Journal: bioRxiv

Article Title: The SON RNA splicing factor is required for intracellular trafficking that promotes centriole assembly

doi: 10.1101/2021.01.29.428802

Figure Lengend Snippet: A) Structured Illumination Microscopy (SIM) images of siRNA treated, S phase arrested cells induced for PLK4 expression and stained for centriole assembly factors. Scale bar = 1 μm. B) Fluorescence intensity quantifications of widefield microscopy images (Supplementary Figureure 2A). N = CETN: siControl, 851 centrosomes; siSON, 925 centrosomes; FLAG-PLK4: siControl, 111 centrosomes; siSON, 131 centrosomes; CEP152: siControl, 136 centrosomes; siSON, 143 centrosomes; CEP192: siControl, 124 centrosomes; siSON, 123 centrosomes; STIL: siControl, 136 centrosomes; siSON 138 centrosomes; SAS-6: siControl, 132 centrosomes; siSON, 148 centrosomes; CPAP: siControl, 120 centrosomes; siSON, 122 centrosomes; Cep135: siControl, 91 centrosomes; siSON, 119 centrosomes. Data compiled from two biological replicates. Bar and error bars: mean and standard deviation.

Article Snippet: Primary antibodies: 1:2000 α-ψ-tubulin (Sigma, DQ19); 1:200 α-FLAG (Sigma, M2); 1:2500 α-CEP152 (Bethyl A302-480A); 1:2000 α-CEP192 (the generous gift of A. Holland, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD); 1:2500 α-STIL (Bethyl SIL A302-441A); 1:2000 α-SAS-6 (Bethyl A301-802A); 1:500 α-CPAP (Proteintech CENPJ 11517-1-AP); 1:300 α-CEP135 (Proteintech 24428-1-AP); 1:4000 α-SON (Thermo Fisher Scientific PA5-65107); 1:2000 α-Pericentrin (Abcam ab4448); 1:1000 α-Centrobin (Proteintech 26880-1-AP); 1:10,000 α-CEP131 (the generous gift of J. Reiter, Department of Biochemistry and Biophysics, University of California, San Francisco School of Medicine, San Francisco, CA); 1:2000 α-PCM1 (Bethyl A301-150A) α-α-tubulin (Sigma DM1A); 1:50 α-KATNB1 (Proteintech 14969-1-AP).

Techniques: Microscopy, Expressing, Staining, Fluorescence, Standard Deviation