Journal: Molecular Medicine Reports

Article Title: S100A1 expression is increased in spinal cord injury and promotes inflammation, oxidative stress and apoptosis of PC12 cells induced by LPS via ERK signaling

doi: 10.3892/mmr.2022.12917

Figure Lengend Snippet: S100A1 regulates the level of inflammation in PC12 cells through the ERK signaling pathway. (A) The mRNA expression levels of inflammatory cytokines (IL-1β, IL-6 and TNF-α) and anti-inflammatory cytokines (IL-10) were detected using reverse transcription-quantitative PCR in the Control, LPS, LPS with S100A1 silencing/overexpression, LPS with S100A1 overexpression and EKR inhibitor groups. (B) The protein levels of inflammatory cytokines (IL-1β, IL-6 and TNF-α) and anti-inflammatory cytokines (IL-10) were detected using ELISA in the Control, LPS, LPS with S100A1 silencing/overexpression, LPS with S100A1 overexpression and EKR inhibitor groups. n=3. *P<0.05, **P<0.01, ***P<0.001 vs. Control group. # P<0.05, ## P<0.01, ### P<0.001 vs. LPS group. & P<0.05, && P<0.01 vs. LPS + si-S100A1 group. $ P<0.05, $$ P<0.01 vs. LPS + ov-S100A1 group. LPS, lipopolysaccharide; ov, overexpression; si, short interfering.

Article Snippet: In addition, the cells transfected with ov-S100A1 plasmid were treated with the ERK inhibitor, MK-8353 (100 nM, MedChemExpress), 12 h prior to exposure to LPS, as previously described ( ).

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Control, Over Expression, Enzyme-linked Immunosorbent Assay

Journal: Molecular Medicine Reports

Article Title: S100A1 expression is increased in spinal cord injury and promotes inflammation, oxidative stress and apoptosis of PC12 cells induced by LPS via ERK signaling

doi: 10.3892/mmr.2022.12917

Figure Lengend Snippet: S100A1 regulates the oxidative stress levels of PC12 cells through the ERK signaling pathway. (A and B) ROS levels in the Control, LPS, LPS + S100A1 silencing/overexpression, LPS with S100A1 overexpression and EKR inhibitor groups. (C) mRNA and enzyme activity of CAT in the different groups. (D) mRNA and enzyme activity of MnSOD in the different groups. (E) The protein expression of Nrf2 in differnet groups, n=3. **P<0.01, ***P<0.001 vs. Control group. # P<0.05, ## P<0.01, ### P<0.001 vs. LPS group. & P<0.05, && P<0.01, &&& P<0.001 vs. LPS + si-S100A1. $ P<0.05, $$ P<0.01 vs. LPS + ov-S100A1 group. LPS, lipopolysaccharide; ROS, reactive oxygen species; CAT, catalase; MnSOD, manganese superoxide dismutase; ov, overexpression; si, short interfering; Nrf2, nuclear factor erythroid 2-related factor 2.

Article Snippet: In addition, the cells transfected with ov-S100A1 plasmid were treated with the ERK inhibitor, MK-8353 (100 nM, MedChemExpress), 12 h prior to exposure to LPS, as previously described ( ).

Techniques: Control, Over Expression, Activity Assay, Expressing

Journal: Molecular Medicine Reports

Article Title: S100A1 expression is increased in spinal cord injury and promotes inflammation, oxidative stress and apoptosis of PC12 cells induced by LPS via ERK signaling

doi: 10.3892/mmr.2022.12917

Figure Lengend Snippet: S100A1 regulates the apoptosis of PC12 cells through the ERK signaling pathway. (A and B) The apoptosis levels in the Control, LPS, LPS with S100A1 silencing/overexpression, LPS with S100A1 overexpression and EKR inhibitor groups. (C and D) The protein levels of Bax, Bcl2 and cleaved caspase-3 in the different groups. n=3. *P<0.05, ***P<0.001 vs. Control group. # P<0.05, ### P<0.001 vs. LPS group. && P<0.01, &&& P<0.001 vs. LPS + si-S100A1. $ P<0.05, $$ P<0.01, $$$ P<0.001 vs. LPS + ov-S100A1 group. LPS, lipopolysaccharide; ov, overexpression; si, short interfering.

