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  • 97
    Thermo Fisher rps26 polyclonal antibody
    Rps26 Polyclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore s26 proteasome inhibitor
    S26 Proteasome Inhibitor, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Clinical and Laboratory Standards Institute clsi document m100 s26 clinical
    Clsi Document M100 S26 Clinical, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abiocode anti phospho bad s26 antibody
    Anti Phospho Bad S26 Antibody, supplied by Abiocode, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Santa Cruz Biotechnology abortus strain s26 3
    Immunoblot analysis of C . abortus EB preparations. EBs prepared from C . abortus strains <t>S26/3</t> (lane 1), LLG (lane 2) and POS (lane 3) were run on 4–12% x NuPAGE Bis-Tris polyacrylamide gels under reducing conditions and immunoblotted with pooled post-abortion sheep serum from ewes infected with C . abortus strains S26/3 (A), LLG (B) and POS (C) as detailed in Materials and Methods. Molecular masses (kDa) are as indicated.
    Abortus Strain S26 3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Abcam anti hepatitis b virus pre s2 antigen antibody s26
    Immunoblot analysis of C . abortus EB preparations. EBs prepared from C . abortus strains <t>S26/3</t> (lane 1), LLG (lane 2) and POS (lane 3) were run on 4–12% x NuPAGE Bis-Tris polyacrylamide gels under reducing conditions and immunoblotted with pooled post-abortion sheep serum from ewes infected with C . abortus strains S26/3 (A), LLG (B) and POS (C) as detailed in Materials and Methods. Molecular masses (kDa) are as indicated.
    Anti Hepatitis B Virus Pre S2 Antigen Antibody S26, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Boeckel + Co boeco s 26 spectrometer
    Immunoblot analysis of C . abortus EB preparations. EBs prepared from C . abortus strains <t>S26/3</t> (lane 1), LLG (lane 2) and POS (lane 3) were run on 4–12% x NuPAGE Bis-Tris polyacrylamide gels under reducing conditions and immunoblotted with pooled post-abortion sheep serum from ewes infected with C . abortus strains S26/3 (A), LLG (B) and POS (C) as detailed in Materials and Methods. Molecular masses (kDa) are as indicated.
    Boeco S 26 Spectrometer, supplied by Boeckel + Co, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Panasonic Healthcare sdr s26 video camera
    Immunoblot analysis of C . abortus EB preparations. EBs prepared from C . abortus strains <t>S26/3</t> (lane 1), LLG (lane 2) and POS (lane 3) were run on 4–12% x NuPAGE Bis-Tris polyacrylamide gels under reducing conditions and immunoblotted with pooled post-abortion sheep serum from ewes infected with C . abortus strains S26/3 (A), LLG (B) and POS (C) as detailed in Materials and Methods. Molecular masses (kDa) are as indicated.
    Sdr S26 Video Camera, supplied by Panasonic Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Avanti Polar trihydroxycholest 5 en 25 r s 26 oic acid ca5 3 β 7 α 25 triol
    Intermediates in bile acid biosynthesis pathways. (A) Formation of dihydroxy-3-oxocholest-4-en-26-oic acids from cholesterol via the acidic, neutral and 25-hydroxylase pathways [ 1 , 2 ]. In the acidic and neutral pathway stereochemistry at C-25 is assumed to be 25 R unless otherwise indicated. (B) Formation of (25 R)CA 4 -7α,24 S-diol-3-one and (25 S)CA 4 -7α,24 S-diol-3-one via the 24 S-hydroxylase pathway (red box) [ 21 ] and of 25 S- and 25 R-epimers of CA 4 -7α,12α-diol-3-one in the absence of CYP27A1 [ 26 ] (gold box). For simplicity the formation and hydrolysis of CoA-thioesters in the steps preceding and succeeding C-25 racemisation are not shown. (C) Latter steps of the acidic pathway proceeding mostly in the peroxisome leading to the four diastereomers of CA 4 -7α,24-diol-3-one. In