Journal: The FASEB Journal

Article Title: Single Cell Analysis Reveals the Presence of Novel Intermediate Cells in Both Mice and Patients With Severe MASLD

doi: 10.1096/fj.202504360R

Figure Lengend Snippet: Spatial validation of sc‐RNAseq and localization of intermediate cells in liver specimens. Validation of intermediate cell populations across disease severity by analyzing the co‐expression patterns of specific markers using MACSima Imaging System. DAPI staining has been employed to highlight nuclei (scale bar 20 μm; scale bar inset 4 μm).

Article Snippet: Liver frozen embedded tissues, 4 μm thick, were mounted on SuperFrost Plus slides and fixed with a 4% paraformaldehyde solution for 10 min at RT, rinsed three times with MACSima Running Buffer (130‐121‐565, Miltenyi Biotec, Bergisch Gladbach, Germany), and placed onto MACSwell Four Imaging Frames (130‐124‐676, Miltenyi Biotec).

Techniques: Biomarker Discovery, RNA sequencing, Expressing, Imaging, Staining

Journal: The FASEB Journal

Article Title: Single Cell Analysis Reveals the Presence of Novel Intermediate Cells in Both Mice and Patients With Severe MASLD

doi: 10.1096/fj.202504360R

Figure Lengend Snippet: Spatial visualization of intermediate cells in human liver specimens. Validation of intermediate cell populations in human hepatic samples of normal liver and MASH‐fibrosis by analyzing the co‐expression patterns of specific markers by using MACSima Imaging System. DAPI staining has been employed to highlight nuclei (scale bar 20 μm; scale bar inset 4 μm).

Article Snippet: Liver frozen embedded tissues, 4 μm thick, were mounted on SuperFrost Plus slides and fixed with a 4% paraformaldehyde solution for 10 min at RT, rinsed three times with MACSima Running Buffer (130‐121‐565, Miltenyi Biotec, Bergisch Gladbach, Germany), and placed onto MACSwell Four Imaging Frames (130‐124‐676, Miltenyi Biotec).

Techniques: Biomarker Discovery, Expressing, Imaging, Staining