Article Snippet: In addition, the cells transfected with ov-S100A1 plasmid were treated with the ERK inhibitor, MK-8353 (100 nM, MedChemExpress), 12 h prior to exposure to LPS, as previously described ( ).

Techniques: Control, Over Expression

Journal: PLoS ONE

Article Title: S100A1 as a Potential Diagnostic Biomarker for Assessing Cardiotoxicity and Implications for the Chemotherapy of Certain Cancers

doi: 10.1371/journal.pone.0145418

Figure Lengend Snippet: Panels show graphs for the markers TroponinI and S100A1. Multi group Kruskal Wallis test yielded P = 0.0280 and 0.0354, respectively. * indicates statistically significant difference against the control group C (see ).

Article Snippet: The serum S100A1 level was determined by using a rat protein S100A1 ELISA kit (Sensitivity 1.56 ng/ml; Cusabio Biotech, China), according to the manufacturers' instructions.

Techniques: Control

Journal: PLoS ONE

Article Title: S100A1 as a Potential Diagnostic Biomarker for Assessing Cardiotoxicity and Implications for the Chemotherapy of Certain Cancers

doi: 10.1371/journal.pone.0145418

Figure Lengend Snippet: Antioxidant and oxidant status in the heart tissues and serum markers obtained from the experimental groups.

Article Snippet: The serum S100A1 level was determined by using a rat protein S100A1 ELISA kit (Sensitivity 1.56 ng/ml; Cusabio Biotech, China), according to the manufacturers' instructions.

Techniques: Control

Journal: International journal of molecular medicine

Article Title: Oxidized S100A4 inhibits the activation of protein phosphatase 5 through S100A1 in MKN‑45 gastric carcinoma cells.

doi: 10.3892/ijmm.2014.1947

Figure Lengend Snippet: Figure 1. Oxidation of S100A4 and its effect on PP5 activation by S100A1. (A) Air‑oxidized and freshly prepared S100A4 were separated on a 15% Tricine SDS‑PAGE gel under reducing [dithiothreitol (DTT) (+)] or non‑reducing [(DTT(‑)] conditions, followed by staining with Coomassie Brilliant Blue (CBB). HMW, high molecular weight complex. Molecular weight markers are shown on the right (kDa). (B) PP5 activation assay. His‑PP5 (250 ng) was mixed with 100 µM phosphopeptide and various amounts of S100A1 in the presence of 1 mM CaCl2 or EGTA, followed by incubation for 10 min at 37˚C. Malachite green solution with 0.01% Tween‑20 was added and absorbance at 630 nm was measured. The amount of released phosphate was determined using a standard curve calculated from a known amount of phosphate. Each point represents the mean ± standard deviation (SD) of triplicate determinations. Fixed amounts (2.5 µg) of S100A1 and various amounts of (C) freshly prepared S100A4 or (D) air‑oxidized S100A4 were used for the PP5 activation assay in the presence of 1 mM CaCl2. Each point represents the mean ± SD of triplicate determinations.

Article Snippet: Rabbit anti-S100A1 antibody (NB100‐91955) was obtained from Novus Biologicals (Littleton, CO, USA).

Techniques: Activation Assay, Staining, High Molecular Weight, Molecular Weight, Phospho-proteomics, Incubation, Standard Deviation

Journal: International journal of molecular medicine

Article Title: Oxidized S100A4 inhibits the activation of protein phosphatase 5 through S100A1 in MKN‑45 gastric carcinoma cells.

