Journal: Journal of AOAC International

Article Title: Validation of the 3M™ Petrifilm™ Coliform Count Plate for Enumeration of Coliforms in Bottled Water: AOAC Performance Tested Method SM 082101

doi: 10.1093/jaoacint/qsab137

Figure Lengend Snippet: Inclusivity results for 3M Petrifilm CC Plates—bottled water method

Article Snippet: 40 , Klebsiella oxytoca , ATCC 43086 , Unknown , G, G+ , NA.

Techniques:

Journal: bioRxiv

Article Title: Cancer-associated KBTBD4 mutations induce differentiation defects and confer a unique therapeutic vulnerability

doi: 10.64898/2026.03.12.711277

Figure Lengend Snippet: (A) Volcano plot showing global protein expression in HL60 cells transduced with P311PP mutant. Blue and red dots show significantly decreased or increased proteins (adjusted p-value<0.05). CoREST components RREB1, RCOR1, KDM1A, RCOR3, and ZNF217 are highlighted in the volcano plot. The global proteome data was plotted after excluding PCDHGA11. (B) High throughput compound screening in HL60 cells stably expressing RCOR1-GFP treated with UM171 (200nM). Schematic of the screening rationale (top panel). The compounds were added at a final concentration of 1µM, and flow analysis was performed after 3 hours post treatment to measure the relative GFP expression (bottom panel). Red dots show the hits from the screen and the table on right-side shows the functional classification of the hits. (C) A dose-titration experiment showing the rescue of RCOR1-GFP by two class I specific HDAC inhibitors (mocetinostat and romidepsin) and three Pan HDAC inhibitors (belinostat, pracinostat and vorinostat). GFP mean fluorescence intensity of DMSO treatment was used as controls. Data from 3 replicates from 1 of 2 independent experiments with similar results are shown. (D) RCOR1 protein levels in P311PP expressing HL60 cells either treated with DMSO, belinostat (320nM) or mocetinostat (300nM) for 3 hours. (E) RCOR1 ELM2-GFP clones were expressed in HL60 cells and analyzed for the interaction with HDAC2 through immunoprecipitation. The degradation profiles of the corresponding alanine substitution clones to UM171 treatment are represented as a heat map. (F) Schematic representation of the mechanistic basis of HDAC inhibitors in preventing the CoREST degradation.

Article Snippet: Belinostat (#HY-10225), Vorinostat (#HY-10221), and Mocetinostat (#HY-12164) were purchased from MedChemExpress.

Techniques: Expressing, Transduction, Mutagenesis, High Throughput Screening Assay, Stable Transfection, Concentration Assay, Functional Assay, Titration, Fluorescence, Clone Assay, Immunoprecipitation

Journal: bioRxiv

Article Title: Cancer-associated KBTBD4 mutations induce differentiation defects and confer a unique therapeutic vulnerability

doi: 10.64898/2026.03.12.711277

Figure Lengend Snippet: (A) FACS plots showing lineage output in WT and R313PRR expressing HSPCs treated with either DMSO or mocetinostat (250nM) for 5 days. (B) Summary of the lineage output for DMSO, belinostat (125nM) and mocetinostat (250nM) treatments. Data from 3 replicates from 1 of 4 independent experiments with similar results are shown. (C) Heat map demonstrating ELM2-GFP expression profiles in HL60 cells overexpressing KBTBD4 mutants treated with DMSO or mocetinostat (75nM) for 24 hours. Data analyzed at 96 hours post transduction. Statistical analyses were performed in comparison to the WT KBTBD4 treated with DMSO.

Article Snippet: Belinostat (#HY-10225), Vorinostat (#HY-10221), and Mocetinostat (#HY-12164) were purchased from MedChemExpress.

