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  • 92
    Thermo Fisher sds out pierce sds precipitation kit
    Sds Out Pierce Sds Precipitation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 6 article reviews
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    94
    Millipore sd 208 sd 208
    Colon adenocarcinoma (grade IV) stained immunohistochemically with anti-Ki-67. Positive immunoreactive showing brown nuclear expression of Ki-67 with a large number (~80%) of stained nuclei reflecting active cellular proliferation in the treated nude mice with <t>SD-208</t> (A) and control (B) : Immunohischemical assay showed no significant difference between tests and controls in terms of cellular proliferation (P > 0.05).
    Sd 208 Sd 208, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sd 208 sd 208/product/Millipore
    Average 94 stars, based on 2 article reviews
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    99
    Bio-Rad sds soluble
    Analysis of cellular protein co-sedimentation with tau aggregates in mouse N2a cell models. (a) Average DOC-insoluble spectral counts in the seeded N2a cells for a subset of proteins that were identified as aberrantly fractionating to <t>SDS-insoluble</t> fractions from the brains of rTg4510 mice. (b) Average <t>PBS-soluble</t> spectral counts for the same proteins displayed in panel (a), indicating the level of detectability of the proteins in PBS-soluble fractions. (c) Spectral count data for the small number of proteins that meet criteria for over-representation in DOC-insoluble fractions from N2a cells seeded for tau aggregation. Proteins were accepted if they (i) achieved at least a 3-fold increase in DOC-insoluble spectra from untransfected to tau seeded cells for at least 2 out of 3 experimental replicates and (ii) during instances where untransfected cells yielded 0 peptides for any given proteins, the tau seeded condition yielded a significant G-test (p
    Sds Soluble, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sds soluble/product/Bio-Rad
    Average 99 stars, based on 13 article reviews
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    99
    Taconic Biosciences sd ntac
    Level of activity, the total number of lever presses, by <t>SHR/NCrl,</t> WKY/NHsd, <t>SD/NTac</t> Sprague Dawley, WH/HanTac Wistar, and WKY/NCrl strains. The final schedule was introduced at session 13 (Means ± 1 SEM).
    Sd Ntac, supplied by Taconic Biosciences, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sd ntac/product/Taconic Biosciences
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    94
    Selleck Chemicals sd 208
    Level of activity, the total number of lever presses, by <t>SHR/NCrl,</t> WKY/NHsd, <t>SD/NTac</t> Sprague Dawley, WH/HanTac Wistar, and WKY/NCrl strains. The final schedule was introduced at session 13 (Means ± 1 SEM).
    Sd 208, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sd 208/product/Selleck Chemicals
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    sds  (Lonza)
    92
    Lonza sds
    Level of activity, the total number of lever presses, by <t>SHR/NCrl,</t> WKY/NHsd, <t>SD/NTac</t> Sprague Dawley, WH/HanTac Wistar, and WKY/NCrl strains. The final schedule was introduced at session 13 (Means ± 1 SEM).
    Sds, supplied by Lonza, used in various techniques. Bioz Stars score: 92/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sds/product/Lonza
    Average 92 stars, based on 37 article reviews
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    Image Search Results


    Colon adenocarcinoma (grade IV) stained immunohistochemically with anti-Ki-67. Positive immunoreactive showing brown nuclear expression of Ki-67 with a large number (~80%) of stained nuclei reflecting active cellular proliferation in the treated nude mice with SD-208 (A) and control (B) : Immunohischemical assay showed no significant difference between tests and controls in terms of cellular proliferation (P > 0.05).

    Journal: DARU Journal of Pharmaceutical Sciences

    Article Title: Evaluation of antitumor activity of a TGF-beta receptor I inhibitor (SD-208) on human colon adenocarcinoma

    doi: 10.1186/2008-2231-22-47

    Figure Lengend Snippet: Colon adenocarcinoma (grade IV) stained immunohistochemically with anti-Ki-67. Positive immunoreactive showing brown nuclear expression of Ki-67 with a large number (~80%) of stained nuclei reflecting active cellular proliferation in the treated nude mice with SD-208 (A) and control (B) : Immunohischemical assay showed no significant difference between tests and controls in terms of cellular proliferation (P > 0.05).

