Journal: Inflammation

Article Title: SAA1 Promotes Ulcerative Colitis and Activating Colonic TLR4/NF-κB/NLRP3 Signaling Pathway

doi: 10.1007/s10753-025-02299-0

Figure Lengend Snippet: The proteomic analysis revealed SAA1 as a potential target for pathogenesis in UC. A Significantly expressed proteins (SEPs) were identified between the NC group and DSS group. B A heat map was used to represent the top 20 anomalous proteins in the NC group and DSS group. C Gene Ontology (GO) functional analysis was conducted on the proteins that showed changes in the NC group and DSS group. BP: Biological Process,CC: Cellular Component, MF: Molecular Function. D KEGG pathway enrichment analysis was performed on the proteins that exhibited changes in the NC group and DSS group

Article Snippet: SGA360 (HY- 122208, MedChemExpress) was dissolved in normal saline with a concentration of 0.1 mg/ml and utilized following preparation, SAA1 protein (Mouse (His); HY-P700309, MedChemExpress) was dissolved in normal saline with a concentration of 2.5 μg/ml and utilized following preparation.

Techniques: Functional Assay

Journal: Inflammation

Article Title: SAA1 Promotes Ulcerative Colitis and Activating Colonic TLR4/NF-κB/NLRP3 Signaling Pathway

doi: 10.1007/s10753-025-02299-0

Figure Lengend Snippet: Elevated LPS levels and upregulated SAA1/cytokine expression in DSS-induced UC Model. A The expression of LBP in different groups. B and C The levels of proinflammatory cytokine in different groups. (D-F) The protein expression of SAA1, TLR4, p -IKKα/β, IKKβ, p -IκBα, IκBα, p -p65, p65 and β-actin in colon was analyzed by Western blotting and grey value analysis. The results were expressed as mean ± SD, (A-C), n = 10, (D-F), n = 6. * P < 0.05 vs. NC group, ** P < 0.01 vs. NC group

Article Snippet: SGA360 (HY- 122208, MedChemExpress) was dissolved in normal saline with a concentration of 0.1 mg/ml and utilized following preparation, SAA1 protein (Mouse (His); HY-P700309, MedChemExpress) was dissolved in normal saline with a concentration of 2.5 μg/ml and utilized following preparation.

Techniques: Expressing, Western Blot

Journal: Inflammation

Article Title: SAA1 Promotes Ulcerative Colitis and Activating Colonic TLR4/NF-κB/NLRP3 Signaling Pathway

doi: 10.1007/s10753-025-02299-0

Figure Lengend Snippet: SAA1 exacerbates the severity of DSS-induced UC model. A Percentage change in body weight from baseline. B Scoring of DAI. C Representative images depicting the colon. D Measurement of colon length. E Representative images showing H&E staining of the colon. F Histological scoring based on the H&E images. G Quantification of colon calprotectin, sIgA, and IgG concentrations. H and I Protein expression analysis by Western blotting and grey value analysis for ZO- 1, occludin, claudin- 1, and β-actin in the colon. The results were expressed as mean ± SD, (A-G), n = 10, (H-I), n = 6. * P < 0.05 vs NC group, ** P < 0.01 vs NC group, # P < 0.05 vs DSS group, ## P < 0.01 vs DSS group

Article Snippet: SGA360 (HY- 122208, MedChemExpress) was dissolved in normal saline with a concentration of 0.1 mg/ml and utilized following preparation, SAA1 protein (Mouse (His); HY-P700309, MedChemExpress) was dissolved in normal saline with a concentration of 2.5 μg/ml and utilized following preparation.

Techniques: Staining, Expressing, Western Blot

Journal: Inflammation

Article Title: SAA1 Promotes Ulcerative Colitis and Activating Colonic TLR4/NF-κB/NLRP3 Signaling Pathway

doi: 10.1007/s10753-025-02299-0

Figure Lengend Snippet: SAA1 enhances the expression of proinflammatory cytokines and activates inflammatory signaling pathways. A The expression of LBP was assessed in different groups. B and C The levels of proinflammatory cytokines were measured in different groups. D and E Western blotting and grey value analysis were performed to analyze the protein expression of SAA1, TLR4, p-IKKα/β, IKKβ, p-IκBα, IκBα, p-p65, p65, and β-actin in the colon. F and G Western blot and grey value analysis were conducted to assess the protein expression of NLRP3, pro-Caspase- 1, cleaved-Caspase- 1, ASC IL- 1β, and β-actin in the colon. The results were expressed as mean ± SD, (A-C), n = 10, (D-G), n = 6. * P < 0.05 vs NC group, ** P < 0.01 vs NC group, # P < 0.05 vs DSS group, ## P < 0.01 vs DSS group

