Journal: Advanced Science

Article Title: Transcriptome Landscape of Cancer‐Associated Fibroblasts in Human PDAC

doi: 10.1002/advs.202415196

Figure Lengend Snippet: Cell–cell interactions between CAF subtypes and myeloid cells. A) Boxplot showing the percentage of MKI67 + cells in each cell type within tumor tissues. Each dot corresponds to each sample. B) Boxplot showing the frequency of each CAF subtype in tumors. Each dot corresponds to each sample. C) Heatmap showing the Spearman correlation coefficient between the abundance of CAF subtypes and the proliferation percent of other cells in tumors. Correlation test p values are indicated, *** p < 0.001, ** p < 0.01, and * p < 0.05. D) Cell–cell interaction network showing interactions between CAF subtypes and other cells in tumors. The dot color indicates the cell type, the dot size indicates the interaction number of a given cell type, and the line thickness indicates the interaction number of a given cell type pair. E) Bar plots showing the interaction number of a given cell type. F) Dot plot showing GO terms of ligands and receptors within specific myeloid cells‐CAFs interactions and specific lymphocytes‐CAFs interactions. G) Dot plot showing the expression level of ligand‐receptor gene pair related with chemotaxis and cytokine within myeloid cell‐CAF interactions. The dot color and size indicate the expression levels and statistical significance, respectively. Red words indicate the ligands (row) expressed in the corresponding cell type (column), and blue words indicate the receptors (row) expressed in the corresponding cell type (column). H) Bar plot showing the fold change of neutrophil migration obtained following the addition of the CAFs/NFs or the CM derived from CAFs/NFs in lower chamber after 1 h. For each group, n = 7 biological replicates. Data are shown as mean value ± SD. One‐way ANOVA p values are calculated. * p < 0.05 and *** p < 0.001. I) Neutrophils were treated with or without CXCL4 inhibitor Plerixafor (25 µM). Neutrophil migration assay was performed by adding control medium or culture medium of CAFs into lower champers, or seeding CAFs into lower champers with or without Plerixafor (25 µM). Bar plot showing the fold change of neutrophil migration after 1 h. For each group, n = 7 biological replicates. Data are shown as mean value ± SD. One‐way ANOVA p values are calculated. ** p < 0.01, **** p < 0.0001, and ns, not significant. J) Cartoon depicting CXCL12‐CXCR4 as a major interaction axis between CAFs and myeloid cells.

Article Snippet: Primary antibodies: CXCL12 (Cat# 17402‐1‐AP, Proteintech), PDPN (Cat# ab236529, Abcam), CK18 (Cat# 10830‐1‐AP, Proteintech), ISG15 (Cat# 15981‐1‐AP, Proteintech), α‐SMA (Cat# ab5694, Abcam), COLL1A1 (Cat# 72026, CST), HLA‐DR (Cat# ab20181, Abcam), CD74 (Cat# ab108393, Abcam), FTL (Cat# 10727‐1‐AP, Proteintech), CDCP1 (Cat# ab252947, Abcam), HIF‐1α (Cat# 20960‐1‐AP, Proteintech), LUM (Cat# ab168343, Abcam), CD3 (Cat# ab5690, Abcam), CD19 (Cat# ab31947, Abcam), CD16 (Cat# 16559‐1‐AP, Proteintech), TPSAB1 (Cat# ab151757, Abcam).

Techniques: Expressing, Chemotaxis Assay, Migration, Derivative Assay, Control

Journal: Nature Cell Biology

Article Title: Peritumoural adipose tissue drives immune evasion in colorectal cancer via adipose–mesenchymal transformation

doi: 10.1038/s41556-026-01885-0

Figure Lengend Snippet: a . Representative images of MC38-OVA (left) and tumour growth (right) in C57BL/6J mice ( n = 5). b . Representative images of CT26 tumours (left) and tumour growth (right) in BALB/c mice ( n = 5). c . Schematic diagram illustrating the working principle of ATS-GNP adipocytolysis. ATS-GNP, containing a CaCO 3 core, is receptor-mediated for endocytosis. Upon reaching the acidic environment, ATS-GNP releases CO 2 gas, disrupting the adipose cell membrane. d . Tumour growth of MC38 tumours in C57BL/6J, BALB/c-Nude (left), and NSG (right) mice ( n = 5). e . Representative images of E0771 tumours at day 16 of the experiment in C57BL/6J mice following removal of PAT ( n = 5). f . Tumour weights of E0771 tumours at day 16 in C57BL/6J mice with the removal of PAT ( n = 5). g . Representative flow cytometry plots of CD45 + cells gated on live cells in MC38-OVA tumours. h . Violin plots showing the expression of CXCR7 across all cell types in CRC patients, analysed using the Kruskal-Wallis test. i . Editing strategy for constructing Cxcl12 fl/fl cKO mouse. j . Experimental design for constructing Control and Cxcl12 fl/fl cKO mice bearing MC38-OVA tumours near PAT. k . Western blot analysis for verifying the knockout efficiency of Cxcl12 in PAT of Control mice and Cxcl12 fl/fl cKO mice. l . RT-qPCR analysis for verifying the Cxcl12 knockout efficiency in liver, spleen, uterus, and tumour tissues from Control mice and Cxcl12 fl/fl cKO mice ( n = 6). Data represent ≥ 3 independent experiments. P -values were calculated using two-way ANOVA with Tukey’s correction for multiple comparisons ( a -right, b -right, and d ), two-way ANOVA with Bonferroni’s correction for multiple comparisons ( f ), or a two-sided, unpaired Student’s t -test ( l ). Graphs display mean ± SD ( a , b , d , f , l ). Panels created with BioRender : c , i and j , Huaiqiang, J. https://biorender.com/cy7rgjm (2026).

