Journal: Laboratory Animal Research

Article Title: Highlights on selected microscopy techniques to study zebrafish developmental biology

doi: 10.1186/s42826-020-00044-2

Figure Lengend Snippet: a A presentative diagram of imaging zebrafish embryo by Second Harmonic Generation Microscopy (SHG) and Third Harmonic Generation (THG): A titanium-sapphire laser generates pulses of 200 fs at 1200 nm. The laser beam passes on galvanometric mirrors (GM) to reach the sample through the objective lens. A dichroic mirror reflects the scattered signals from the sample, and the other dichroic mirror separates them into two channels (THG) and (SHG/2PF). The signals focused on the photomultiplier tube detectors (PMT), one for THG, and the other for SHG and 2PF. b A scheme of the light spectrum in SHG and THG microscopy. The excitation wavelength is 1200 nm, while the emission spectra of THG at 400 nm, SHG at 600 nm, and Two-Photon 2PF from 600 to 800 nm with a peak at ~ 650 nm generated by the sample

Article Snippet: Here, we briefly discuss the engagement of zebrafish in three models of advanced microscopy techniques: Two-Photon Microscopy (TPM), Second Harmonic Generation, (SHG), and Light-Sheet Microscopy (LSM).

Techniques: Imaging, Microscopy, Generated

Journal: Frontiers in Plant Science

Article Title: An update: improvements in imaging perfluorocarbon-mounted plant leaves with implications for studies of plant pathology, physiology, development and cell biology

doi: 10.3389/fpls.2014.00140

Figure Lengend Snippet: Perfluorocarbon mounting media used with three modes of microscopy. (A–C) LSCM images captured from 1 μm resolution Z-stacks taken of samples mounted in H 2 O, PFD and PP11 respectively. Images were generated by using the “cut” function in Zeiss LSM Image Browser software, where the plane presented represents a cut through the entire z-stack from top to bottom taken at an angle of -24°. The images therefore show a progression through the stack from top to bottom. Signal was collected from 518 to 604 nm for Venus, and is displayed in green and at 647–690 nm for chlorophyll, shown in red. (D–F) 2-photon micrographs taken of samples mounted in H 2 O, PFD, and PP11 respectively. (G–I) SHG micrographs taken of samples mounted in H 2 O, PFD, and PP11 respectively. (D–I) are presented as projections of z-stacks. Scale bars are 50 μm.

Article Snippet: A diverse palette of chemical labels and genetically encoded fluorescent reporters and biosensors, coupled with advanced microscopy techniques, including laser scanning confocal microscopy (LSCM), two-photon fluorescence (TPF) microscopy and label-free imaging techniques such as second harmonic generation (SHG) microscopy and stimulated Raman scattering (SRS) microscopy have enabled unprecedented analysis of living cell dynamics ( ).

Techniques: Microscopy, Generated, Software