Journal: Cardiovascular Diabetology

Article Title: Endothelial biomarkers and platelet reactivity on ticagrelor versus clopidogrel in patients after acute coronary syndrome with and without concomitant type 2 diabetes: a preliminary observational study

doi: 10.1186/s12933-022-01685-4

Figure Lengend Snippet: Platelet reactivity, sP-selectin and endothelial biomarkers in patients on DAPT with ticagrelor and clopidogrel stratified by diabetes status

Article Snippet: Additionally, asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) were measured in a subgroup of the study subjects by ELISA (Immundiagnostik AG, Bernsheim, Germany).

Techniques:

Journal: Animals : an Open Access Journal from MDPI

Article Title: Biomarkers of Kidney Disease in Horses: A Review of the Current Literature

doi: 10.3390/ani12192678

Figure Lengend Snippet: Flow diagram of the literature search for studies on biomarkers on equine kidney disease. SDMA—symmetric dimethylarginine; NGAL—neutrophil gelatinase-associated lipocalin. Searches were always in combination with the search word horse or equine.

Article Snippet: 1 , Lo, H.C., Winter, J.C., Merle, R., Gehlen, H. Symmetric dimethylarginine and renal function analysis in horses with dehydration. Equine Vet J. 10 June 2021 [ ]. , DLD SDMA ELISA kit (DLD Diagnostika GmbH) (validated by [ ]) , 41 horses with dehydration , 1 with AKI for 48 h, 1 with AKI for 12 h , Good correlation with creatinine, not with urea , Dehydration severity AKI: SDMA elevated 2 cases with normal creatinine but with elevated SDMA (subclinical AKI?) Lower in survivors than non-survivors, but not statistical , Phase II , n/a , Dehydration , Limited AKI cases.

Techniques:

Journal: Animals : an Open Access Journal from MDPI

Article Title: Biomarkers of Kidney Disease in Horses: A Review of the Current Literature

doi: 10.3390/ani12192678

Figure Lengend Snippet: List of studies on symmetric dimethylarginine (SDMA) concentrations in blood in horses and its analytical and clinical characteristics.

Article Snippet: 1 , Lo, H.C., Winter, J.C., Merle, R., Gehlen, H. Symmetric dimethylarginine and renal function analysis in horses with dehydration. Equine Vet J. 10 June 2021 [ ]. , DLD SDMA ELISA kit (DLD Diagnostika GmbH) (validated by [ ]) , 41 horses with dehydration , 1 with AKI for 48 h, 1 with AKI for 12 h , Good correlation with creatinine, not with urea , Dehydration severity AKI: SDMA elevated 2 cases with normal creatinine but with elevated SDMA (subclinical AKI?) Lower in survivors than non-survivors, but not statistical , Phase II , n/a , Dehydration , Limited AKI cases.

Techniques: Comparison, Biomarker Discovery, Enzyme-linked Immunosorbent Assay, High Throughput Screening Assay, Concentration Assay, Targeted Proteomics, Mass Spectrometry, Chromatography, Spectroscopy

Journal: Animals : an Open Access Journal from MDPI

Article Title: Biomarkers of Kidney Disease in Horses: A Review of the Current Literature

doi: 10.3390/ani12192678

Figure Lengend Snippet: Overview of molecular and physiological information on the identified kidney biomarkers.

Article Snippet: 1 , Lo, H.C., Winter, J.C., Merle, R., Gehlen, H. Symmetric dimethylarginine and renal function analysis in horses with dehydration. Equine Vet J. 10 June 2021 [ ]. , DLD SDMA ELISA kit (DLD Diagnostika GmbH) (validated by [ ]) , 41 horses with dehydration , 1 with AKI for 48 h, 1 with AKI for 12 h , Good correlation with creatinine, not with urea , Dehydration severity AKI: SDMA elevated 2 cases with normal creatinine but with elevated SDMA (subclinical AKI?) Lower in survivors than non-survivors, but not statistical , Phase II , n/a , Dehydration , Limited AKI cases.

Techniques: Biomarker Discovery, Functional Assay, Methylation, Generated, Analogues, Transduction, Concentration Assay, Produced, Protease Inhibitor, Modification, Bacteria, Infection, Activation Assay, Filtration, Clinical Proteomics

Journal: Parasites & Vectors

Article Title: Evaluation of urinary podocin and nephrin as markers of podocyturia in dogs with leishmaniosis

doi: 10.1186/s13071-024-06510-3

Figure Lengend Snippet: Blood analysis of healthy dogs and dogs with leishmaniosis

Article Snippet: In addition, symmetric-dimethylarginine (SDMA) was measured with a canine SDMA ELISA test (Eurolyser Diagnostica GmbH, Salzburg, Austria).

Techniques:

Journal: Parasites & Vectors

Article Title: Evaluation of urinary podocin and nephrin as markers of podocyturia in dogs with leishmaniosis

doi: 10.1186/s13071-024-06510-3

Figure Lengend Snippet: Various serum and urinary parameters of healthy dogs and dogs with leishmaniosis based on IRIS staging

Article Snippet: In addition, symmetric-dimethylarginine (SDMA) was measured with a canine SDMA ELISA test (Eurolyser Diagnostica GmbH, Salzburg, Austria).