Techniques: Expressing, Transduction, Comparison

Journal: PLoS ONE

Article Title: Histone Deacetylase Inhibitors Antagonize Distinct Pathways to Suppress Tumorigenesis of Embryonal Rhabdomyosarcoma

doi: 10.1371/journal.pone.0144320

Figure Lengend Snippet: (A) Western blots demonstrating hyperacetylation of histones using antibodies against acetyl-histone H3 (Lys9), acetyl-histone H3 (Lys27), acetyl-histone H4 (Lys5), and acetyl-histone H4 (Lys8). D: DMSO; S: vorinostat (SAHA); T: TSA. Each band intensity was normalized to GAPDH loading control. Relative fold increase to DMSO (vehicle) treatment is shown. (B) Analysis of cell viability by cell counts. Cell counts were performed on cells treated with DMSO, 200 nM TSA or 1 μM SAHA at day 0 and day 5. Fold change in cell counts was normalized to day 0. (C-D) Representative images of immunofluorescence (IF) against MF20 performed on RD cells treated with DMSO (C) or 200 nM TSA (D) for 3 days in 2% horse serum in DMEM. Green: MF20-positive cells. Blue: DAPI. Scale bar: 20 μm. (E) Summary of IF against MF20 in ERMS (RD, 381T and SMS-CTR) and ARMS (Rh3, Rh5 and Rh30) cell lines treated with DMSO, 200 nM TSA or 1 μM SAHA. (F) Chromatin immunoprecipitation (ChIP) assays showing differential binding of acetyl-histone H3 (Lys9) at myogenic promoters. Fold enrichment binding of MYOD1 , MYOG , and MYH4 promoter regions were determined by quantitative PCR, normalizing amplification levels to input DNA of each sample. Rabbit IgG was used as a negative control for chromatin immunoprecipitation. (G-H) Representative bright-field images from a sphere assay on RD cells treated with DMSO (G) and 200 nM TSA (H). (I) Summary of sphere assays in RD and 381T cells. Each error bar in panels (B), (E), (F) and (I) indicates standard deviation of 3 technical replicates. * indicates p < 0.05. ** indicates p < 0.01. *** indicates p < 0.001.

Article Snippet: In the differentiation assay, cells were cultured in 2% horse serum and treated with TSA or SAHA for 3 days prior to fixation and immunostaining for MF20 (1:200; R&D Systems) and DAPI (1:500; Life Technologies).

Techniques: Western Blot, Control, Immunofluorescence, Chromatin Immunoprecipitation, Binding Assay, Real-time Polymerase Chain Reaction, Amplification, Negative Control, Standard Deviation

Journal: PLoS ONE

Article Title: Histone Deacetylase Inhibitors Antagonize Distinct Pathways to Suppress Tumorigenesis of Embryonal Rhabdomyosarcoma

doi: 10.1371/journal.pone.0144320

Figure Lengend Snippet: (A-F) Scratch assays on RD cells treated with DMSO, 1 μM SAHA or 200 nM TSA. (A-C) Representative images at time of scratch (0 hr). (D-F) Representative images at 18 hours post-scratch. Scale bar = 500 μm. (G) Summary of scratch assays in RD cells, indicating % wound closure for each treatment. (H-J) Representative images post-22 hour migration from transwell assays on RD cells treated with DMSO, 1 μM SAHA or 200 nM TSA. Scale bar = 50 μm. (K) Summary of transwell assays. Each error bar in panels (G) and (K) indicates standard deviation from technical triplicates. * indicates p < 0.05.

Article Snippet: In the differentiation assay, cells were cultured in 2% horse serum and treated with TSA or SAHA for 3 days prior to fixation and immunostaining for MF20 (1:200; R&D Systems) and DAPI (1:500; Life Technologies).

Techniques: Migration, Standard Deviation

Journal: PLoS ONE

Article Title: Histone Deacetylase Inhibitors Antagonize Distinct Pathways to Suppress Tumorigenesis of Embryonal Rhabdomyosarcoma