Article Snippet: For drug treatment, a CXCL12 neutralizing antibody (Merck Millipore) and an anti-mouse PD-1 antibody (Bio X Cell) were administered via intraperitoneal injection.

Techniques: Membrane, Flow Cytometry, Expressing, Control, Western Blot, Knock-Out, Quantitative RT-PCR

Journal: Nature Cell Biology

Article Title: Peritumoural adipose tissue drives immune evasion in colorectal cancer via adipose–mesenchymal transformation

doi: 10.1038/s41556-026-01885-0

Figure Lengend Snippet: a , Cell–cell communication analysis based on ligand–receptor interactions (top six) between stromal cells and lymphocytes in tVAT (left) and a comparison between tVAT, dVAT and tumour (right). b , Marked CXCL12–CXCR4 interactions among CD8 + T cells, CD4 + T cells, B cells, plasma cells and stromal cell populations in tVAT, dVAT and tumour. The width of the lines represents the probability of communication. c , Violin plots showing the expression of CXCL12 (top) and CXCR4 (bottom) across all cell types in patients with CRC. d , Violin plots comparing the expression of CXCL12 in dVAT versus tVAT (top) and tumour versus tVAT (bottom) in patients with CRC, analysed using a two-sided Wilcoxon test. e , Experimental design for the PAT C57BL/6J mouse model treated with IgG or anti-CXCL12 antibody (left), and representative MC38 tumour images at day 16 of the experiment (right) ( n = 5). f , Tumour growth (left) and tumour weights (right) of MC38 tumours at day 16 of the experiment in C57BL/6J mice ( n = 5). g , Representative MC38 tumour images (left) and tumour growth (right) of experiments in control and Cxcl12 fl/fl cKO mice ( n = 6). h , Tumour weights of MC38 tumours in control and Cxcl12 fl/fl cKO mice at day 16 of the experiment ( n = 6). i , Flow cytometry analysis of the infiltration of various CXCR4 + immune cells in MC38 tumours in Control and Cxcl12 fl/fl cKO mice ( n = 6). j , Schematic diagram of the chemotaxis assay using T cells as ‘sensors’ and conditional medium as a ‘sink’ (left), and the aggregated trajectories of control or CXCL12-induced T cells migrating for 1 h (right). k , Quantitative analysis of CXCR4 + CD45.1 + T cells in MC38 tumours with and without removal of PAT or contralateral inguinal adipose tissue (control) by flow cytometry ( n = 5). Data represent ≥3 independent experiments. Statistical significance was assessed by a two-sided permutation test ( a ), two-sided unpaired Student’s t -test ( f right, h and i ), one-way analysis of variance (ANOVA) with Tukey’s correction for multiple comparisons ( k right) or two-way ANOVA ( f left and g right). Graphs display mean ± s.d. ( f – i , k ). Panels created with BioRender : e and k , Huaiqiang, J. https://biorender.com/ovq2e39 (2026).

Article Snippet: For drug treatment, a CXCL12 neutralizing antibody (Merck Millipore) and an anti-mouse PD-1 antibody (Bio X Cell) were administered via intraperitoneal injection.

Techniques: Comparison, Clinical Proteomics, Expressing, Control, Flow Cytometry, Chemotaxis Assay

Journal: Nature Cell Biology

Article Title: Peritumoural adipose tissue drives immune evasion in colorectal cancer via adipose–mesenchymal transformation

doi: 10.1038/s41556-026-01885-0

Figure Lengend Snippet: a . UMAP plot of VAT-associated stromal cells in tVAT and dVAT from CRC patients. The 8 clusters, labelled by inferred cell types, are denoted by colour. b . Dot plot showing RNA expression of marker genes used to define pAC, adCAF, and APC subclusters. Circle size represents the log-normalized P -value, while colour intensity indicates the log-transformed mean expression of marker genes. c . Sorting strategy for isolating adCAFs from mouse PAT by flow cytometry. d . RNA sequencing analysis of adCAF markers in sorted adCAF-enriched stromal cells and non-adCAF stromal cells ( n = 4). Statistical significance was assessed using a two-sided, unpaired Student’s t -test. e . RNA sequencing analysis of adipogenesis markers in sorted adCAF-enriched stromal cells and non-adCAF stromal cells ( n = 4). Statistical significance was assessed using a two-sided, unpaired Student’s t -test. f . Representative images of the morphology of sorted adCAF-enriched stromal cells and non-adCAF stromal cells. Scale bar = 500 μm. g . Cross-tissue interactions analysis based on ligand-receptor pairs between adCAFs and tumour cells (left) and a comparison between tVAT and dVAT (right). A two-sided permutation test was used to determine the significance of pathways. h . Western blot analysis of CXCL12 protein expression in sorted adCAF-enriched stromal cells and non-adCAF stromal cells ( n = 3). The data are presented as a box-and-whisker graph (bounds of box: first to third quartile, bottom and top line: minimum to maximum, central line: median) for ( d - e ).

Article Snippet: For drug treatment, a CXCL12 neutralizing antibody (Merck Millipore) and an anti-mouse PD-1 antibody (Bio X Cell) were administered via intraperitoneal injection.

