2 co-expressed genes obtained from the cBioPortal, the terms p and q < 0.05 were considered to be significantly enriched. Only 20 leading gene sets are displayed in the plot.

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 expression correlates with tumor immunoregulation, division and proliferation. (A) Volcano map of SERPINE2 co-expressed genes in COAD. (B, C) GO and KEGG enrichment analysis of SERPINE2 co-expressed genes obtained from the cBioPortal, the terms p and q < 0.05 were considered to be significantly enriched. Only 20 leading gene sets are displayed in the plot.

Article Snippet: Concentrations of SERPINE2 (human) in the culture supernatant of treated cells were measured with the use of a commercially available kit (CUSABIO).Seed HCT-8 cells in a 100-mm culture dish at a density of 1 × 106 cells in complete medium (RPMI-1640,+10%FBS).At 60–80% confluency, perform siRNA transfection using [Lipofectamine 3000].

Techniques: Expressing

SERPINE2 is positively correlated with M2 macrophage infiltration in COAD. (A) Scatter plot showing the correlation of 6 different immune cell types and SERPINE2 expression (p < 0.05). The black line in each plot is a fitted linear model indicating the proportion of tropism of the immune cell along with SERPINE2 expression, and the Pearson coefficient was used for the correlation test. (B) Scatter plot showing the correlation of CD68+ Marcophage and SERPINE2 expression (p < 0.05). C.Scatter plot showing the correlation of M1/M2 macrophage and SERPINE2 expression (p < 0.05). The blue line in each plot is a fitted linear model indicating the proportion of tropism of the immune cell along with SERPINE2 expression, and the Pearson coefficient was used for the correlation test. (p < 0.05).

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 is positively correlated with M2 macrophage infiltration in COAD. (A) Scatter plot showing the correlation of 6 different immune cell types and SERPINE2 expression (p < 0.05). The black line in each plot is a fitted linear model indicating the proportion of tropism of the immune cell along with SERPINE2 expression, and the Pearson coefficient was used for the correlation test. (B) Scatter plot showing the correlation of CD68+ Marcophage and SERPINE2 expression (p < 0.05). C.Scatter plot showing the correlation of M1/M2 macrophage and SERPINE2 expression (p < 0.05). The blue line in each plot is a fitted linear model indicating the proportion of tropism of the immune cell along with SERPINE2 expression, and the Pearson coefficient was used for the correlation test. (p < 0.05).

Article Snippet: Concentrations of SERPINE2 (human) in the culture supernatant of treated cells were measured with the use of a commercially available kit (CUSABIO).Seed HCT-8 cells in a 100-mm culture dish at a density of 1 × 106 cells in complete medium (RPMI-1640,+10%FBS).At 60–80% confluency, perform siRNA transfection using [Lipofectamine 3000].

Techniques: Expressing

SERPINE2 is associated with infiltration of CD68 macrophage in COAD patients. (A) The expression of SEPRINE2 in patient tissues was detected by IHC. Adjacent tissue not expressing SERPINE2; tumor tissue strongly expressing SERPINE2. (B) Scatterplot showing the correlation between SERPINE2 and CD68 expression (p < 0.05). The blue line in each plot is a fitted linear model indicating the relationship between SERPINE2 and CD68 expression. (C) The expression of SERPINE2 and CD68 in patient tissues were detected by IHC. High levels of SERPINE2 expression are associated with increased expression of CD68. (* p<0.05, **p<0.01, *** p<0.001).

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: SERPINE2 is associated with infiltration of CD68 macrophage in COAD patients. (A) The expression of SEPRINE2 in patient tissues was detected by IHC. Adjacent tissue not expressing SERPINE2; tumor tissue strongly expressing SERPINE2. (B) Scatterplot showing the correlation between SERPINE2 and CD68 expression (p < 0.05). The blue line in each plot is a fitted linear model indicating the relationship between SERPINE2 and CD68 expression. (C) The expression of SERPINE2 and CD68 in patient tissues were detected by IHC. High levels of SERPINE2 expression are associated with increased expression of CD68. (* p<0.05, **p<0.01, *** p<0.001).