doi: 10.1371/journal.pone.0144320

Figure Lengend Snippet: (A) Western blots demonstrating hyperacetylation of histone H3 (Lys9) and histone H4 (Lys5) in zebrafish ERMS treated with 1 μM TSA or 50 μM SAHA. GAPDH was used as loading control. The values shown represent fold change in band intensity from TSA or SAHA treatment relative to DMSO after normalizing to loading control. (B) Representative pre- and post-treatment images of zebrafish ERMS treated with DMSO (vehicle) or 1 μM TSA. Dotted line outlines the tumor in each fish. Scale bar = 2 mm. (C) Summary of tumor volume change of zebrafish Tg( myf5 :GFP; mylz2 :mCherry) ERMS treated with DMSO, 50 μM SAHA or 1 μM TSA. Overlaid images of bright field and red fluorescent channel are shown. Error bar indicates standard error of means. n = number of animals treated in each cohort. (D) Summary of quantitative Fluorescence Activated Cell Sorting analysis on ERMS treated with DMSO or 10 μM SAHA. Each pie chart shows relative proportion of each tumor cell subpopulation in an individual treated tumor. Green: myf5 :GFP + / mylz2 :mCherry − cells; yellow: myf5 :GFP + / mylz2 :mCherry + cells; red: mylz2 :mCherry + / myf5 :GFP − cells. (E) Quantitative RT-PCR analysis of myog and myod mRNA expression in ERMS treated with DMSO, 1 μM TSA or 50 μM SAHA. Each bar demonstrates an individual tumor. Each error bar indicates standard deviation of technical triplicates. (F) Annexin V analysis of ERMS tumors treated with DMSO, 50 μM SAHA, or 1μM TSA. 6 animals were analyzed per group. Each error bar indicates standard deviation. * indicates p < 0.05. ** indicates p < 0.01.

Article Snippet: In the differentiation assay, cells were cultured in 2% horse serum and treated with TSA or SAHA for 3 days prior to fixation and immunostaining for MF20 (1:200; R&D Systems) and DAPI (1:500; Life Technologies).

Techniques: Western Blot, Control, Fluorescence, FACS, Quantitative RT-PCR, Expressing, Standard Deviation

Journal: PLoS ONE

Article Title: Histone Deacetylase Inhibitors Antagonize Distinct Pathways to Suppress Tumorigenesis of Embryonal Rhabdomyosarcoma

doi: 10.1371/journal.pone.0144320

Figure Lengend Snippet: (A) Summary of MF20 IF of RD cells treated with two different doses of GSI-IX, PD173074, or cyclopamine. (B-C) Representative MF20 IF images of RD cells treated with DMSO (B) and 20 μM GSI-IX (C). Scale bar = 20 μm. (D) Western blot of NOTCH1 expression in RD cells treated with DMSO, 200 nM TSA, or 1 μM SAHA. Each band intensity was normalized to GAPDH loading control. Percent intensity relative to DMSO (vehicle) treatment is shown. (E) Quantitative RT-PCR analysis of NOTCH1 pathway downstream targets, HEY1 , HEY2 and HES1 in RD cells treated with DMSO, 200 nM TSA, or 1 μM SAHA. (F) ChIP assay summary showing differential binding of acetyl-histone H3 (Lys9) at the NOTCH1 promoter in RD cells treated with DMSO or 200 nM TSA. Rabbit IgG was used as a negative control for chromatin immunoprecipitation. (G) Summary of MF20 IF of control GFP-overexpressing and NICD-overexpressing RD cells treated with DMSO, 200 nM TSA or 1 μM SAHA. Error bar in each graph indicates standard deviation of technical triplicates. Brackets in each group are used to indicate comparison groups. * indicates p < 0.05. ** indicates p < 0.01.

Article Snippet: In the differentiation assay, cells were cultured in 2% horse serum and treated with TSA or SAHA for 3 days prior to fixation and immunostaining for MF20 (1:200; R&D Systems) and DAPI (1:500; Life Technologies).

