Journal: Journal of AOAC International

Article Title: Validation of the 3M™ Petrifilm™ Coliform Count Plate for Enumeration of Coliforms in Bottled Water: AOAC Performance Tested Method SM 082101

doi: 10.1093/jaoacint/qsab137

Figure Lengend Snippet: Inclusivity results for 3M Petrifilm CC Plates—bottled water method

Article Snippet: 40 , Klebsiella oxytoca , ATCC 43086 , Unknown , G, G+ , NA.

Techniques:

Journal: Scientific Reports

Article Title: Single-cell analysis reveals IGF-1 potentiation of inhibition of the TGF-β/Smad pathway of fibrosis in human keratocytes in vitro

doi: 10.1038/srep34373

Figure Lengend Snippet: (a) Relative levels of Smad3 nuclearization in human keratocytes. *p < 0.001 compared to CON+; **p < 0.001 compared to CON+ and CON− (Chi 2 tests). (b) Workflow diagram of the IDEAS software. First, images of the keratocytes in focus were chosen, and then the relevant cell population was selected, excluding the nonspecific labeling. The probe similarity algorithm for co-localization of the nucleus and Smad3 was then defined. The software calculated the correlation coefficient (Similarity Dilate) of the two probes, plotted as a frequency histogram. R3, keratocytes with Similarity Dilate >1, which implies nuclearization has occurred. (c) Representative images of ketatocytes labeled for Smad3 and the nucleus, as revealed by the imaging flow cytometry (green, AlexaFluor® 488, Smad3; purple, 7AAD, nucleus). Upper panel, cytosolic Smad3; lower panel, nuclearized Smad3. CON+, positive control of 10 ng/ml TGF-β2; CON−, negative control for naïve human keratocytes. All of the following conditions also included 10 ng/ml TGF-β2: 10 ng/ml IGF-1; 5 ng/ml halofuginone (HAL); 10 ng/ml IGF-1 plus 5 ng/ml halofuginone (combo HAL); 10 nM SAHA; 10 ng/ml IGF-1 plus 10 nM SAHA (combo SAHA).

Article Snippet: The bioactives used were: 10 ng/ml, 5 ng/ml recombinant IGF-1 (R&D systems, Minneapolis, USA), 10 ng/ml, 5 ng/ml halofuginone (Santa Cruz Biotechnology, Heidelberg, Germany), and 10 nM, 5 nM SAHA (Tocris Bioscience, Bristol, UK).

Techniques: Software, Labeling, Imaging, Flow Cytometry, Positive Control, Negative Control

Journal: Scientific Reports

Article Title: Single-cell analysis reveals IGF-1 potentiation of inhibition of the TGF-β/Smad pathway of fibrosis in human keratocytes in vitro

doi: 10.1038/srep34373

Figure Lengend Snippet: As shown with imaging flow cytometry, IGF-1, SAHA and halofuginone block Smad3 translocation to the nucleus. Inhibitory Smad7 forms as a negative-feedback loop , blocking Smad3 phosphorylation and nuclearization. SAHA can also inhibit HDACs, which leads to acetylation and subsequent ubiquitination of Smad7 , resulting in higher intracellular Smad7 levels, as observed in the present study. P, phosphate group; UB, ubiquitin; HDAC, histone deacetylase.

Article Snippet: The bioactives used were: 10 ng/ml, 5 ng/ml recombinant IGF-1 (R&D systems, Minneapolis, USA), 10 ng/ml, 5 ng/ml halofuginone (Santa Cruz Biotechnology, Heidelberg, Germany), and 10 nM, 5 nM SAHA (Tocris Bioscience, Bristol, UK).

Techniques: Imaging, Flow Cytometry, Blocking Assay, Translocation Assay, Phospho-proteomics, Ubiquitin Proteomics, Histone Deacetylase Assay

Journal: Cell Reports Medicine

Article Title: Pharmacological Activation of Non-canonical NF-κB Signaling Activates Latent HIV-1 Reservoirs In Vivo

doi: 10.1016/j.xcrm.2020.100037

Figure Lengend Snippet: Ciapavir Does Not Induce Cytokine Release or T Cell Activation Human PBMCs and rCD4 + T cells from three healthy donors (n = 3) were treated with Ciapavir at the indicated concentrations, 500 nM vorinostat, 40 nM panobinostat, or combinations thereof, for 24 h. 50 ng/mL PMA and 1 μM ionomycin, or anti-CD3/CD28 antibody-coated beads, served as positive controls. (A and B) Heatmaps represent mean cytokine levels measured in the culture supernatant of PBMCs (A) or rCD4 + T cells (B) from tested donors (see Figure S2 for detailed results). (C and D) rCD4 + T cells treated as indicated were analyzed for CD69 (C) and CD25 (D) expression by flow cytometry. (E) Viability of rCD4 + T cells following treatment was assessed by measuring cellular ATP levels. Values were normalized to untreated cells from each donor. Significance was assessed with a one-way ANOVA using Dunnett’s multiple comparison correction (n = 3).

Article Snippet: Vorinostat , BioGems , Cat# 1497894.

Techniques: Activation Assay, Expressing, Flow Cytometry, Comparison

Journal: Cell Reports Medicine

Article Title: Pharmacological Activation of Non-canonical NF-κB Signaling Activates Latent HIV-1 Reservoirs In Vivo

doi: 10.1016/j.xcrm.2020.100037

Figure Lengend Snippet:

Article Snippet: Vorinostat , BioGems , Cat# 1497894.

Techniques: Virus, Modification, Recombinant, Viability Assay, Multiplex Assay, Digital PCR, Software, Cell Isolation

Journal: Phage (New Rochelle, N.y.)

Article Title: Isolation and Characterization of Klebsiella Phages for Phage Therapy

doi: 10.1089/phage.2020.0046

Figure Lengend Snippet: Details of Klebsiella Species and Strains Used in This Study

Article Snippet: Klebsiella michiganensis , 25444 , O1v1 , × , DSMZ Culture Collection , Toothbrush holder.

Techniques: Isolation