Techniques: RNA Expression, Marker, Transformation Assay, Expressing, Flow Cytometry, RNA Sequencing, Comparison, Western Blot, Whisker Assay

Journal: Nature Cell Biology

Article Title: Peritumoural adipose tissue drives immune evasion in colorectal cancer via adipose–mesenchymal transformation

doi: 10.1038/s41556-026-01885-0

Figure Lengend Snippet: a , UMAP of all stromal cells in tVAT, dVAT, tumour and normal from patients with CRC, with ten clusters labelled by inferred cell types. Major lineages included ASCs, pACs, CAFs, pericytes (PCs) and mesothelial cells (Mesos). b , Heatmap displaying the distribution of eight stromal cell subtypes across different tissue types. c , UMAP of eight subsets of VAT-associated stromal cells in tVAT and dVAT from patients with CRC, including ASCs, pACs and adCAFs. d , Beeswarm plot showing the distribution and abundance of VAT-associated stromal cell types in Nhoods between tVAT and dVAT. e , Stack plot displaying the abundance of the eight VAT-associated stromal cell subsets in dVAT and tVAT. f , Heatmap showing the RNA expression of various marker genes in VAT-associated stromal cell types, including ASC/pAC markers, CAF markers, cytokines and stromal markers. g , Representative multiplex immunofluorescence images showing the presence of adCAFs in tVAT samples from patients with CRC. Scale bar, 10 μm. DAPI, 4,6-diamidino-2-phenylindole. h , Relative expression levels of multiple cytokines and protumoural factors in adCAF-enriched stromal cells ( n = 4) and non-adCAF stromal cells ( n = 4) derived from the PAT of mice xenograft models. The data are presented as a box-and-whisker graph (bounds of box show first to third quartile, bottom and top line show minimum to maximum and the central line shows the median). i , Cell–cell communication analysis based on ligand–receptor pairs (top six) between adCAFs and lymphocytes in tVAT (left) and a comparison between tVAT and dVAT (right). j , RT–qPCR (left) and ELISA (right) detecting the RNA expression and protein secretion of CXCL12 in sorted adCAF-enriched stromal cells and non-adCAF stromal cells from PAT of mice. Data represent ≥3 independent experiments. All data are shown as mean ± s.d. and statistical significance was assessed by a two-sided, unpaired Wilcoxon test ( h ), two-sided permutation test ( i ) and Student’s t -test ( j ).

Article Snippet: For drug treatment, a CXCL12 neutralizing antibody (Merck Millipore) and an anti-mouse PD-1 antibody (Bio X Cell) were administered via intraperitoneal injection.

Techniques: RNA Expression, Marker, Multiplex Assay, Immunofluorescence, Expressing, Derivative Assay, Whisker Assay, Comparison, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: Nature Cell Biology

Article Title: Peritumoural adipose tissue drives immune evasion in colorectal cancer via adipose–mesenchymal transformation

doi: 10.1038/s41556-026-01885-0

Figure Lengend Snippet: a . UMAP of all cells in tVAT and dVAT from 5 CRC patients, with 16 clusters labelled by inferred cell types. Major lineages included various immunocytes, VAT-associated stromal cells, adipocytes (ACs), endothelial cells (ECs), pericytes (PCs) and mesothelial cells (Mesos). b . Heatmap of representative marker genes across all cell populations. c . UMAP of 6 subsets of ACs in tVAT and dVAT from 5 CRC patients. d . Beeswarm plot showing the distribution and abundance of ACs in Nhoods between tVAT and dVAT. e . Box plot to compare the abundance of ACs in dVAT ( n = 5) and tVAT ( n = 5). P -values were calculated using a two-side Wilcoxon test. f . Cell-cell communication analysis based on ligand-receptor interactions (top 3) between CD8 + T cells and adipocytes, and between CD8 + T cells and VAT-associated stromal cells in tVAT. A two-sided permutation test was used to determine the significance of pathways. Only significant ligand-receptor pairs ( P < 0.05) are shown. Dot size encodes the interaction score, and colour scale indicates the communication probability. g . Violin plots showing the expression of CXCL12, VAT-associated markers, and CAF-associated markers in adipocytes and VAT-associated stromal cells from CRC patients. h . UMAP of 8 subsets of VAT-associated stromal cells in tVAT and dVAT from 5 CRC patients, including APCs, pACs and adipocyte-derived cancer-associated fibroasts (adCAFs). The 8 clusters, labelled by inferred cell types, are denoted by colour. i . Beeswarm plot showing the distribution and abundance of VAT-associated stromal cell types in Nhoods between tVAT ( n = 5) and dVAT ( n = 5). j . Box plot to compare the abundance of VAT-associated stromal cells in dVAT and tVAT. P -values were calculated using a two-side Wilcoxon test. k . Heatmap showing the RNA expression patterns of representative marker genes across all cell populations, including CAF markers, stromal markers, APC/pAC markers, white/brown/beige fat markers, and cytokines and growth markers. The data are presented as a box-and-whisker graph (bounds of box: first to third quartile, bottom and top line: minimum to maximum, central line: median) for ( e , j ).

Article Snippet: For drug treatment, a CXCL12 neutralizing antibody (Merck Millipore) and an anti-mouse PD-1 antibody (Bio X Cell) were administered via intraperitoneal injection.

