Journal: Journal of Antimicrobial Chemotherapy

Article Title: No accelerated progression of subclinical atherosclerosis with integrase strand transfer inhibitors compared to non-nucleoside reverse transcriptase inhibitors

doi: 10.1093/jac/dkae383

Figure Lengend Snippet: Characteristics of patients according to the antiretroviral regimen composition

Article Snippet: D-dimer [Human D2D (D-Dimer) ELISA Kit], soluble ICAM-1 (sICAM-1) [Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit], soluble CD14 (sCD14) [Human sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit] and soluble CD163 (sCD163) [Human sCD163 (Soluble Cluster of Differentiation 163) ELISA Kit] were measured by enzyme-linked immunosorbent assay (ELISA Kits, Elabscience Biotechnology Inc., USA) with an automated instrument (Dynex DS2 ® ELISA system).

Techniques: Cell Counting

Journal: Journal of Antimicrobial Chemotherapy

Article Title: No accelerated progression of subclinical atherosclerosis with integrase strand transfer inhibitors compared to non-nucleoside reverse transcriptase inhibitors

doi: 10.1093/jac/dkae383

Figure Lengend Snippet: Characteristics of patients according to the progression of cIMT during the 2 year follow-up

Article Snippet: D-dimer [Human D2D (D-Dimer) ELISA Kit], soluble ICAM-1 (sICAM-1) [Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit], soluble CD14 (sCD14) [Human sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit] and soluble CD163 (sCD163) [Human sCD163 (Soluble Cluster of Differentiation 163) ELISA Kit] were measured by enzyme-linked immunosorbent assay (ELISA Kits, Elabscience Biotechnology Inc., USA) with an automated instrument (Dynex DS2 ® ELISA system).

Techniques: Cell Counting

Journal: Journal of Antimicrobial Chemotherapy

Article Title: No accelerated progression of subclinical atherosclerosis with integrase strand transfer inhibitors compared to non-nucleoside reverse transcriptase inhibitors

doi: 10.1093/jac/dkae383

Figure Lengend Snippet: Univariate and multivariate analysis of factors associated with cIMT increase analysed as a continuous variable during the 2 year follow-up

Article Snippet: D-dimer [Human D2D (D-Dimer) ELISA Kit], soluble ICAM-1 (sICAM-1) [Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit], soluble CD14 (sCD14) [Human sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit] and soluble CD163 (sCD163) [Human sCD163 (Soluble Cluster of Differentiation 163) ELISA Kit] were measured by enzyme-linked immunosorbent assay (ELISA Kits, Elabscience Biotechnology Inc., USA) with an automated instrument (Dynex DS2 ® ELISA system).

Techniques: Cell Counting

Journal: Journal of Antimicrobial Chemotherapy

Article Title: No accelerated progression of subclinical atherosclerosis with integrase strand transfer inhibitors compared to non-nucleoside reverse transcriptase inhibitors

doi: 10.1093/jac/dkae383

Figure Lengend Snippet: Patients’ characteristics according to the antiretroviral regimen composition after propensity score matching

Article Snippet: D-dimer [Human D2D (D-Dimer) ELISA Kit], soluble ICAM-1 (sICAM-1) [Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit], soluble CD14 (sCD14) [Human sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit] and soluble CD163 (sCD163) [Human sCD163 (Soluble Cluster of Differentiation 163) ELISA Kit] were measured by enzyme-linked immunosorbent assay (ELISA Kits, Elabscience Biotechnology Inc., USA) with an automated instrument (Dynex DS2 ® ELISA system).

Techniques: Cell Counting

Journal: Translational Neuroscience

Article Title: Activating α7nAChR suppresses systemic inflammation by mitigating neuroinflammation of the medullary visceral zone in sepsis in a rat model

doi: 10.1515/tnsci-2022-0345

Figure Lengend Snippet: Concentration of serum cytokines among different groups of rats. TNF-α: tumor necrosis factor alpha; IL-6: interleukin 6; IL-10: interleukin 10; sCD14: soluble CD14, precepsin; HMGB-1: high mobility group box-1. (a–c) Serum concentration (pg/mL) of TNF-α, IL-6, and IL-10 among different groups; (d) serum sCD14 concentration (ng/mL) among different groups, usually as an inflammatory marker in early stage of sepsis; (e) serum concentration of HMGB1 (pg/mL), usually as an inflammatory marker in late stage of sepsis; sepsis causes serum level of inflammatory cytokines, including TNF-α, IL-6, IL-10, sCD14, and HMGB1 significantly increased; these cytokines levels in the model group, the GTS-21 group, and the MLA group were significantly higher than those in the control group and the sham group, except IL-10 and sCD14, GTS-21 significantly decreased the other cytokines’ level; all inflammatory cytokines levels reached the highest in the MLA group, and there were significant differences when compared to the GST-21 groups. * P < 0.05; ** P < 0.01.