    Article Snippet: Chemical description and biological activity TGβRI kinase inhibitor, SD-208 SD-208 (Sigma Aldrich; Belgium) is a selective and orally active pyridopyrimidine type TGβRI kinase inhibitor with an IC50 of approximately 35 nmol/L against TβRI kinase activity in vitro .

    Techniques: Staining, Expressing, Mouse Assay

    Effect of SD-208 on the cell growth and proliferation of the SW-48 cells. SW-48 cells were treated by 0.5, 1 and 2 μM for 48 h. Cell proliferation was examined by MTT and BrdU assays as described in methods. A : MTT assay of SW-48 cells after treatment with SD-208 in comparison with controls (untreated and treated with DMSO). B : BrdU assay of SW-48 cells after treatment with SD-208 comparison with controls (untreated and treated with DMSO). All data are reported as the percentage change in comparison with the controls, which were arbitrarily assigned 100% cell proliferation. Analysis of one-way ANOVA was used to compare the cell proliferation of SW-48 cells in different concentrations of SD-208 to control. P value

    Journal: DARU Journal of Pharmaceutical Sciences

    Article Title: Evaluation of antitumor activity of a TGF-beta receptor I inhibitor (SD-208) on human colon adenocarcinoma

    doi: 10.1186/2008-2231-22-47

    Figure Lengend Snippet: Effect of SD-208 on the cell growth and proliferation of the SW-48 cells. SW-48 cells were treated by 0.5, 1 and 2 μM for 48 h. Cell proliferation was examined by MTT and BrdU assays as described in methods. A : MTT assay of SW-48 cells after treatment with SD-208 in comparison with controls (untreated and treated with DMSO). B : BrdU assay of SW-48 cells after treatment with SD-208 comparison with controls (untreated and treated with DMSO). All data are reported as the percentage change in comparison with the controls, which were arbitrarily assigned 100% cell proliferation. Analysis of one-way ANOVA was used to compare the cell proliferation of SW-48 cells in different concentrations of SD-208 to control. P value

    Article Snippet: Chemical description and biological activity TGβRI kinase inhibitor, SD-208 SD-208 (Sigma Aldrich; Belgium) is a selective and orally active pyridopyrimidine type TGβRI kinase inhibitor with an IC50 of approximately 35 nmol/L against TβRI kinase activity in vitro .

    Techniques: MTT Assay, BrdU Staining

    A representative of colorectal adenocarcinoma model. Tumor implantation; athymic nude mice implanted by SW-48 cell line, the cells were grown as tumor xenografts after 10 days. Cancer-bearing nude mice were treated with 50 mg/kg/day of SD-208 (A) or without SD-208 (B) .

    Journal: DARU Journal of Pharmaceutical Sciences

    Article Title: Evaluation of antitumor activity of a TGF-beta receptor I inhibitor (SD-208) on human colon adenocarcinoma

    doi: 10.1186/2008-2231-22-47

    Figure Lengend Snippet: A representative of colorectal adenocarcinoma model. Tumor implantation; athymic nude mice implanted by SW-48 cell line, the cells were grown as tumor xenografts after 10 days. Cancer-bearing nude mice were treated with 50 mg/kg/day of SD-208 (A) or without SD-208 (B) .

    Article Snippet: Chemical description and biological activity TGβRI kinase inhibitor, SD-208 SD-208 (Sigma Aldrich; Belgium) is a selective and orally active pyridopyrimidine type TGβRI kinase inhibitor with an IC50 of approximately 35 nmol/L against TβRI kinase activity in vitro .

    Techniques: Tumor Implantation, Mouse Assay

    A representative results of pathological examinations of nude mice tumors with or without SD-208 treatment. A : tumors of nude mice treated with 50 mg/kg/day stained with H E. B : H E staining of tumor tissues of control. No significant difference histologically was observed (P > 0.05).

    Journal: DARU Journal of Pharmaceutical Sciences

    Article Title: Evaluation of antitumor activity of a TGF-beta receptor I inhibitor (SD-208) on human colon adenocarcinoma

    doi: 10.1186/2008-2231-22-47

    Figure Lengend Snippet: A representative results of pathological examinations of nude mice tumors with or without SD-208 treatment. A : tumors of nude mice treated with 50 mg/kg/day stained with H E. B : H E staining of tumor tissues of control. No significant difference histologically was observed (P > 0.05).