Techniques: Marker, Expressing, Derivative Assay, RNA Expression, Whisker Assay

Journal: Nature Cell Biology

Article Title: Peritumoural adipose tissue drives immune evasion in colorectal cancer via adipose–mesenchymal transformation

doi: 10.1038/s41556-026-01885-0

Figure Lengend Snippet: a , Experimental design for constructing Control and Mdk DTR cKO mice bearing MC38 tumours near PAT, followed by αPD-1 therapy. b , c , Representative MC38 tumour images ( b ), tumour weights ( c left) and tumour growth ( c right) in control and Mdk DTR cKO mice treated with IgG or anti-PD-1 ( n = 6). d , Flow cytometry analysis of the infiltration of immunocytes, including T cells, CD4 + T cells, CD8 + T cells, and tumour-specific CD8 + T cells, CXCR4 + immunocytes, CXCR4 + T cell, CXCR4 + CD4 + T cell, CXCR4 + CD8 + T cell and CXCR4 + tumour-specific T cell in MC38-OVA tumours from the four treatment groups ( n = 6). e , f , Representative MC38 tumour images ( e ) and tumour weights ( f ) of the experiment in mice treated with anti-CXCL12 and/or anti-PD-1 ( n = 5). g , Representative MRI image of CRC tumour and corresponding tVAT area region of CR and non-CR patients pre- and post-immuno-chemoradiotherapy. The yellow area represents the tVAT area, whereas the red area denotes the tumour region. Note that the mass visible in the intestinal lumen (top right) is faecal material. h , Pre-treatment tVAT area difference based on 3D Slicer between CR ( n = 30) and non-CR ( n = 37) patients. The data are presented as a box-and-whisker graph (bounds of box show first to third quartile, bottom and top line show minimum to maximum and the central line shows the median). i , ROC plot of response predicting ability of pre-treatment PAT area in immuno-chemoradiotherapy of proficient mismatch repair patients with CRC, compared with conventional indexes, including CPS, TPS, CEA and CA199 ( n = 67) with optimal cutoff. j , Comparison of pCR ratio in tVAT high and low group according to the optimal cutoff. k , Graphical abstract depicting how tumours reshape the stromal environment in tVAT and how tVAT competes for immunocytes from the tumour to promote immune escape. Data represent ≥3 independent experiments. Statistical significance was assessed using a two-sided, unpaired Student’s t -test ( d , h ), one-way ANOVA with Tukey’s correction for multiple comparisons ( c left, f ) or two-way ANOVA with Tukey’s correction for multiple comparisons ( c right). Graphs display mean ± s.d. ( c , d , f , h ). Panels created with BioRender : a and k , Huaiqiang, J. https://biorender.com/e5jwcye (2026).

Article Snippet: For drug treatment, a CXCL12 neutralizing antibody (Merck Millipore) and an anti-mouse PD-1 antibody (Bio X Cell) were administered via intraperitoneal injection.

Techniques: Control, Flow Cytometry, Whisker Assay, Comparison

Journal: Nature Cell Biology

Article Title: Peritumoural adipose tissue drives immune evasion in colorectal cancer via adipose–mesenchymal transformation

doi: 10.1038/s41556-026-01885-0

Figure Lengend Snippet: a . Editing strategy for constructing Mdk DTR mouse. b . Flow cytometry representative plots and bar graphs for verifying the elimination efficiency of adCAFs (FAP + PDGFRB + MDK + stromal cells) in PAT of Control mice and Mdk DTR cKO mice. c . Experimental design for combination therapy with αCXCL12 and αPD-1 in mice bearing MC38-OVA tumours near PAT. d . MC38 tumour weights of the experiment in mice treated with αCXCL12 and/or αPD-1 ( n = 6). e . Flow cytometry analysis of the infiltration of immunocytes, including T cells, CD4 + T cells, CD8 + T cells, and tumour-specific CD8 + T cells in MC38-OVA tumours from the 4 treatment groups ( n = 6). f . Flow cytometry analysis of the infiltration of CXCR4 + immunocytes, including CXCR4 + T cell, CXCR4 + CD4 + T cell and CXCR4 + CD8 + T cell in MC38-OVA tumours from the 4 treatment groups ( n = 6). g . Boxplot of difference of tVAT area in CR and non-CR patients with T3 or T4 stage separately. The data are presented as a box-and-whisker graph (bounds of box: first to third quartile, bottom and top line: minimum to maximum, central line: median). h . ROC plot of prediction ability of tVAT area in patients with T3 or T4 stage separately. Data represent ≥ 3 independent experiments. P -values were calculated using a two-sided, unpaired Student’s t -test ( b , e - g ) and two-way ANOVA with Tukey’s correction for multiple comparisons ( d ). Graphs display mean ± SD ( b , d , e - g ). Panels created with BioRender : a and c , Huaiqiang, J. https://biorender.com/33r2gmm (2026).

Article Snippet: For drug treatment, a CXCL12 neutralizing antibody (Merck Millipore) and an anti-mouse PD-1 antibody (Bio X Cell) were administered via intraperitoneal injection.

