Journal: The Pharmacogenomics Journal

Article Title: A TOMM40 variable-length polymorphism predicts the age of late-onset Alzheimer's disease

doi: 10.1038/tpj.2009.69

Figure Lengend Snippet: Schematic overview of the TOMM40-APOE locus. ( a ) Genomic locations of the TOMM40 and APOE genes on chromosome 19 between 50 084 480 and 50 107 480 bp. The regions subjected to primary sequencing and phylogenetic analysis for the exploratory (R1) (23 kb) and confirmatory (R2) (10 kb) studies are highlighted on the genomic map (NCBI Build 36.3). ( b ) Distribution of SNP and insertion/deletion polymorphisms are shown on the gene structure of TOMM40 covered in the region that was subjected to primary sequencing. The region covers exons 6–10 and all associated intronic regions. The variable poly-T repeat (rs10524523) that is significantly associated with LOAD age of onset is depicted with the square labeled ‘P'.

Article Snippet: A proprietary sequencing analysis program called ‘Agent' (developed by Celera, Alameda, CA, USA) was used to align sequencing reads to the appropriate reference sequence, and produce ‘contigs' associated with each clone.

Techniques: Sequencing, Labeling

Journal: Biochimie Open

Article Title: Identification of a nicotinamide/nicotinate mononucleotide adenylyltransferase in Giardia lamblia (GlNMNAT)

doi: 10.1016/j.biopen.2015.11.001

Figure Lengend Snippet: Multiple sequence alignment of 16 homologous NMNAT proteins from phylogenetically divergent organisms with GlNMNAT isoenzymes . The percentage of conservation is displayed throughout the sequence in bars. Alignment was done with the ClustalO algorithm in the CLC Sequence Viewer v7.0.2 program (CLCBio A/S, Additional Alignments plugin v.1.5.1).

Article Snippet: The multiple alignment was done with ClustalO (CLC Sequence Viewer v7.0.2 program, CLCBio A/S, Additional Alignments plugin v.1.5.1).

Techniques: Sequencing

Journal: Biochimie Open

Article Title: Identification of a nicotinamide/nicotinate mononucleotide adenylyltransferase in Giardia lamblia (GlNMNAT)

doi: 10.1016/j.biopen.2015.11.001

Figure Lengend Snippet: Alignment between the three NMNAT human isoenzymes and the two NMNAT isoenzymes from Giardia lamblia . Conservation percentage per residue position is observed in pink bars. The multiple alignment was done with ClustalO (CLC Sequence Viewer v7.0.2 program, CLCBio A/S, Additional Alignments plugin v.1.5.1). Identity percentages calculated with BLASTP algorithm (NCBI) and shown in the adjacent table. ATP active site motif for recognition and binding in N-terminus and C-terminus regions depicted in red (GxFxPx[H/T]xxH) and violet respectively (ISSTxxR) . (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The multiple alignment was done with ClustalO (CLC Sequence Viewer v7.0.2 program, CLCBio A/S, Additional Alignments plugin v.1.5.1).

Techniques: Residue, Sequencing, Binding Assay