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Image Search Results
Journal: Hepatology (Baltimore, Md.)
Article Title: Hepatitis B Virus Virions Produced Under Nucleos(t)ide Analogue Treatment Are Mainly Not Infectious Because of Irreversible DNA Chain Termination
doi: 10.1002/hep.30844
Figure Lengend Snippet: The virions produced under NA treatment were mainly disabled to infect PHHs. (A,B) HBeAg and HBsAg levels in the media of PHH cultures infected with virions produced by HepAD38 cells treated with DMSO (NT) or 2.5 μM of ETV were detected 2, 4, and 6 days postinfection, respectively. (C,D) HBeAg and HBsAg levels in the media of PHH cultures infected with virions from sera of CHB patients with or without NA therapy were detected 2, 4, and 6 days postinfection, respectively. Negative control (NC) is media harvested from uninfected cells. The dashed lines indicated the LLoD of HBeAg and HBsAg, respectively. PE IU/mL, Paul Ehrlich Institute Units.
Article Snippet: Media of HepG2‐NTCP and PHH cultures were harvested at 2, 4, and 6 days post‐HBV infection, and levels of
Techniques: Produced, Infection, Negative Control
Journal: Hepatology (Baltimore, Md.)
Article Title: Hepatitis B Virus Virions Produced Under Nucleos(t)ide Analogue Treatment Are Mainly Not Infectious Because of Irreversible DNA Chain Termination
doi: 10.1002/hep.30844
Figure Lengend Snippet: HBV virions produced under NA treatment were mainly disabled to infect HepG2‐NTCP cells. (A) Schematic for the treatment of HepAD38 cells and HBV‐DNA quantification of the supernatant with or without ETV treatment. (B) HBV capsid level with adjusted HBV DNA was detected by western blotting of HBV core (HBc) protein. (C) Immunofluorescence demonstration of NTCP expression in HepG2‐NTCP cells cultured in the presence of Dox (right). (D‐F) HBeAg, HBsAg, and HBV DNA in the media of HepG2‐NTCP cultures infected with virions produced by HepAD38 cells treated with DMSO (NT) or ETV at the indicated MOI were tested. Negative control (NC) is media harvested from uninfected cells. (G) HBeAg levels in the media of HepG2‐NTCP cultures infected with virions produced by HepAD38 cells treated with different concentrations (0, 100, 300, 600, 1,200, and 2,500 nM) of ETV were detected 2, 4, and 6 days postinfection, respectively. (H) The IC 50 of ETV in inhibiting progeny HBV infectivity was calculated by nonlinear regression of log (inhibitor) versus response. PE IU/mL, Paul Ehrlich Institute Units.
Article Snippet: Media of HepG2‐NTCP and PHH cultures were harvested at 2, 4, and 6 days post‐HBV infection, and levels of
Techniques: Produced, Western Blot, Immunofluorescence, Expressing, Cell Culture, Infection, Negative Control
Journal: The Tohoku journal of experimental medicine
Article Title: Osteopontin as a key mediator for vasculogenic mimicry in hepatocellular carcinoma.
doi: 10.1620/tjem.224.29
Figure Lengend Snippet: Fig. 7. Immunohistochemical staining for VM in HCC and normal liver tissues. (A) VM expression was identified in a HCC lesion by laminin immunostain (red arrow). (B) The same HCC lesion in (A) showing VM expression by laminin im- munostain did not express the endothelial cell marker CD31. Hepatic sinusoids were CD34 positive (yellow arrow). (C) The same HCC lesion in (A) was positive for hepatocyte. (D) A HCC lesion without VM expression by laminin im- munostain. Bars = 50 μm.
Article Snippet: After washing in 0.1 M phosphate-buffered saline (PBS), pH 7.4, and exposure to 3% normal goat serum for 20 min to reduce non-specific binding, the slides were incubated for 2 h at 37°C with a 1:100 dilution of anti-laminin monoclonal antibody (Millipore), or a 1:100 dilution of anti-CD34 antibody (Santa Cruz Biotechnology, Santa Cruz, CA), or a working solution of
Techniques: Immunohistochemical staining, Staining, Expressing, Marker