doi: 10.3892/ijmm.2014.1947

Figure Lengend Snippet: Figure 2. Interaction between PP5 and S100 proteins. Protein binding analysis was performed using a SPR Biacore 2000 system. N‑ethyl‑N'‑(3‑diethylaminopropyl) carbodiimide, N‑hydroxysuccinimide, and ethanolamine‑HCl (Biacore) were used for amine coupling of PP5 or S100A1 to the dextran surface of the CM5 chip. His‑PP5 [2500 RU (pH 4.2)] or S100A1 [1400 RU (pH 4.5)] was immobilized in 10‑mM ammonium acetate. Various concentrations of recombinant S100 proteins were injected. Response curves were prepared for fitting by subtraction of the signal generated simultaneously on the control flow cell. Biacore sensorgrams were analyzed using BIAevaluation 4.1 software. (A) Freshly prepared S100A1 or S100A4. (B) Freshly prepared S100A1 firstly, followed by injection of S100A1 and S100A4. (C) S100A1 and S100A4 were injected, followed by S100A1 alone. (D) Various concentrations of air‑oxidized S100A4 were injected. (E) Fixed amount (5 µM) of S100A1 and various concentrations of air‑oxidized S100A4 were injected.

Article Snippet: Rabbit anti-S100A1 antibody (NB100‐91955) was obtained from Novus Biologicals (Littleton, CO, USA).

Techniques: Protein Binding, Recombinant, Injection, Generated, Control, Software

Journal: International journal of molecular medicine

Article Title: Oxidized S100A4 inhibits the activation of protein phosphatase 5 through S100A1 in MKN‑45 gastric carcinoma cells.

doi: 10.3892/ijmm.2014.1947

Figure Lengend Snippet: Figure 3. Effects of Cu‑oxidized S100A4 on the interaction with PP5 or S100A1 and PP5 activation. (A) Freshly prepared and Cu‑oxidized S100A4 were separated on 15% Tricine SDS‑PAGE gels under reducing [dithioth reitol (DTT) (+)] or non‑reducing [(DTT(‑)] conditions, and were stained with Coomassie Brilliant Blue (CBB). HMW, high molecular weight complex. Molecular weight markers are shown on the right (kDa). (B) Various amounts of Cu-oxidized S100A4 with or without fixed amount of S100A1 (2.5 µg) were used for the PP5 activation assay in the presence of 1 mM CaCl2. Each point represents the mean ± standard deviation (SD) of triplicate determina tions. (C) His‑PP5 was immobilized on the sensor chip and various amounts of freshly prepared S100A4 were applied. Binding was measured by surface plasmon resonance (SPR). (D) The binding of Cu‑oxidized S100A4 onto the PP5‑immobilized chip was measured. (E) S100A1 was immobilized on the sensor chip and various amounts of freshly prepared S100A4 were applied. Binding was measured by SPR analysis. (F) Binding of Cu‑oxidized S100A4 to the S100A1 immobilized on the chip was measured.

Article Snippet: Rabbit anti-S100A1 antibody (NB100‐91955) was obtained from Novus Biologicals (Littleton, CO, USA).

Techniques: Activation Assay, Staining, High Molecular Weight, Molecular Weight, Standard Deviation, Binding Assay, SPR Assay

Journal: International journal of molecular medicine

Article Title: Oxidized S100A4 inhibits the activation of protein phosphatase 5 through S100A1 in MKN‑45 gastric carcinoma cells.

doi: 10.3892/ijmm.2014.1947

Figure Lengend Snippet: Figure 4. Native PAGE analysis of S100‑PP5 interaction and the effect of H2O2 on S100A4 in MKN‑45 cells. (A) Approximately 10 µg each of S100A1, S100A4 or Cu‑oxidized S100A4 was separated on the gel (left three lanes). Same samples were incubated with PP5 and separated on the same gel (right three lanes). Gels were stained with Coomassie Brilliant Blue (CBB). Multiple bands formed by mixture of S100A1 and PP5 are indicated by closed arrow heads. (B) Approximately 10 µg each of freshly prepared S100A1, S100A4, Cu‑oxidized S100A4, S100A1 + S100A4 or S100A1 + Cu‑oxidized S100A4 were applied to the gels. Gels were stained with CBB. Electrophoretic mobility shifts of S100A1 by Cu‑oxidized S100A4 are indicated (open arrowhead). Multiple bands formed by mixture of S100A1 and PP5 are indicated by closed arrow heads. (C) Approximately 5.5x105 cells were plated on a 10‑cm dish and cultured for 3 days in standard medium. Medium was aspirated and washed with PBS and cells were exposed to the indicated concentrations of H2O2 ranging from 0 to 2 mM in the serum‑free medium for 90 min. Proteins were extracted and separated on 15% Tricine SDS‑PAGE under reducing [dithioth reitol (DTT) (+)] or non‑reducing [(DTT(‑)]conditions. Western blot analysis was performed using an anti‑S100A4 antibody. HMW, high molecular weight complexes. The band migrating with an apparent MW of 60 kDa is a non‑spe cific horseradish peroxidase (HRP)‑labeled secondary antibody‑reactive band. Molecular weight markers are shown on the right (kDa).

