Journal: Frontiers in Aging Neuroscience

Article Title: Astrocytic Ror2-induced imbalance in brain and gut homeostasis contributes to chronic post-thoracotomy pain

doi: 10.3389/fnagi.2025.1675725

Figure Lengend Snippet: CPTP primarily drove the transition of astrocytes in the ACC to the A1 phenotype. (A,C,E) Western blotting of GFAP, C3, and S100A10 levels in the CPTP and sham groups across POD 7, 14, and 21. (B,D,F) Western blotting of GFAP, C3, and S100A10 levels in all groups on POD 21. (G,I) Quantitative analysis of C3 or S100A10 co-localization with GFAP on POD 7, 14, and 21. (H,J) Immunofluorescence analysis revealed C3 or S100A10 (all red) co-localization with GFAP (green) on POD 7, 14, and 21. Scale bar: 50 μm. ### P < 0.001 compared with the non-CPTP group; * P < 0.05, ** P < 0.01, and *** P < 0.001 compared with the sham group; one-way ANOVA; n = 3 per group.

Article Snippet: S100A10 , Mouse , Cell signaling technology , 5529 , 1:1000.

Techniques: Western Blot, Immunofluorescence

Journal: Frontiers in Aging Neuroscience

Article Title: Astrocytic Ror2-induced imbalance in brain and gut homeostasis contributes to chronic post-thoracotomy pain

doi: 10.3389/fnagi.2025.1675725

Figure Lengend Snippet: Astrocytic Ror2 knockdown rebalanced the A1/A2 ratio in the ACC in CPTP rats. (A,B) Western blotting analysis of C3 and S100A10 after spinal delivery of AAV2/9-GFAP-miR30-shRor2 or AAV2/9-GFAP-Control in CPTP rats. (C,D) Quantitative co-localization analysis of C3 or S100A10 with GFAP in the AAV2/9-GFAP-miR30-shRor2-CPTP group compared with the AAV2/9-GFAP-Control-CPTP group (POD 21), n = 3 per group. ** P < 0.01, and *** P < 0.001 compared with the AAV2/9-GFAP-Control-CPTP group; one-way ANOVA; n = 3 per group. (E,F) Immunofluorescence staining revealed co-localization of C3 or S100A10 (all red) with GFAP (green) in the AAV2/9-GFAP-miR30-shRor2-CPTP group on POD 7, 14, and 21 compared with the AAV2/9-GFAP-Control-CPTP group on POD 21; n = 3 per group.

Article Snippet: S100A10 , Mouse , Cell signaling technology , 5529 , 1:1000.

Techniques: Knockdown, Western Blot, Control, Immunofluorescence, Staining

Journal: bioRxiv

Article Title: Molecular architecture of the tumor microenvironment caused by BRCA1 and BRCA2 somatic mutations in lung adenocarcinoma

doi: 10.64898/2026.01.29.702641

Figure Lengend Snippet: Targeting BRCA1 mutation-related risk genes inhibits tumor growth. A-B. Survival analysis of BRCA1 -mutant signature score in TCGA-LUAD (A) and OAK (B) cohorts. C. Survival analysis of S100A10 (left) and LDHA (right) in TCGA-LUAD cohort according to the expression median. D. The effects of sh- LDHA , S100A10 , GAPDH , and vector on cell proliferation were determined by a cell proliferation assay in A549 LUAD cell line. E. The effects of sh- LDHA , S100A10 , and GAPDH on protein levels in A549 cell line (The original image is shown in Fig. S12A). F. The molecular perturbations of 4-R genes in A549 cell line at 10 µM concentration (24 h) from the LINCS data resource. NCS: Normalized connectivity score. G. The effects of HDAC inhibitors concentration on cell proliferation in A549 cell line. H. Cell replication levels after treatment with different concentrations of entinostat (left) and vorinostat (right) in LUAD cell lines. I. The protein levels of LDHA , S100A10 , and GAPDH after treatment with vorinostat (left) and belinostat (right) in A549 cell line were measured using western blotting (The original image is shown in Fig. S12B). J. The mRNA levels of LDHA and GAPDH after treatment (3 µM) with vorinostat (left) and belinostat (right) in A549 cell line were measured using qPCR. Significance levels were determined by Student’s t-test. For all statistical tests, *P≤0.05; **P≤0.01; ***P≤0.001.