    Article Snippet: Chemical description and biological activity TGβRI kinase inhibitor, SD-208 SD-208 (Sigma Aldrich; Belgium) is a selective and orally active pyridopyrimidine type TGβRI kinase inhibitor with an IC50 of approximately 35 nmol/L against TβRI kinase activity in vitro .

    Techniques: Mouse Assay, Staining

    Colon adenocarcinoma (grade IV) stained immunohistochemically with anti-CD34. The paraffin embedded sections of tumor tissues were stained with anti-CD34 antibody and positive immunoreactive indicating brown cytoplasmic membrane of endothelial cells and marked microvessel's proliferation. Microvessel density (MVD) was ~40 microvessel/mm 2 . SD-208-treated tumors (A) vs controls (B) revealed no significant immunohistologically differences (P > 0.05). Note the prominent vascularity. Arrows indicate CD34 staining of the cytoplasmic membrane.

    Journal: DARU Journal of Pharmaceutical Sciences

    Article Title: Evaluation of antitumor activity of a TGF-beta receptor I inhibitor (SD-208) on human colon adenocarcinoma

    doi: 10.1186/2008-2231-22-47

    Figure Lengend Snippet: Colon adenocarcinoma (grade IV) stained immunohistochemically with anti-CD34. The paraffin embedded sections of tumor tissues were stained with anti-CD34 antibody and positive immunoreactive indicating brown cytoplasmic membrane of endothelial cells and marked microvessel's proliferation. Microvessel density (MVD) was ~40 microvessel/mm 2 . SD-208-treated tumors (A) vs controls (B) revealed no significant immunohistologically differences (P > 0.05). Note the prominent vascularity. Arrows indicate CD34 staining of the cytoplasmic membrane.

    Article Snippet: Chemical description and biological activity TGβRI kinase inhibitor, SD-208 SD-208 (Sigma Aldrich; Belgium) is a selective and orally active pyridopyrimidine type TGβRI kinase inhibitor with an IC50 of approximately 35 nmol/L against TβRI kinase activity in vitro .

    Techniques: Staining

    Continuous culture of SW-48 cells and treatment with SD-208. The cells were grown as monolayer epithelial-like morphology. SW-48 cells after treatment by DMSO alone as control (A) , SD-208 concentrations 0.5 μM (B) , 1 μM (C) and 2 μM (D) .

    Journal: DARU Journal of Pharmaceutical Sciences

    Article Title: Evaluation of antitumor activity of a TGF-beta receptor I inhibitor (SD-208) on human colon adenocarcinoma

    doi: 10.1186/2008-2231-22-47

    Figure Lengend Snippet: Continuous culture of SW-48 cells and treatment with SD-208. The cells were grown as monolayer epithelial-like morphology. SW-48 cells after treatment by DMSO alone as control (A) , SD-208 concentrations 0.5 μM (B) , 1 μM (C) and 2 μM (D) .

    Article Snippet: Chemical description and biological activity TGβRI kinase inhibitor, SD-208 SD-208 (Sigma Aldrich; Belgium) is a selective and orally active pyridopyrimidine type TGβRI kinase inhibitor with an IC50 of approximately 35 nmol/L against TβRI kinase activity in vitro .

    Techniques:

    Analysis of cellular protein co-sedimentation with tau aggregates in mouse N2a cell models. (a) Average DOC-insoluble spectral counts in the seeded N2a cells for a subset of proteins that were identified as aberrantly fractionating to SDS-insoluble fractions from the brains of rTg4510 mice. (b) Average PBS-soluble spectral counts for the same proteins displayed in panel (a), indicating the level of detectability of the proteins in PBS-soluble fractions. (c) Spectral count data for the small number of proteins that meet criteria for over-representation in DOC-insoluble fractions from N2a cells seeded for tau aggregation. Proteins were accepted if they (i) achieved at least a 3-fold increase in DOC-insoluble spectra from untransfected to tau seeded cells for at least 2 out of 3 experimental replicates and (ii) during instances where untransfected cells yielded 0 peptides for any given proteins, the tau seeded condition yielded a significant G-test (p

