Journal:

Article Title: Verapamil Enhances the Antitumoral Efficacy of Oncolytic Adenoviruses

doi: 10.1038/mt.2010.22

Figure Lengend Snippet: Effect of verapamil on viral protein expression and dependence on ADP. (a) Verapamil treatment does not modify adenovirus early and late protein expression pattern. A549 cells were infected with Ad5 with or without verapamil in the extracellular medium, and expression of E1A, E3/19K, L4, and fiber proteins was analyzed at 16, 24, 40, and 48 hours postinfection. (b) Viral release kinetics of Ad5 and AdADP− in A549 cells treated with verapamil. Supernatant viral content was quantified at the indicated time points. Mean values (n = 3) ± SD are plotted. *Significant (P = 0.005 and P = 0.001 at 40 and 48 hours p.i., respectively) compared to the release of Ad5 and #significant (P = 0.03, P = 0.003, and P = 0.02 at 48, 64, and 72 hours p.i., respectively) compared to the release of AdADP−. (c) Comparative plaque size of rec700 and dl732 in the presence and absence of verapamil in A549 cells at day 6 p.i. ADP, adenovirus death protein; p.i., postinfection.

Article Snippet: Blots were probed with primary antibody anti-Ad2 E1A (clone 13 S-5; Santa Cruz Biotechnology, Santa Cruz, CA), anti-E3/19K [Tw1.3 (ref. 50 ) kindly provided by Jonathan W Yewdell, NIAID/NIH, Bethesda, MD], anti-L4/100K (clone 7/199 kindly provided by WC Russell, St Andrews University, St Andrews, UK), antiadenovirus fiber A-4 mAb (clone 4D2; Fitzgerald Industries International, Concord, MA), antihuman PARP [poly-(ADP ribose) polymerase] (clone #551024; Becton Dickinson, Erembodegem, Belgium) or anti-LC3 (Novus Biologicals, Littleton, CO).

Techniques: Expressing, Infection

Journal:

Article Title: Verapamil Enhances the Antitumoral Efficacy of Oncolytic Adenoviruses

doi: 10.1038/mt.2010.22

Figure Lengend Snippet: The selectivity of ICOVIR-5 is maintained in the presence of verapamil in an immunocompetent model in vivo. (a) Percent of body weight variation after systemic administration of PBS or ICOVIR-5 alone or combined with daily i.p. injection of 20 mg/kg of verapamil. (b) Liver E1A expression analyzed by immunohistochemistry of representative frozen liver sections of mice treated with PBS or 5 × 1010 vp of AdwtRGD, or ICOVIR-5 + daily verapamil (V) at day 5 postinfection (AdwtRGD groups were killed at day 3 due to toxicity). (c) Mean values of AST (aspartate animotransferase) and ALT (alanine aminotransferase) in serum and (d) lymphocyte and platelet concentrations in peripheral blood at day 5 postinjection (day 3 postinjection for the groups injected with AdwtRGD at 5 × 1010 vp) of the doses indicated. Mean values of five animals per group ± SD are plotted. *Significant (P < 0.05) compared to PBS and #significant compared to AdwtRGD. PBS, phosphate-buffered saline.

Article Snippet: Blots were probed with primary antibody anti-Ad2 E1A (clone 13 S-5; Santa Cruz Biotechnology, Santa Cruz, CA), anti-E3/19K [Tw1.3 (ref. 50 ) kindly provided by Jonathan W Yewdell, NIAID/NIH, Bethesda, MD], anti-L4/100K (clone 7/199 kindly provided by WC Russell, St Andrews University, St Andrews, UK), antiadenovirus fiber A-4 mAb (clone 4D2; Fitzgerald Industries International, Concord, MA), antihuman PARP [poly-(ADP ribose) polymerase] (clone #551024; Becton Dickinson, Erembodegem, Belgium) or anti-LC3 (Novus Biologicals, Littleton, CO).

Techniques: In Vivo, Injection, Expressing, Immunohistochemistry, Saline

Journal: bioRxiv

Article Title: Monoaminergic neurons share transcriptional identity across Bilaterian animals

doi: 10.1101/2025.10.10.679534

Figure Lengend Snippet: A) Linked heatmaps of transcription factor expression from pseudobulk analyses. Grey and white regions indicate orthogroups with individual homolog expression show for each species. Monoaminergic clusters shown with monoamine subtype colour coded along the top. Black boxes and labels group clusters from the same dataset. Significance of differential expression. Indicated by *. Non-pseudo-bulked datasets indicated with †. Orthogroup names shown along the right. Not all significant orthogroups shown, full figures for each species are found in supplement. B) Fluorescent HCR In Situ images showing co-expression of selected transcription factors and monoaminergic biosynthetic enzymes. Dashed boxes group images by species with co-expression against dopaminergic enzymes ( th2 , th , ple ) on the top and co-expression with serotonergic enzymes ( tph1a , tph2 , Sp-Tph , Trhn ) along the bottom. Grey schematics of the whole samples shown with known monoaminergic cell types indicated and labelled based on the literature , , , , , . S. purpuratus schematic based on Slota and McClay, 2018 . Red dashed boxes highlight the sections of the sample displayed in the fluorescent image. All enzymes coloured in red and transcription factors coloured in green. Fluorophores used are: Alexa-488, Alexa-594, Alexa-546 and DAPI (shown in supplement). Gene and probe information detailed in Table S5. C) Heatmaps of SAMap mapping scores showing alignment between bilaterian and non-bilaterian species. Non-bilaterian species shown as columns and bilaterian species shown as rows. Dashed lines follow monoaminergic rows. D) Heatmap of monoaminergic pathway and transcription factor gene expression in Nematostella vectensis . Significance of differential expression is indicated by * Cell type annotations colour coded by broad grouping. Silhouettes from Phylopic.org : silhouette images are by Christoph Schomburg ( Strongylocentrotus purpuratus ), Daniel Jaron ( Mus musculus ), Jake Warner ( Danio rerio , Nematostella vectensis ), Ramiro Morales-Hojas ( Drosophila americana as Drosophila melanogaster ), Yan Wong ( Trichoplax adhaerens ), and others ( Isodictya grandis as Spongilla lacustris ).

Article Snippet: Selected mRNA sequences were used to design HCR probes (by Molecular Instruments, Table S5).

Techniques: Expressing, Quantitative Proteomics, In Situ, Gene Expression