Article Snippet: SGA360 (HY- 122208, MedChemExpress) was dissolved in normal saline with a concentration of 0.1 mg/ml and utilized following preparation, SAA1 protein (Mouse (His); HY-P700309, MedChemExpress) was dissolved in normal saline with a concentration of 2.5 μg/ml and utilized following preparation.

Techniques: Expressing, Protein-Protein interactions, Western Blot

Journal: Inflammation

Article Title: SAA1 Promotes Ulcerative Colitis and Activating Colonic TLR4/NF-κB/NLRP3 Signaling Pathway

doi: 10.1007/s10753-025-02299-0

Figure Lengend Snippet: SAA1 induced an inflammatory response in intestinal epithelial cells by activating the TLR4/NF-κB signaling pathway and NLRP3 inflammasome. A The effects of different concentrations of LPS on cell proliferation inhibition and IL- 6 concentration were assessed in NCM460 cells after 12 h of exposure. The results are presented as mean ± SD. n = 5. ** p < 0.01 vs 0 μg/ml group. B and C The effect of the SAA1 inhibitor SGA360 on IL- 6 and SAA1 protein expression levels was evaluated in an LPS-induced inflammatory cell model using NCM460 cells. The results are presented as mean ± SD. n = 5. * p < 0.05 vs Control group, ** p < 0.01 vs Control group. D siRNA transfection was used to silence cellular SAA1 expression. The results are presented as mean ± SD. n = 5. * p < 0.05 vs si-Control group, ** p < 0.01 vs si-Control group. E Lentiviral transfection was employed to construct h-SAA1 stable cell lines. The results are presented as mean ± SD. n = 5. ** p < 0.01 vs h-Control group. F The effect of SAA1 on IL- 6 mRNA expression and IL- 6 content in LPS-induced NCM460 cells was investigated. The results are presented as mean ± SD. n = 5. ** p < 0.01 vs Control + LPS group. G Western blotting and gray value analysis were utilized to assess the impact of SAA1 on the expression levels of various proteins including TLR4, p-IKKα/β, IKKβ, p-IκBα, IκBα, p-p65, p65, NLRP3, Caspase- 1, ASC and IL- 1β in LPS-induced NCM460 cells. The results are presented as mean ± SD. n = 5. * p < 0.05 vs Control + LPS group, ** p < 0.01 vs Control + LPS group

Article Snippet: SGA360 (HY- 122208, MedChemExpress) was dissolved in normal saline with a concentration of 0.1 mg/ml and utilized following preparation, SAA1 protein (Mouse (His); HY-P700309, MedChemExpress) was dissolved in normal saline with a concentration of 2.5 μg/ml and utilized following preparation.

Techniques: Inhibition, Concentration Assay, Expressing, Control, Transfection, Construct, Stable Transfection, Western Blot

Journal: Inflammation

Article Title: SAA1 Promotes Ulcerative Colitis and Activating Colonic TLR4/NF-κB/NLRP3 Signaling Pathway

doi: 10.1007/s10753-025-02299-0

Figure Lengend Snippet: SAA1 promotes ulcerative colitis by directly activating the TLR4/NF-κB/NLRP3 signaling pathway. The mechanisms by which SAA1 exacerbates the progression of UC involve the activation of the TLR4/NF-κB signaling pathway, promotion of NLRP3 inflammasome activation, induction of TNF-α, IFN-γ, IL- 6 and other pro-inflammatory cytokines, as well as facilitation in inflammatory microenvironment formation. (The diagram was created with FigDraw)

Article Snippet: SGA360 (HY- 122208, MedChemExpress) was dissolved in normal saline with a concentration of 0.1 mg/ml and utilized following preparation, SAA1 protein (Mouse (His); HY-P700309, MedChemExpress) was dissolved in normal saline with a concentration of 2.5 μg/ml and utilized following preparation.