Article Snippet: The following antibodies were used: GAPDH Rabbit mAb (#A19056, 1:100,000; Abclonal), S100A10 Rabbit mAb (#A13634, 1:1000; Abclonal), LDHA Rabbit mAb (#A0861, 1:1000; Abclonal), β-Tubulin Rabbit mAb (#A12289, 1:10000; Abclonal) and HRP Goat anti-Rabbit IgG(H+L) (#111-035-003, 1:10000; Jackson).

Techniques: Mutagenesis, Expressing, Plasmid Preparation, Proliferation Assay, Concentration Assay, Western Blot

Journal: European Journal of Medical Research

Article Title: Peripheral Blood-Derived Mesenchymal Stem Cells Promote A2 Phenotype Polarization in Astrocytes via TGF-β-Mediated PI3K/Akt Pathway Activation

doi: 10.1186/s40001-025-02814-x

Figure Lengend Snippet: Effect of PB-MSCs on A2 phenotype polarization of AS via the TGF-β/PI3K/Akt pathway (mean ± SD, n = 3, *P < 0.05) A IF assay for S100A10 expression in each culture group (scale = 100 μm); B Average fluorescence intensity of S100A10 in each culture group

Article Snippet: Subsequently, samples were incubated overnight at 4°C with rabbit anti-rat S100A10 primary antibody (1:1000; Cat# A1987, ABclonal Technology, Wuhan, China).

Techniques: Expressing, Fluorescence

Journal: European Journal of Medical Research

Article Title: Peripheral Blood-Derived Mesenchymal Stem Cells Promote A2 Phenotype Polarization in Astrocytes via TGF-β-Mediated PI3K/Akt Pathway Activation

doi: 10.1186/s40001-025-02814-x

Figure Lengend Snippet: Effect of recombinant TGF-β on AS phenotype as well as Akt phosphorylation (mean ± SD, n = 3 , * P < 0.05) A IF detection of S100A10 expression (scale = 100 μm); B Average fluorescence intensity results of S100A10 C WB results of p-Akt, Akt and GAPDH; D Phosphorylation of Akt

Article Snippet: Subsequently, samples were incubated overnight at 4°C with rabbit anti-rat S100A10 primary antibody (1:1000; Cat# A1987, ABclonal Technology, Wuhan, China).

Techniques: Recombinant, Phospho-proteomics, Expressing, Fluorescence

Journal: The Journal of International Medical Research

Article Title: Clinical significance of serum S100A10 in lung cancer

doi: 10.1177/03000605211049653

Figure Lengend Snippet: Demographic and clinical characteristics of patients with lung cancer ( n = 82) that were included in this prospective study of the clinical significance of serum S100 calcium-binding protein A10 (S100A10) levels in lung cancer.

Article Snippet: Serum S100A10 levels were quantified using an enzyme-linked immunosorbent assay (ELISA) kit (CSB-EL020623HU; Wuhan CUSABIO, Hubei, China).

Techniques:

Journal: The Journal of International Medical Research

Article Title: Clinical significance of serum S100A10 in lung cancer

doi: 10.1177/03000605211049653

Figure Lengend Snippet: Levels of serum S100 calcium-binding protein A10 (S100A10) and three tumour markers in the three groups of study participants: patients with lung cancer ( n = 82), patients with benign lung nodules ( n = 21) and healthy control subjects ( n = 50). (a) S100A10; (b) carcinoembryonic antigen (CEA); (c) cytokeratin 19 fragment (Cyfra21-1); (d) squamous cell carcinoma antigen (SCC). The colour version of this figure is available at: http://imr.sagepub.com.

Article Snippet: Serum S100A10 levels were quantified using an enzyme-linked immunosorbent assay (ELISA) kit (CSB-EL020623HU; Wuhan CUSABIO, Hubei, China).