Article Snippet: Rabbit anti-S100A1 antibody (NB100‐91955) was obtained from Novus Biologicals (Littleton, CO, USA).

Techniques: Clear Native PAGE, Incubation, Staining, Cell Culture, Western Blot, High Molecular Weight, Molecular Weight

Journal: Translational cancer research

Article Title: S100A1 overexpression stimulates cell proliferation and is predictive of poor outcome in ovarian cancer.

doi: 10.21037/tcr-24-430

Figure Lengend Snippet: Figure 1 S100A1 upregulation is a common hallmark of human OC that is related to poorer patient outcomes. (A) S100A1 levels in OC

Article Snippet: 3% H2O2 for 30 min, and the sections were incubated with a mouse anti-S100A1 monoclonal antibody (Cell Signaling Technology, MA, Cat. 64095, dilution 1:100) at 4 °C overnight.

Techniques:

Journal: Translational cancer research

Article Title: S100A1 overexpression stimulates cell proliferation and is predictive of poor outcome in ovarian cancer.

doi: 10.21037/tcr-24-430

Figure Lengend Snippet: Figure 2 S100A1 enhances the proliferation and migration of OC cells in vitro. (A) S100A1 levels in OC cells. Replicates n=3. (B) qPCR was

Article Snippet: 3% H2O2 for 30 min, and the sections were incubated with a mouse anti-S100A1 monoclonal antibody (Cell Signaling Technology, MA, Cat. 64095, dilution 1:100) at 4 °C overnight.

Techniques: Migration, In Vitro

Journal: Translational cancer research

Article Title: S100A1 overexpression stimulates cell proliferation and is predictive of poor outcome in ovarian cancer.

doi: 10.21037/tcr-24-430

Figure Lengend Snippet: Figure 3 Genes correlated with S100A1 in OC determined by LinkedOmics (A) Spearman correlations. The green dots represent negative

Article Snippet: 3% H2O2 for 30 min, and the sections were incubated with a mouse anti-S100A1 monoclonal antibody (Cell Signaling Technology, MA, Cat. 64095, dilution 1:100) at 4 °C overnight.

Techniques:

Journal: Translational cancer research

Article Title: S100A1 overexpression stimulates cell proliferation and is predictive of poor outcome in ovarian cancer.

doi: 10.21037/tcr-24-430

Figure Lengend Snippet: Figure 4 Predicted functions of genes correlated with S100A1. (A) GO annotations (biological processes) and (B) KEGG pathways. (C)

Article Snippet: 3% H2O2 for 30 min, and the sections were incubated with a mouse anti-S100A1 monoclonal antibody (Cell Signaling Technology, MA, Cat. 64095, dilution 1:100) at 4 °C overnight.

Techniques:

Journal: Translational cancer research

Article Title: S100A1 overexpression stimulates cell proliferation and is predictive of poor outcome in ovarian cancer.

doi: 10.21037/tcr-24-430

Figure Lengend Snippet: Figure 5 The effect of S100A1 knockout on mouse xenograft tumor growth. Tumor photo (A) and tumor growth curves (B) for nude mice

Article Snippet: 3% H2O2 for 30 min, and the sections were incubated with a mouse anti-S100A1 monoclonal antibody (Cell Signaling Technology, MA, Cat. 64095, dilution 1:100) at 4 °C overnight.

Techniques: Knock-Out