Journal: PLoS ONE

Article Title: GPR54-Dependent Stimulation of Luteinizing Hormone Secretion by Neurokinin B in Prepubertal Rats

doi: 10.1371/journal.pone.0044344

Figure Lengend Snippet: Representative LH profiles demonstrating the effect of icv administration (long arrow) of a selective NK3R agonist, senktide ( C, D, E ), or vehicle ( A , B ) on pulsatile LH secretion in gonadal-intact prepubertal female rats in the presence ( A , B , D, E ; short arrows) or absence ( C ) of the selective GPR54 antagonist, Kp-234. Central administration of senktide induced a single LH pulse ( C ), while intermittent infusion of Kp-234 had no effect on basal LH levels ( A , B ). Kp-234 potently blocked senktide-induced LH pulses ( D ). Where spontaneous LH pulses ( B, E ) were detected during the pre-treatment period, these were excluded from analysis by comparing the previous 30-min period devoid of spontaneous LH pulses with the 30-min post-treatment period ( B ). Administration of Kp-234 immediately following a spontaneous LH pulse tended to attenuate the pulse ( B ), as compared to spontaneous LH pulses occurring considerably prior to Kp-234 administration, which were unaffected ( E ). The 30-min post-treatment period commenced at the time of the senktide/vehicle injection (long arrow). Area under the curve (AUC) values in the 30-min pre-treatment (baseline) period (AUC1) and the 30-min post-treatment period (AUC2) for the three treatment groups are compared in the experiment summary ( F) . * P <0.05 versus 30-min pre-treatment (baseline) period within the same treatment group, as well as versus the same 30-min period within the group treated with Kp-234 and senktide; n = 3−9 per group.

Article Snippet: Infusion regimen consisted of three consecutive injections of vehicle or 2 nmol selective GPR54 antagonist, Kp-234 (Tocris, Bristol, UK), each administered over 5 min, 20 min apart, with a further injection of vehicle or 300 pmol selective NK3R agonist, senktide (Tocris), administered 15 min following the first injection.

Techniques: Injection

Journal: PLoS ONE

Article Title: GPR54-Dependent Stimulation of Luteinizing Hormone Secretion by Neurokinin B in Prepubertal Rats

doi: 10.1371/journal.pone.0044344

Figure Lengend Snippet: Representative LH profiles demonstrating the effect of icv administration (long arrow) of a selective NK3R agonist, senktide ( C , D ), or vehicle ( A , B ) on pulsatile LH secretion in gonadal-intact prepubertal female rats in the presence (short arrows) of the selective KOR agonist, U50488 ( A , C ), or the selective KOR antagonist, nor-BNI ( B , D ). Central infusion of U50488 or nor-BNI had no effect on basal LH levels ( A , B ). Likewise, neither U50488, nor nor-BNI, affected the senktide-induced LH pulses ( C , D ). The 30-min post-treatment period commenced at the time of the senktide/vehicle injection (long arrow). Area under the curve (AUC) values in the 30-min pre-treatment (baseline) period ( AUC1 ) and the 30-min post-treatment period ( AUC2 ) for the five treatment groups are compared in the experiment summary ( E) . * P <0.05 versus 30-min pre-treatment (baseline) period within the same treatment group, as well as versus the same 30-min period within the group treated with U50488 and vehicle, and with nor-BNI and vehicle; n = 4−10 per group.

Article Snippet: Infusion regimen consisted of three consecutive injections of vehicle or 2 nmol selective GPR54 antagonist, Kp-234 (Tocris, Bristol, UK), each administered over 5 min, 20 min apart, with a further injection of vehicle or 300 pmol selective NK3R agonist, senktide (Tocris), administered 15 min following the first injection.

Techniques: Injection

Journal: Endocrine Journal

Article Title: Neurokinin B receptor agonist and Dynorphin receptor antagonist stimulated luteinizing hormone secretion in fasted male rodents

doi: 10.1507/endocrj.ej17-0136

Figure Lengend Snippet: Fig. 2 Hypothalamic Kiss1, Kiss1r, GnRH, Tac3, Tacr3, Pdyn and Oprk1 mRNA expression levels in male rats in experiment 1. Kiss1r and Oprk1 were increased in Fast + senktide group. Pdyn was decreased in Fast + senktide group. Values were normalized by GAPDH as an internal control. Data were expressed as mean + SE values. ** p < 0.01 corresponding groups.

Article Snippet: Fast and Fast + senktide groups were fasted for 72 h. Fed and fast groups were injected with 0.1 mL saline and Fast + senktide group was injected with senktide (Santa Cruz Biotechnology, Inc. California, USA) 20 μg/kg, a NK3R agonist dissolved with saline into intraperitoneally (ip) after 72 h fasting, respectively.

Techniques: Expressing, Control