Techniques: Flow Cytometry, Control, Whisker Assay

Journal: Nature Cell Biology

Article Title: Peritumoural adipose tissue drives immune evasion in colorectal cancer via adipose–mesenchymal transformation

doi: 10.1038/s41556-026-01885-0

Figure Lengend Snippet: a . Experimental design for combination therapy with AMD3100 and αPD-1 in mice bearing MC38-OVA tumours near subcutaneous PAT. b . Representative MC38 tumour images of the mice treated with AMD3100 and/or αPD-1 ( n = 5). c . Representative MC38 tumour weights of the mice treated with AMD3100 and/or αPD-1 ( n = 5). d . Representative MC38 tumour growth of the mice treated with AMD3100 and/or αPD-1 ( n = 5). e . Representative MC38 tumour images in MC38-OVA caecal orthotopic tumour-bearing mice treated with AMD3100 and/or αPD-1 ( n = 5). f . Representative MC38 tumour weights in MC38-OVA caecal orthotopic tumour-bearing mice treated with AMD3100 and/or αPD-1 ( n = 5). g . Representative MC38 Bioluminescence Images in MC38-OVA caecal orthotopic tumour-bearing mice treated with AMD3100 and/or αPD-1 ( n = 5). h . Representative MC38 tumour images in MC38-OVA caecal orthotopic tumour-bearing mice treated with αCXCL12 and/or αPD-1 ( n = 5). i . Representative MC38 tumour weights in MC38-OVA caecal orthotopic tumour-bearing mice treated with αCXCL12 and/or αPD-1 ( n = 5). j . Representative MC38 Bioluminescence Images in MC38-OVA caecal orthotopic tumour-bearing mice treated with αCXCL12 and/or αPD-1 ( n = 5). k . Flow cytometry analysis of the infiltration of immunocytes, including T cells, CD4 + T cells, CD8 + T cells, and tumour-specific CD8 + T cells in MC38-OVA tumours from 4 treatment groups ( n = 5). l . Flow cytometry analysis of the infiltration of CXCR4 + immunocytes, including CXCR4 + T cell, CXCR4 + CD4 + T cell and CXCR4 + CD8 + T cell in MC38-OVA tumours from 4 treatment groups ( n = 5). Data represent ≥ 3 independent experiments. P -values were calculated using a two-sided, unpaired Student’s t -test ( c , f , i , k , l ) and two-way ANOVA with Tukey’s correction for multiple comparisons ( d ). Graphs display mean ± SD ( c - d , f , i , k - l ). Panel created with BioRender : a , Huaiqiang, J. https://biorender.com/wbcolts (2026).

Article Snippet: For drug treatment, a CXCL12 neutralizing antibody (Merck Millipore) and an anti-mouse PD-1 antibody (Bio X Cell) were administered via intraperitoneal injection.

Techniques: Flow Cytometry

Journal: Non-coding RNA Research

Article Title: Aerobic exercise alleviates allergic airway inflammation by suppressing circMETTL9 -mediated formation of macrophage extracellular traps ⋆

doi: 10.1016/j.ncrna.2025.08.008

Figure Lengend Snippet: Communication Between Airway Epithelial Cells and Macrophages Mediated by CXCL12-CXCR4 Regulates METs. (A) The co-expression network of IGFBP3 and macrophage chemokines was predicted by the STRING database. (B) The binding of CXCL12 to the CXCR4 receptor was predicted in the CellphoneDB database. (C) Representative SYTOX Green staining in macrophages treated with or without CXCL12 (n = 3). (D) The protein expression of MPO and CitH3 in macrophages was detected by Western blot analysis (n = 3). (E – G) ELISA was used to detect the expression of CXCL12 in the cell supernatant of the co-culture system (n = 3). RT-qPCR was used to detect the expression of CXCL12 in BEAS-2B cells and CXCR4 in macrophages (n = 3). All data are expressed as means ± SD. ∗ P < 0.05. GAPDH was used as a loading control for all Western blot assays. All data are expressed as means ± SD. ∗ P < 0.05.

Article Snippet: To assess the expression levels of CXCL12, ELISA kits specifically for mouse CXCL12 (catalog #KE10049, Proteintech, Chicago, USA), and for human CXCL12 ELISA Kit (catalog #RK00266, ABclonal, China) were utilized according to the manufacturer's protocols.

Techniques: Expressing, Binding Assay, Staining, Western Blot, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Quantitative RT-PCR, Control

Journal: Non-coding RNA Research

Article Title: Aerobic exercise alleviates allergic airway inflammation by suppressing circMETTL9 -mediated formation of macrophage extracellular traps ⋆

doi: 10.1016/j.ncrna.2025.08.008

Figure Lengend Snippet: Overexpression of CircMETTL9 Counteracts the Reduction Effect of Aerobic Exercise on METs. (A) Schematic timeline of the experimental protocol. Day 0: AAV-LUNG-OE- circMETTL9 by the nasal drip. Days 14, 28, 42, and 56 represent intraperitoneal (i.p.) injections of OVA. Days 35–68 represent exposure to ovalbumin aerosol. Aerobic exercise adaptation occurred from days 35–37, and days 39 and 67 represent the initial and final physical tests. Aerobic exercise was initiated on day 42 and ended on day 66. Euthanasia was performed on day 70. (B) The expression of circMETTL9 was detected by RNA FISH staining (n = 6). (C) The expression of circMETTL9 was performed by RT-qPCR (n = 6). (D) ELISA was used to detect the expression of CXCL12 in the BALF (n = 6). (E) The mRNA expression of CXCL12 and CXCR4 was detected by RT-qPCR (n = 6). (F) Western blot analysis was used to detect the protein expression of CitH3 and MPO in the lung tissue (n = 6). (G) Representative immunofluorescence images of CitH3, MPO, and CD68 staining of lung tissues. (H) The result of the Western blot showed the effect of circMETTL9 overexpression on IGFBP3 and EIF4A3 expression (n = 6). A, OVA-induced asthmatic mice and infected with blank AAV; E, mice were subjected to aerobic exercise and infected with blank AAV; OE-A, OVA-induced asthmatic mice and infected with AAV overexpressing circMETTL9 ; OE-AE, OVA-induced asthmatic mice performed aerobic exercise and infected with AAV overexpressing circMETTL9 . All data were shown as the means ± SDs and were assessed by a paired two-tailed t -test. ∗ P < 0.05.