liver the dominant product is (25 R)CA 4 -7α,24 R-diol-3-one. The inset shows the formation of (25 S)CA 4 -7α,24 R-diol-3-one from its CoA-thioester following C-25 racemisation. In CTX the enzyme CYP27A1 is deficient, while in SPG5 CYP7B1 is deficient. Abbreviations: ACOX2: acyl-coenzyme A oxidase 2. AMACR: α-methylacyl-CoA racemase. BACS: bile acid-CoA synthetase. C: Cholestane. CA: Cholestanoic acid. CH25H: Cholesterol 25-hydroxylase. CYP: Cytochrome P450. DBP: D-Bifunctional Protein. HSD3B7: <t>3β-Hydroxysteroid</t> dehydrogenase type 7. LBP: L-Bifunctional protein. SPCx: Sterol carrier protein x. VLCS: Very long chain Co-A synthetase. The dihydroxy-3-oxocholest-4-en-26-oic acids CA 4 -7α,25-diol-3-one, CA 4 -7α,12α-diol-3-one and CA 4 -7α,24-diol-3-one are colour coded green, gold and red, respectively.
    Trihydroxycholest 5 En 25 R S 26 Oic Acid Ca5 3 β 7 α 25 Triol, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Moredun chlamydia abortus strain s26 3
    Intermediates in bile acid biosynthesis pathways. (A) Formation of dihydroxy-3-oxocholest-4-en-26-oic acids from cholesterol via the acidic, neutral and 25-hydroxylase pathways [ 1 , 2 ]. In the acidic and neutral pathway stereochemistry at C-25 is assumed to be 25 R unless otherwise indicated. (B) Formation of (25 R)CA 4 -7α,24 S-diol-3-one and (25 S)CA 4 -7α,24 S-diol-3-one via the 24 S-hydroxylase pathway (red box) [ 21 ] and of 25 S- and 25 R-epimers of CA 4 -7α,12α-diol-3-one in the absence of CYP27A1 [ 26 ] (gold box). For simplicity the formation and hydrolysis of CoA-thioesters in the steps preceding and succeeding C-25 racemisation are not shown. (C) Latter steps of the acidic pathway proceeding mostly in the peroxisome leading to the four diastereomers of CA 4 -7α,24-diol-3-one. In liver the dominant product is (25 R)CA 4 -7α,24 R-diol-3-one. The inset shows the formation of (25 S)CA 4 -7α,24 R-diol-3-one from its CoA-thioester following C-25 racemisation. In CTX the enzyme CYP27A1 is deficient, while in SPG5 CYP7B1 is deficient. Abbreviations: ACOX2: acyl-coenzyme A oxidase 2. AMACR: α-methylacyl-CoA racemase. BACS: bile acid-CoA synthetase. C: Cholestane. CA: Cholestanoic acid. CH25H: Cholesterol 25-hydroxylase. CYP: Cytochrome P450. DBP: D-Bifunctional Protein. HSD3B7: <t>3β-Hydroxysteroid</t> dehydrogenase type 7. LBP: L-Bifunctional protein. SPCx: Sterol carrier protein x. VLCS: Very long chain Co-A synthetase. The dihydroxy-3-oxocholest-4-en-26-oic acids CA 4 -7α,25-diol-3-one, CA 4 -7α,12α-diol-3-one and CA 4 -7α,24-diol-3-one are colour coded green, gold and red, respectively.
    Chlamydia Abortus Strain S26 3, supplied by Moredun, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Clinical and Laboratory Standards Institute clsi m100 s26 clinical
    Intermediates in bile acid biosynthesis pathways. (A) Formation of dihydroxy-3-oxocholest-4-en-26-oic acids from cholesterol via the acidic, neutral and 25-hydroxylase pathways [ 1 , 2 ]. In the acidic and neutral pathway stereochemistry at C-25 is assumed to be 25 R unless otherwise indicated. (B) Formation of (25 R)CA 4 -7α,24 S-diol-3-one and (25 S)CA 4 -7α,24 S-diol-3-one via the 24 S-hydroxylase pathway (red box) [ 21 ] and of 25 S- and 25 R-epimers of CA 4 -7α,12α-diol-3-one in the absence of CYP27A1 [ 26 ] (gold box). For simplicity the formation and hydrolysis of CoA-thioesters in the steps preceding and succeeding C-25 racemisation are not shown. (C) Latter steps of the acidic pathway proceeding mostly in the peroxisome leading to the four diastereomers of CA 4 -7α,24-diol-3-one. In liver the dominant product is (25 R)CA 4 -7α,24 R-diol-3-one. The inset shows the formation of (25 S)CA 4 -7α,24 R-diol-3-one from its CoA-thioester following C-25 racemisation. In CTX the enzyme CYP27A1 is deficient, while