Journal: Journal of Inflammation Research

Article Title: Increased CD163+ Macrophage Activation and High Expression of CD163 Promote Granulosa Cell Apoptosis in Polycystic Ovary Syndrome

doi: 10.2147/jir.s532920

Figure Lengend Snippet: Figure 3 Increased macrophages and elevated CD163 expression in macrophages in the ovary and uterus of PCOS mice. (a) Representative immunofluorescence images of CD163 and F4/80 in ovaries and uteri of control and PCOS mice (scale bar = 100 μm). (b) Quantification of CD163+, F4/80+, and F4/80+CD163+ cell proportions in the ovaries and uteri of control and PCOS mice. *p < 0.05, **p < 0.01, ns: p > 0.05.

Article Snippet: CD163 protein was acquired from MedChemExpress (Monmouth Junction,

Techniques: Expressing, Immunofluorescence, Control

Journal: Journal of Inflammation Research

Article Title: Increased CD163+ Macrophage Activation and High Expression of CD163 Promote Granulosa Cell Apoptosis in Polycystic Ovary Syndrome

doi: 10.2147/jir.s532920

Figure Lengend Snippet: Figure 4 Increased M1 and M2 macrophages and elevated CD163 expression in M1 and M2 macrophages in the ovary and uterus of PCOS mice. (a) Representative immunofluorescence images of CD163, F4/80, iNOS, and Arg1 in the ovaries of control and PCOS mice (scale bar = 50 μm). (b) Representative immunofluorescence images of CD163, F4/80, iNOS, and Arg1 in the uterus of control and PCOS mice (scale bar = 50 μm). (c) Quantification of F4/80+iNOS+CD163+ and F4/80+Arg1+CD163+ cell proportions in the ovaries. (d) Quantification of F4/80+iNOS+CD163+ and F4/80+Arg1+CD163+ cell proportions in the uterus. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: CD163 protein was acquired from MedChemExpress (Monmouth Junction,

Techniques: Expressing, Immunofluorescence, Control

Journal: Journal of Inflammation Research

Article Title: Increased CD163+ Macrophage Activation and High Expression of CD163 Promote Granulosa Cell Apoptosis in Polycystic Ovary Syndrome

doi: 10.2147/jir.s532920

Figure Lengend Snippet: Figure 8 Increased apoptosis in CD163+ ovarian cells from PCOS mice. (a) Representative images of TUNEL and CD163 in the ovaries of control and PCOS mice via immunofluorescence analysis (scale bar = 50 μm). (b) Quantification of TUNEL+ and CD163+TUNEL+ cell proportions in the ovaries. **p < 0.01, ***p < 0.001.

Article Snippet: CD163 protein was acquired from MedChemExpress (Monmouth Junction,

Techniques: TUNEL Assay, Control, Immunofluorescence

Journal: Frontiers in Genetics

Article Title: SYK Is Associated With Malignant Phenotype and Immune Checkpoints in Diffuse Glioma

doi: 10.3389/fgene.2022.899883

Figure Lengend Snippet: Expression pattern of SYK by single-cell sequencing analysis and the association between SYK expression and M2 macrophage infiltration. (A,B) SYK expression in the different cell types; (B–F) The correlation between SYK expression and CD163 and VSIG4 expression in the four cohorts. (G,H) The correlation between SYK expression and CD163 expression by IHC staining.

Article Snippet: Immunohistochemistry (IHC) was performed using a primary antibody against PD-L1 (Cell Signaling Technology Pathways, United States), CD163 (Proteintech, China), and SYK (Proteintech, China).

Techniques: Expressing, Sequencing, Immunohistochemistry

Journal: Experimental and Therapeutic Medicine

Article Title: Role of SARS‑CoV‑2 nucleocapsid protein in affecting immune cells and insights on its molecular mechanisms

doi: 10.3892/etm.2023.12203

Figure Lengend Snippet: Quantification of TNF-α, IL-10, IL-6 and CD163. The concentrations of (A) TNF-α, (B) IL-10, (C) IL-6 and (D) CD163 were determined in the supernatant of cultured Raw264.7 cells treated with the severe acute respiratory syndrome coronavirus 2 N protein. Data are presented as mean ± SD (n=3). * P<0.05 and ** P<0.01 vs. blank control.

