sequant zic-hilic column Search Results


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  • 89
    Merck KGaA sequant zic hilic column
    ESI-MS spectra of selected compounds in the Genista tinctoria extract. Column: <t>ZIC-HILIC</t> (100 × 2.1 mm, 3.5 µ m). Elution gradient: 0–4 min 98% ACN, 6-7 min 90%, 8–8.4 min 80%, 8.4–12 min 50%, and 13–20 min 98%.
    Sequant Zic Hilic Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 89/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic hilic column/product/Merck KGaA
    Average 89 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    sequant zic hilic column - by Bioz Stars, 2020-08
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    90
    Merck & Co sequant zic hilic column
    Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using <t>ZIC-HILIC-MS.</t> In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.
    Sequant Zic Hilic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic hilic column/product/Merck & Co
    Average 90 stars, based on 120 article reviews
    Price from $9.99 to $1999.99
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    92
    Hichrom Limited sequant zic hilic column
    Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using <t>ZIC-HILIC-MS.</t> In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.
    Sequant Zic Hilic Column, supplied by Hichrom Limited, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic hilic column/product/Hichrom Limited
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
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    91
    Millipore sequant zic hilic column
    Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using <t>ZIC-HILIC-MS.</t> In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.
    Sequant Zic Hilic Column, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic hilic column/product/Millipore
    Average 91 stars, based on 39 article reviews
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    sequant zic hilic column - by Bioz Stars, 2020-08
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    91
    The Nest Group sequant zic hilic column
    Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using <t>ZIC-HILIC-MS.</t> In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.
    Sequant Zic Hilic Column, supplied by The Nest Group, used in various techniques. Bioz Stars score: 91/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic hilic column/product/The Nest Group
    Average 91 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    sequant zic hilic column - by Bioz Stars, 2020-08
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    90
    Merck KGaA sequant zic hilic hplc guard column
    Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using <t>ZIC-HILIC-MS.</t> In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.
    Sequant Zic Hilic Hplc Guard Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic hilic hplc guard column/product/Merck KGaA
    Average 90 stars, based on 2 article reviews
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    93
    Merck & Co sequant zic hilic hydrophilic columns
    Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using <t>ZIC-HILIC-MS.</t> In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.
    Sequant Zic Hilic Hydrophilic Columns, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic hilic hydrophilic columns/product/Merck & Co
    Average 93 stars, based on 5 article reviews
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    88
    Merck & Co sequant zic hilic guard column
    Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using <t>ZIC-HILIC-MS.</t> In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.
    Sequant Zic Hilic Guard Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic hilic guard column/product/Merck & Co
    Average 88 stars, based on 15 article reviews
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    90
    Merck & Co sequant zic hilic hplc column
    Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using <t>ZIC-HILIC-MS.</t> In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.
    Sequant Zic Hilic Hplc Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic hilic hplc column/product/Merck & Co
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    92
    Millipore sequant zic hydrophilic interaction liquid chromatographic hilic column
    Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using <t>ZIC-HILIC-MS.</t> In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.
    Sequant Zic Hydrophilic Interaction Liquid Chromatographic Hilic Column, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic hydrophilic interaction liquid chromatographic hilic column/product/Millipore
    Average 92 stars, based on 18 article reviews
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    93
    Millipore sequant zic hilic peek column
    Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using <t>ZIC-HILIC-MS.</t> In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.
    Sequant Zic Hilic Peek Column, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck KGaA reversed phase column sequant zic hilic
    Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using <t>ZIC-HILIC-MS.</t> In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.
    Reversed Phase Column Sequant Zic Hilic, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reversed phase column sequant zic hilic/product/Merck KGaA
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    84
    Merck & Co sequantr zic hilic column
    Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using <t>ZIC-HILIC-MS.</t> In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.
    Sequantr Zic Hilic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequantr zic hilic column/product/Merck & Co
    Average 84 stars, based on 1 article reviews
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    84
    Merck KGaA 100a sequantz zic hilic column
    Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using <t>ZIC-HILIC-MS.</t> In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.
    100a Sequantz Zic Hilic Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/100a sequantz zic hilic column/product/Merck KGaA
    Average 84 stars, based on 1 article reviews
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    91
    Merck & Co sequant zic chilic column
    Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using <t>ZIC-HILIC-MS.</t> In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.
    Sequant Zic Chilic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic chilic column/product/Merck & Co
    Average 91 stars, based on 18 article reviews
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    Image Search Results


