sars Search Results


spike b cell analysis kit  (Miltenyi Biotec)
97
Miltenyi Biotec spike b cell analysis kit
Spike B Cell Analysis Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sars/pmc09295666__jitc___2022___004953supp002-38-14-19?v=Miltenyi+Biotec
Average 97 stars, based on 1 article reviews
spike b cell analysis kit - by Bioz Stars, 2026-08
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adquirido de la casa comercial new england biolabs  (New England Biolabs)
96
New England Biolabs adquirido de la casa comercial new england biolabs
Detección colorimétrica de SARS-CoV-2 por RT-LAMP; empleando dos sets de cebadores, Broughton (A) y Lalli (B). Se recolectaron 117 muestras de ARN purificado a partir de hisopado nasofaríngeo de individuos sintomáticos para COVID-19. La reacción fue ejecutada a una temperatura de 65 °C durante 30 minutos, usando el estuche <t>comercial</t> Warmstart colorimetric LAMP 2X Master Mix (DNA & RNA) (NEB) en 20 μΙ de volumen final. La reacción de RT-LAMP tenía como diana el gen N del SARS-CoV-2. Las muestras negativas para SARS-CoV-2 se muestran en color rosa y las muestras positivas se visualizan por un cambio de color de rosa a amarillo. Los amplicones producto de la reacción de amplificación fueron separados en un gel de agarosa al 2 % y teñidos con SYBR Safe (Invitrogen). El patrón de tipo escalera observado en las muestras positivas, es coherente con una amplificación positiva y el subsecuente viraje de color en las muestras con presencia del SARS-CoV-2, en tanto que los productos de amplificación están ausentes en las muestras negativas. CP: fragmento sintético del gen N del SASR-CoV-2. CN: reacciones incubadas con agua de grado para biología molecular en lugar de ARN.
Adquirido De La Casa Comercial New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sars/pmc09683688-67-22-27?v=New+England+Biolabs
Average 96 stars, based on 1 article reviews
adquirido de la casa comercial new england biolabs - by Bioz Stars, 2026-08
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sars positive  (Xiaomi Inc)
86
Xiaomi Inc sars positive
Detección colorimétrica de SARS-CoV-2 por RT-LAMP; empleando dos sets de cebadores, Broughton (A) y Lalli (B). Se recolectaron 117 muestras de ARN purificado a partir de hisopado nasofaríngeo de individuos sintomáticos para COVID-19. La reacción fue ejecutada a una temperatura de 65 °C durante 30 minutos, usando el estuche <t>comercial</t> Warmstart colorimetric LAMP 2X Master Mix (DNA & RNA) (NEB) en 20 μΙ de volumen final. La reacción de RT-LAMP tenía como diana el gen N del SARS-CoV-2. Las muestras negativas para SARS-CoV-2 se muestran en color rosa y las muestras positivas se visualizan por un cambio de color de rosa a amarillo. Los amplicones producto de la reacción de amplificación fueron separados en un gel de agarosa al 2 % y teñidos con SYBR Safe (Invitrogen). El patrón de tipo escalera observado en las muestras positivas, es coherente con una amplificación positiva y el subsecuente viraje de color en las muestras con presencia del SARS-CoV-2, en tanto que los productos de amplificación están ausentes en las muestras negativas. CP: fragmento sintético del gen N del SASR-CoV-2. CN: reacciones incubadas con agua de grado para biología molecular en lugar de ARN.
Sars Positive, supplied by Xiaomi Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sars/pmc12224150__ac5c01487_si_001-126-148-152?v=Xiaomi+Inc
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sars positive - by Bioz Stars, 2026-08
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human igg1 as35  (ACROBiosystems)
95
ACROBiosystems human igg1 as35
Figure 3. SARS-CoV-2 <t>IgG</t> and neutralizing antibody responses (A and B) Serum IgG titers against wild-type SARS- CoV-2 RBD protein (A) and pseudovirus neutralizing antibody titers (ID50) against SARS-CoV-2 variants (B) for non-elderly (left side) and elderly (right side) participants (n = 10 per dose group, and n = 6 for the placebo group). Participants received one injection of VLPCOV-01 (0.3, 1.0, or 3.0 mg), 30 mg BNT162b2, or placebo on day 1 (week 0). Logarithmic values are reported as geometric mean titers for serum IgG and neutralizing antibody against pseudovirus. Bars indicate 95% CIs. (C) The correlation between serum neutralizing antibody titers against pseudovirus Wuhan (wild type) and IgG antibody titers against SARS-CoV-2 RBD protein following booster vaccination. Pearson’s product-moment correlation coefficient and p value were calculated following log trans- formation of source data (r = 0.950, p < 0.001).
Human Igg1 As35, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sars/pm37586325-110-168-171?v=ACROBiosystems
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mouse anti sars cov 2 n monoclonal antibody  (R&D Systems)
95
R&D Systems mouse anti sars cov 2 n monoclonal antibody
Figure 3. SARS-CoV-2 <t>IgG</t> and neutralizing antibody responses (A and B) Serum IgG titers against wild-type SARS- CoV-2 RBD protein (A) and pseudovirus neutralizing antibody titers (ID50) against SARS-CoV-2 variants (B) for non-elderly (left side) and elderly (right side) participants (n = 10 per dose group, and n = 6 for the placebo group). Participants received one injection of VLPCOV-01 (0.3, 1.0, or 3.0 mg), 30 mg BNT162b2, or placebo on day 1 (week 0). Logarithmic values are reported as geometric mean titers for serum IgG and neutralizing antibody against pseudovirus. Bars indicate 95% CIs. (C) The correlation between serum neutralizing antibody titers against pseudovirus Wuhan (wild type) and IgG antibody titers against SARS-CoV-2 RBD protein following booster vaccination. Pearson’s product-moment correlation coefficient and p value were calculated following log trans- formation of source data (r = 0.950, p < 0.001).
