Journal: International Journal of Molecular Sciences

Article Title: Inducible Expression of both ermB and ermT Conferred High Macrolide Resistance in Streptococcus gallolyticus subsp. pasteurianus Isolates in China

doi: 10.3390/ijms17101599

Figure Lengend Snippet: Minimum inhibitory concentrations of six S. gallolyticus subsp. pasteurianus isolates.

Article Snippet: S. gallolyticus subsp. pasteurianus ATCC 43144 and S. aureus ATCC 29213 were used as controls [ , ].

Techniques:

Journal: PLoS ONE

Article Title: Transcriptome analysis of Streptococcus gallolyticus subsp. gallolyticus in interaction with THP-1 macrophage-like cells

doi: 10.1371/journal.pone.0180044

Figure Lengend Snippet: List of bacterial strains used in this study with the source.

Article Snippet: Streptococcus gallolyticus subsp. gallolyticus , UCN34 , human, IE patient , ATCC.

Techniques:

Journal: PLoS ONE

Article Title: Transcriptome analysis of Streptococcus gallolyticus subsp. gallolyticus in interaction with THP-1 macrophage-like cells

doi: 10.1371/journal.pone.0180044

Figure Lengend Snippet: Ratio of bacteria to macrophages in microscopic analysis and response of THP-1 macrophages to Streptococcus gallolyticus subsp. gallolyticus strains and Staphylococcus aureus ATCC 25923. 1+2) Microscopic analysis revealed the ratio of bacteria counted (without differentiation between dead and alive bacteria) to macrophages with the distinction between bacteria within and outside macrophages (bacteria/macrophage; counted macrophages are set as 1; Fig A and supplemental method in S1 File ; n = 100 macrophages). 3) The oxidative reaction of macrophages to bacteria was detected by fluorescence signaling (DCF) at time point t = 0 h. H 2 O 2 (1 mM) was used as stimulation control (Fig B1 in S1 File ). 3) Cytotoxicity of bacteria to THP-1 macrophages compared to control (lysis of THP-1 macrophages by lysis buffer was set as 100%) was analyzed by LDH-assay 5 h after phagocytosis (Fig B2 in S1 File ). 4–6) Gene expression of cytokines in THP-1 macrophages was determined by real-time PCR after stimulation with the different bacteria (t = 5 h); the control without bacterial stimulation was set as one (Fig C in S1 File ). Results of statistical analysis (Mann-Whitney U test) of individual strains are arranged in tabular form. Only significant differences between strains and bacterial species are represented. Arbitrary units = au; SA = Staphylococcus aureus ; bac = bacterial cells; I = intracellular; e = extracellular; m = macrophage; n = 3.

Article Snippet: Streptococcus gallolyticus subsp. gallolyticus , UCN34 , human, IE patient , ATCC.

Techniques: Bacteria, Fluorescence, Control, Lysis, Gene Expression, Real-time Polymerase Chain Reaction, Mann-Whitney U-Test

Journal: PLoS ONE

Article Title: Transcriptome analysis of Streptococcus gallolyticus subsp. gallolyticus in interaction with THP-1 macrophage-like cells

doi: 10.1371/journal.pone.0180044

Figure Lengend Snippet: Differentially regulated genes of S . gallolyticus subsp. gallolyticus BAA-2069 and UCN34 through phagocytosis by THP-1 macrophages in microarray analysis sorted by function. Changes in gene expression after phagocytosis at time point 0 h and 5 h are shown compared to the transcriptome at time point -1 h (attachment to macrophages). Fluorescence intensities of the microarrays were quantile normalized and computed log2 values and fold changes with significances (Welch’s test) are listed; n = 3; n. r. = not regulated.

Article Snippet: Streptococcus gallolyticus subsp. gallolyticus , UCN34 , human, IE patient , ATCC.

Techniques: Microarray, Gene Expression, Fluorescence, Starch, Histone Deacetylase Assay, Maltodextrin, Membrane, Transduction, Ubiquitin Proteomics

Journal: PLoS ONE

Article Title: Transcriptome analysis of Streptococcus gallolyticus subsp. gallolyticus in interaction with THP-1 macrophage-like cells

doi: 10.1371/journal.pone.0180044

Figure Lengend Snippet: The genes whose expression were increased (white) or decreased (black) after phagocytosis (0 h and 5 h together) compared to -1 h (attachment of bacteria) are represented. The number of genes which were regulated in the S . gallolyticus subsp. gallolyticus strains BAA-2069 and UCN34 through phagocytic uptake by THP-1 macrophages are displayed on the x-axis. The functional categories in which the genes are sorted are displayed on the y-axis.

Article Snippet: Streptococcus gallolyticus subsp. gallolyticus , UCN34 , human, IE patient , ATCC.

Techniques: Expressing, Bacteria, Functional Assay

Journal:

Article Title: Ability of Lysozyme and 2-Deoxyglucose To Differentiate Human and Bovine Streptococcus bovis Strains

doi: 10.1128/JCM.41.8.3951-3954.2003

Figure Lengend Snippet: Origin of bacterial strains

Article Snippet: ; prior to 1989 17 ATCC 43143, ATCC 43144 Human blood 23 ATCC 49133, ATCC 49147 Human clinical isolates; unknown origin 23 Open in a separate window Origin of bacterial strains table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Strain Biotype Substrate utilization a RFLP BOX-PCR Starch Raffinose Lactose Mannitol Bovine 3-01 II/1 + + + − A B10 3-02 II/1 + + + − A B10 3-03 II/1 + + + − A B9 3-04 II/1 + + + − A B9 3-05 II/1 + + + − A B10 3-06 II/1 + + + − A B10 3-07 II/1 + + + − A B11 3-08 II/1 + + + − A B10 3-09 II/1 + + + − A B10 3-10 II/1 + + + − A B5 5-02 II/1 + + + − A B7 5-04 II/1 + − + − A B9 5-05 II/1 + + + − A B13 5-06 II/1 + + + − A B4 5-07 II/1 + + + − A B9 5-08 II/1 + + + − A B9 5-09 II/1 + + + − A B9 5-11 II/1 + + + − A B13 5-12 II/1 + − + − A B9 5-17 II/1 + + + − A B9 7-02 II/1 + + + − A B9 7-04 II/1 + + + − A B9 7-06 II/1 + + + − A B9 7-10 II/1 + + + − A B9 7-14 II/1 + + + − A B9 7-17 II/1 + + + − A B10 7-20 II/1 + + + − A B9 7-21 II/1 + + + − A B9 7-24 II/1 + + + − A B9 JB1 II/1 + + + − A B2 K27FF4 II/1 + + + − A B1 26 II/1 + + + − A B8 581AXY2 II/1 + + + − A B8 ATCC 33317 II/1 + + + − A B11 ATCC 15351 II/1 + + + − A B3 HC5 II/1 + + + − A B12 Human FM II/1 W W + − A H1 RG I + + + + A H4 V1387 I + + + + A H4 V1388 NA b − + + + A H4 V1477 I + + + + A H4 6 II/1 W + + − A H1 1314 I + + + + A H4 1499 II/1 + W − − A H1 2703 I + + W + A H2 6448 II/1 + + + − A H5 9410 I + + + + A H4 ATCC 43143 NA − + + + A H4 ATCC 43144 II/1 + + + − A B10 ATCC 49133 II/1 W + + − A H3 ATCC 49147 I W + + + A H4 Open in a separate window a The ability to utilize starch, raffinose, lactose, and mannitol is shown as follows: +, utilizes; −, cannot utilize; W, weak growth.

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