Techniques: Activation Assay

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Primer sequences used for quantitative PCR

Article Snippet: Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques:

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Radiation time response in mouse SAA1 measured using ELISA in 8 Gy irradiated female C57BL/6J mice at 0, 1, 2, 4, and 6 hours post-irradiation. n=3 per group. *, P<0.05 in the irradiated mice compared with control mice.

Article Snippet: Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Time and dose response of SAA1 after TBI. (A) SAA1 was measured using ELISA in 0, 1, 2, 4, 8 and 12 Gy irradiated female C57BL/6J mice at 0.125, 0.5, 1, 2, 3, 5 and 7 days post-irradiation. SAA1 dose-dependent change at (B) 0.25, (C) 0.5, (D) 1, (E) 2, (F) 3, (G) 5 and (H) 7 days after exposure to radiation. Each black dot represents one animal. Error bars indicate ± 1 SD for each radiation exposure group. n=8 per group (n=7 at 7 days after 8 Gy, n=2 at 7 days after 12 Gy). SAA1, serum amyloid A1; TBI, total body irradiation.

Article Snippet: Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: P values associated with two-tailed Student’s t -test for SAA1 concentrations after comparing control and irradiated groups

Article Snippet: Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: SAA1 mRNA time-dependent increase after radiation in (A) liver, (B) lung, (C) thymus, (D) spleen, (E) bone marrow and (F) small intestine measured using Quantitative PCR in control and 8 Gy irradiated female C57BL/6J mice at 0.125, 0.5, 1, 2, 3, 5 and 7 days post-irradiation. Error bars indicate ± 1 SD for each radiation exposure group. n=6 to 8 per group. *, P<0.05, *, P<0.01, ***, P<0.001, and ****, P<0.0001 in the irradiated mice compared with the control mice. SAA1, serum amyloid A1.

Article Snippet: Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Real-time Polymerase Chain Reaction, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: SAA1 expression and systemic infection parameters in the same mice. (A) SAA1 and LPS in serum and 16S rRNA in the liver of the same animals measured on 31 mice on day 0,1, 5 and 7 (7 mice on day 7, and 8 per group on day 0, 1 and 5) after exposure to 8 Gy TBI. (B) SAA1 and PCT in serum in the same animals was on 23 mice on days 0, 0.125, 0.5, 1, 2, 3, 5 and 7 (2 mice on days 7 and 3 per group on days 0, 0.125, 0.5, 1, 2, 3 and 5) after exposure to 12 Gy TBI. SAA1, serum amyloid A1; TBI, total body irradiation.

Article Snippet: Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Expressing, Infection, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Multiple linear regression analysis between serum SAA1 concentration and number of lymphocyte and irradiation dose

Article Snippet: Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: SAA1 cut-off values of classification within 2 days post-irradiation

Article Snippet: Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques:

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: PBI patterns and SAA1 response. (A) The liver of C57BL/6J mice was shielded in PBI-1 and PBI-3 and in the corresponding groups PBI-2 and PBI-4 the liver was exposed. (B) SAA1 concentration in serum and (C) mRNA expression in liver of control, total-body irradiation and PBI group at 12 hours after 8 Gy irradiation. Error bars indicate ±1 SD for each radiation exposure group. n=6 per group. **, P<0.01, ***, P<0.001, and ****, P<0.0001 in the irradiated mice compared with the control mice. PBI, partial body irradiation; SAA1, serum amyloid A1.

Article Snippet: Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Expressing, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Dynamic SAA1 concentration and corresponding date of death within 30 days after 10 Gy irradiation

Article Snippet: Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Mean and standard deviation (SD) of SAA1 concentration in 92 healthy mice

Article Snippet: Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Standard Deviation, Concentration Assay

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Kaplan-Meier survival curves of mice. P value determined by log-rank test. Serum SAA1 in mice treated with amifostine before 10 Gy irradiation and in the 10 Gy group on day −4, 1, 3, 5 and 7. n=8 in 10 Gy + Amifostine group, n=20 in 10 Gy group. ***, P<0.001. SAA1, serum amyloid A1.