Mouse Anti Sars Cov 2 N Monoclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti sars cov 2 n monoclonal antibody - by Bioz Stars, 2026-08
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anti sars cov 2 neutralizing antibody titer serological assay kit  (ACROBiosystems)
95
ACROBiosystems anti sars cov 2 neutralizing antibody titer serological assay kit
Figure 3. SARS-CoV-2 <t>IgG</t> and neutralizing antibody responses (A and B) Serum IgG titers against wild-type SARS- CoV-2 RBD protein (A) and pseudovirus neutralizing antibody titers (ID50) against SARS-CoV-2 variants (B) for non-elderly (left side) and elderly (right side) participants (n = 10 per dose group, and n = 6 for the placebo group). Participants received one injection of VLPCOV-01 (0.3, 1.0, or 3.0 mg), 30 mg BNT162b2, or placebo on day 1 (week 0). Logarithmic values are reported as geometric mean titers for serum IgG and neutralizing antibody against pseudovirus. Bars indicate 95% CIs. (C) The correlation between serum neutralizing antibody titers against pseudovirus Wuhan (wild type) and IgG antibody titers against SARS-CoV-2 RBD protein following booster vaccination. Pearson’s product-moment correlation coefficient and p value were calculated following log trans- formation of source data (r = 0.950, p < 0.001).
Anti Sars Cov 2 Neutralizing Antibody Titer Serological Assay Kit, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sars/med_rxiv__2025__08__01__25332666-47-1-8?v=ACROBiosystems
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anti sars cov 2 neutralizing antibody titer serological assay kit - by Bioz Stars, 2026-08
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catalog number 10523 cv 100  (R&D Systems)
93
R&D Systems catalog number 10523 cv 100
Figure 3. SARS-CoV-2 <t>IgG</t> and neutralizing antibody responses (A and B) Serum IgG titers against wild-type SARS- CoV-2 RBD protein (A) and pseudovirus neutralizing antibody titers (ID50) against SARS-CoV-2 variants (B) for non-elderly (left side) and elderly (right side) participants (n = 10 per dose group, and n = 6 for the placebo group). Participants received one injection of VLPCOV-01 (0.3, 1.0, or 3.0 mg), 30 mg BNT162b2, or placebo on day 1 (week 0). Logarithmic values are reported as geometric mean titers for serum IgG and neutralizing antibody against pseudovirus. Bars indicate 95% CIs. (C) The correlation between serum neutralizing antibody titers against pseudovirus Wuhan (wild type) and IgG antibody titers against SARS-CoV-2 RBD protein following booster vaccination. Pearson’s product-moment correlation coefficient and p value were calculated following log trans- formation of source data (r = 0.950, p < 0.001).
Catalog Number 10523 Cv 100, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sars/pmc12192683-225-17-15?v=R%26D+Systems
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catalog number 10523 cv 100 - by Bioz Stars, 2026-08
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alexa 647 conjugated mouse anti sars cov 2 spike s1 subunit mab  (R&D Systems)
93
R&D Systems alexa 647 conjugated mouse anti sars cov 2 spike s1 subunit mab
Figure 3. SARS-CoV-2 <t>IgG</t> and neutralizing antibody responses (A and B) Serum IgG titers against wild-type SARS- CoV-2 RBD protein (A) and pseudovirus neutralizing antibody titers (ID50) against SARS-CoV-2 variants (B) for non-elderly (left side) and elderly (right side) participants (n = 10 per dose group, and n = 6 for the placebo group). Participants received one injection of VLPCOV-01 (0.3, 1.0, or 3.0 mg), 30 mg BNT162b2, or placebo on day 1 (week 0). Logarithmic values are reported as geometric mean titers for serum IgG and neutralizing antibody against pseudovirus. Bars indicate 95% CIs. (C) The correlation between serum neutralizing antibody titers against pseudovirus Wuhan (wild type) and IgG antibody titers against SARS-CoV-2 RBD protein following booster vaccination. Pearson’s product-moment correlation coefficient and p value were calculated following log trans- formation of source data (r = 0.950, p < 0.001).
Alexa 647 Conjugated Mouse Anti Sars Cov 2 Spike S1 Subunit Mab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sars/pmc11762317-251-0-34?v=R%26D+Systems
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alexa 647 conjugated mouse anti sars cov 2 spike s1 subunit mab - by Bioz Stars, 2026-08
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nucleocapsid protein  (Novus Biologicals)
88
Novus Biologicals nucleocapsid protein
Figure 3. SARS-CoV-2 <t>IgG</t> and neutralizing antibody responses (A and B) Serum IgG titers against wild-type SARS- CoV-2 RBD protein (A) and pseudovirus neutralizing antibody titers (ID50) against SARS-CoV-2 variants (B) for non-elderly (left side) and elderly (right side) participants (n = 10 per dose group, and n = 6 for the placebo group). Participants received one injection of VLPCOV-01 (0.3, 1.0, or 3.0 mg), 30 mg BNT162b2, or placebo on day 1 (week 0). Logarithmic values are reported as geometric mean titers for serum IgG and neutralizing antibody against pseudovirus. Bars indicate 95% CIs. (C) The correlation between serum neutralizing antibody titers against pseudovirus Wuhan (wild type) and IgG antibody titers against SARS-CoV-2 RBD protein following booster vaccination. Pearson’s product-moment correlation coefficient and p value were calculated following log trans- formation of source data (r = 0.950, p < 0.001).