Article Snippet: PageRuler TM Prestained protein ladder (cat. no. 26616) was purchased from Thermo Fisher Scientific, Inc. Trypsin-EDTA (0.25%) used to digest Raw264.7 cells for cell passage was purchased from Gibco; Thermo Fisher Scientific Inc. TNF-α Elisa kit (Cat. no. EK0527), IL-6 Elisa Kit (Cat. no. EK0411), IL-10 Elisa Kit (Cat. no. EK0417) and CD163 ELISA kit (Cat. no. EK1146) were purchased from Boster Biological Technology co., Ltd.).

Techniques: Cell Culture, Control

Journal: International Journal of Molecular Sciences

Article Title: Macrophage Activation Markers Predict Liver-Related Complications in Primary Biliary Cholangitis

doi: 10.3390/ijms23179814

Figure Lengend Snippet: Clinical characteristics of patients with primary biliary cholangitis.

Article Snippet: For the non-invasive evaluation of IP, the serum levels of sCD163 were determined using a kit (Cusabio Technology, Houston, TX, USA) [ ], while sMR was measured using an enzyme-linked immunosorbent assay (ELISA) kit (LifeSpan BioSciences, Seattle, WA, USA) [ ].

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Macrophage Activation Markers Predict Liver-Related Complications in Primary Biliary Cholangitis

doi: 10.3390/ijms23179814

Figure Lengend Snippet: Diagnostic accuracy of intestinal permeability markers for symptom development in patients with primary biliary cholangitis.

Article Snippet: For the non-invasive evaluation of IP, the serum levels of sCD163 were determined using a kit (Cusabio Technology, Houston, TX, USA) [ ], while sMR was measured using an enzyme-linked immunosorbent assay (ELISA) kit (LifeSpan BioSciences, Seattle, WA, USA) [ ].

Techniques: Diagnostic Assay, Permeability

Journal: International Journal of Molecular Sciences

Article Title: Macrophage Activation Markers Predict Liver-Related Complications in Primary Biliary Cholangitis

doi: 10.3390/ijms23179814

Figure Lengend Snippet: Receiver operating characteristic curves for soluble mannose receptor and soluble CD163 alone and combined.

Article Snippet: For the non-invasive evaluation of IP, the serum levels of sCD163 were determined using a kit (Cusabio Technology, Houston, TX, USA) [ ], while sMR was measured using an enzyme-linked immunosorbent assay (ELISA) kit (LifeSpan BioSciences, Seattle, WA, USA) [ ].

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Macrophage Activation Markers Predict Liver-Related Complications in Primary Biliary Cholangitis

doi: 10.3390/ijms23179814

Figure Lengend Snippet: Univariate and multivariate analyses of predictive markers for symptom development in patients with primary biliary cholangitis.

Article Snippet: For the non-invasive evaluation of IP, the serum levels of sCD163 were determined using a kit (Cusabio Technology, Houston, TX, USA) [ ], while sMR was measured using an enzyme-linked immunosorbent assay (ELISA) kit (LifeSpan BioSciences, Seattle, WA, USA) [ ].

Techniques: Biomarker Discovery

Journal: International Journal of Molecular Sciences

Article Title: Macrophage Activation Markers Predict Liver-Related Complications in Primary Biliary Cholangitis

doi: 10.3390/ijms23179814

Figure Lengend Snippet: The cumulative incidence of PBC-related complications according to CD163. Patients with PBC having sCD163 of ≥30.1 tended to have more liver complications more frequently than those with sCD163 of <30.1 ( p = 0.0502). PBC: primary biliary cholangitis.

Article Snippet: For the non-invasive evaluation of IP, the serum levels of sCD163 were determined using a kit (Cusabio Technology, Houston, TX, USA) [ ], while sMR was measured using an enzyme-linked immunosorbent assay (ELISA) kit (LifeSpan BioSciences, Seattle, WA, USA) [ ].

Techniques:

Journal: Scientific Reports

Article Title: Circulating HIV DNA Correlates With Neurocognitive Impairment in Older HIV-infected Adults on Suppressive ART

doi: 10.1038/srep17094

Figure Lengend Snippet: HIV DNA and cytokines levels between age group.

