Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Glavonoid-rich oil supplementation reduces stearoyl-coenzyme A desaturase 1 expression and improves systemic metabolism in diabetic, obese KK-A y mice.

doi: 10.1016/j.biopha.2021.111714

Figure Lengend Snippet: Fig. 9. GRO supplementation reduced the protein levels of SCD1 and PPARα and increased the level of phosphorylated AMPK in the liver. (A) Western blot analysis of SCD1 in the liver of 20-week-old mice. (B) Western blot analysis of PPARα in the liver of 20-week-old mice. (C) The ratio of phosphorylated AMPK to total AMPK levels in the liver of 20-week-old mice, as determined by Western blot analysis. Cont: control mice; GRO8: 0.8% GRO mice. The data were analyzed with Student’s t- test. *p < 0.05, *p < 0.01. Values are means ± SD (n = 5).

Article Snippet: After electrophoresis, the separated proteins were transferred to a polyvinylidene difluoride membrane (Immobilon, 0.2 μm pore, (Merck Millipore Burlington, MA, USA)) and incubated overnight at 4 ◦C with the following primary antibodies: polyclonal rabbit anti-mouse PPARα (1:3000, GeneTex, Los Angeles, CA, USA), β-actin (1:3000, GeneTex), total AMPKα1 (1:3000, GeneTex), phosphorylated AMPKα1 (Thr172) (1:1000, Cell Signaling Technology, Danvers, MA, USA), and SCD1 (1:1000, R&D Systems Minneapolis, MN, USA).

Techniques: Western Blot, Control

Journal: Endocrinology

Article Title: Effect of Leptin Replacement on PCSK9 in ob/ob Mice and Female Lipodystrophic Patients

doi: 10.1210/en.2015-1624

Figure Lengend Snippet: Antibody Table

Article Snippet: SCD1 , Unknown , SCD1 antibody , Santa Cruz Biotechnology, Inc, sc-14720 , Goat; polyclonal , 1:1000.

Techniques: Sequencing

Journal: Cell Death and Differentiation

Article Title: SIRT3 promotes lipophagy and chaperon-mediated autophagy to protect hepatocytes against lipotoxicity

doi: 10.1038/s41418-019-0356-z

Figure Lengend Snippet: SIRT3 suppresses lipogenesis in lipotoxic hepatocytes. a SCD1 protein level in vector and SIRT3OE hepatocytes treated with or without P/O mixture for 24 h. b SIRT3 and SCD1 protein levels in SIRT3 and SCD1 double overexpressed hepatocytes. The lipid content c and the cellular TG content d in SIRT3 and SCD1 double overexpressed hepatocytes. White: blank, vector; gray: blank, SIRT3OE; light gray: P/O, vector; black: P/O, SIRT3OE. Data are shown as mean ± S.D., n = 6, * p < 0.05, and *** p < 0.001, vector vs. SIRT3OE, ### p < 0.001 blank vs. P/O treatment. && p < 0.01, &&& p < 0.001, SCD1OE vs. vector. One-way ANOVA was used to calculate the p -values

Article Snippet: The plasmid of pCMV3-SCD1-N-Myc was purchased from Sino Biological (MG51311-NM, Wayne, PA, USA).

Techniques: Plasmid Preparation

Journal: Cell Death and Differentiation

Article Title: SIRT3 promotes lipophagy and chaperon-mediated autophagy to protect hepatocytes against lipotoxicity

doi: 10.1038/s41418-019-0356-z

Figure Lengend Snippet: Schematic of the role of SIRT3 in protecting hepatocytes against lipotoxicity. SIRT3 activates AMPK to enhance macroautophagy and CMA on LDs and promote LDs dispersion on detyrosinated MTs, inhibits SCD1 expression to suppress lipogenesis, and deacetylates LCAD to increase fatty acid oxidation in mitochondria, resulting in attenuation of lipotoxicity in hepatocytes

Article Snippet: The plasmid of pCMV3-SCD1-N-Myc was purchased from Sino Biological (MG51311-NM, Wayne, PA, USA).

Techniques: Expressing