Techniques: Western Blot, Expressing, Control, Quantitative RT-PCR, Binding Assay, Negative Control, Chromatin Immunoprecipitation, Standard Deviation, Comparison

Journal: PLoS ONE

Article Title: Histone Deacetylase Inhibitors Antagonize Distinct Pathways to Suppress Tumorigenesis of Embryonal Rhabdomyosarcoma

doi: 10.1371/journal.pone.0144320

Figure Lengend Snippet: Summary of (A) scratch assays and (B) transwell migration assays on RD and 381T cells with EFNB1 knockdown by two independent siRNAs. Summary of (C) scratch assays and (D) transwell migration assays performed on control GFP-overexpressing and EFNB1-overexpressing RD cell lines. (E) ChIP assays showing differential binding of acetyl-histone H3 (Lys9) on EFNB1 promoter in RD cells treated with DMSO, 200 nM TSA, or 1μM SAHA. Rabbit IgG was used as a negative control for chromatin immunoprecipitation. (F) Western blot assessing EFNB1 protein expression level in RD cells treated with DMSO, 200 nM TSA or 1 μM SAHA. Percent intensity relative to DMSO (vehicle) treatment is shown. GAPDH was used as a loading control.

Article Snippet: In the differentiation assay, cells were cultured in 2% horse serum and treated with TSA or SAHA for 3 days prior to fixation and immunostaining for MF20 (1:200; R&D Systems) and DAPI (1:500; Life Technologies).

Techniques: Migration, Knockdown, Control, Binding Assay, Negative Control, Chromatin Immunoprecipitation, Western Blot, Expressing

Journal: GeroScience

Article Title: Lung endothelial cell senescence impairs barrier function and promotes neutrophil adhesion and migration.

doi: 10.1007/s11357-025-01517-9

Figure Lengend Snippet: Fig. 5 Senescent ECs show increased permeability to solutes of different sizes. A Endothelial permeability to FITC-BSA (70-kD) in SAHA- or DOXO-treated ECs is higher than that of vehicle-treated cells. B Endothelial permeability to dextran

Article Snippet: In brief, ECs were treated with SAHA (4 μM; Tocris; catalog no. 4652) for 8 days (drug was refreshed daily) and control (non-senescent) cells were cultured in parallel and treated with identical volumes of vehicle DMSO.

Techniques: Permeability

Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: Combining three independent pathological stressors induces a heart failure with preserved ejection fraction phenotype

doi: 10.1152/ajpheart.00594.2022

Figure Lengend Snippet: The 3-Hit mouse model produces a profound heart failure with preserved ejection fraction (HFpEF) phenotype, which can be reduced by suberoylanilide hydroxamic acid (SAHA) treatment. β2a, transgenic mouse with low levels of cardiomyocyte (CM)-specific inducible Cavβ2a expression; HFD, high-fat diet; l -NAME (or LN), N ω -nitro- l -arginine methyl ester; N, normal diet; WT, wild type. Data shown for WT-N, WT-HFD-LN, β2a-N, and β2a-HFD-LN groups in are the same as those shown in and , and Supplemental Fig. S2. Statistical comparisons being made here are unique. The β2a-HFD-LN-SAHA group was added into the statistic comparation. A : survival rate from 4-mo follow-up. B : body weight (BW) at the time of euthanasia. Conventional and sophisticated echocardiography data showing left ventricular (LV) wall thickness ( C and D ), LV ejection fraction (LVEF; E ), LV longitudinal strain ( F ), and left atrium (LA) diameter ( G ). H : LA weight-to-BW ratio at the time of euthanasia. I : conventional echocardiography data showing ratio between early mitral inflow velocity ( E ) and mitral annular early diastolic velocity ( e′ ) ( E / e ′). Hemodynamics data showing end-diastolic pressure (EDP; J ) and LV diastolic time constant (τ; K ). L : expression level of atrial natriuretic peptide (ANP) in heart tissues by real-time polymerase chain reaction. Relative expression was calculated with respect to the WT-N group. HW, heart weight; LVAWd, end-diastolic left ventricular anterior wall thicknesses; LVPWd, end-diastolic left ventricular posterior wall thickness; TL, tibia length. Data shown are means ± SE. Tukey post hoc multiple comparison adjusted P values: C–G and I : P < 0.05, *WT-N vs. β2a-HFD-LN, #WT-N vs. β2a-N,@WT-N vs. WT-HFD-LN, &β2a-N vs. β2a-HFD-LN, and $WT-HFD-LN vs. β2a-HFD-LN; and other panels: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Total number of animals ( n ) and number of females and males included in each group are reported in the Supplemental Table.