Article Snippet: To assess the expression levels of CXCL12, ELISA kits specifically for mouse CXCL12 (catalog #KE10049, Proteintech, Chicago, USA), and for human CXCL12 ELISA Kit (catalog #RK00266, ABclonal, China) were utilized according to the manufacturer's protocols.

Techniques: Over Expression, Aerosol, Expressing, Staining, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, Infection, Two Tailed Test

Journal: Molecular medicine reports

Article Title: Erythropoietin ameliorates renal interstitial fibrosis via the inhibition of fibrocyte accumulation.

doi: 10.3892/mmr.2015.3157

Figure Lengend Snippet: Figure 5. CXCL12, CCL21 and CXCL16 expression analysis. (A) Representative western blots for CXCL12, CCL21 and CXCL16. Semi‑quantitative analysis of protein expression levels of (B) CXCL12, (C) CCL21 and (D) CXCL16. Actin was used as an internal control. *P<0.05 vs. Sh; †P<0.05 vs. U+V and ‡P>0.05 vs. U+V. UUO, unilateral ureteral obstruction; Sh, control; U+V, UUO+vehicle; U+E1, UUO treated with 300 U/kg rhEPO; rhEPO, recombinant human erythropoietin; U+E2, UUO treated with 1,000 U/kg rhEPO; CXCL16, CXC chemokine ligand 16; CCL21, CC chemokine ligand 21.

Article Snippet: Proteins were electrophoretically transferred to nitrocellulose membranes (Millipore, Billerica, MA, USA) which were subsequently incubated with antibodies specific for α‐SMA (1:500), collagen I (1:1,000), fibronectin (1:400), CXCL12 (1:1,000) , CCL21 (1:600), CXCL16 (1:500) and β-actin (1:1,000), followed by incubation with secondary antibody conjugated with IRDye® infrared dye (Rockland Immunochemicals, Inc.).

Techniques: Expressing, Western Blot, Control, Recombinant

Journal: Molecular Medicine Reports

Article Title: CP-25 exerts anti-angiogenic effects on a rat model of adjuvant-induced arthritis by promoting GRK2-induced downregulation of CXCR4-ERK1/2 signaling in endothelial cells

doi: 10.3892/mmr.2019.10765

Figure Lengend Snippet: Expression of CXCL12 level in the synovium and the wall of blood vessel from rats. Representative immunohistochemical analyses of CXCL12 expression in the (A) synovium and (B) blood vessel walls, illustrating alterations in the joints of each group of rats (magnification, ×400). ODVs of CXCL12 in the (C) synovium and (D) vessel were markedly decreased in rats with AA following administration of CP-25 and MTX (>10 microscopic fields were observed in each section). (E) Levels of CXCL12 in the synovium assessed by ELISA. (F) Representative western blotting demonstrating CXCR4 expression in rat synovium. Western blotting data are expressed as the means ± standard deviation of three independent experiments. **P<0.01 vs. AA group (n=8–10 per group). (G and H) Correlation between pathological alterations in the pannus score and the expression of CXCL12 and CXCR4 in synovium. AA, adjuvant-induced arthritis; CP-25, paeoniflorin-6′-O-benzene sulfonate; CXCL12, C-X-C motif chemokine ligand 12; CXCR4, C-X-C chemokine receptor type 4; MTX, methotrexate; ODV, optical density value.

Article Snippet: Cell Counting kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. An ELISA kit for CXCL12 (cat. no. CSB-E08729r) was purchased from Cusabio, Inc. Antibodies against α-smooth muscle actin (α-SMA) (cat. no. ab32575), CXCR4 (cat. no. ab124824), GRK2 (cat. no. ab228705), ERK1/2 (cat. no. ab79853), phosphorylated (p)-ERK1/2 (cat. no. ab214362), CXCL12 (cat. no. ab9797), and β-actin (cat. no. ab115777) were purchased from Abcam.

Techniques: Expressing, Immunohistochemical staining, Enzyme-linked Immunosorbent Assay, Western Blot, Standard Deviation, Adjuvant

Journal: Molecular Medicine Reports

Article Title: CP-25 exerts anti-angiogenic effects on a rat model of adjuvant-induced arthritis by promoting GRK2-induced downregulation of CXCR4-ERK1/2 signaling in endothelial cells

doi: 10.3892/mmr.2019.10765

Figure Lengend Snippet: Effects of CP-25 on CXCL12-induced HUVEC proliferation, migration and tube formation. (A-C) Quantitative analysis of HUVEC proliferation, migration and tube formation induced by various concentrations of CXCL12 for 24 h. (D-F) Quantitative analysis of HUVEC proliferation, migration and tube formation following treatment with CXCL12 alone or in combination with CP-25 for 24 h. (G and H) Representative images of the Transwell and tube formation assays of HUVECs (magnification, ×200). Data are expressed as the means ± standard deviation of three independent experiments. # P<0.05, ## P<0.01 vs. control; *P<0.05, **P<0.01 vs. CXCL12. CP-25, paeoniflorin-6′-O-benzene sulfonate; CXCL12, C-X-C motif chemokine ligand 12; CXCR4, C-X-C chemokine receptor type 4.

