Journal: bioRxiv

Article Title: Genetically diverse mouse models of SARS-CoV-2 infection model clinical variation and cytokine responses in COVID-19

doi: 10.1101/2021.09.17.460664

Figure Lengend Snippet: Mice were infected intranasally with 10 3 PFU of SARS-CoV-2 and monitored for survival and weight loss. F1 founder strains were classified as: a , sensitive with 50% or greater mortality and no difference between males and females, b , resistant with 35% or less mortality, or c , sensitive with a sex bias as having statistically significant difference between sensitivity of male and female mice. Mice without evidence of seroconversion at the end of the study were removed from analysis (N=6-8/per sex).

Article Snippet: SARS-CoV-2 spike protein-specific IgG was measured using SARS-CoV2 spike protein serological IgG ELISA kit (Cell Signaling Technology) per manufacturer’s instructions.

Techniques: Infection

Journal: bioRxiv

Article Title: Genetically diverse mouse models of SARS-CoV-2 infection model clinical variation and cytokine responses in COVID-19

doi: 10.1101/2021.09.17.460664

Figure Lengend Snippet: a , Virus titers (PFU/g tissue) in lung and brain of male and female mice were measured at 3 and 6 dpi. b , RNAscope of SARS-CoV-2 genome predominantly localizing to pneumocytes in areas of lung inflammation. Serial sections demonstrate pathology (upper) and viral RNA distribution (lower; Red). Scale bars represent 100μm. c , Examples of pathology scoring used in this study. Scale bars represent 500μm. d , Lung pathology scores for CC Founder F1 males and females at 3 and 6 dpi. N = 6/time point/sex. ND, not determined.

Article Snippet: SARS-CoV-2 spike protein-specific IgG was measured using SARS-CoV2 spike protein serological IgG ELISA kit (Cell Signaling Technology) per manufacturer’s instructions.

Techniques: Virus, RNAscope

Journal: bioRxiv

Article Title: Genetically diverse mouse models of SARS-CoV-2 infection model clinical variation and cytokine responses in COVID-19

doi: 10.1101/2021.09.17.460664

Figure Lengend Snippet: a , Small focal areas of inflammation were most commonly observed (circled). Scale bar = 1000μm. b , increased magnification of a. demonstrating perivascular cuffing, gliosis, and evidence of necrotic cells in areas of focal inflammation. Scale bar = 200μm. c , Example of a pronounced lymphocytic inflammatory response with perivascular cuffing and increased cellularity (arrowheads). Scale bar = 100μm. d , Microthrombus occluding cerebral capillaries and hemorrhage (arrow) observed in CASTxK18-hACE2. Scale bar = 100μm. e , Example of extensive hemorrhage in the brain of CASTxK18-hACE2 F1 progeny associated with extensive virus replication as demonstrated in f. Scale bar = 1000μm. f , serial section relative to e. showing extensive SARS-CoV-2 genome by RNAscope. Scale bar = 1000μm. g , Incidence of encephalitis for CC Founder F1 males and females at 3 and 6 dpi. ND, not determined.

Article Snippet: SARS-CoV-2 spike protein-specific IgG was measured using SARS-CoV2 spike protein serological IgG ELISA kit (Cell Signaling Technology) per manufacturer’s instructions.

Techniques: Virus, RNAscope

Journal: STAR Protocols

Article Title: Protocol to characterize immune cell subpopulations in cerebrospinal fluid of patients with neuroinflammatory diseases using mass cytometry

doi: 10.1016/j.xpro.2024.103038

Figure Lengend Snippet:

Article Snippet: 5 mL serological pipettes , Eppendorf , Cat#0030127714.

Techniques: Recombinant, Labeling, Blocking Assay, Software, Cytometry, Flow Cytometry, Single-cell Analysis, Gene Expression