Journal: International Journal of Biological Sciences

Article Title: CXCL8 Drives MMP1 Upregulation and Promotes Metastatic Progression in Oral Cancer Through CXCR1/2-Mediated JAK1/STAT3 Activation

doi: 10.7150/ijbs.115990

Figure Lengend Snippet: CXCL8 is overexpressed in OSCC and associated with poor prognosis. (A) Venn diagram of upregulated DEGs identified from three GEO datasets: GSE13601 (n=29 OSCC, 29 normal), GSE143905 (n=5 OSCC, 5 normal), and GSE31056 (n=23 OSCC, 24 normal). (B) Hub genes ranked by interaction degree within the protein-protein interaction network (Cytoscape). (C) Heatmap showing expression levels of the top 40 upregulated DEGs in OSCC tissues from GSE74530 (n=6 OSCC, 6 normal). (D) Volcano plot of significantly altered genes in GSE74530 ; red: upregulated, blue: downregulated. (E) Differential expression of CXCL8 between OSCC and normal tissues in GSE74530 . (F) Kaplan-Meier analysis showing overall survival in HNSCC patients with high and low CXCL8 expression (UCSC Xena).

Article Snippet: The human OSCC cell lines SCC9, SCC4 (from ATCC) and HSC3 (from Sigma-Aldrich) were used in this study.

Techniques: Expressing, Quantitative Proteomics

Journal: International Journal of Biological Sciences

Article Title: CXCL8 Drives MMP1 Upregulation and Promotes Metastatic Progression in Oral Cancer Through CXCR1/2-Mediated JAK1/STAT3 Activation

doi: 10.7150/ijbs.115990

Figure Lengend Snippet: CXCL8 expression positively correlates with OSCC cell motility. (A)-(B) Wound healing and Transwell migration assays comparing SCC9, SCC4, and HSC3 cell lines (n=4). (C) Western blot analysis of CXCL8 protein levels in the three cell lines (n=4). (D)-(G) The migration and wound closure were evaluated following CXCL8 (25-50 ng/ml) treatment in SCC4 and HSC3 cells (n=4). (H)-(I) Migration and wound healing ability of SCC4 subpopulations vs. parental SCC4 cells were assessed (n=4). (J)-(K) CXCL8 mRNA and protein expression in highly migratory SCC4 cells compared to parental cells were assessed (n=4). Results are expressed as the mean ± SD of four independent experiments. * p < 0.05 compared with the control group.

Article Snippet: The human OSCC cell lines SCC9, SCC4 (from ATCC) and HSC3 (from Sigma-Aldrich) were used in this study.

Techniques: Expressing, Migration, Western Blot, Control

Journal: International Journal of Biological Sciences

Article Title: CXCL8 Drives MMP1 Upregulation and Promotes Metastatic Progression in Oral Cancer Through CXCR1/2-Mediated JAK1/STAT3 Activation

doi: 10.7150/ijbs.115990

Figure Lengend Snippet: CXCL8 promotes OSCC cell migration by inducing MMP1 expression. (A) TIMER2.0 analysis of correlations between CXCL8 and MMPs in HNSC (n=522). (B)-(D) CXCL8-induced MMP1 expression was examined by qPCR and Western blot analysis in SCC4 and HSC3 cells (n=4). (E) Validation of MMP1 knockdown efficiency by Western blot (n=4). (F)-(I) Functional analysis confirmed the effect of MMP1 knockdown on CXCL8-induced cell migration and wound healing (n=4). (J)-(K) MMP1 mRNA and protein expression of highly migratory SCC4 subpopulations were analyzed by Western blotting and qPCR (n=4). Results are expressed as the mean ± SD of four independent experiments. * p < 0.05 compared with the control group; # p < 0.05 compared with the CXCL8-treated group.

Article Snippet: The human OSCC cell lines SCC9, SCC4 (from ATCC) and HSC3 (from Sigma-Aldrich) were used in this study.

Techniques: Migration, Expressing, Western Blot, Biomarker Discovery, Knockdown, Functional Assay, Control

Journal: Aging (Albany NY)

Article Title: Combined impacts of histamine receptor H1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma

doi: 10.18632/aging.204089

Figure Lengend Snippet: Clinical relevance of histamine receptor H1 (HRH1) levels in head and neck squamous cell carcinoma (HNSCC) or oral squamous cell carcinoma (OSCC) patients obtained from TCGA and GEO databases. ( A , B ) HRH1 expression was higher in HNSCC tissues ( A ) and OSCC tissues ( B ) than in normal tissues (database sources: TCGA and GSE78060). ( C ) HRH1 expression levels in OSCC from GSE78060 were compared according to the tumor size (T stages). ( D ) Kaplan-Meier curves for overall and disease-specific survival (left panel) and recurrence-free survival (right panel) of patients with HNSCC and OSCC, as categorized according to high or low expression of HRH1 . The p value indicates a comparison between patients with HRH1 high and HRH1 low . (database sources: TCGA and GSE31056).

Article Snippet: Actually, our present study further examined the genotypes of rs901865 and rs346074 in five OSCC cell lines (SCC9, HSC-3, HSC-3M, SAS, and OECM1) and found that OECM1 cells carrying the rs901865 CT genotype and the rs346074 TT genotype expressed lower HRH1 protein levels compared to SAS, HSC-3, and HSC-3M cells carrying the CC genotype of both SNPs, suggesting that the T allele of rs901865 or rs346074 may produce a decrease in HRH1 levels in OSCC and subsequently cause smaller tumor sizes in OSCC patients carrying one minor allele of rs901865 or rs346074.

Techniques: Expressing, Comparison

Journal: Aging (Albany NY)

Article Title: Combined impacts of histamine receptor H1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma

doi: 10.18632/aging.204089

Figure Lengend Snippet: Correlations of histamine receptor H1 (HRH1) rs346074 and rs901865 genotypes with HRH1 protein levels in five oral squamous cell carcinoma (OSCC) cell lines. Upper panel, HRH1 rs346074 or rs901865 genotypes in OSCC cells (SAS, HSC-3M, HSC-3, SCC9, and OECM1) were detected by a TaqMan SNP Genotyping Assay. Lower panel, protein levels of HRH1 were detected by a Western blot analysis using two HRH1-specific antibodies.

Article Snippet: Actually, our present study further examined the genotypes of rs901865 and rs346074 in five OSCC cell lines (SCC9, HSC-3, HSC-3M, SAS, and OECM1) and found that OECM1 cells carrying the rs901865 CT genotype and the rs346074 TT genotype expressed lower HRH1 protein levels compared to SAS, HSC-3, and HSC-3M cells carrying the CC genotype of both SNPs, suggesting that the T allele of rs901865 or rs346074 may produce a decrease in HRH1 levels in OSCC and subsequently cause smaller tumor sizes in OSCC patients carrying one minor allele of rs901865 or rs346074.

Techniques: TaqMan SNP Genotyping Assay, Western Blot