Article Snippet: Both WT and β2a-Tg mice were housed in an animal room with a 12-h:12-h light/dark cycle from 6:00 am to 6:00 pm , a temperature of 22 ± 3°C, relative humidity of 50 ± 6%, and free access to food (Cat. No. 2916, Teklad for normal chow diet groups and D12492, Research Diet for the high-fat diet groups) and water [tap water, or N ω -nitro- l -arginine methyl ester; 0.5 g/L, Cayman Chemical], or l -NAME with suberoylanilide hydroxamic acid (SAHA, vorinostat, 670 mg/L, Biogems; 670 mg SAHA dissolved by 6.7 mL DMSO and 20 mL PGE300) (Detailed study groups provided in Supplemental Table S1).

Techniques: Transgenic Assay, Expressing, Real-time Polymerase Chain Reaction, Comparison

Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: Combining three independent pathological stressors induces a heart failure with preserved ejection fraction phenotype

doi: 10.1152/ajpheart.00594.2022

Figure Lengend Snippet: The 3-Hit mouse model has a more severe cardiomyocyte hypertrophy and necrosis, which can be reduced by suberoylanilide hydroxamic acid (SAHA) treatment. β2a, transgenic mouse with low levels of cardiomyocyte (CM)-specific inducible Cavβ2a expression; HFD, high-fat diet; N, normal chow diet, l -NAME (LN), N ω -nitro- l -arginine methyl ester; WT, wild type. Representative images of wheat germ agglutinin (WGA)-stained hearts ( C ) and quantification of cardiomyocyte cross-sectional area (CSA) data ( D ) in WT-N, WT-HFD-LN, β2a-N, and β2a-HFD-LN groups are the same as those shown in Fig. 1, and , and , and . The statistical comparisons being made here are different than those made in and . The β2a-HFD-LN-SAHA group was added into the statistic comparison. A and B : expression level of class I histone deacetylases (HDACs) 1, 2, 3 and 8, Class IIb HDAC6 ( A ) and class IIa HDACs 4 and 7 ( B ) in heart tissues by real-time polymerase chain reaction. Relative expression was calculated with respect to WT-N mice compared with different treatment groups. C : representative images of WGA-stained hearts and histological assessment of cardiac ventricular pathology by Von Kossa staining. D : quantification of cardiomyocyte CSA. E and F : representative images of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-stained hearts ( E ) and quantification of TUNEL-positive myocyte nuclei from hearts ( F ): α-sarcomeric actin (α-SA), red; TUNEL, green; and 4′,6-diamidino-2-phenylindole (DAPI), blue. Data shown are means ± SE. Tukey post hoc multiple comparison adjusted P values are reported here. * P < 0.05, ** P < 0.01, **** P < 0.0001.

Article Snippet: Both WT and β2a-Tg mice were housed in an animal room with a 12-h:12-h light/dark cycle from 6:00 am to 6:00 pm , a temperature of 22 ± 3°C, relative humidity of 50 ± 6%, and free access to food (Cat. No. 2916, Teklad for normal chow diet groups and D12492, Research Diet for the high-fat diet groups) and water [tap water, or N ω -nitro- l -arginine methyl ester; 0.5 g/L, Cayman Chemical], or l -NAME with suberoylanilide hydroxamic acid (SAHA, vorinostat, 670 mg/L, Biogems; 670 mg SAHA dissolved by 6.7 mL DMSO and 20 mL PGE300) (Detailed study groups provided in Supplemental Table S1).