in SPG5 CYP7B1 is deficient. Abbreviations: ACOX2: acyl-coenzyme A oxidase 2. AMACR: α-methylacyl-CoA racemase. BACS: bile acid-CoA synthetase. C: Cholestane. CA: Cholestanoic acid. CH25H: Cholesterol 25-hydroxylase. CYP: Cytochrome P450. DBP: D-Bifunctional Protein. HSD3B7: <t>3β-Hydroxysteroid</t> dehydrogenase type 7. LBP: L-Bifunctional protein. SPCx: Sterol carrier protein x. VLCS: Very long chain Co-A synthetase. The dihydroxy-3-oxocholest-4-en-26-oic acids CA 4 -7α,25-diol-3-one, CA 4 -7α,12α-diol-3-one and CA 4 -7α,24-diol-3-one are colour coded green, gold and red, respectively.
    Clsi M100 S26 Clinical, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    DSMZ comc chlamydia abortus strain s26 3
    C . abortus <t>S26/3</t> <t>COMC</t> proteins resolved by SDS-PAGE. The 26 x 2.5 mm deep gel slices excised are indicated.
    Comc Chlamydia Abortus Strain S26 3, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Abcam mouse anti hepatitis b virus pres2 antigen antibody s26
    C . abortus <t>S26/3</t> <t>COMC</t> proteins resolved by SDS-PAGE. The 26 x 2.5 mm deep gel slices excised are indicated.
    Mouse Anti Hepatitis B Virus Pres2 Antigen Antibody S26, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Clinical and Laboratory Standards Institute guideline document m100 s26
    C . abortus <t>S26/3</t> <t>COMC</t> proteins resolved by SDS-PAGE. The 26 x 2.5 mm deep gel slices excised are indicated.
    Guideline Document M100 S26, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Moredun inoculum chlamydia abortus strain s26 3
    C . abortus <t>S26/3</t> <t>COMC</t> proteins resolved by SDS-PAGE. The 26 x 2.5 mm deep gel slices excised are indicated.
    Inoculum Chlamydia Abortus Strain S26 3, supplied by Moredun, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Panasonic Healthcare panasonic sdr s26 camcorder
    C . abortus <t>S26/3</t> <t>COMC</t> proteins resolved by SDS-PAGE. The 26 x 2.5 mm deep gel slices excised are indicated.
    Panasonic Sdr S26 Camcorder, supplied by Panasonic Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Hoefer s26 3 infected total cell lysate
    C . abortus <t>S26/3</t> <t>COMC</t> proteins resolved by SDS-PAGE. The 26 x 2.5 mm deep gel slices excised are indicated.
    S26 3 Infected Total Cell Lysate, supplied by Hoefer, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MDPI s26 s47
    C . abortus <t>S26/3</t> <t>COMC</t> proteins resolved by SDS-PAGE. The 26 x 2.5 mm deep gel slices excised are indicated.
    S26 S47, supplied by MDPI, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Johnson & Johnson s26 s48 you j j
    C . abortus <t>S26/3</t> <t>COMC</t> proteins resolved by SDS-PAGE. The 26 x 2.5 mm deep gel slices excised are indicated.
    S26 S48 You J J, supplied by Johnson & Johnson, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Clinical and Laboratory Standards Institute s26
    C . abortus <t>S26/3</t> <t>COMC</t> proteins resolved by SDS-PAGE. The 26 x 2.5 mm deep gel slices excised are indicated.
    S26, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Wyeth Biopharma wyeth s 26 sma pdf gold
    C . abortus <t>S26/3</t> <t>COMC</t> proteins resolved by SDS-PAGE. The 26 x 2.5 mm deep gel slices excised are indicated.
    Wyeth S 26 Sma Pdf Gold, supplied by Wyeth Biopharma, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Immunoblot analysis of C . abortus EB preparations. EBs prepared from C . abortus strains S26/3 (lane 1), LLG (lane 2) and POS (lane 3) were run on 4–12% x NuPAGE Bis-Tris polyacrylamide gels under reducing conditions and immunoblotted with pooled post-abortion sheep serum from ewes infected with C . abortus strains S26/3 (A), LLG (B) and POS (C) as detailed in Materials and Methods. Molecular masses (kDa) are as indicated.

