Journal: Oncotarget

Article Title: The potent tumor suppressor miR-497 inhibits cancer phenotypes in nasopharyngeal carcinoma by targeting ANLN and HSPA4L

doi:

Figure Lengend Snippet: A. mRNA expression levels of ANLN (left) and HSPA4L (right) in NNE tissues ( n = 11) and primary NPC tissues ( n = 18), determined by quantitative RT-PCR. Expression levels of target genes ( ANLN and HSPA4L ) were normalized against the corresponding levels of GAPDH . P -values were calculated using Student's t -test (* P < 0.05). B. Formalin-fixed and paraffin-embedded biopsies of nasopharyngeal tissues were obtained from patients with chronic nasopharyngitis (NNE) or NPC. Expression of ANLN (left) and HSPA4L (right) was assessed by immunoperoxidase staining (brown). Original magnifications are 100× and 200 ×. Scale bar represents 50 μm. C. Graphs represent average and SD of IHC scores for NNE ( n = 6) and NPC tissues ( n = 6). P -values of differences between NPC and NNE tissues were calculated using the Mann–Whitney U test (** P < 0.01).

Article Snippet: Small interfering RNA (siRNA) for candidate target genes ANLN (SDO-1006-1005899, GENE_ID54443) and HSPA4L (SDO-1006-1071379, GENE_ID22824), as well as negative control siRNA (SN-1001), were synthesized by BIONEER (Daejeon, South Korea).

Techniques: Expressing, Quantitative RT-PCR, Immunoperoxidase Staining, MANN-WHITNEY

Journal: Oncotarget

Article Title: The potent tumor suppressor miR-497 inhibits cancer phenotypes in nasopharyngeal carcinoma by targeting ANLN and HSPA4L

doi:

Figure Lengend Snippet: A. Expression levels of ANLN (left) and HSPA4L (right) in HK1 cells ( n = 4) after transfection with miR-497 mimic. Expression levels of target genes ( ANLN and HSPA4L ) were determined by quantitative RT-PCR and normalized against the corresponding levels of GAPDH . B. Protein expression levels of ANLN (left) and HSPA4L (right) in HK1/EBV cells, determined by ICC. Scale bar represents 50 μm. Numbers of positively staining cells and total cell number (determined by DAPI staining of nuclei) were analyzed using the ImageJ software, and the percentage of positively stained cells was calculated for each sample ( n = 4) in three areas. P -values were calculated using the Student's t -test (* P < 0.05, ** P < 0.01, *** P < 0.001).

Article Snippet: Small interfering RNA (siRNA) for candidate target genes ANLN (SDO-1006-1005899, GENE_ID54443) and HSPA4L (SDO-1006-1071379, GENE_ID22824), as well as negative control siRNA (SN-1001), were synthesized by BIONEER (Daejeon, South Korea).

Techniques: Expressing, Transfection, Quantitative RT-PCR, Staining, Software

Journal: Oncotarget

Article Title: The potent tumor suppressor miR-497 inhibits cancer phenotypes in nasopharyngeal carcinoma by targeting ANLN and HSPA4L

doi:

Figure Lengend Snippet: A. Cell proliferation was determined by MTT assay every 24 h for 96 h after transfection of CNE1 cells with ANLN siRNA (left) or HSPA4L siRNA (right) ( n = 5). B. Apoptotic cells were detected by flow cytometry. CNE1 cells were transfected with negative control siRNA, ANLN siRNA or HSPA4L siRNA, for 72 h, and then stained with annexin V-FITC and PI and subjected to flow cytometry ( n = 3). C. Cell migration assay in CNE1 cells transfected with negative control siRNA, ANLN siRNA, or HSPA4L siRNA. At 72 h after transfection, harvested cells were seeded in a migration chamber and cultured for 24 h. Migratory cells were stained, photographed under a microscope, and quantitatively analyzed ( n = 3). Data are shown as means ± standard deviation. P -values were calculated using the Student's t -test (* P < 0.05, ** P < 0.01, *** P < 0.001) compared with the control.

Article Snippet: Small interfering RNA (siRNA) for candidate target genes ANLN (SDO-1006-1005899, GENE_ID54443) and HSPA4L (SDO-1006-1071379, GENE_ID22824), as well as negative control siRNA (SN-1001), were synthesized by BIONEER (Daejeon, South Korea).

Techniques: MTT Assay, Transfection, Flow Cytometry, Negative Control, Staining, Cell Migration Assay, Migration, Cell Culture, Microscopy, Standard Deviation, Control