Article Snippet: Levels of selected monocyte activation and inflammatory markers were measured in CSF and blood plasma by enzyme-linked immunosorbent assay (ELISA): sCD163 (Trillium Diagnostics, Brewer, ME, USA); or by electrochemiluminescence multiplex assay: MCP-1, IL-8, IL-6 and TNF-α, (Meso Scale Diagnostics, Rockville, MD, USA), according to the manufacturer’s procedures.

Techniques: Clinical Proteomics

Journal: PLoS ONE

Article Title: Soluble CD163 as a Marker of Macrophage Activity in Newly Diagnosed Patients with Multiple Sclerosis

doi: 10.1371/journal.pone.0098588

Figure Lengend Snippet: Four graphs (2a–2d) with levels of: sCD163 in CSF (2a); serum (2b); the sCD163 CSF/serum ratios (2c); and the sCD163 indexes (2d). The box plots displays the distribution of the parameters for each group, RRMS, PPMS; SPMS; CIS and SC. The line in the box indicating the median level, whiskers fences the upper and lower quartiles, and “o” labels the outliers. Abbreviations: RRMS (relapsing-remitting MS), PPMS (primary-progressive MS), CIS (clinically isolated syndrome), SC (symptomatic controls), CSF (cerebrospinal fluid), CI (confidence interval).

Article Snippet: In a pilot experiment, results were compared with a commercial sCD163 assay (Macro163TM, IQ-products Groningen NL), showing good correlation, however with a bias due to lack of assay standardisation as described earlier for serum samples .

Techniques: Isolation

Journal: PLoS ONE

Article Title: Soluble CD163 as a Marker of Macrophage Activity in Newly Diagnosed Patients with Multiple Sclerosis

doi: 10.1371/journal.pone.0098588

Figure Lengend Snippet: Median levels of sCD163 in serum and CSF samples as determined by ELISA.

Article Snippet: In a pilot experiment, results were compared with a commercial sCD163 assay (Macro163TM, IQ-products Groningen NL), showing good correlation, however with a bias due to lack of assay standardisation as described earlier for serum samples .

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: Soluble CD163 as a Marker of Macrophage Activity in Newly Diagnosed Patients with Multiple Sclerosis

doi: 10.1371/journal.pone.0098588

Figure Lengend Snippet: Results from linear regression analyses of differences between patients with MS/CIS and SC.

Article Snippet: In a pilot experiment, results were compared with a commercial sCD163 assay (Macro163TM, IQ-products Groningen NL), showing good correlation, however with a bias due to lack of assay standardisation as described earlier for serum samples .

Techniques:

Journal: PLoS ONE

Article Title: Vitamin D deficiency is associated with IL-6 levels and monocyte activation in HIV-infected persons

doi: 10.1371/journal.pone.0175517

Figure Lengend Snippet: Geometric means (with 95% CI) of plasma biomarkers, monocytes and T-cell phenotypes, by vitamin D levels and percent difference of deficient to those not deficient, at study enrollment, the Study to Understand the Natural History of HIV/AIDS in the Era of Effective Therapy, United States, 2004–2006

Article Snippet: The following biomarkers were measured at the National Institutes of Allergy and Infectious Diseases: IL-6 (Meso Scale Discovery, Rockville, MD) using electrochemiluminescence immunoassays and sCD163 (Aviscera Bioscience, Inc., Santa Clara, CA) using an ELISA based assay.

Techniques: Clinical Proteomics

Journal: PLoS ONE

Article Title: Vitamin D deficiency is associated with IL-6 levels and monocyte activation in HIV-infected persons

doi: 10.1371/journal.pone.0175517

Figure Lengend Snippet: Association of biomarkers and immune phenotypes with vitamin D deficiency, the Study to Understand the Natural History of HIV/AIDS in the Era of Effective Therapy, United States, 2004–2006

Article Snippet: The following biomarkers were measured at the National Institutes of Allergy and Infectious Diseases: IL-6 (Meso Scale Discovery, Rockville, MD) using electrochemiluminescence immunoassays and sCD163 (Aviscera Bioscience, Inc., Santa Clara, CA) using an ELISA based assay.

Techniques: Clinical Proteomics