    ESI-MS spectra of selected compounds in the Genista tinctoria extract. Column: ZIC-HILIC (100 × 2.1 mm, 3.5 µ m). Elution gradient: 0–4 min 98% ACN, 6-7 min 90%, 8–8.4 min 80%, 8.4–12 min 50%, and 13–20 min 98%.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Application of Hydrophilic Interaction Liquid Chromatography for the Quantification of Flavonoids in Genista tinctoria Extract

    doi: 10.1155/2016/3789348

    Figure Lengend Snippet: ESI-MS spectra of selected compounds in the Genista tinctoria extract. Column: ZIC-HILIC (100 × 2.1 mm, 3.5 µ m). Elution gradient: 0–4 min 98% ACN, 6-7 min 90%, 8–8.4 min 80%, 8.4–12 min 50%, and 13–20 min 98%.

    Article Snippet: Compounds were separated on two chromatographic columns: Atlantis-HILIC column (100 × 2.1 mm, 3.0 μ m) from Waters (Milford, MA, USA), and SeQuant™ ZIC-HILIC column (100 × 2.1 mm, 3.5 µ m) from Merck (Darmstadt, Germany).

    Techniques: Mass Spectrometry, Hydrophilic Interaction Liquid Chromatography

    Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using ZIC-HILIC-MS. In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.

    Journal: The Journal of Biological Chemistry

    Article Title: Molecular Identification of d-Ribulokinase in Budding Yeast and Mammals *

    doi: 10.1074/jbc.M116.760744

    Figure Lengend Snippet: Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using ZIC-HILIC-MS. In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.

    Article Snippet: Chromatographic separation was performed using either a SeQuant ZIC-HILIC column (150 × 2.1 mm, 3.5 μm, 100 Å; Merck) fitted with a SeQuant ZIC-HILIC guard column (14 × 1 mm, 5 μm, 200 Å; Merck) or an Acquity UPLC HSS T3 column (150 × 2.1 mm, 1.8 μm, 100 Å; Waters) fitted with a VanGuard Acquity UPLC HSS T3 guard column (5 × 2.1 mm, 1.8 μm, 100 Å; Waters).

    Techniques: Hydrophilic Interaction Liquid Chromatography, Mass Spectrometry

    Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using ZIC-HILIC-MS. In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.

    Journal: The Journal of Biological Chemistry

    Article Title: Molecular Identification of d-Ribulokinase in Budding Yeast and Mammals *

    doi: 10.1074/jbc.M116.760744

    Figure Lengend Snippet: Multivariate statistics analyses of the metabolite profiles obtained for the wild-type and ydr109c Δ prototrophic stains using ZIC-HILIC-MS. In the score plots shown, the green and red oval shapes represent the 95% confidence intervals for the wild-type (WT; green + symbols) and ydr109c Δ (KO; red Δ symbols) replicates, respectively. A, PCA score plot showing principal component 1 ( PC1 ) versus PC2 for the negative mode mTIC normalized data. B, PCA score plot for the positive mode mTIC normalized data. C, PLS-DA score plot showing component 1 versus component 2 for the negative mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.99 and Q 2 = 0.98 for component 1, and R 2 = 1.00 and Q 2 = 0.99 for component 2. D, PLS-DA score plot for the positive mode mTIC normalized data. Leave-one-out cross-validation statistics were R 2 = 0.98 and Q 2 = 0.96 for component 1 and R 2 = 0.99 and Q 2 = 0.96 for component 2. mTIC, metabolic total ion chromatogram.

    Article Snippet: Chromatographic separation was performed using either a SeQuant ZIC-HILIC column (150 × 2.1 mm, 3.5 μm, 100 Å; Merck) fitted with a SeQuant ZIC-HILIC guard column (14 × 1 mm, 5 μm, 200 Å; Merck) or an Acquity UPLC HSS T3 column (150 × 2.1 mm, 1.8 μm, 100 Å; Waters) fitted with a VanGuard Acquity UPLC HSS T3 guard column (5 × 2.1 mm, 1.8 μm, 100 Å; Waters).

    Techniques: Hydrophilic Interaction Liquid Chromatography, Mass Spectrometry