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Image Search Results
Journal: Scientific Reports
Article Title: The NOP-1 peptide derived from the central regulator of ethylene signaling EIN2 delays floral senescence in cut flowers
doi: 10.1038/s41598-018-37571-x
Figure Lengend Snippet: Molecular binding studies of carnation ethylene receptor DcETR1. ( A ) Microscale Thermophoresis (MST) binding studies using recombinant carnation ethylene receptor DcETR1 and AtEIN2. Titration of unlabeled AtEIN2 to labeled DcETR1 (•) revealed binding of both proteins at a dissociation constant (K D ) of 135 nM ± 0.35 nM, indicating a tight interaction between DcETR1 and AtEIN2. ( B ) MST binding studies on DcETR1 and NOP-1. Titration of unlabeled NOP-1 to labeled DcETR1 (•) resulted in a K D of 3.49 µM ± 0.55 µM. Asterisks (*) indicate the labeling of DcETR1 with AlexaFluor488-NHS. Negative controls using chemically denatured DcETR1 shows no interaction with the EIN2 protein or the NOP-1 peptide (•).
Article Snippet: According to the published sequence (Carnation DB: Dca62022.1),
Techniques: Binding Assay, Microscale Thermophoresis, Recombinant, Titration, Labeling