Article Snippet: Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: SAA1 concentration in the Amifostine group and corresponding death date within 30 days

Article Snippet: Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Irradiation

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Clinical parameters and corresponding SAA1 concentration in all 17 NPC patients

Article Snippet: Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay

Journal: Annals of Translational Medicine

Article Title: Serum amyloid A1 as a biomarker for radiation dose estimation and lethality prediction in irradiated mouse

doi: 10.21037/atm.2019.12.27

Figure Lengend Snippet: Effect of radiotherapy on serum SAA1 in nasopharyngeal carcinoma patients. (A) The scatter plot shows SAA1 concentration before radiotherapy and corresponding expression after radiotherapy in 17 patients with nasopharyngeal carcinoma (****, P<0.0001). (B) ROC curve of SAA1 as a biomarker for predicting radiation exposure in patients with nasopharyngeal carcinoma. SAA1, serum amyloid A1.

Article Snippet: Serum proteins such as SAA1 and procalcitonin (PCT) were measured using enzyme-linked immunosorbent assay (ELISA) kits (Mouse SAA1: EK1190, Boster, Wuhan, China; Human SAA1: EK1544, Boster, Wuhan, China; PCT: E10371m, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Concentration Assay, Expressing, Biomarker Assay

Journal: Cell reports

Article Title: Toll-like receptor 2 orchestrates a tumor suppressor response in non-small cell lung cancer

doi: 10.1016/j.celrep.2022.111596

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-SAA1 , Biorbyt , Cat #orb228668; RRID:AB_2924684.

Techniques: Plasmid Preparation, Polymer, Staining, Virus, Recombinant, Sequencing, Software

Journal: The Journal of Clinical Investigation

Article Title: SAA1/FPR2 signaling between keratinocytes and neutrophils sustains chronic inflammation in Sweet syndrome

doi: 10.1172/JCI193566

Figure Lengend Snippet: ( A ) Ligand-receptor analysis reveals keratinocyte- and neutrophil-specific interactions. Keratinocytes expressed SAA1 transcripts and neutrophils expressed the FPR2 receptor (red dot, right-most column). ( B ) Dot plot demonstrating predominantly cell-specific expression of SAA1 and FPR2 transcripts. The dot size reflects the percentage of cells expressing the gene, and the color illustrates the level of gene expression. ( C ) Representative immunofluorescence staining images and quantification from 5 diseased and 5 control samples, confirming the expression of SAA1 and FPR2 in keratinocytes and neutrophils, respectively. Scale bars: 100 μm. ( D ) Dot plot comparing keratinocyte SAA1 and the control gene DEFB1 in different inflammatory skin conditions. The dot size reflects the percentage of cells expressing the gene, and the color illustrates the level of gene expression. ( E ) SAA1 secretion measured by ELISA in healthy neutrophils or healthy neutrophils exposed to keratinocytes. ( F ) Antibodies blocking SAA1 and FPR2 restored long-lived neutrophil lifespan to WT neutrophil levels. ( G ) Recombinant human SAA1 increased neutrophil survival at 72 hours ( n = 3 independent donors). Data indicate the mean ± SEM. * P < 0.05, ** P < 0.01, and *** P < 0.001, by 2-tailed, unpaired Student’s t test ( E and G ) and 1-way ANOVA with individual comparisons ( F ).

Article Snippet: For immunofluorescence microscopy, the following antibodies were used: CD3 (MCA1477, Bio-Rad), CD8 (170306S, Cell Signaling Technology), CD20 (14-0202-82, Thermo Fisher Scientific), CD68 (14-0688-82, Thermo Fisher Scientific), FCN1 (PA5-51552, Thermo Fisher Scientific), S100A8 (33254S, Cell Signaling Technology), S100A9 (34425S, Cell Signaling Technology), MHC-II (68258S, Cell Signaling Technology), IgG (66362S, Cell Signaling Technology), NE (AB68672, Abcam), ELANE (LS-B4244, LS-Bio), FPR2 (NLS1878, Novus), SAA1 (16721-1-AP, Proteintech), and MPO (sc-52707, Santa Cruz Biotechnology).

Techniques: Expressing, Gene Expression, Immunofluorescence, Staining, Control, Enzyme-linked Immunosorbent Assay, Blocking Assay, Recombinant