    Journal: PLoS ONE

    Article Title: Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS

    doi: 10.1371/journal.pone.0177653

    Figure Lengend Snippet: Immunoblot analysis of C . abortus EB preparations. EBs prepared from C . abortus strains S26/3 (lane 1), LLG (lane 2) and POS (lane 3) were run on 4–12% x NuPAGE Bis-Tris polyacrylamide gels under reducing conditions and immunoblotted with pooled post-abortion sheep serum from ewes infected with C . abortus strains S26/3 (A), LLG (B) and POS (C) as detailed in Materials and Methods. Molecular masses (kDa) are as indicated.

    Article Snippet: After quenching of endogenous peroxidase activity with 3% hydrogen peroxide (Sigma) in methanol (v/v), the sections were washed in TBS, non-specific antibody binding blocked by incubation in 20% normal goat serum (NGS) in TBS for 30 min prior to overnight incubation at 4°C with a mouse monoclonal antibody (mAb) raised against the lipopolysaccharide (LPS) of C . abortus strain S26/3 (mAb 13/4, Santa Cruz Biotechnology, Heidelberg, Germany) diluted in 10% NGS/ TBS [ ].

    Techniques: Infection

    Histopathological changes in the placentas of sheep infected with C . abortus strains. (A) S26/3, note large areas of infiltration by large numbers of leucocytes into the epithelium of the cotyledon forming large amounts of necro-suppurative material (arrows); (B) S26/3, the leucocytes were mixed, primarily neutrophilic, many degenerate, inflammatory cells; (C) S26/3, arteritis as denoted by infiltration of medium to large numbers of leucocytes into the blood vessel wall, which were (D) neutrophils and fewer monocytes; (E) S26/3, severe fibrinoid necrosis as denoted by a dense band of intensely eosinophilic material within the arterial wall (arrows); (F) S26/3, early thrombosis of blood vessels. Note large thrombus comprised of palisading layers of degenerate erythrocytes, leukocytes and proteinacious material along with loss of definition of the tunica intima (arrows). (G) LLG, note less extensive infiltration of leucocytes (arrows) with less penetration into the deeper tissue compared to S26/3. (H) POS, note less extensive infiltration of leucocytes (arrows), especially the deeper tissues beneath the epithelium, compared to both S23/6 and LLG, the lesions were more associated with the intercotyledonary membrane than cotyledonary villi but of a similar morphology being comprised of mixed leucocytes but primarily neutrophils (I). (J) Negative control, note absence of inflammatory cells and necrotic material.

    Journal: PLoS ONE

    Article Title: Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS

    doi: 10.1371/journal.pone.0177653

    Figure Lengend Snippet: Histopathological changes in the placentas of sheep infected with C . abortus strains. (A) S26/3, note large areas of infiltration by large numbers of leucocytes into the epithelium of the cotyledon forming large amounts of necro-suppurative material (arrows); (B) S26/3, the leucocytes were mixed, primarily neutrophilic, many degenerate, inflammatory cells; (C) S26/3, arteritis as denoted by infiltration of medium to large numbers of leucocytes into the blood vessel wall, which were (D) neutrophils and fewer monocytes; (E) S26/3, severe fibrinoid necrosis as denoted by a dense band of intensely eosinophilic material within the arterial wall (arrows); (F) S26/3, early thrombosis of blood vessels. Note large thrombus comprised of palisading layers of degenerate erythrocytes, leukocytes and proteinacious material along with loss of definition of the tunica intima (arrows). (G) LLG, note less extensive infiltration of leucocytes (arrows) with less penetration into the deeper tissue compared to S26/3. (H) POS, note less extensive infiltration of leucocytes (arrows), especially the deeper tissues beneath the epithelium, compared to both S23/6 and LLG, the lesions were more associated with the intercotyledonary membrane than cotyledonary villi but of a similar morphology being comprised of mixed leucocytes but primarily neutrophils (I). (J) Negative control, note absence of inflammatory cells and necrotic material.