Article Snippet: Cell Counting kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. An ELISA kit for CXCL12 (cat. no. CSB-E08729r) was purchased from Cusabio, Inc. Antibodies against α-smooth muscle actin (α-SMA) (cat. no. ab32575), CXCR4 (cat. no. ab124824), GRK2 (cat. no. ab228705), ERK1/2 (cat. no. ab79853), phosphorylated (p)-ERK1/2 (cat. no. ab214362), CXCL12 (cat. no. ab9797), and β-actin (cat. no. ab115777) were purchased from Abcam.

Techniques: Migration, Standard Deviation, Control

Journal: Molecular Medicine Reports

Article Title: CP-25 exerts anti-angiogenic effects on a rat model of adjuvant-induced arthritis by promoting GRK2-induced downregulation of CXCR4-ERK1/2 signaling in endothelial cells

doi: 10.3892/mmr.2019.10765

Figure Lengend Snippet: Effects of CP-25 on GRK2 and CXCR4 expression in HUVECs treated with CXCL12. Representative images of western blotting of (A) total, (D) cytoplasmic and (G) membrane expression of GRK2 and CXCR4. (B, C, E, F, H and I) Western blotting semi-quantification of GRK2 and CXCR4 expression. Data are expressed as the means ± standard deviation of three independent experiments. # P<0.05, ## P<0.01 vs. control; *P<0.05, **P<0.01 vs. CXCL12. CP-25, paeoniflorin-6′-O-benzene sulfonate; CXCL12, C-X-C motif chemokine ligand 12; CXCR4, C-X-C chemokine receptor type 4; GRK2; G protein-coupled receptor kinase 2.

Article Snippet: Cell Counting kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. An ELISA kit for CXCL12 (cat. no. CSB-E08729r) was purchased from Cusabio, Inc. Antibodies against α-smooth muscle actin (α-SMA) (cat. no. ab32575), CXCR4 (cat. no. ab124824), GRK2 (cat. no. ab228705), ERK1/2 (cat. no. ab79853), phosphorylated (p)-ERK1/2 (cat. no. ab214362), CXCL12 (cat. no. ab9797), and β-actin (cat. no. ab115777) were purchased from Abcam.

Techniques: Expressing, Western Blot, Membrane, Standard Deviation, Control

Journal: Molecular Medicine Reports

Article Title: CP-25 exerts anti-angiogenic effects on a rat model of adjuvant-induced arthritis by promoting GRK2-induced downregulation of CXCR4-ERK1/2 signaling in endothelial cells

doi: 10.3892/mmr.2019.10765

Figure Lengend Snippet: Effects of CP-25 on ERK1/2 expression in HUVECs treated with CXCL12. (A) Representative images of western blotting of the expression of ERK1/2 and p-ERK1/2. (B and C) Western blotting semi-quantification of ERK1/2 and p-ERK1/2. (D) Ratio of p-ERK/total-ERK. (E) Representative images of western blotting for the co-expression of GRK2 and CXCR4, and GRK2 and p-ERK1/2. (F and G) Western blotting semi-quantification of the binding between GRK2 and CXCR4, and GRK2 and p-ERK1/2. Data are expressed as the means ± standard deviation of three independent experiments. ## P<0.01 vs. control; *P<0.05, **P<0.01 vs. CXCL12. CP-25, paeoniflorin-6‰-O-benzene sulfonate; CXCL12, C-X-C motif chemokine ligand 12; CXCR4, C-X-C chemokine receptor type 4; GRK2; G protein-coupled receptor kinase 2; p, phosphorylated.

Article Snippet: Cell Counting kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. An ELISA kit for CXCL12 (cat. no. CSB-E08729r) was purchased from Cusabio, Inc. Antibodies against α-smooth muscle actin (α-SMA) (cat. no. ab32575), CXCR4 (cat. no. ab124824), GRK2 (cat. no. ab228705), ERK1/2 (cat. no. ab79853), phosphorylated (p)-ERK1/2 (cat. no. ab214362), CXCL12 (cat. no. ab9797), and β-actin (cat. no. ab115777) were purchased from Abcam.

Techniques: Expressing, Western Blot, Binding Assay, Standard Deviation, Control

Journal: Molecular Medicine Reports

Article Title: CP-25 exerts anti-angiogenic effects on a rat model of adjuvant-induced arthritis by promoting GRK2-induced downregulation of CXCR4-ERK1/2 signaling in endothelial cells

doi: 10.3892/mmr.2019.10765

Figure Lengend Snippet: Regulation of CXCR4 activity and signaling. (A) Upon ligand binding, CXCR4 could activate numerous signaling cascades, which may result in increased GRK2 membrane localization, weakening the inhibitory effect of GRK2 on ERK1/2 in the cytoplasm and enhancing ERK1/2 phosphorylation. (B) CP-25 could inhibit ERK1/2 phosphorylation by reducing the membrane localization of GRK2 and enhancing the inhibitory effect of GRK2 on ERK1/2 in the cytoplasm. CP-25, paeoniflorin-6′-O-benzene sulfonate; CXCL12, C-X-C motif chemokine ligand 12; CXCR4, C-X-C chemokine receptor type 4; GRK2; G protein-coupled receptor kinase 2; p, phosphorylated.

