Journal: Molecular Medicine Reports

Article Title: Tea seed saponin‑reduced extract ameliorates palmitic acid‑induced insulin resistance in HepG2 cells

doi: 10.3892/mmr.2023.13149

Figure Lengend Snippet: Effects of TSSRE on GSK-3β phosphorylation in HepG2 cells. HepG2 cells were treated with normal-(5.5 mM) or high-concentration (30 mM) glucose plus 0.25 mM PA in the absence or presence of TSSRE for 24 h and subsequently treated with insulin (100 nM) for 30 min. *P<0.05. TSSRE, tea seed saponin-reduced extract; GSK-3β, glycogen synthase kinase 3β; PA, palmitic acid; p-, phosphorylated.

Article Snippet: After blocking, these membranes were incubated with the following primary antibodies at 4°C overnight: Phosphorylated (p-)p38 (cat. no. 4511; 1:1,000), p38 (cat. no. 8690; 1:1,000), p-ERK (cat. no. 4370; 1:1,000), ERK (cat. no. 4695; 1:1,000), p-JNK (cat. no. 4668; 1:1,000), JNK (cat. no. 9252; 1:1,000), p-Akt (cat. no. 9271; 1:1,000), Akt (cat. no. 9272; 1:1,000), p-IRS1 (cat. no. 3070; 1:1,000), IRS1 (all from Cell Signaling Technology, Inc.; cat. no. 2382; 1:1,000), phosphoenolpyruvate carboxykinase (PEPCK; cat. no. E-AB-11396; 1:1,000), p-GSK-3β (cat. no. E-AB-20886; 1:1,000), GSK-3β (cat. no. E-AB-31629; 1:1,000), glucose transporter (GLUT) 4 (all from Elabscience Biotechnology, Inc.; cat. no. E-AB-30268; 1:1,000), GLUT2 (Proteintech Group, Inc.; cat. no. 20436-1-AP; 1:1,000), p-FOXO1 (cat. no. AF3417; 1:1,000), FOXO1 (both from Affinity Biosciences; cat. no. AF6416; 1:1,000), and β-actin (iReal Biotechnology, Inc.; cat. no. IR2-7; 1:1,000).

Techniques: Phospho-proteomics, Concentration Assay

Journal: Biomedicines

Article Title: Inhibition of AKT/GSK3β/CREB Pathway Improves the Responsiveness to AMPA Receptor Antagonists by Regulating GRIA1 Surface Expression in Chronic Epilepsy Rats

doi: 10.3390/biomedicines9040425

Figure Lengend Snippet: Primary antibodies used in the present study.

Article Snippet: p-GSK3β S9 , Rabbit , Biorbyt (orb14745) , 1:1000 (WB).

Techniques:

Journal: Biomedicines

Article Title: Inhibition of AKT/GSK3β/CREB Pathway Improves the Responsiveness to AMPA Receptor Antagonists by Regulating GRIA1 Surface Expression in Chronic Epilepsy Rats

doi: 10.3390/biomedicines9040425

Figure Lengend Snippet: The effects of perampanel (PER) and GYKI 52466 (GYKI) on protein interacting with C kinase 1 (PICK1) expression and glycogen synthase kinase 3β (GSK3β) expression and its phosphorylation in chronic epilepsy rats. ( A , B ) Representative images for Western blot of PICK1 expression ( A ) and quantifications of PICK1 expression ( B ) in the hippocampal tissues. ( C – F ) Representative images for Western blot of GSK3β expression and its phosphorylation ( C ) and quantifications of GSK3β ( D ), p-GSK3β S9 ( E ), and p-GSK3β S9/GSK3β ratio ( F ) in the hippocampal tissues. Open circles indicate each individual value. Horizontal bars indicate mean value. Error bars indicate SEM ( *, # p < 0.05 vs. control (Cont) and vehicle (Veh)-treated animals, respectively; one-way ANOVA with post hoc Bonferroni’s multiple comparison).

Article Snippet: p-GSK3β S9 , Rabbit , Biorbyt (orb14745) , 1:1000 (WB).

Techniques: Expressing, Phospho-proteomics, Western Blot, Control, Comparison

Journal: Biomedicines

Article Title: Inhibition of AKT/GSK3β/CREB Pathway Improves the Responsiveness to AMPA Receptor Antagonists by Regulating GRIA1 Surface Expression in Chronic Epilepsy Rats

doi: 10.3390/biomedicines9040425

Figure Lengend Snippet: The effects of 3CAI co-treatment with perampanel (PER) and GYKI 52466 (GYKI) on expression levels of GSK3β, CREB, and PICK1 and the phosphorylation levels of GSK3β and CREB in non-responders. ( A ) Representative images for Western blot of total GSK3β, CREB, and PICK1 levels and the phosphorylation levels of GSK3β and CREB. ( B – H ) Quantifications of GSK3β ( B ), p-GSK3β S9 ( C ), p-GSK3β S9/GSK3β ratio ( D ), CREB ( E ), p-CREB S133 ( F ), p-CREB S133/CREB ratio ( G ), and PICK1 ( H ) in the hippocampal tissues. Open circles indicate each individual value. Horizontal bars indicate mean value. Error bars indicate SEM ( *, # p < 0.05 vs. control (Cont) and vehicle (Veh)-treated animals, respectively; one-way ANOVA with post hoc Bonferroni’s multiple comparison).

Article Snippet: p-GSK3β S9 , Rabbit , Biorbyt (orb14745) , 1:1000 (WB).

Techniques: Expressing, Phospho-proteomics, Western Blot, Control, Comparison