    Journal: Acta neuropathologica

    Article Title: Changes in proteome solubility indicate widespread proteostatic disruption in mouse models of neurodegenerative disease

    doi: 10.1007/s00401-018-1895-y

    Figure Lengend Snippet: Analysis of cellular protein co-sedimentation with tau aggregates in mouse N2a cell models. (a) Average DOC-insoluble spectral counts in the seeded N2a cells for a subset of proteins that were identified as aberrantly fractionating to SDS-insoluble fractions from the brains of rTg4510 mice. (b) Average PBS-soluble spectral counts for the same proteins displayed in panel (a), indicating the level of detectability of the proteins in PBS-soluble fractions. (c) Spectral count data for the small number of proteins that meet criteria for over-representation in DOC-insoluble fractions from N2a cells seeded for tau aggregation. Proteins were accepted if they (i) achieved at least a 3-fold increase in DOC-insoluble spectra from untransfected to tau seeded cells for at least 2 out of 3 experimental replicates and (ii) during instances where untransfected cells yielded 0 peptides for any given proteins, the tau seeded condition yielded a significant G-test (p

    Article Snippet: 30 μL of SDS-insoluble or 5 μL of PBS-soluble protein fractions from each animal were loaded onto a Criterion 4–20% tris-glycine gel (Bio-Rad, Hercules, CA).

    Techniques: Sedimentation, Mouse Assay

    Protein biosynthesis assessed by protein fractionation and Western blot analysis. ( A ) Silver stained SDS containing polyacrylamide gel-resolved protein fractions were assessed for the protein abundance variations in RCs incubated in the axenic media with different carbon sources as in Fig. 5 . ( B ) As in panel A, except that the resolved proteins were transferred to a nylon membrane and assessed by Western blot analysis using mouse polyclonal serum against E . chaffeensis .

    Journal: Scientific Reports

    Article Title: Protein and DNA synthesis demonstrated in cell-free Ehrlichia chaffeensis organisms in axenic medium

    doi: 10.1038/s41598-018-27574-z

    Figure Lengend Snippet: Protein biosynthesis assessed by protein fractionation and Western blot analysis. ( A ) Silver stained SDS containing polyacrylamide gel-resolved protein fractions were assessed for the protein abundance variations in RCs incubated in the axenic media with different carbon sources as in Fig. 5 . ( B ) As in panel A, except that the resolved proteins were transferred to a nylon membrane and assessed by Western blot analysis using mouse polyclonal serum against E . chaffeensis .

    Article Snippet: For normalization of bacterial total protein content, the suspensions of E . chaffeensis cell-free fractions were lysed in 1% SDS solution for 5 min at 100 °C and the total protein concentration was determined using Protein Assay kit (Bio-Rad, Hercules, CA).

    Techniques: Fractionation, Western Blot, Staining, Incubation

    Level of activity, the total number of lever presses, by SHR/NCrl, WKY/NHsd, SD/NTac Sprague Dawley, WH/HanTac Wistar, and WKY/NCrl strains. The final schedule was introduced at session 13 (Means ± 1 SEM).

    Journal: Behavioral and Brain Functions : BBF

    Article Title: Behavioral and genetic evidence for a novel animal model of Attention-Deficit/Hyperactivity Disorder Predominantly Inattentive Subtype

    doi: 10.1186/1744-9081-4-56

    Figure Lengend Snippet: Level of activity, the total number of lever presses, by SHR/NCrl, WKY/NHsd, SD/NTac Sprague Dawley, WH/HanTac Wistar, and WKY/NCrl strains. The final schedule was introduced at session 13 (Means ± 1 SEM).

    Article Snippet: These included 8 Spontaneously Hypertensive (SHR/NCrl) and 12 Wistar/Kyoto (WKY/NCrl) rats from Charles River (Sulzfeld, Germany); 8 SD/NTac (aka NTac:SD) Sprague Dawley and 8 WH/HanTac (aka HanTac:WH) Wistar Hannover GALAS rats from Taconic Europe, Ry, Denmark; as well as 8 WKY/NHsd Wistar/Kyoto rats from Harlan Europe (Blacktorn, Bicester, UK).

    Techniques: Activity Assay

    Development of sustained attention, choice of the correct lever in percent of all lever presses, by SHR/NCrl, WKY/NHsd, SD/NTac Sprague Dawley, WH/HanTac Wistar, and WKY/NCrl strains. The final schedule was introduced at session 13 (Means ± 1 SEM).