    Article Snippet: After quenching of endogenous peroxidase activity with 3% hydrogen peroxide (Sigma) in methanol (v/v), the sections were washed in TBS, non-specific antibody binding blocked by incubation in 20% normal goat serum (NGS) in TBS for 30 min prior to overnight incubation at 4°C with a mouse monoclonal antibody (mAb) raised against the lipopolysaccharide (LPS) of C . abortus strain S26/3 (mAb 13/4, Santa Cruz Biotechnology, Heidelberg, Germany) diluted in 10% NGS/ TBS [ ].

    Techniques: Infection, Negative Control

    Serological responses to C . abortus strains. Detection of C . abortus antibody in ewes that aborted (includes non-viable births) (-—-) and lambed (-. -) following challenge with C . abortus strains S26/3 (A), LLG (B) and POS (C) or with negative control inoculum (D). Means (± SEM) of normalised responses (see Materials and Methods ) are shown. 100% is equivalent to an OD450nm of 2.25. The lambing/abortion period is indicated by the horizontal double-headed arrows.

    Journal: PLoS ONE

    Article Title: Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS

    doi: 10.1371/journal.pone.0177653

    Figure Lengend Snippet: Serological responses to C . abortus strains. Detection of C . abortus antibody in ewes that aborted (includes non-viable births) (-—-) and lambed (-. -) following challenge with C . abortus strains S26/3 (A), LLG (B) and POS (C) or with negative control inoculum (D). Means (± SEM) of normalised responses (see Materials and Methods ) are shown. 100% is equivalent to an OD450nm of 2.25. The lambing/abortion period is indicated by the horizontal double-headed arrows.

    Article Snippet: After quenching of endogenous peroxidase activity with 3% hydrogen peroxide (Sigma) in methanol (v/v), the sections were washed in TBS, non-specific antibody binding blocked by incubation in 20% normal goat serum (NGS) in TBS for 30 min prior to overnight incubation at 4°C with a mouse monoclonal antibody (mAb) raised against the lipopolysaccharide (LPS) of C . abortus strain S26/3 (mAb 13/4, Santa Cruz Biotechnology, Heidelberg, Germany) diluted in 10% NGS/ TBS [ ].

    Techniques: Negative Control

    Immunohistochemical detection of chlamydial antigen in placentas. Sheep placentas infected with C. abortus strains S26/3 (A and B), LLG (C and D), POS (E) and negative control (F) and c ounterstained with haematoxylin. (A) S26/3, note extensive positive labelling and (B) that this was most intense and abundant in the trophoblast layer, both still attached and sloughed, and the immediately surrounding cells. (C) LLG, note positive labeling restricted to the epithelium (trophoblast) layer of the cotyledon, primarily at the bases of the cotyledonary villi and (D) in occasional foci in the intercotyledonary membrane. (E) POS, note positive labeling restricted to the epithelium (trophoblast) layer and immediately adjacent cells of the placenta in a multifocal distribution. Intense inflammatory cell infiltration, primarily neutrophils, was also present but not usually associated with the immunolabelling (arrows). (F) Negative control, note total absence of any immunolabelling and no significant infiltration by inflammatory cells.

    Journal: PLoS ONE

    Article Title: Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS

    doi: 10.1371/journal.pone.0177653

    Figure Lengend Snippet: Immunohistochemical detection of chlamydial antigen in placentas. Sheep placentas infected with C. abortus strains S26/3 (A and B), LLG (C and D), POS (E) and negative control (F) and c ounterstained with haematoxylin. (A) S26/3, note extensive positive labelling and (B) that this was most intense and abundant in the trophoblast layer, both still attached and sloughed, and the immediately surrounding cells. (C) LLG, note positive labeling restricted to the epithelium (trophoblast) layer of the cotyledon, primarily at the bases of the cotyledonary villi and (D) in occasional foci in the intercotyledonary membrane. (E) POS, note positive labeling restricted to the epithelium (trophoblast) layer and immediately adjacent cells of the placenta in a multifocal distribution. Intense inflammatory cell infiltration, primarily neutrophils, was also present but not usually associated with the immunolabelling (arrows). (F) Negative control, note total absence of any immunolabelling and no significant infiltration by inflammatory cells.

    Article Snippet: After quenching of endogenous peroxidase activity with 3% hydrogen peroxide (Sigma) in methanol (v/v), the sections were washed in TBS, non-specific antibody binding blocked by incubation in 20% normal goat serum (NGS) in TBS for 30 min prior to overnight incubation at 4°C with a mouse monoclonal antibody (mAb) raised against the lipopolysaccharide (LPS) of C . abortus strain S26/3 (mAb 13/4, Santa Cruz Biotechnology, Heidelberg, Germany) diluted in 10% NGS/ TBS [ ].