Article Snippet: Concentrations of SERPINE2 (human) in the culture supernatant of treated cells were measured with the use of a commercially available kit (CUSABIO).Seed HCT-8 cells in a 100-mm culture dish at a density of 1 × 106 cells in complete medium (RPMI-1640,+10%FBS).At 60–80% confluency, perform siRNA transfection using [Lipofectamine 3000].

Techniques: Expressing

The external secretion of SERPINE2 from colorectal cancer cells promotes macrophage polarization to accelerate the development of colorectal cancer. (A) Schema diagram showing that THP-1 cells were treated with PMA, followed by the treatment of SERPINE2 recombinant protein as indicated treatments to obtain activated macrophages. Then tumor cells cocultured with these macrophages. were used to subsequent experiments. (B, C) RT-qPCR and Westen blot displayed SERPINE2 mRNA and Protein expression were upregulated in colorectal cancer cells. (D) The secretion of SERPINE2 in colorectal cancer cells was detected by ELISA. (E) The effect of SERPINE2 recombinant protein on macrophage polarization. (F) RT-qPCR displayed conditioned medium (CM) derived from colorectal cancer cells promotes the upregulation of macrophage M2 markers, compared to normal Intestinal cells. (G) ELISA analysis demonstrated that SERPINE2 secretion was significantly reduced after SERPINE2 knockdown. (H) RT-qPCR displayed conditioned medium with SERPINE2 knockdown down-regulated macrophage M2 markers. (I, J) The conditioned medium from macrophages treated with SERPINE2 recombinant protein promoted cancer cell proliferation (measured by CCK-8 assay) and migration (assessed by Transwell assay. (*p<0.05, **p<0.01, ***p<0.001).

Journal: Frontiers in Oncology

Article Title: SerpinE2 promotes M2 polarization in macrophage to accelerate colorectal cancer progression

doi: 10.3389/fonc.2025.1585935

Figure Lengend Snippet: The external secretion of SERPINE2 from colorectal cancer cells promotes macrophage polarization to accelerate the development of colorectal cancer. (A) Schema diagram showing that THP-1 cells were treated with PMA, followed by the treatment of SERPINE2 recombinant protein as indicated treatments to obtain activated macrophages. Then tumor cells cocultured with these macrophages. were used to subsequent experiments. (B, C) RT-qPCR and Westen blot displayed SERPINE2 mRNA and Protein expression were upregulated in colorectal cancer cells. (D) The secretion of SERPINE2 in colorectal cancer cells was detected by ELISA. (E) The effect of SERPINE2 recombinant protein on macrophage polarization. (F) RT-qPCR displayed conditioned medium (CM) derived from colorectal cancer cells promotes the upregulation of macrophage M2 markers, compared to normal Intestinal cells. (G) ELISA analysis demonstrated that SERPINE2 secretion was significantly reduced after SERPINE2 knockdown. (H) RT-qPCR displayed conditioned medium with SERPINE2 knockdown down-regulated macrophage M2 markers. (I, J) The conditioned medium from macrophages treated with SERPINE2 recombinant protein promoted cancer cell proliferation (measured by CCK-8 assay) and migration (assessed by Transwell assay. (*p<0.05, **p<0.01, ***p<0.001).

Article Snippet: Concentrations of SERPINE2 (human) in the culture supernatant of treated cells were measured with the use of a commercially available kit (CUSABIO).Seed HCT-8 cells in a 100-mm culture dish at a density of 1 × 106 cells in complete medium (RPMI-1640,+10%FBS).At 60–80% confluency, perform siRNA transfection using [Lipofectamine 3000].