    Journal: Behavioral and Brain Functions : BBF

    Article Title: Behavioral and genetic evidence for a novel animal model of Attention-Deficit/Hyperactivity Disorder Predominantly Inattentive Subtype

    doi: 10.1186/1744-9081-4-56

    Figure Lengend Snippet: Development of sustained attention, choice of the correct lever in percent of all lever presses, by SHR/NCrl, WKY/NHsd, SD/NTac Sprague Dawley, WH/HanTac Wistar, and WKY/NCrl strains. The final schedule was introduced at session 13 (Means ± 1 SEM).

    Article Snippet: These included 8 Spontaneously Hypertensive (SHR/NCrl) and 12 Wistar/Kyoto (WKY/NCrl) rats from Charles River (Sulzfeld, Germany); 8 SD/NTac (aka NTac:SD) Sprague Dawley and 8 WH/HanTac (aka HanTac:WH) Wistar Hannover GALAS rats from Taconic Europe, Ry, Denmark; as well as 8 WKY/NHsd Wistar/Kyoto rats from Harlan Europe (Blacktorn, Bicester, UK).

    Techniques:

    Level of impulsiveness, responding within 0.67 s following the previous lever press, by SHR/NCrl, WKY/NHsd, SD/NTac Sprague Dawley, WH/HanTac Wistar, and WKY/NCrl strains following log10 transformation. The final schedule was introduced at session 13 (Means ± 1 SEM).

    Journal: Behavioral and Brain Functions : BBF

    Article Title: Behavioral and genetic evidence for a novel animal model of Attention-Deficit/Hyperactivity Disorder Predominantly Inattentive Subtype

    doi: 10.1186/1744-9081-4-56

    Figure Lengend Snippet: Level of impulsiveness, responding within 0.67 s following the previous lever press, by SHR/NCrl, WKY/NHsd, SD/NTac Sprague Dawley, WH/HanTac Wistar, and WKY/NCrl strains following log10 transformation. The final schedule was introduced at session 13 (Means ± 1 SEM).

    Article Snippet: These included 8 Spontaneously Hypertensive (SHR/NCrl) and 12 Wistar/Kyoto (WKY/NCrl) rats from Charles River (Sulzfeld, Germany); 8 SD/NTac (aka NTac:SD) Sprague Dawley and 8 WH/HanTac (aka HanTac:WH) Wistar Hannover GALAS rats from Taconic Europe, Ry, Denmark; as well as 8 WKY/NHsd Wistar/Kyoto rats from Harlan Europe (Blacktorn, Bicester, UK).

    Techniques: Transformation Assay

    Genotypic differences between SD/NTac Sprague Dawley, SHR/NCrl, WKY/NCrl, and WKY/NHsd strains. (A) For three SSLPs the WKY/NCrl products are the same size as SHR/NCrl. (B) Other SSLPs are the same size for WKY/NCrl and WKY/NHsd (e.g., D3MGH16), indicating that the at least some of the WKY/NHsd background may still be present in the WKY/NCrl.

    Journal: Behavioral and Brain Functions : BBF

    Article Title: Behavioral and genetic evidence for a novel animal model of Attention-Deficit/Hyperactivity Disorder Predominantly Inattentive Subtype

    doi: 10.1186/1744-9081-4-56

    Figure Lengend Snippet: Genotypic differences between SD/NTac Sprague Dawley, SHR/NCrl, WKY/NCrl, and WKY/NHsd strains. (A) For three SSLPs the WKY/NCrl products are the same size as SHR/NCrl. (B) Other SSLPs are the same size for WKY/NCrl and WKY/NHsd (e.g., D3MGH16), indicating that the at least some of the WKY/NHsd background may still be present in the WKY/NCrl.

    Article Snippet: These included 8 Spontaneously Hypertensive (SHR/NCrl) and 12 Wistar/Kyoto (WKY/NCrl) rats from Charles River (Sulzfeld, Germany); 8 SD/NTac (aka NTac:SD) Sprague Dawley and 8 WH/HanTac (aka HanTac:WH) Wistar Hannover GALAS rats from Taconic Europe, Ry, Denmark; as well as 8 WKY/NHsd Wistar/Kyoto rats from Harlan Europe (Blacktorn, Bicester, UK).