    Techniques: Immunohistochemistry, Infection, Negative Control, Labeling

    Intermediates in bile acid biosynthesis pathways. (A) Formation of dihydroxy-3-oxocholest-4-en-26-oic acids from cholesterol via the acidic, neutral and 25-hydroxylase pathways [ 1 , 2 ]. In the acidic and neutral pathway stereochemistry at C-25 is assumed to be 25 R unless otherwise indicated. (B) Formation of (25 R)CA 4 -7α,24 S-diol-3-one and (25 S)CA 4 -7α,24 S-diol-3-one via the 24 S-hydroxylase pathway (red box) [ 21 ] and of 25 S- and 25 R-epimers of CA 4 -7α,12α-diol-3-one in the absence of CYP27A1 [ 26 ] (gold box). For simplicity the formation and hydrolysis of CoA-thioesters in the steps preceding and succeeding C-25 racemisation are not shown. (C) Latter steps of the acidic pathway proceeding mostly in the peroxisome leading to the four diastereomers of CA 4 -7α,24-diol-3-one. In liver the dominant product is (25 R)CA 4 -7α,24 R-diol-3-one. The inset shows the formation of (25 S)CA 4 -7α,24 R-diol-3-one from its CoA-thioester following C-25 racemisation. In CTX the enzyme CYP27A1 is deficient, while in SPG5 CYP7B1 is deficient. Abbreviations: ACOX2: acyl-coenzyme A oxidase 2. AMACR: α-methylacyl-CoA racemase. BACS: bile acid-CoA synthetase. C: Cholestane. CA: Cholestanoic acid. CH25H: Cholesterol 25-hydroxylase. CYP: Cytochrome P450. DBP: D-Bifunctional Protein. HSD3B7: 3β-Hydroxysteroid dehydrogenase type 7. LBP: L-Bifunctional protein. SPCx: Sterol carrier protein x. VLCS: Very long chain Co-A synthetase. The dihydroxy-3-oxocholest-4-en-26-oic acids CA 4 -7α,25-diol-3-one, CA 4 -7α,12α-diol-3-one and CA 4 -7α,24-diol-3-one are colour coded green, gold and red, respectively.

    Journal: Biochimie

    Article Title: Identification of 7α,24-dihydroxy-3-oxocholest-4-en-26-oic and 7α,25-dihydroxy-3-oxocholest-4-en-26-oic acids in human cerebrospinal fluid and plasma

    doi: 10.1016/j.biochi.2018.06.020

    Figure Lengend Snippet: Intermediates in bile acid biosynthesis pathways. (A) Formation of dihydroxy-3-oxocholest-4-en-26-oic acids from cholesterol via the acidic, neutral and 25-hydroxylase pathways [ 1 , 2 ]. In the acidic and neutral pathway stereochemistry at C-25 is assumed to be 25 R unless otherwise indicated. (B) Formation of (25 R)CA 4 -7α,24 S-diol-3-one and (25 S)CA 4 -7α,24 S-diol-3-one via the 24 S-hydroxylase pathway (red box) [ 21 ] and of 25 S- and 25 R-epimers of CA 4 -7α,12α-diol-3-one in the absence of CYP27A1 [ 26 ] (gold box). For simplicity the formation and hydrolysis of CoA-thioesters in the steps preceding and succeeding C-25 racemisation are not shown. (C) Latter steps of the acidic pathway proceeding mostly in the peroxisome leading to the four diastereomers of CA 4 -7α,24-diol-3-one. In liver the dominant product is (25 R)CA 4 -7α,24 R-diol-3-one. The inset shows the formation of (25 S)CA 4 -7α,24 R-diol-3-one from its CoA-thioester following C-25 racemisation. In CTX the enzyme CYP27A1 is deficient, while in SPG5 CYP7B1 is deficient. Abbreviations: ACOX2: acyl-coenzyme A oxidase 2. AMACR: α-methylacyl-CoA racemase. BACS: bile acid-CoA synthetase. C: Cholestane. CA: Cholestanoic acid. CH25H: Cholesterol 25-hydroxylase. CYP: Cytochrome P450. DBP: D-Bifunctional Protein. HSD3B7: 3β-Hydroxysteroid dehydrogenase type 7. LBP: L-Bifunctional protein. SPCx: Sterol carrier protein x. VLCS: Very long chain Co-A synthetase. The dihydroxy-3-oxocholest-4-en-26-oic acids CA 4 -7α,25-diol-3-one, CA 4 -7α,12α-diol-3-one and CA 4 -7α,24-diol-3-one are colour coded green, gold and red, respectively.

