selective ep2 antagonist Search Results


selective prostaglandin e2 receptor 2 ep2 antagonist  (Cayman Chemical)
99
Cayman Chemical selective prostaglandin e2 receptor 2 ep2 antagonist
Fig. 8. Effects of 27-OHC on prostaglandin (PG) synthesis and effects of cyclooxygenase (COX) inhibitors on 27-OHC-enhanced cellular senes- cence. HFL-1 cells were exposed to various concentrations of 27-OHC for 2 wk. COX-1 and COX-2 expression was evaluated by Western blotting (A and B). <t>PGE2</t> and PGD2 release was quantified by enzyme-linked immunoassay (ELISA) (C and D). Cells were treated with 27-OHC in the presence or absence of indo- methacin, a nonselective COX inhibitor; cele- coxib, a selective COX-2 inhibitor; and PF- 04418948, a selective prostaglandin E2 receptor 2 <t>(EP2)</t> antagonist. Cells were harvested and assayed for 3-NT formation (E) and SA--gal activity (F and G). Relative intensity was calcu- lated by dividing each protein band intensity by the -actin band intensity. Values are means SE (n 4). *P 0.05 and **P 0.01 vs. control group. †P 0.05 and ††P 0.01 vs. 27-OHC-treated group.
Selective Prostaglandin E2 Receptor 2 Ep2 Antagonist, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/selective+ep2+antagonist/Prostaglandin+E2/pm27036870-34-155-168
Average 99 stars, based on 1 article reviews
selective prostaglandin e2 receptor 2 ep2 antagonist - by Bioz Stars, 2026-10
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pf 04418948  (Tocris)
94
Tocris pf 04418948
Fig. 8. Effects of 27-OHC on prostaglandin (PG) synthesis and effects of cyclooxygenase (COX) inhibitors on 27-OHC-enhanced cellular senes- cence. HFL-1 cells were exposed to various concentrations of 27-OHC for 2 wk. COX-1 and COX-2 expression was evaluated by Western blotting (A and B). <t>PGE2</t> and PGD2 release was quantified by enzyme-linked immunoassay (ELISA) (C and D). Cells were treated with 27-OHC in the presence or absence of indo- methacin, a nonselective COX inhibitor; cele- coxib, a selective COX-2 inhibitor; and PF- 04418948, a selective prostaglandin E2 receptor 2 <t>(EP2)</t> antagonist. Cells were harvested and assayed for 3-NT formation (E) and SA--gal activity (F and G). Relative intensity was calcu- lated by dividing each protein band intensity by the -actin band intensity. Values are means SE (n 4). *P 0.05 and **P 0.01 vs. control group. †P 0.05 and ††P 0.01 vs. 27-OHC-treated group.
Pf 04418948, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/selective+ep2+antagonist/PF+04418948/pmc06121445-223-15-23
Average 94 stars, based on 1 article reviews
pf 04418948 - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier

Image Search Results


Fig. 8. Effects of 27-OHC on prostaglandin (PG) synthesis and effects of cyclooxygenase (COX) inhibitors on 27-OHC-enhanced cellular senes- cence. HFL-1 cells were exposed to various concentrations of 27-OHC for 2 wk. COX-1 and COX-2 expression was evaluated by Western blotting (A and B). PGE2 and PGD2 release was quantified by enzyme-linked immunoassay (ELISA) (C and D). Cells were treated with 27-OHC in the presence or absence of indo- methacin, a nonselective COX inhibitor; cele- coxib, a selective COX-2 inhibitor; and PF- 04418948, a selective prostaglandin E2 receptor 2 (EP2) antagonist. Cells were harvested and assayed for 3-NT formation (E) and SA--gal activity (F and G). Relative intensity was calcu- lated by dividing each protein band intensity by the -actin band intensity. Values are means SE (n 4). *P 0.05 and **P 0.01 vs. control group. †P 0.05 and ††P 0.01 vs. 27-OHC-treated group.

Journal: American journal of physiology. Lung cellular and molecular physiology

Article Title: 27-Hydroxycholesterol accelerates cellular senescence in human lung resident cells.

doi: 10.1152/ajplung.00351.2015

Figure Lengend Snippet: Fig. 8. Effects of 27-OHC on prostaglandin (PG) synthesis and effects of cyclooxygenase (COX) inhibitors on 27-OHC-enhanced cellular senes- cence. HFL-1 cells were exposed to various concentrations of 27-OHC for 2 wk. COX-1 and COX-2 expression was evaluated by Western blotting (A and B). PGE2 and PGD2 release was quantified by enzyme-linked immunoassay (ELISA) (C and D). Cells were treated with 27-OHC in the presence or absence of indo- methacin, a nonselective COX inhibitor; cele- coxib, a selective COX-2 inhibitor; and PF- 04418948, a selective prostaglandin E2 receptor 2 (EP2) antagonist. Cells were harvested and assayed for 3-NT formation (E) and SA--gal activity (F and G). Relative intensity was calcu- lated by dividing each protein band intensity by the -actin band intensity. Values are means SE (n 4). *P 0.05 and **P 0.01 vs. control group. †P 0.05 and ††P 0.01 vs. 27-OHC-treated group.

Article Snippet: Commercially available reagents were obtained as follows: mouse monoclonal anti-p53 antibody, mouse monoclonal anti-p16 antibody, mouse monoclonal anti-cyclooygenase-1 (COX-1) antibody, mouse monoclonal anti-COX-2 antibody, mouse monoclonal anti- -actin antibody, and mouse monoclonal anti-lamin A/C antibody were from Santa Cruz Biotechnology (Dallas, TX); rabbit polyclonal anti-p21 antibody, rabbit polyclonal anti-phosphorylated (p)-retinoblastoma (pRB) antibody and rabbit polyclonal anti-p-p53 antibody were from Cell Signaling Technology (Beverly, MA); rabbit polyclonal antisterol 27-hydroxylase antibody and 27-OHC were from Avanti Polar Lipids (Alabaster, AL); mouse anti-CD68 antibody was from BioLegend (San Diego, CA); fibronectin enzyme-linked immunosorbent assay (ELISA) kit, goat fluorescein isothiocyanate polyclonal secondary antibody, and rabbit Dylight 650 polyclonal secondary antibody were from Abcam (Cambridge, UK); indomethacin, a nonselective COX inhibitor, and celecoxib, a selective 27-OHC-2 inhibitor, were from Sigma (St. Louis, MO); manganese (III) tetrakis(4-benzoic acid)porphyrin chloride (MnTBAP), a peroxynitrite scavenger, was from Calbiochem (La Jolla, CA); diaminofluorescein-2, a fluorescent indicator, was from Sekisui Medical (Tokyo, Japan); PF-04418948, a selective prostaglandin E2 receptor 2 (EP2) antagonist and EIA prostaglandin kit were from Cayman Chemical (San Diego, CA); Dulbecco’s Modified Eagle’s Medium (DMEM), fetal calf serum (FCS), and antibiotic-antimycotic were purchased from Invitrogen Life Technologies (Grand Island, NY).

Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Activity Assay, Control