Techniques: Binding Assay, Control

Journal: The Journal of International Medical Research

Article Title: Clinical significance of serum S100A10 in lung cancer

doi: 10.1177/03000605211049653

Figure Lengend Snippet: Relationship between the levels of serum S100 calcium-binding protein A10 (S100A10) and clinical characteristics in patients with lung cancer ( n = 82): (a) Tumor–Node–Metastasis (TNM) stage; (b) lymph node metastasis; (c) histological type; (d) tumour size. The colour version of this figure is available at: http://imr.sagepub.com.

Article Snippet: Serum S100A10 levels were quantified using an enzyme-linked immunosorbent assay (ELISA) kit (CSB-EL020623HU; Wuhan CUSABIO, Hubei, China).

Techniques: Binding Assay

Journal: The Journal of International Medical Research

Article Title: Clinical significance of serum S100A10 in lung cancer

doi: 10.1177/03000605211049653

Figure Lengend Snippet: Diagnostic efficacy of serum S100 calcium-binding protein A10 (S100A10) and carcinoembryonic antigen (CEA) for lung cancer. The colour version of this figure is available at: http://imr.sagepub.com.

Article Snippet: Serum S100A10 levels were quantified using an enzyme-linked immunosorbent assay (ELISA) kit (CSB-EL020623HU; Wuhan CUSABIO, Hubei, China).

Techniques: Diagnostic Assay, Binding Assay

Journal: Frontiers in Physiology

Article Title: Annexin II Light Chain p11 Interacts With ENaC to Increase Functional Activity at the Membrane

doi: 10.3389/fphys.2019.00007

Figure Lengend Snippet: Identification of novel p11-ENaC interacting partners by LC–MS/MS analysis. (A) Schematic representation of the proteomics approach used to identify proteins interacting with the p11-ENaC complex. HEK293 cell lysates were co-transfected with p11-myc-DDK and αβ-HAγ ENaC. After 24 h expression, cells were lysed and co-immunoprecipitated sequentially with anti-myc-DDK and anti-HA with additional protein G-agarose beads in each tube. The eluent of the complex was then collected and subjected to SDS-PAGE 1D gel. The gel was carefully excised into three fragments and processed through in-gel trypsinization for LC–MS/MS analysis to determine potential proteins interacting with ENaC and p11. (B) MS spectrum recorded during analysis of purified protein complexes. Red text denotes specific peptides that identified proteins listed in . The list of identified peptides was used to tag the corresponding peptide-related ion spectra based on m/z differences, deviations from the predicted elution times, and the match between the theoretical and observed isotopic envelopes. The maximum deviation accepted in m/z and the retention time was established separately for each of the processed LC–MS spectra to account for possible variations in mass measurement accuracy and chromatographic separation between runs.

Article Snippet: For co-immunoprecipitation, HEK293 cells were co-transfected with p11-myc-DDK plasmid (RC204992, OriGene Technologies, Rockville, MD, United States) and plasmids encoding α-, β HA -, and γ-ENaC subunits.

Techniques: Liquid Chromatography with Mass Spectroscopy, Transfection, Expressing, Immunoprecipitation, SDS Page, Purification, Mass Measurement

Journal: Frontiers in Physiology

Article Title: Annexin II Light Chain p11 Interacts With ENaC to Increase Functional Activity at the Membrane

doi: 10.3389/fphys.2019.00007

Figure Lengend Snippet: p11 co-immunoprecipitates with ENaC. HEK293 cells were transfected with p11-myc-DDK and αβ HA γ ENaC. After 24 h, cells were lysed and immunoprecipitated with anti-p11 (ab89438) and protein G-agarose beads. Precipitates were analyzed by SDS-PAGE and immunoblotted (IB) with anti-HA to detect β HA ENaC (Mr ∼ 72 kDa) and p11 (Mr ∼ 11 kDa). ENaC was co-immunoprecipitated in the presence of anti-p11 but not when an unrelated antibody was used. Data shown are representative of three independent experiments with similar results.

Article Snippet: For co-immunoprecipitation, HEK293 cells were co-transfected with p11-myc-DDK plasmid (RC204992, OriGene Technologies, Rockville, MD, United States) and plasmids encoding α-, β HA -, and γ-ENaC subunits.

Techniques: Transfection, Immunoprecipitation, SDS Page