Nucleocapsid Protein, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sars/pmc08340595-136-22-24?v=Novus+Biologicals
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sars cov 2 nucleocapsid  (Novus Biologicals)
93
Novus Biologicals sars cov 2 nucleocapsid
Figure 3. SARS-CoV-2 <t>IgG</t> and neutralizing antibody responses (A and B) Serum IgG titers against wild-type SARS- CoV-2 RBD protein (A) and pseudovirus neutralizing antibody titers (ID50) against SARS-CoV-2 variants (B) for non-elderly (left side) and elderly (right side) participants (n = 10 per dose group, and n = 6 for the placebo group). Participants received one injection of VLPCOV-01 (0.3, 1.0, or 3.0 mg), 30 mg BNT162b2, or placebo on day 1 (week 0). Logarithmic values are reported as geometric mean titers for serum IgG and neutralizing antibody against pseudovirus. Bars indicate 95% CIs. (C) The correlation between serum neutralizing antibody titers against pseudovirus Wuhan (wild type) and IgG antibody titers against SARS-CoV-2 RBD protein following booster vaccination. Pearson’s product-moment correlation coefficient and p value were calculated following log trans- formation of source data (r = 0.950, p < 0.001).
Sars Cov 2 Nucleocapsid, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sars/pmc10835285-85-12-21?v=Novus+Biologicals
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rabbit anti sars cov 2 spike primary antibodies  (Sino Biological)
96
Sino Biological rabbit anti sars cov 2 spike primary antibodies
Figure 3. SARS-CoV-2 <t>IgG</t> and neutralizing antibody responses (A and B) Serum IgG titers against wild-type SARS- CoV-2 RBD protein (A) and pseudovirus neutralizing antibody titers (ID50) against SARS-CoV-2 variants (B) for non-elderly (left side) and elderly (right side) participants (n = 10 per dose group, and n = 6 for the placebo group). Participants received one injection of VLPCOV-01 (0.3, 1.0, or 3.0 mg), 30 mg BNT162b2, or placebo on day 1 (week 0). Logarithmic values are reported as geometric mean titers for serum IgG and neutralizing antibody against pseudovirus. Bars indicate 95% CIs. (C) The correlation between serum neutralizing antibody titers against pseudovirus Wuhan (wild type) and IgG antibody titers against SARS-CoV-2 RBD protein following booster vaccination. Pearson’s product-moment correlation coefficient and p value were calculated following log trans- formation of source data (r = 0.950, p < 0.001).
Rabbit Anti Sars Cov 2 Spike Primary Antibodies, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti sars cov 2 spike primary antibodies - by Bioz Stars, 2026-08
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recombinant nsp10 his  (R&D Systems)
94
R&D Systems recombinant nsp10 his
Figure 3. SARS-CoV-2 <t>IgG</t> and neutralizing antibody responses (A and B) Serum IgG titers against wild-type SARS- CoV-2 RBD protein (A) and pseudovirus neutralizing antibody titers (ID50) against SARS-CoV-2 variants (B) for non-elderly (left side) and elderly (right side) participants (n = 10 per dose group, and n = 6 for the placebo group). Participants received one injection of VLPCOV-01 (0.3, 1.0, or 3.0 mg), 30 mg BNT162b2, or placebo on day 1 (week 0). Logarithmic values are reported as geometric mean titers for serum IgG and neutralizing antibody against pseudovirus. Bars indicate 95% CIs. (C) The correlation between serum neutralizing antibody titers against pseudovirus Wuhan (wild type) and IgG antibody titers against SARS-CoV-2 RBD protein following booster vaccination. Pearson’s product-moment correlation coefficient and p value were calculated following log trans- formation of source data (r = 0.950, p < 0.001).
Recombinant Nsp10 His, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Detección colorimétrica de SARS-CoV-2 por RT-LAMP; empleando dos sets de cebadores, Broughton (A) y Lalli (B). Se recolectaron 117 muestras de ARN purificado a partir de hisopado nasofaríngeo de individuos sintomáticos para COVID-19. La reacción fue ejecutada a una temperatura de 65 °C durante 30 minutos, usando el estuche comercial Warmstart colorimetric LAMP 2X Master Mix (DNA & RNA) (NEB) en 20 μΙ de volumen final. La reacción de RT-LAMP tenía como diana el gen N del SARS-CoV-2. Las muestras negativas para SARS-CoV-2 se muestran en color rosa y las muestras positivas se visualizan por un cambio de color de rosa a amarillo. Los amplicones producto de la reacción de amplificación fueron separados en un gel de agarosa al 2 % y teñidos con SYBR Safe (Invitrogen). El patrón de tipo escalera observado en las muestras positivas, es coherente con una amplificación positiva y el subsecuente viraje de color en las muestras con presencia del SARS-CoV-2, en tanto que los productos de amplificación están ausentes en las muestras negativas. CP: fragmento sintético del gen N del SASR-CoV-2. CN: reacciones incubadas con agua de grado para biología molecular en lugar de ARN.