    Techniques:

    Effect of Lifor on cold storage injury in kidney slices Kidney slices (n=3 at each timepoint) from Sprague-Dawley rats were cold stored at 4°C for 2 hours, 4 hours, 8 hours or 24 hours. Tissue injury was measured by LDH release. (a) Storage of kidney slices in either UW or Lifor significantly mitigated LDH release compared to media. Activation of caspase-3 was detected by western blot analysis of cleaved caspase-3. (b) Lifor significantly attenuated activation of caspase-3 in kidney slices after 24 hours of cold storage compared to UW. Mean values ± SE are presented. * P

    Journal: The Journal of surgical research

    Article Title: Protective effect of Lifor solution in experimental renal ischemia-reperfusion injury

    doi: 10.1016/j.jss.2010.08.033

    Figure Lengend Snippet: Effect of Lifor on cold storage injury in kidney slices Kidney slices (n=3 at each timepoint) from Sprague-Dawley rats were cold stored at 4°C for 2 hours, 4 hours, 8 hours or 24 hours. Tissue injury was measured by LDH release. (a) Storage of kidney slices in either UW or Lifor significantly mitigated LDH release compared to media. Activation of caspase-3 was detected by western blot analysis of cleaved caspase-3. (b) Lifor significantly attenuated activation of caspase-3 in kidney slices after 24 hours of cold storage compared to UW. Mean values ± SE are presented. * P

    Article Snippet: This set of experiments was performed on 30 male Sprague-Dawley (SD) rats weighing 220–240 grams that were purchased from Taconic Farms (Germantown, NY).

    Techniques: Activation Assay, Western Blot

    Effect of Lifor on ex vivo warm renal IR injury Kidney slices (n=3 at each timepoint) from Sprague-Dawley rats were incubated with either saline, UW, or Lifor and placed in a hypoxic incubator with 1% O 2, 5% CO 2 at 37°C for 30 minutes, 1 hour, or 2 hours. The tissue slices were then reoxygenated in a 37°C, 5% CO 2 incubator for 2 hours. (a) Incubation of kidney slices with either UW or Lifor significantly mitigated LDH release compared to incubation with saline. (b) Incubation of kidney slices in Lifor significantly attenuated activation of caspase-3 compared to UW following 2 hours hypoxia and 2 hours reoxygenation. Mean values ± SE are presented. * P

    Journal: The Journal of surgical research

    Article Title: Protective effect of Lifor solution in experimental renal ischemia-reperfusion injury

    doi: 10.1016/j.jss.2010.08.033

    Figure Lengend Snippet: Effect of Lifor on ex vivo warm renal IR injury Kidney slices (n=3 at each timepoint) from Sprague-Dawley rats were incubated with either saline, UW, or Lifor and placed in a hypoxic incubator with 1% O 2, 5% CO 2 at 37°C for 30 minutes, 1 hour, or 2 hours. The tissue slices were then reoxygenated in a 37°C, 5% CO 2 incubator for 2 hours. (a) Incubation of kidney slices with either UW or Lifor significantly mitigated LDH release compared to incubation with saline. (b) Incubation of kidney slices in Lifor significantly attenuated activation of caspase-3 compared to UW following 2 hours hypoxia and 2 hours reoxygenation. Mean values ± SE are presented. * P

    Article Snippet: This set of experiments was performed on 30 male Sprague-Dawley (SD) rats weighing 220–240 grams that were purchased from Taconic Farms (Germantown, NY).

    Techniques: Ex Vivo, Incubation, Activation Assay

    Establishment of a Zn-deficient middle-aged Sprague–Dawley rat model with prostatic Zn loss. ( A ) Study design: 1-mo-old male rats received a Zn-deficient or Zn-sufficient diet for 1.5, 4, or 10 mo to form six Zn-modulated age groups ( n = 10–20 rats per group): Zn-deficient young-adult, adult, and middle-aged and Zn-sufficient young-adult, adult, and middle-aged. ( B and C ) Testis Zn content ( B ) and prostate Zn content ( C ) (measured in micrograms per gram dry weight) of young-adult, adult, and middle-aged rats on a Zn-deficient or a Zn-sufficient diet ( n = 7–12 rats per group). ( D ) The PCNA-labeling index in middle-aged prostate is expressed as the percent of intensely stained PCNA-positive nuclei (S-phase) per ∼500 prostate epithelial nuclei evaluated in a microscope field at 200× magnification ( n = 9 rats per group). ( E ) NF-κβ p65 DNA-binding activity of nuclear extracts from middle-aged rat prostates was measured by the TransAM NF-κβ p65 assay kit ( n = 9 rats per group). ( F ) qPCR analysis of four selected inflammation genes, S100a8 , S100a9 , Cxcl5 , and Ptgs 2, in middle-aged rat prostates ( n = 6–10 rats per group, measurements were performed in triplicate with Psmb6 as a normalizer). Data are expressed as mean ± SD. All statistics are two-sided; * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Human-like hyperplastic prostate with low ZIP1 induced solely by Zn deficiency in rats