Article Snippet: Cell Counting kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. An ELISA kit for CXCL12 (cat. no. CSB-E08729r) was purchased from Cusabio, Inc. Antibodies against α-smooth muscle actin (α-SMA) (cat. no. ab32575), CXCR4 (cat. no. ab124824), GRK2 (cat. no. ab228705), ERK1/2 (cat. no. ab79853), phosphorylated (p)-ERK1/2 (cat. no. ab214362), CXCL12 (cat. no. ab9797), and β-actin (cat. no. ab115777) were purchased from Abcam.

Techniques: Activity Assay, Ligand Binding Assay, Membrane, Phospho-proteomics

Journal: BMC Cancer

Article Title: Cancer-associated fibroblasts induce sorafenib resistance of hepatocellular carcinoma cells through CXCL12/FOLR1

doi: 10.1186/s12885-023-11613-8

Figure Lengend Snippet: CAFs induce sorafenib resistance in HCC cells by secreting CXCL12. a The results of immunofluorescence showed that the expression of CXCL12 in CAFs in HCC tissues was significantly higher than that in paracancerous tissues (left). Statistical plot of fluorescence intensity of fibroblasts expressing α-SMA and CXCL12 in HCC tissues and paracancerous tissues (right). b ELISA showed that CAFs secreted higher level of CXCL12 than NFs. c , d Colony forming assays detected the sorafenib resistance of HCC cells (HepG2 and Huh7), after treated with the cellular supernatant of CAFs and NFs, sorafenib, and AMD3100. e , f Flow cytometry apoptosis assay detected the sorafenib resistance of HCC cells (HepG2 and Huh7), after treated with the cellular supernatant of CAFs and NFs, sorafenib, and AMD3100. g , h Western blotting was performed to detect the expression of β-actin, and Cleaved Caspase-3 in HCC cells (HepG2 and Huh7), which were treated with the cellular supernatant of CAFs, sorafenib, and AMD3100. The data presented mean ± SEM. * p < 0.01; ** p < 0.001; *** p < 0.0001; **** p < 0.00001

Article Snippet: Primary antibodies were used for IHC staining: CXCL12 (Boster, BA1389, 1:100), and α-SMA (Abcam, ab119952, 1:100).

Techniques: Immunofluorescence, Expressing, Fluorescence, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Apoptosis Assay, Western Blot

Journal: BMC Cancer

Article Title: Cancer-associated fibroblasts induce sorafenib resistance of hepatocellular carcinoma cells through CXCL12/FOLR1

doi: 10.1186/s12885-023-11613-8

Figure Lengend Snippet: CXCL12 induces sorafenib resistance in HCC cells by up-regulating the expression of FOLR1. a We found two datasets of cancer cells treated with CXCL12 protein (GSE15893 and GSE40017) in the GEO database. We took the intersection of the differentially expressed genes between these two datasets and combined them with the reported drug-resistant genes of HCC to obtain two genes. FOLR1 was the most significantly upregulated drug resistance-related gene upon CXCL12 treatment. b The qPCR was performed to detect the level of FOLR1 in Huh7 and HepG2, which treated with CXCL12 protein and AMD3100. c Western blotting was performed to detect the expression of β-actin and CXCR4 in CXCR4 knockdown HCC cells (Huh7 and HepG2). d , e Western blotting was performed to detect the expression of β-actin, CXCR4, FOLR1, and Cleaved Caspase-3 in Huh7 and HepG2, after treated with sorafenib, CXCL12 protein, and AMD3100. f , g Western blotting was performed to detect the expression of β-actin, CXCR4, FOLR1, and Cleaved Caspase-3 in Huh7 and HepG2, after treated with sorafenib, AMD3100, the supernatant of CAFs, and NFs. h , i Colony forming assay detected the sorafenib resistance of HCC cells (HepG2 and Huh7), after treated with sorafenib, CXCL12 protein, the supernatant of CAFs, and AMD3100. j , k Western blotting was performed to detect the expression of β-actin, FOLR1, CXCR4, and Cleaved Caspase-3 in Huh7 and HepG2, after treated with sorafenib, anti-CXCR4, CXCL12 protein, and the supernatant of CAFs. The data presented mean ± SEM. ** p < 0.001; *** p < 0.0001; **** p < 0.00001

Article Snippet: Primary antibodies were used for IHC staining: CXCL12 (Boster, BA1389, 1:100), and α-SMA (Abcam, ab119952, 1:100).

Techniques: Expressing, Western Blot, Knockdown

Journal: BMC Cancer

Article Title: Cancer-associated fibroblasts induce sorafenib resistance of hepatocellular carcinoma cells through CXCL12/FOLR1

doi: 10.1186/s12885-023-11613-8

Figure Lengend Snippet: CAFs enhance sorafenib resistance of HCC cells through CXCL12 in vivo. a Representative images of tumors in mice of CAFs + AMD3100 group, CAFs group, and NFs group after different treatments. b The tumor volume in different treatment groups. c The tumor proliferation trend in different treatment groups. d Pathological validation of tumors under a microscope (40X), after H&E staining and Immunohistochemistry in tumor tissues. The immunohistochemistry staining to detect the expression of Cleaved Caspase-3 in different treatment groups from the tumor tissues of mice. e The expression level of Cleaved Caspase-3 in different treatment groups from the tumors of mice. The data presented mean ± SEM. ** p < 0.001; *** p < 0.0001; **** p < 0.00001

Article Snippet: Primary antibodies were used for IHC staining: CXCL12 (Boster, BA1389, 1:100), and α-SMA (Abcam, ab119952, 1:100).

Techniques: In Vivo, Biomarker Discovery, Microscopy, Staining, Immunohistochemistry, Expressing