    Article Snippet: The authentic standards of 3β,7α,24 S-trihydroxycholest-5-en-(25 R)26-oic acid ((25 R)CA5 -3β,7α,24 S-triol) and 3β,7α,25-trihydroxycholest-5-en-(25 R/S)26-oic acid (CA5 -3β,7α,25-triol) were from Avanti Polar Lipids Inc (Alabaster, AL).

    Techniques:

    Conversion of 3β-hydroxy-5-ene acids to their 3-oxo-4-ene analogues by cholesterol oxidase enzyme from Streptomyces Sp and derivatisation with [ 2 H 5 ]GP to give syn and anti conformers. In CA 5 -3β,7α,24 S-triol and CA 4 -7α,24 S-diol-3-one R 1 = OH and R 2 = H. In CA 5 -3β,7α,25-triol and CA 4 -7α,25-diol-3-one R 1 = H and R 2 = OH (upper panel). Structures of (25 S)CA 4 -7α,25-diol-3-one and its isomers (25 R)CA 4 -7α,25-diol-3-one, (25 R)CA 4 -7α,24-diol-3-one and (25 R)CA 4 -7α,12α-diol-3-one are shown in the lower panel.

    Journal: Biochimie

    Article Title: Identification of 7α,24-dihydroxy-3-oxocholest-4-en-26-oic and 7α,25-dihydroxy-3-oxocholest-4-en-26-oic acids in human cerebrospinal fluid and plasma

    doi: 10.1016/j.biochi.2018.06.020

    Figure Lengend Snippet: Conversion of 3β-hydroxy-5-ene acids to their 3-oxo-4-ene analogues by cholesterol oxidase enzyme from Streptomyces Sp and derivatisation with [ 2 H 5 ]GP to give syn and anti conformers. In CA 5 -3β,7α,24 S-triol and CA 4 -7α,24 S-diol-3-one R 1 = OH and R 2 = H. In CA 5 -3β,7α,25-triol and CA 4 -7α,25-diol-3-one R 1 = H and R 2 = OH (upper panel). Structures of (25 S)CA 4 -7α,25-diol-3-one and its isomers (25 R)CA 4 -7α,25-diol-3-one, (25 R)CA 4 -7α,24-diol-3-one and (25 R)CA 4 -7α,12α-diol-3-one are shown in the lower panel.

    Article Snippet: The authentic standards of 3β,7α,24 S-trihydroxycholest-5-en-(25 R)26-oic acid ((25 R)CA5 -3β,7α,24 S-triol) and 3β,7α,25-trihydroxycholest-5-en-(25 R/S)26-oic acid (CA5 -3β,7α,25-triol) were from Avanti Polar Lipids Inc (Alabaster, AL).

    Techniques:

    C . abortus S26/3 COMC proteins resolved by SDS-PAGE. The 26 x 2.5 mm deep gel slices excised are indicated.

    Journal: PLoS ONE

    Article Title: Proteomic characterisation of the Chlamydia abortus outer membrane complex (COMC) using combined rapid monolithic column liquid chromatography and fast MS/MS scanning

    doi: 10.1371/journal.pone.0224070

    Figure Lengend Snippet: C . abortus S26/3 COMC proteins resolved by SDS-PAGE. The 26 x 2.5 mm deep gel slices excised are indicated.

    Article Snippet: Propagation of C . abortus and preparation of the COMC Chlamydia abortus strain S26/3 (available from DSMZ, product number DSM 27085), isolated at Moredun Research Institute in Scotland in 1979 from a vaccinated ewe that aborted [ ], was propagated in McCoy cells (obtained from ECACC General Collection in 2005, product number 90010305) in accordance with previously published protocols [ ].

    Techniques: SDS Page