Journal: Animal Reproduction

Article Title: Long-read and short-read RNA-seq reveal the transcriptional regulation characteristics of PICK1 in Baoshan pig testis

doi: 10.1590/1984-3143-AR2024-0047

Figure Lengend Snippet: Gene structure of PICK1 . (A) Chromosome location, exon, and intron abundance based on transcriptome sequencing; (B) PICK1 gene coding sequence and amino acid sequence.

Article Snippet: Long-read sequencing data were obtained from Wuhan GrandOmics Co., Ltd. China, while short-read sequencing data were obtained from Tianjin Novogene Co., Ltd., China.

Techniques: Sequencing

Journal: Royal Society Open Science

Article Title: The unexpected loss of the ‘hunger hormone’ ghrelin in true passerines: a game changer in migration physiology

doi: 10.1098/rsos.242107

Figure Lengend Snippet: GHS-R abundance was measured using an enzyme immunoassay in plasma of the common quail and the garden warbler. Boxplot of the concentration values for GHS-R detected in plasma samples of the common quail and the garden warbler. Given the high homology of the GHS-R sequence within avian species, the chicken anti-GHS-R antibody was sensitive and cross-reacts with both species.

Article Snippet: Furthermore, our blast search also included genomes generated with Oxford Nanopore Technologies’ long-read sequencing data (e.g. GCA_004360235.1), which show no GC-bias [ ], and genomes generated with Pacific Biosciences (PacBio) long-read sequencing data (e.g. GCA_009819655.1), which show less GC-bias than Illumina-based sequencing methods (e.g. [ ]); however, negative effects of GC content have been found in [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Concentration Assay, Sequencing