Journal: International Journal of Molecular Sciences

Article Title: Inducible Expression of both ermB and ermT Conferred High Macrolide Resistance in Streptococcus gallolyticus subsp. pasteurianus Isolates in China

doi: 10.3390/ijms17101599

Figure Lengend Snippet: Minimum inhibitory concentrations of six S. gallolyticus subsp. pasteurianus isolates.

Article Snippet: S. gallolyticus subsp. pasteurianus ATCC 43144 and S. aureus ATCC 29213 were used as controls [ , ].

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Article Title: Ability of Lysozyme and 2-Deoxyglucose To Differentiate Human and Bovine Streptococcus bovis Strains

doi: 10.1128/JCM.41.8.3951-3954.2003

Figure Lengend Snippet: Origin of bacterial strains

Article Snippet: ; prior to 1989 17 ATCC 43143, ATCC 43144 Human blood 23 ATCC 49133, ATCC 49147 Human clinical isolates; unknown origin 23 Open in a separate window Origin of bacterial strains table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Strain Biotype Substrate utilization a RFLP BOX-PCR Starch Raffinose Lactose Mannitol Bovine 3-01 II/1 + + + − A B10 3-02 II/1 + + + − A B10 3-03 II/1 + + + − A B9 3-04 II/1 + + + − A B9 3-05 II/1 + + + − A B10 3-06 II/1 + + + − A B10 3-07 II/1 + + + − A B11 3-08 II/1 + + + − A B10 3-09 II/1 + + + − A B10 3-10 II/1 + + + − A B5 5-02 II/1 + + + − A B7 5-04 II/1 + − + − A B9 5-05 II/1 + + + − A B13 5-06 II/1 + + + − A B4 5-07 II/1 + + + − A B9 5-08 II/1 + + + − A B9 5-09 II/1 + + + − A B9 5-11 II/1 + + + − A B13 5-12 II/1 + − + − A B9 5-17 II/1 + + + − A B9 7-02 II/1 + + + − A B9 7-04 II/1 + + + − A B9 7-06 II/1 + + + − A B9 7-10 II/1 + + + − A B9 7-14 II/1 + + + − A B9 7-17 II/1 + + + − A B10 7-20 II/1 + + + − A B9 7-21 II/1 + + + − A B9 7-24 II/1 + + + − A B9 JB1 II/1 + + + − A B2 K27FF4 II/1 + + + − A B1 26 II/1 + + + − A B8 581AXY2 II/1 + + + − A B8 ATCC 33317 II/1 + + + − A B11 ATCC 15351 II/1 + + + − A B3 HC5 II/1 + + + − A B12 Human FM II/1 W W + − A H1 RG I + + + + A H4 V1387 I + + + + A H4 V1388 NA b − + + + A H4 V1477 I + + + + A H4 6 II/1 W + + − A H1 1314 I + + + + A H4 1499 II/1 + W − − A H1 2703 I + + W + A H2 6448 II/1 + + + − A H5 9410 I + + + + A H4 ATCC 43143 NA − + + + A H4 ATCC 43144 II/1 + + + − A B10 ATCC 49133 II/1 W + + − A H3 ATCC 49147 I W + + + A H4 Open in a separate window a The ability to utilize starch, raffinose, lactose, and mannitol is shown as follows: +, utilizes; −, cannot utilize; W, weak growth.

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Journal: Journal of molecular biology

Article Title: Conformational Change of the Hairpin-like-structured Robo2 Ectodomain Allows NELL1/2 Binding.

doi: 10.1016/j.jmb.2022.167777

Figure Lengend Snippet: Figure 4. FRET-based indicators to assess the topological conformation of the Robo2 ectodomain. (A) Schematic diagrams of the intramolecular FRET constructs. Gamillus/GFP and mTurquoise2/CFP were fused to the N- and C-termini of the Robo2 ectodomain, respectively. A deletion construct of both the Ig1 and FNIII3 domains was prepared to decrease the distance between Gamillus and mTurquoise2. (B) Schematic overview of the FRET experiments. If Gamillus and mTurquoise2 are in close proximity due to conformational change of the Robo2 ectodomain, FRET (emission of 515-nm light after excitation with 430-nm light) may occur. (C) Emission scans of Robo2-ECD and Robo2-ECD/DIg1&FNIII3 constructs at pH 7.0. The excitation wavelength was 430 nm and emission was measured at 460–560 nm. (D) The FRET ratio was defined as the ratio of emission intensity of Gamillus to the emission intensity of mTurquoise2 (515 ± 5 nm/475 ± 5 nm). Each value represents the mean ± SD of at least triplicate results. *, p < 0.05 (Student’s t-test).

Article Snippet: Gamillus/ pcDNA3 was a gift from Takeharu Nagai (Addgene plasmid # 124837).38 pLifeAct- mTurquoise2 was a gift from Dorus Gadella (Addgene plasmid # 36201).37 Deletion mutants lacking the coding sequence for both the Ig1 domain (residues 43–144) and the FNIII3 domain (residues 740–826) or only Ig1 domain were generated by inverse PCR and designated as Gam:Rob o2-ECD/DIg1&FNIII3:mTq2 and Gam:Robo2ECD/DIg1:mTq2, respectively.

Techniques: Construct