Techniques: Recombinant, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Derivative Assay, Knockdown, CCK-8 Assay, Migration, Transwell Assay

Antibody specificity and presence of the SERPINE2 protein in human uterine fluid . (A) Four hundred nanograms of recombinant human SERPINE2 was resolved on 10% SDS-PAGE and followed by Western blotting using anti-mouse SERPINE2 antiserum (lane 1), an anti-human SERPINE2 antibody (R&D) (lane2), or another anti-human SERPINE2 antibody (Abnova) (lane 3). (B) One hundred micrograms of the extract of endometrial curettage was analyzed by anti-mouse SERPINE2 antiserum (lanes 1 and 2) and an anti-human SERPINE2 antibody (R&D) (lanes 3 and 4). (C) Fifty micrograms of uterine fluid proteins collected from each individual patient ( n = 7) was Western-blotted using anti-mouse SERPINE2 antiserum (1:3000) (upper panel). EP, MP, and LP indicate early-, mid-, and late-proliferative phases, and ES and MS indicate early- and mid-secretory phases, respectively. The blot was also overexposed to clearly display the staining signal (lower panel).

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: SERPINE2, an inhibitor of plasminogen activators, is highly expressed in the human endometrium during the secretory phase

doi: 10.1186/1477-7827-9-38

Figure Lengend Snippet: Antibody specificity and presence of the SERPINE2 protein in human uterine fluid . (A) Four hundred nanograms of recombinant human SERPINE2 was resolved on 10% SDS-PAGE and followed by Western blotting using anti-mouse SERPINE2 antiserum (lane 1), an anti-human SERPINE2 antibody (R&D) (lane2), or another anti-human SERPINE2 antibody (Abnova) (lane 3). (B) One hundred micrograms of the extract of endometrial curettage was analyzed by anti-mouse SERPINE2 antiserum (lanes 1 and 2) and an anti-human SERPINE2 antibody (R&D) (lanes 3 and 4). (C) Fifty micrograms of uterine fluid proteins collected from each individual patient ( n = 7) was Western-blotted using anti-mouse SERPINE2 antiserum (1:3000) (upper panel). EP, MP, and LP indicate early-, mid-, and late-proliferative phases, and ES and MS indicate early- and mid-secretory phases, respectively. The blot was also overexposed to clearly display the staining signal (lower panel).

Article Snippet: Membranes were blocked with 10% (w/v) skim milk in phosphate-buffered saline (PBS) (blocking solution) for 2 h, and then incubated with our homemade anti-mouse SERPINE2 antiserum (1: 5000) [ , ], anti-human SERPINE2 antibody (1: 1000, catalog no. AF2980, R&D Systems, Minneapolis, MN, USA), or another anti-human SERPINE2 antibody (1: 1000, product no. H00005270-B01, Abnova) in blocking solution for 2 h at 37°C.

Techniques: Recombinant, SDS Page, Western Blot, Staining

Localization of SERPINE2 in the human uterus . Longitudinal sections of the early secretory phase uterus (n = 5) on the slide were incubated with anti-mouse SERPINE2 antiserum and then treated with biotin-conjugated goat-anti-rabbit IgG and HRP-conjugated streptavidin (brown). For contrast, specimens were further stained with hematoxylin (blue). The representative picture is shown. Magnified pictures of the luminal epithelium (A), glandular epithelium (B), and myometrium (C) are shown. Bar = 50 μm. bv, blood vessel; ge, glandular epithelium; le, luminal epithelium; m, muscle; s, stroma.

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: SERPINE2, an inhibitor of plasminogen activators, is highly expressed in the human endometrium during the secretory phase

doi: 10.1186/1477-7827-9-38

Figure Lengend Snippet: Localization of SERPINE2 in the human uterus . Longitudinal sections of the early secretory phase uterus (n = 5) on the slide were incubated with anti-mouse SERPINE2 antiserum and then treated with biotin-conjugated goat-anti-rabbit IgG and HRP-conjugated streptavidin (brown). For contrast, specimens were further stained with hematoxylin (blue). The representative picture is shown. Magnified pictures of the luminal epithelium (A), glandular epithelium (B), and myometrium (C) are shown. Bar = 50 μm. bv, blood vessel; ge, glandular epithelium; le, luminal epithelium; m, muscle; s, stroma.