Journal: Biomédica

Article Title: Validación clínica de la prueba RT-LAMP para el diagnóstico rápido del SARS-CoV-2

doi: 10.7705/biomedica.6523

Figure Lengend Snippet: Detección colorimétrica de SARS-CoV-2 por RT-LAMP; empleando dos sets de cebadores, Broughton (A) y Lalli (B). Se recolectaron 117 muestras de ARN purificado a partir de hisopado nasofaríngeo de individuos sintomáticos para COVID-19. La reacción fue ejecutada a una temperatura de 65 °C durante 30 minutos, usando el estuche comercial Warmstart colorimetric LAMP 2X Master Mix (DNA & RNA) (NEB) en 20 μΙ de volumen final. La reacción de RT-LAMP tenía como diana el gen N del SARS-CoV-2. Las muestras negativas para SARS-CoV-2 se muestran en color rosa y las muestras positivas se visualizan por un cambio de color de rosa a amarillo. Los amplicones producto de la reacción de amplificación fueron separados en un gel de agarosa al 2 % y teñidos con SYBR Safe (Invitrogen). El patrón de tipo escalera observado en las muestras positivas, es coherente con una amplificación positiva y el subsecuente viraje de color en las muestras con presencia del SARS-CoV-2, en tanto que los productos de amplificación están ausentes en las muestras negativas. CP: fragmento sintético del gen N del SASR-CoV-2. CN: reacciones incubadas con agua de grado para biología molecular en lugar de ARN.