    doi: 10.1073/pnas.1813956115

    Figure Lengend Snippet: Establishment of a Zn-deficient middle-aged Sprague–Dawley rat model with prostatic Zn loss. ( A ) Study design: 1-mo-old male rats received a Zn-deficient or Zn-sufficient diet for 1.5, 4, or 10 mo to form six Zn-modulated age groups ( n = 10–20 rats per group): Zn-deficient young-adult, adult, and middle-aged and Zn-sufficient young-adult, adult, and middle-aged. ( B and C ) Testis Zn content ( B ) and prostate Zn content ( C ) (measured in micrograms per gram dry weight) of young-adult, adult, and middle-aged rats on a Zn-deficient or a Zn-sufficient diet ( n = 7–12 rats per group). ( D ) The PCNA-labeling index in middle-aged prostate is expressed as the percent of intensely stained PCNA-positive nuclei (S-phase) per ∼500 prostate epithelial nuclei evaluated in a microscope field at 200× magnification ( n = 9 rats per group). ( E ) NF-κβ p65 DNA-binding activity of nuclear extracts from middle-aged rat prostates was measured by the TransAM NF-κβ p65 assay kit ( n = 9 rats per group). ( F ) qPCR analysis of four selected inflammation genes, S100a8 , S100a9 , Cxcl5 , and Ptgs 2, in middle-aged rat prostates ( n = 6–10 rats per group, measurements were performed in triplicate with Psmb6 as a normalizer). Data are expressed as mean ± SD. All statistics are two-sided; * P

    Article Snippet: We used two rat strains, the Sprague–Dawley (Taconic) and Wistar-Unilever (HsdCpb:Wu) (Envigo) strains, and custom-formulated Zn-deficient (3–4 ppm Zn) and Zn-sufficient (∼60 ppm Zn) diets from Harlan Teklad.

    Techniques: Labeling, Staining, Microscopy, Binding Assay, Activity Assay, Real-time Polymerase Chain Reaction

    Prostate miRNA expression profiling by the nanoString nCounter rat miRNA assay in Sprague–Dawley rats ( A – C ) and Wistar-Unilever rats ( D and E ) growing from young adult into middle age on a Zn-deficient diet or a Zn-sufficient diet. ( A ) Bar plot showing fold-change of 14 miRNAs up-regulated in Zn-deficient middle-aged vs. Zn-deficient young-adult prostates that are similarly up-regulated in human PCa compared with 11 such miRNAs up-regulated in Zn-sufficient middle-aged vs. Zn-sufficient young-adult prostates (cutoff: fold change ≥1.4, P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Human-like hyperplastic prostate with low ZIP1 induced solely by Zn deficiency in rats

    doi: 10.1073/pnas.1813956115

    Figure Lengend Snippet: Prostate miRNA expression profiling by the nanoString nCounter rat miRNA assay in Sprague–Dawley rats ( A – C ) and Wistar-Unilever rats ( D and E ) growing from young adult into middle age on a Zn-deficient diet or a Zn-sufficient diet. ( A ) Bar plot showing fold-change of 14 miRNAs up-regulated in Zn-deficient middle-aged vs. Zn-deficient young-adult prostates that are similarly up-regulated in human PCa compared with 11 such miRNAs up-regulated in Zn-sufficient middle-aged vs. Zn-sufficient young-adult prostates (cutoff: fold change ≥1.4, P

    Article Snippet: We used two rat strains, the Sprague–Dawley (Taconic) and Wistar-Unilever (HsdCpb:Wu) (Envigo) strains, and custom-formulated Zn-deficient (3–4 ppm Zn) and Zn-sufficient (∼60 ppm Zn) diets from Harlan Teklad.

    Techniques: Expressing