Techniques: Transgenic Assay, Expressing, Staining, Comparison, Real-time Polymerase Chain Reaction, TUNEL Assay

Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: Combining three independent pathological stressors induces a heart failure with preserved ejection fraction phenotype

doi: 10.1152/ajpheart.00594.2022

Figure Lengend Snippet: The 3-Hit model has robust cardiac M 2 -macrophage infiltration and fibroblast activation, which can be reduced by suberoylanilide hydroxamic acid (SAHA) treatment. A : immunofluorescence staining of heart sections to show CD45 + (red) immune cell, CD45 + (red) CD68 + (green) macrophage, CD68 + (green) CD206 + (red) M 2 -macrophage, and phosphorylated Smad2/3 (pSmad2/3, green) in fibroblast (red). 4′,6-Diamidino-2-phenylindole (DAPI) was stained as blue. Data are expressed as percentage of CD45 + cell/total cells ( B ), percentage of CD68 + cell/CD45 + cell ( C ), and CD206 + CD68 + /total cell ( D ). E : expression level of transforming growth factor-β (TGFβ) in heart tissues by real-time polymerase chain reaction. Relative expression was calculated with respect to wild-type/normal chow diet (WT-N) mice compared with different treatment groups. F : quantification of mean intensity of pSmad2/3 for each group. β2a, transgenic mouse with low levels of cardiomyocyte (CM)-specific inducible Cavβ2a expression; HFD, high-fat diet; l -NAME (LN), N ω -nitro- l -arginine methyl ester; P4HB, protein disulfide isomerase/prolyl 4-hydroxylase. Data shown are means ± SE. Tukey post hoc multiple comparison adjusted P values are reported here. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Both WT and β2a-Tg mice were housed in an animal room with a 12-h:12-h light/dark cycle from 6:00 am to 6:00 pm , a temperature of 22 ± 3°C, relative humidity of 50 ± 6%, and free access to food (Cat. No. 2916, Teklad for normal chow diet groups and D12492, Research Diet for the high-fat diet groups) and water [tap water, or N ω -nitro- l -arginine methyl ester; 0.5 g/L, Cayman Chemical], or l -NAME with suberoylanilide hydroxamic acid (SAHA, vorinostat, 670 mg/L, Biogems; 670 mg SAHA dissolved by 6.7 mL DMSO and 20 mL PGE300) (Detailed study groups provided in Supplemental Table S1).

Techniques: Activation Assay, Immunofluorescence, Staining, Expressing, Real-time Polymerase Chain Reaction, Transgenic Assay, Comparison

Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: Combining three independent pathological stressors induces a heart failure with preserved ejection fraction phenotype

doi: 10.1152/ajpheart.00594.2022

Figure Lengend Snippet: The 3-Hit model has severe myocardial fibrosis, which can be reduced be suberoylanilide hydroxamic acid (SAHA) treatment. β2a, transgenic mouse with low levels of cardiomyocyte (CM)-specific inducible Cavβ2a expression; HFD, high-fat diet; N, normal chow diet, l -NAME (LN), ω -nitro- l -arginine methyl ester; WT, wild type. The representative images of Picrosirius red-stained hearts ( A ) and quantification of the percentage of Picrosirius red-positive area ( C ) data of WT-N, WT-HFD-LN, β2a-N, and β2a-HFD-LN groups are the same as in Fig. 1, and , and , and . The statistical comparisons being made are different than those made in and . The β2a-HFD-LN-SAHA group was added into the statistic comparation. A : representative images of hearts from 4 groups with α-smooth muscle actin (α-SMA) (red) immunofluorescence staining and Picrosirius red staining. Quantification of the α-SMA (red) intensity ( B ) and the percentage of Picrosirius red-positive area ( C ). Expression level of fibronectin 1 (FN1; D ) and collagen cross-linking enzyme lysyl oxidase (LOX; E ) in heart tissues by real-time polymerase chain reaction. Relative expression was calculated with respect to WT-N mice compared with different treatment groups. DAPI: 4′,6-diamidino-2-phenylindole. Data shown are means ± SE. Tukey post hoc multiple comparison adjusted P values are reported here. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Both WT and β2a-Tg mice were housed in an animal room with a 12-h:12-h light/dark cycle from 6:00 am to 6:00 pm , a temperature of 22 ± 3°C, relative humidity of 50 ± 6%, and free access to food (Cat. No. 2916, Teklad for normal chow diet groups and D12492, Research Diet for the high-fat diet groups) and water [tap water, or N ω -nitro- l -arginine methyl ester; 0.5 g/L, Cayman Chemical], or l -NAME with suberoylanilide hydroxamic acid (SAHA, vorinostat, 670 mg/L, Biogems; 670 mg SAHA dissolved by 6.7 mL DMSO and 20 mL PGE300) (Detailed study groups provided in Supplemental Table S1).