Article Snippet: Como control positivo, en las reacciones de RT-LAMP se incluyó un plásmido que tiene clonado un fragmento del gen N del SARS-CoV-2, adquirido de la casa comercial New England Biolabs (NEB, Germany).

Techniques:

Detección colorimétrica de SARS-CoV-2 por RT-LAMP en muestras de hisopado nasofaríngeo, sin previa extracción de ARN. Se precalentaron 36 muestras de hisopado nasofaríngeo durante 5 minutos a 95 °C; posteriormente, se agregaron 3 μl de muestra a la reacción RT-LAMP usando el estuche comercial Warmstart colorimetric LAMP 2X Master Mix (DNA & RNA) (NEB) en 20 μl de volumen final. La RT-LAMP tenía como diana el gen N del SASR-CoV-2. Las muestras negativas para este virus se muestran en color rosa y las muestras positivas se visualizan por un cambio de color de rosa a amarillo. Los amplicones producto de la reacción de amplificación fueron resueltos en un gel de agarosa al 2 % y teñidos con SYBR Safe (Invitrogen). Se muestra el patrón típico de amplificación de la técnica RT- LAMP en muestras positivas y el cambio de color en la reacción; en muestras negativas, la mezcla reactiva permanece de color rosa y no se observan bandas en el gel electroforético. CP: fragmento sintético del gen N del SASR-CoV-2. CN: reacciones incubadas con agua de grado para biología molecular en lugar de muestras de hisopado.

Journal: Biomédica

Article Title: Validación clínica de la prueba RT-LAMP para el diagnóstico rápido del SARS-CoV-2

doi: 10.7705/biomedica.6523

Figure Lengend Snippet: Detección colorimétrica de SARS-CoV-2 por RT-LAMP en muestras de hisopado nasofaríngeo, sin previa extracción de ARN. Se precalentaron 36 muestras de hisopado nasofaríngeo durante 5 minutos a 95 °C; posteriormente, se agregaron 3 μl de muestra a la reacción RT-LAMP usando el estuche comercial Warmstart colorimetric LAMP 2X Master Mix (DNA & RNA) (NEB) en 20 μl de volumen final. La RT-LAMP tenía como diana el gen N del SASR-CoV-2. Las muestras negativas para este virus se muestran en color rosa y las muestras positivas se visualizan por un cambio de color de rosa a amarillo. Los amplicones producto de la reacción de amplificación fueron resueltos en un gel de agarosa al 2 % y teñidos con SYBR Safe (Invitrogen). Se muestra el patrón típico de amplificación de la técnica RT- LAMP en muestras positivas y el cambio de color en la reacción; en muestras negativas, la mezcla reactiva permanece de color rosa y no se observan bandas en el gel electroforético. CP: fragmento sintético del gen N del SASR-CoV-2. CN: reacciones incubadas con agua de grado para biología molecular en lugar de muestras de hisopado.

Article Snippet: Como control positivo, en las reacciones de RT-LAMP se incluyó un plásmido que tiene clonado un fragmento del gen N del SARS-CoV-2, adquirido de la casa comercial New England Biolabs (NEB, Germany).

Techniques: Virus

Figure 3. SARS-CoV-2 IgG and neutralizing antibody responses (A and B) Serum IgG titers against wild-type SARS- CoV-2 RBD protein (A) and pseudovirus neutralizing antibody titers (ID50) against SARS-CoV-2 variants (B) for non-elderly (left side) and elderly (right side) participants (n = 10 per dose group, and n = 6 for the placebo group). Participants received one injection of VLPCOV-01 (0.3, 1.0, or 3.0 mg), 30 mg BNT162b2, or placebo on day 1 (week 0). Logarithmic values are reported as geometric mean titers for serum IgG and neutralizing antibody against pseudovirus. Bars indicate 95% CIs. (C) The correlation between serum neutralizing antibody titers against pseudovirus Wuhan (wild type) and IgG antibody titers against SARS-CoV-2 RBD protein following booster vaccination. Pearson’s product-moment correlation coefficient and p value were calculated following log trans- formation of source data (r = 0.950, p < 0.001).