Article Snippet: Membranes were blocked with 10% (w/v) skim milk in phosphate-buffered saline (PBS) (blocking solution) for 2 h, and then incubated with our homemade anti-mouse SERPINE2 antiserum (1: 5000) [ , ], anti-human SERPINE2 antibody (1: 1000, catalog no. AF2980, R&D Systems, Minneapolis, MN, USA), or another anti-human SERPINE2 antibody (1: 1000, product no. H00005270-B01, Abnova) in blocking solution for 2 h at 37°C.

Techniques: Incubation, Staining

Glandular epithelial expression of the SERPINE2 protein in the endometrium during the menstrual cycle . Sections prepared from endometrial curettage during early-proliferative (EP), mid-proliferative (MP), late-proliferative (LP), early-secretory (ES), mid-secretory (MS), and late-secretory (LS) phases were incubated with anti-mouse SERPINE2 antiserum and then treated as described in Figure 2. Bar = 50 μm. bv, blood vessel; ge, glandular epithelium; s, stroma.

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: SERPINE2, an inhibitor of plasminogen activators, is highly expressed in the human endometrium during the secretory phase

doi: 10.1186/1477-7827-9-38

Figure Lengend Snippet: Glandular epithelial expression of the SERPINE2 protein in the endometrium during the menstrual cycle . Sections prepared from endometrial curettage during early-proliferative (EP), mid-proliferative (MP), late-proliferative (LP), early-secretory (ES), mid-secretory (MS), and late-secretory (LS) phases were incubated with anti-mouse SERPINE2 antiserum and then treated as described in Figure 2. Bar = 50 μm. bv, blood vessel; ge, glandular epithelium; s, stroma.

Article Snippet: Membranes were blocked with 10% (w/v) skim milk in phosphate-buffered saline (PBS) (blocking solution) for 2 h, and then incubated with our homemade anti-mouse SERPINE2 antiserum (1: 5000) [ , ], anti-human SERPINE2 antibody (1: 1000, catalog no. AF2980, R&D Systems, Minneapolis, MN, USA), or another anti-human SERPINE2 antibody (1: 1000, product no. H00005270-B01, Abnova) in blocking solution for 2 h at 37°C.

Techniques: Expressing, Incubation

Quantification of SERPINE2 protein expression levels in endometrial glands . Representative samples were analyzed by automated cell acquisition and quantification software (A). The expression signal of a respective glandular gland was quantified using HistoQuest software and is presented as a scattergram. Each spot on the scattergram stands for the intensity of one cell (B). The relative SERPINE2 protein expression levels in patients' glandular glands at various sub-phases of the menstrual cycle are shown as bar diagrams (C). Differences are significant among patients at various groups (χ = 69.32, p < 0.0001).

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: SERPINE2, an inhibitor of plasminogen activators, is highly expressed in the human endometrium during the secretory phase

doi: 10.1186/1477-7827-9-38

Figure Lengend Snippet: Quantification of SERPINE2 protein expression levels in endometrial glands . Representative samples were analyzed by automated cell acquisition and quantification software (A). The expression signal of a respective glandular gland was quantified using HistoQuest software and is presented as a scattergram. Each spot on the scattergram stands for the intensity of one cell (B). The relative SERPINE2 protein expression levels in patients' glandular glands at various sub-phases of the menstrual cycle are shown as bar diagrams (C). Differences are significant among patients at various groups (χ = 69.32, p < 0.0001).

Article Snippet: Membranes were blocked with 10% (w/v) skim milk in phosphate-buffered saline (PBS) (blocking solution) for 2 h, and then incubated with our homemade anti-mouse SERPINE2 antiserum (1: 5000) [ , ], anti-human SERPINE2 antibody (1: 1000, catalog no. AF2980, R&D Systems, Minneapolis, MN, USA), or another anti-human SERPINE2 antibody (1: 1000, product no. H00005270-B01, Abnova) in blocking solution for 2 h at 37°C.

Techniques: Expressing, Software