Techniques:

Journal: Parasites & Vectors

Article Title: Evaluation of urinary podocin and nephrin as markers of podocyturia in dogs with leishmaniosis

doi: 10.1186/s13071-024-06510-3

Figure Lengend Snippet: Correlations between urinary podocin and nephrin concentrations and various renal/urinary markers in the two groups

Article Snippet: In addition, symmetric-dimethylarginine (SDMA) was measured with a canine SDMA ELISA test (Eurolyser Diagnostica GmbH, Salzburg, Austria).

Techniques:

Journal: Parasites & Vectors

Article Title: Evaluation of urinary podocin and nephrin as markers of podocyturia in dogs with leishmaniosis

doi: 10.1186/s13071-024-06510-3

Figure Lengend Snippet: Correlations between urinary podocin and nephrin concentrations and various renal/urinary markers according to IRIS stages

Article Snippet: In addition, symmetric-dimethylarginine (SDMA) was measured with a canine SDMA ELISA test (Eurolyser Diagnostica GmbH, Salzburg, Austria).

Techniques:

Journal: Parasites & Vectors

Article Title: Evaluation of urinary podocin and nephrin as markers of podocyturia in dogs with leishmaniosis

doi: 10.1186/s13071-024-06510-3

Figure Lengend Snippet: Correlations between the uNeC and uPoC and various renal/urinary markers in the two groups

Article Snippet: In addition, symmetric-dimethylarginine (SDMA) was measured with a canine SDMA ELISA test (Eurolyser Diagnostica GmbH, Salzburg, Austria).

Techniques:

Journal: Parasites & Vectors

Article Title: Evaluation of urinary podocin and nephrin as markers of podocyturia in dogs with leishmaniosis

doi: 10.1186/s13071-024-06510-3

Figure Lengend Snippet: Correlations between the uNeC and uPoC and various renal/urinary markers according to IRIS stages

Article Snippet: In addition, symmetric-dimethylarginine (SDMA) was measured with a canine SDMA ELISA test (Eurolyser Diagnostica GmbH, Salzburg, Austria).

Techniques:

Journal: PLoS ONE

Article Title: The renal transport protein OATP4C1 mediates uptake of the uremic toxin asymmetric dimethylarginine (ADMA) and efflux of cardioprotective L-homoarginine

doi: 10.1371/journal.pone.0213747

Figure Lengend Snippet: Concentration-dependent inhibition of OATP4C1-mediated L-homoarginine (2 μ M) net uptake by uremic toxins ADMA (A) and SDMA (B). The calculated net uptake values were used to determine IC 50 values. Data are expressed as means ± SEM. Experiments were performed on two separate days with n = 3 (n = 6). *P < 0.05; ***P < 0.0001; one-way ANOVA Dunnett`s multiple comparison test.

Article Snippet: Unlabeled ADMA, SDMA and L-arginine were from Enzo Life Sciences GmbH (Lörrach, Germany) and unlabeled L-homoarginine was obtained from Acros Organics (New Jersey, U.S.A.).

Techniques: Concentration Assay, Inhibition

Journal: PLoS ONE

Article Title: Dysregulated Hepatic Methionine Metabolism Drives Homocysteine Elevation in Diet-Induced Nonalcoholic Fatty Liver Disease

doi: 10.1371/journal.pone.0136822

Figure Lengend Snippet: (A) Heat map representation of substrates and methylated products of major SAM-derived transmethylation reactions (PEMT, GNMT, GAMT and PRMT) with HFHC diet for 52 weeks. (B) Levels of glycine, a substrate for GNMT, were decreased (p< 0.01) while the product sarcosine did not change between chow and HFHC group. (C) The gene expression of Gnmt , the most abundant methyltranserase in the liver, decreased significantly but only approached significance at the protein level (p = 0.06). (D) Levels of PE, the substrate for PEMT, was significantly reduced (p< 0.01) while PC trended down, resulting in a significant PC/PE ratio (p< 0.01), which suggests increase in PEMT activity There were no significant changes in the concentrations of guanidinoacetate and creatine, the substrate and product for GAMT, respectively. (E) The methylated products of PRMT activity, MMA and ADMA, were decreased significantly (p< 0.01 and p< 0.05) while the decrease in SDMA approached significance (p = 0.07). (F) The gene expression of Prmt1 , mainly responsible for the methylation of MMA and ADMA, decreased (p< 0.01) but the protein levels of PRMT1 increased significantly (p< 0.05). (G) Circulating ADMA was increased toward significance (p = 0.06), suggesting increased export to the circulation. Data are represented as mean ± SEM. Legend: arginine (Arg); asymmetric dimethylarginine (ADMA); creatine (Crea); glycine (Gly); glycine N-methyltransferase (GNMT); guanidinoacetate (GAA); guanidinoacetate methyltransferase (GAMT); homocysteine (Hcy); monomethylarginine (MMA); phosphatidylcholine (PC); phosphatidylethanolamine (PE); phosphatidylethanolamine methyltransferase (PEMT); protein arginine methyltransferase (PRMT); s-adenosylhomocysteine (SAH); s-adenosylmethionine (SAM); sarcosine (Sar); symmetric dimethylarginine (SDMA).

Article Snippet: Asymmetric-dimethylarginine (ADMA) and symmetric-dimethylarginine (SDMA) were purchased from Axxora LLC (San Diego, CA).

Techniques: Methylation, Derivative Assay, Expressing, Activity Assay