Journal: Cell reports. Medicine

Article Title: Safety and immunogenicity of SARS-CoV-2 self-amplifying RNA vaccine expressing an anchored RBD: A randomized, observer-blind phase 1 study.

doi: 10.1016/j.xcrm.2023.101134

Figure Lengend Snippet: Figure 3. SARS-CoV-2 IgG and neutralizing antibody responses (A and B) Serum IgG titers against wild-type SARS- CoV-2 RBD protein (A) and pseudovirus neutralizing antibody titers (ID50) against SARS-CoV-2 variants (B) for non-elderly (left side) and elderly (right side) participants (n = 10 per dose group, and n = 6 for the placebo group). Participants received one injection of VLPCOV-01 (0.3, 1.0, or 3.0 mg), 30 mg BNT162b2, or placebo on day 1 (week 0). Logarithmic values are reported as geometric mean titers for serum IgG and neutralizing antibody against pseudovirus. Bars indicate 95% CIs. (C) The correlation between serum neutralizing antibody titers against pseudovirus Wuhan (wild type) and IgG antibody titers against SARS-CoV-2 RBD protein following booster vaccination. Pearson’s product-moment correlation coefficient and p value were calculated following log trans- formation of source data (r = 0.950, p < 0.001).

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human CD154-FITC (clone: TRAP1) BD Biosciences cat# 555699; RRID: AB_396049 Mouse anti-human CD3-BUV615 (clone: SP34-2) BD Biosciences cat# 751249; RRID: AB_2875266 Mouse anti-human CD4-PE-Cy5.5 (clone: S3.5) Thermo Ficher Scientific cat# MHCD0418; RRID: AB_10376013 Mouse anti-human CD8-BUV563 (clone: RPA-T8) BD Biosciences cat# 612914; RRID: AB_2870200 Mouse anti-human CD27-PE-Cy5 (clone: 1A4CD27) Beckman coulter cat# 6607107 Mouse anti-human CD45RO-BUV805 (clone: UCHL1) BD Biosciences cat# 748367; RRID: AB_2872786 Mouse anti-human IFN-g-BV786 (clone: 4S.B3) BioLegend cat# 502542; RRID: AB_2563882 Mouse anti-human TNF-BV650 (clone: MAb11) BioLegend cat# 502938; RRID: AB_2562741 Rat anti-human IL-13-BV421 (clone: JES10-5A2) BD Biosciences cat# 563580; RRID: AB_2738290 Mouse anti-human IL-21-Ax647 (clone: 3A3-N21) BD Biosciences cat# 560493; RRID: AB_1645421 Mouse anti-human IL-4-PE-Cy7 (clone:8D4-8) BD Biosciences cat# 560672; RRID: AB_1727547 Mouse anti-human IL-17A-BV605 (clone: BL168) BioLegend cat# 512326; RRID: AB_2563887 Rat anti-human IL-2-BUV737 (clone: MQ117H12) BD Biosciences cat# 612836 Mouse anti-human CD107A-BV711 (clone: H4A3) BioLegend cat# 328640; RRID: AB_2565840 Mouse anti-human MIP1b-Alexa700 (clone: D21-1351) BD Biosciences cat# 561278; RRID: AB_10612008 Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1 (AS35) ACROBiosystems cat# SAD-S35 Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG2 (AS35) ACROBiosystems cat# SAD-S66 Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG3 (AS35) ACROBiosystems cat# SAD-S67 Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG4 (AS35) ACROBiosystems cat# SAD-S68 Mouse anti-Human IgG1 Fc Secondary Antibody, HRP Thermo Fisher Scientific cat# MH1715; RRID: AB_2539710 Mouse Anti-Human IgG2 Fc-BIOT HP6002 SouthernBiotech cat# 9070-08; RRID: AB_2796638 Mouse Anti-Human IgG3 Hinge-BIOT HP6050 SouthernBiotech cat# 9210-08; RRID: AB_2796700 Mouse Anti-Human IgG4 Fc-BIOT HP6025 SouthernBiotech cat# 9200-08; RRID: AB_2796692 Biological samples Human PBMCs This study This study Chemicals, peptides, and recombinant proteins Benzonase Nuclease, Purity >90% MERCK Millipore cat# 70746 (Continued on next page) Cell Reports Medicine 4, 101134, August 15, 2023 e1

Techniques: Injection