Techniques: Transgenic Assay, Expressing, Staining, Immunofluorescence, Real-time Polymerase Chain Reaction, Comparison

Journal:

Article Title: Detection of Legionellae in Hospital Water Samples by Quantitative Real-Time LightCycler PCR

doi: 10.1128/AEM.67.9.3985-3993.2001

Figure Lengend Snippet: 16S rRNA primer sequences and alignment of the primer target sequences

Article Snippet: For specificity control of the 16S rRNA gene PCR, the following bacteria were used (10 pg of bacterial DNA per PCR assay each): Acinetobacter junii (ATCC 17908), Acinetobacter baumannii (ATCC 19606), Acinetobacter lwoffii (ATCC 15309), Citrobacter freundii (ATCC 8090), Citrobacter koseri (ATCC 27028), Enterobacter aerogenes (ATCC 13048), Klebsiella oxytoca (ATCC 43863), Proteus mirabilis (ATCC 29906), Proteus vulgaris (ATCC 29905), Serratia marcescens (ATCC 13880), Stenotrophomonas maltophilia (ATCC 13637), Alcaligenes faecalis, Brevundimonas vesicularis , Chryseomonas luteola , Enterobacter cloacae , Escherichia coli , Klebsiella pneumoniae , Pseudomonas aeruginosa , Pseudomonas fluorescens , Pseudomonas putida , Sphingomonas paucimobilis, Streptococcus pyogenes, Staphylococcus aureus , and Enterococcus faecium (all clinical isolates). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Species and serogroup Strain or reference L. pneumophila 1 (Philadelphia 1) ATCC 33152 2 (Togus 1) ATCC 33154 3 (Bloomington 2) ATCC 33155 4 (Los Angeles 1) ATCC 33156 5 ATCC 33216 6 (Chicago 2) ATCC 33215 7 (Chicago 8) ATCC 33823 8 ATCC 35096 9 ATCC 35289 10 ATCC 43283 11 ATCC 43130 12 ATCC 43290 13 ATCC 43736 14 ATCC 43703 L. adelaidensis ATCC 49625 L. anisa ATCC 35292 L. birminghamensis ATCC 43702 L. bozemanii ATCC 33217 L. brunensis ATCC 43878 L. cherii ATCC 35252 L. cincinnatiensis ATCC 43753 L. dumoffii ATCC 33279 L. erythra ATCC 35303 L. feelei ATCC 35849 L. gormanii ATCC 33297 L. gratiana ATCC 49413 L. hackeliae ATCC 35250 L. israelensis ATCC 43119 L. jamestownensis ATCC 35298 L. jordanis ATCC 33623 L. lansingensis ATCC 49751 L. longbeachae ATCC 33462 L. maceachernii ATCC 35300 L. micdadei ATCC 33218 L. moravica ATCC 43877 L. oakridgensis ATCC 33761 L. parisiensis ATCC 35299 L. quinlivanii ATCC 43830 L. rubrilucens ATCC 35304 L. sainthelensis ATCC 35248 L. spiritensis ATCC 35249 L. steigerwaltii ATCC 35302 L. tucsonensis ATCC 49180 L. wadsworthii ATCC 33877 LLAP 10 1 Open in a separate window Legionella strains investigated by PCR Collection of hospital water samples.

Techniques:

Journal: Phage (New Rochelle, N.y.)

Article Title: Isolation and Characterization of Klebsiella Phages for Phage Therapy

doi: 10.1089/phage.2020.0046

Figure Lengend Snippet: Details of Klebsiella Species and Strains Used in This Study

Article Snippet: Klebsiella michiganensis , 25444 , O1v1 , × , DSMZ Culture Collection , Toothbrush holder.

Techniques: Isolation