Journal: Protein Science : A Publication of the Protein Society

Article Title: Elastin‐like polypeptide switches: A design strategy to detect multimeric proteins

doi: 10.1002/pro.3215

Figure Lengend Snippet: Identification of a high molecular weight ELP that detects streptavidin‐biotin interactions. (A) SDS‐PAGE confirmed the identity and purity of an ELP, 2VA192 (Table I). (B) Optical density was used to characterize the concentration‐temperature phase diagrams for a library of ELPs with increasing chain lengths. ELPs phase separate above the indicated best‐fit lines. (C) Similarly, biotin‐2VA192 (biotin‐ELP) with and without a stoichiometric ratio of streptavidin [4:1] was characterized. The best‐fit line and its 95% confidence interval are depicted.

Article Snippet: 35 Purity and identity was determined by loading 5 µg ELP in SDS loading buffer, heating at 95°C for 5 min, and running on a 4–20% gradient Tris‐Glycine SDS PAGE gel (58505, Lonza, Walkersville, MD).

Techniques: Molecular Weight, SDS Page, Concentration Assay