Journal: Proceedings of the National Academy of Sciences of the United States of America
Article Title: Human-like hyperplastic prostate with low ZIP1 induced solely by Zn deficiency in rats
Figure Lengend Snippet: Establishment of a Zn-deficient middle-aged Sprague–Dawley rat model with prostatic Zn loss. ( A ) Study design: 1-mo-old male rats received a Zn-deficient or Zn-sufficient diet for 1.5, 4, or 10 mo to form six Zn-modulated age groups ( n = 10–20 rats per group): Zn-deficient young-adult, adult, and middle-aged and Zn-sufficient young-adult, adult, and middle-aged. ( B and C ) Testis Zn content ( B ) and prostate Zn content ( C ) (measured in micrograms per gram dry weight) of young-adult, adult, and middle-aged rats on a Zn-deficient or a Zn-sufficient diet ( n = 7–12 rats per group). ( D ) The PCNA-labeling index in middle-aged prostate is expressed as the percent of intensely stained PCNA-positive nuclei (S-phase) per ∼500 prostate epithelial nuclei evaluated in a microscope field at 200× magnification ( n = 9 rats per group). ( E ) NF-κβ p65 DNA-binding activity of nuclear extracts from middle-aged rat prostates was measured by the TransAM NF-κβ p65 assay kit ( n = 9 rats per group). ( F ) qPCR analysis of four selected inflammation genes, S100a8 , S100a9 , Cxcl5 , and Ptgs 2, in middle-aged rat prostates ( n = 6–10 rats per group, measurements were performed in triplicate with Psmb6 as a normalizer). Data are expressed as mean ± SD. All statistics are two-sided; * P
Article Snippet: We used two rat strains, the Sprague–Dawley (Taconic) and Wistar-Unilever (HsdCpb:Wu) (Envigo) strains, and custom-formulated Zn-deficient (3–4 ppm Zn) and Zn-sufficient (∼60 ppm Zn) diets from Harlan Teklad.
Techniques: Labeling, Staining, Microscopy, Binding Assay, Activity Assay, Real-time Polymerase Chain Reaction