Journal: Science translational medicine

Article Title: The anti-CD40L monoclonal antibody AT-1501 promotes islet and kidney allograft survival and function in nonhuman primates

doi: 10.1126/scitranslmed.adf6376

Figure Lengend Snippet: (A) CD40L binding potency of AT-1501 (cyan) and Hu5C8 (orange) was determined by ELISA with recombinant human CD40L (rhuCD40L). Abatacept (purple), a variant of CTLA4-Ig that interferes with the CD28-CD80/86 costimulatory pathway, was used as a negative control. A450, absorbance at 450nm. (B) AT-1501 and Hu5C8 binding to human FcγRI, FcγRIIa, FcγRIIIa, and FcγRIIIb was measured by ELISA. (C to F) PAC-1 expression was measured in platelets from healthy controls incubated with soluble CD40L alone (C), Hu5C8 and sCD40L (D), Hu5C8 F(Ab’)2 lacking an Fc region and sCD40L (E), or AT-1501 and sCD40L (F). Blue tracings represent expression of PAC-1 following incubation with 5μg/mL sCD40L alone and red tracings represent expression of PAC-1 following incubation with the indicated combinations. (G) Platelet aggregation was measured in vitro using platelets incubated with the indicated combinations.

Article Snippet: CD40L binding assays ELISAs were run with Hu5C8 (ProSci, XP-5263BT), an anti-CD40L antibody, as a positive control and Abatacept (Bristol Myers Squibb), a variant of CTLA4-Ig antibody that does not bind CD40L, as a negative control.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Recombinant, Variant Assay, Negative Control, Expressing, Incubation, In Vitro

Journal:

Article Title: CD40-CD40 ligand (CD154) engagement is required but may not be sufficient for human T helper 1 cell induction of interleukin-2- or interleukin-15-driven, contact-dependent, interleukin-1? production by monocytes

doi: 10.1046/j.1365-2567.2000.00948.x

Figure Lengend Snippet: Effect of various mAb on IL-1β production in Th1/monocyte co-cultures. (a) IL-1β production in Th/monocyte co-cultures. (b) IL-1Ra production in Th1/monocyte or Th2/monocyte co-cultures. Freshly isolated monocytes were cultured with Th1 cells as described in Figure 5. The mAb or sCD40-Fc were added at the beginning of the culture. All the mAb were IgG1 and used at 10 µg/ml. The sCD40 was used at 1 µg/ml. The number of individual clones tested is given in parentheses. IL-1β production in the presence of irrelevant IgG1 was 153·5 ± 35·4 in different experiments. Bars indicate SEM.

Article Snippet: Recombinant human IL-2 and interferon-γ (IFN-γ) were from Biogen (Cambridge, MA); IL-15 and sCD40-Fc from Immunex (Seattle, WA).

Techniques: Isolation, Cell Culture, Clone Assay

Journal: Clinical Science (London, England : 1979)

Article Title: B-type natriuretic peptide is an independent predictor of endothelial function in man

doi: 10.1042/CS20110168

Figure Lengend Snippet: Plasma biomarker levels and FBF of the study subjects Values are medians (interquartile range)

Article Snippet: Blood (75 ml) was drawn into blood bottles containing citrate [tPA (tissue plasminogen activator)], heparin [E-selectin, ICAM-1 (intercellular adhesion molecule-1) and MPO (myeloperoxidase)], EDTA [sCD40 (soluble CD40), BNP and hs-CRP (high-sensitive C-reactive protein)], CTAD (citrate/theophylline/adenosine/dipyridamole) tubes (BectonDickinson) for PAI-1 (plasminogen-activator inhibitor-1) assay.

Techniques: Clinical Proteomics, Biomarker Discovery