Article Snippet: 100 μL of serum were taken to the wells of these kits such as TNF-α (batch number: E-EL-R0019c; Elabscience), IL-6 (batch number: E-EL-R0015c; Elabscience), IL-10 (lot number: E-EL-R0016c; Elabscience), HMGB1 (batch number: E-EL-R0505c; Elabscience), and sCD14 (batch number: CSB-E11178r; Cusabio), respectively, According to the manufacturer’s instructions, after such steps as incubation, washing, reaction with working solution and densitometry, serum contents of TNF-α, IL-6, IL-10, HMGB1, and sCD14 were determined with ELISA.

Techniques: Concentration Assay, Marker, Control

Journal: GMS infectious diseases

Article Title: Correlation of (1→3)-β-D-glucan with other inflammation markers in chronically HIV infected persons on suppressive antiretroviral therapy

doi: 10.3205/id000018

Figure Lengend Snippet: Results for all investigated biomarkers [median and (IQR) or mean ± standard deviation (SD) are displayed] and correlation of β-D-glucan (BDG; squareroot transformed to achieve distribution close to normal) with other biomarkers

Article Snippet: Plasma samples were stored at −80°C at the day of collection and 41 samples from 41 subjects with suppressed levels of HIV RNA were randomly selected for retrospective evaluation of BDG levels and other biomarkers. sCD14 (Trillium Diagnostics, Brewer, ME, USA) and neopterin (Thermo Scientific, Waltham, MA, USA) were measured by enzyme-linked immunosorbent assays (ELISAs), while IL-8, IL-6 and TNF-α were measured by electrochemiluminescence multiplex assay (Meso Scale Diagnostics, Rockville, MD, USA), all according to the manufacturer’s procedures.

Techniques: Standard Deviation, Transformation Assay

Journal:

Article Title: Acute-Phase Concentrations of Lipopolysaccharide (LPS)-Binding Protein Inhibit Innate Immune Cell Activation by Different LPS Chemotypes via Different Mechanisms

doi: 10.1128/IAI.73.1.193-200.2005

Figure Lengend Snippet: hd-LBP inhibits binding of R and S LPS chemotypes to sCD14. Binding of R-type LPS from E. coli F515 (Re-LPS) (A), R-type LPS from S. enterica serovar Typhimurium TV 119 (Ra-LPS) (B), and S-type LPS from E. coli O111:B4 (C) to recombinant human sCD14 and the modulating effects of increasing concentrations of LBP were analyzed by native PhastGel electrophoresis. Following incubation at 37°C for 10 min, samples were subjected to automated native electrophoresis on 20% PhastGels and silver staining by using the PhastSystem apparatus. The positions of uncomplexed sCD14, sCD14-LPS complexes, LBP, and the LPS preparations are indicated. Arrows indicate different sCD14-LPS complexes containing most likely LPS of different chain lengths (C). As a loading control, the native samples were subsequently adjusted to a final concentration of 2% (wt/vol) SDS and additionally analyzed by PhastGel SDS electrophoresis and silver staining (lower panels).

Article Snippet: Briefly, R-type LPS from E. coli F515 or S-type LPS from E. coli O111:B4 at a concentration of 0.025 or 0.113 μg/μl, respectively, was incubated in Dulbecco's phosphate-buffered saline (without magnesium or calcium; Life Technologies/GIBCO BRL, Eggenstein, Germany) in the absence or presence of 0.25 μg of recombinant human sCD14 (Biometec, Greifswald, Germany)/μl at 37°C for 10 min. For analysis of LBP effects, recombinant human LBP (Xoma Corp.) was added at final concentrations of 0.0005, 0.0025, 0.025, 0.125, and 0.25 μg/μl.

Techniques: Binding Assay, Recombinant, Electrophoresis, Incubation, Silver Staining, Control, Concentration Assay

Journal: Microorganisms

Article Title: Markers of Microbial Translocation and Immune Activation Predict Cognitive Processing Speed in Heavy-Drinking Men Living with HIV

doi: 10.3390/microorganisms5040064

Figure Lengend Snippet: Results of individual GEE models predicting processing speed (WAIS Digit Symbol test) using immune biomarkers, average weekly drinks, and education.

Article Snippet: Enzyme-linked immunosorbent assays (ELISA) to quantify LPS (MyBiosource, San Diego, CA, USA), sCD14 (Enzo Life Sciences, Farmingdale, NY, USA), and EndoCAb (Hycult, Plymouth Meeting, PA, USA) were performed according to manufacturer instructions.

Techniques:

Journal: Microorganisms

Article Title: Markers of Microbial Translocation and Immune Activation Predict Cognitive Processing Speed in Heavy-Drinking Men Living with HIV

doi: 10.3390/microorganisms5040064

Figure Lengend Snippet: Results of GEE model predicting processing speed (WAIS Digit Symbol test) using all immune biomarkers, average weekly drinks, and education

Article Snippet: Enzyme-linked immunosorbent assays (ELISA) to quantify LPS (MyBiosource, San Diego, CA, USA), sCD14 (Enzo Life Sciences, Farmingdale, NY, USA), and EndoCAb (Hycult, Plymouth Meeting, PA, USA) were performed according to manufacturer instructions.

Techniques: