rt cdna synthesis kit Search Results


96
Toyobo rt ramda cdna synthesis kit
Establishment of a TaqMan-based system to quantify allele-specific transcripts from the X chromosomes (A) Isolation of hybrid MEFs for XCR analyses. (B) TaqMan probes that discriminate transcripts from B6 and Sp alleles. TaqMan probes in a single reaction recognize their cognate allele-specific transcripts, which differ by SNPs or an MNP, and emit fluorescence (VIC or FAM). (C) Mouse X chromosome showing the locations of Lamp2 , Atrx , and the X-inactivation center (Xic). (D) Linear correlation between the ratio of the allele-specific <t>cDNA</t> amounts and the ratio of emitted fluorescence for each allele. cDNAs derived from homozygous B6 and Sp mice were mixed at five different ratios, and qRT-PCR was performed using TaqMan probes for the Lamp2 or Atrx gene. The square of the correlation coefficient is indicated by R 2 . (E) Relative abundance of allele-specific transcripts in drug-selected MEFs. The relative abundances of allele-specific transcripts in HAT- or 6-TG-selected bsMEFs were calculated by using TaqMan probes for Lamp2 or Atrx . Data represent mean ± SEM of at least three independent experiments. ∗ p < 0.05 and ∗∗ p < 0.01. n.s., not significant.
Rt Ramda Cdna Synthesis Kit, supplied by Toyobo, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Solis BioDyne firescript cdna synthesis kit
Establishment of a TaqMan-based system to quantify allele-specific transcripts from the X chromosomes (A) Isolation of hybrid MEFs for XCR analyses. (B) TaqMan probes that discriminate transcripts from B6 and Sp alleles. TaqMan probes in a single reaction recognize their cognate allele-specific transcripts, which differ by SNPs or an MNP, and emit fluorescence (VIC or FAM). (C) Mouse X chromosome showing the locations of Lamp2 , Atrx , and the X-inactivation center (Xic). (D) Linear correlation between the ratio of the allele-specific <t>cDNA</t> amounts and the ratio of emitted fluorescence for each allele. cDNAs derived from homozygous B6 and Sp mice were mixed at five different ratios, and qRT-PCR was performed using TaqMan probes for the Lamp2 or Atrx gene. The square of the correlation coefficient is indicated by R 2 . (E) Relative abundance of allele-specific transcripts in drug-selected MEFs. The relative abundances of allele-specific transcripts in HAT- or 6-TG-selected bsMEFs were calculated by using TaqMan probes for Lamp2 or Atrx . Data represent mean ± SEM of at least three independent experiments. ∗ p < 0.05 and ∗∗ p < 0.01. n.s., not significant.
Firescript Cdna Synthesis Kit, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Toyobo quantaccuracytm rt ramdm cdna synthesis kit
Establishment of a TaqMan-based system to quantify allele-specific transcripts from the X chromosomes (A) Isolation of hybrid MEFs for XCR analyses. (B) TaqMan probes that discriminate transcripts from B6 and Sp alleles. TaqMan probes in a single reaction recognize their cognate allele-specific transcripts, which differ by SNPs or an MNP, and emit fluorescence (VIC or FAM). (C) Mouse X chromosome showing the locations of Lamp2 , Atrx , and the X-inactivation center (Xic). (D) Linear correlation between the ratio of the allele-specific <t>cDNA</t> amounts and the ratio of emitted fluorescence for each allele. cDNAs derived from homozygous B6 and Sp mice were mixed at five different ratios, and qRT-PCR was performed using TaqMan probes for the Lamp2 or Atrx gene. The square of the correlation coefficient is indicated by R 2 . (E) Relative abundance of allele-specific transcripts in drug-selected MEFs. The relative abundances of allele-specific transcripts in HAT- or 6-TG-selected bsMEFs were calculated by using TaqMan probes for Lamp2 or Atrx . Data represent mean ± SEM of at least three independent experiments. ∗ p < 0.05 and ∗∗ p < 0.01. n.s., not significant.
Quantaccuracytm Rt Ramdm Cdna Synthesis Kit, supplied by Toyobo, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Solis BioDyne reverse transcriptase
Establishment of a TaqMan-based system to quantify allele-specific transcripts from the X chromosomes (A) Isolation of hybrid MEFs for XCR analyses. (B) TaqMan probes that discriminate transcripts from B6 and Sp alleles. TaqMan probes in a single reaction recognize their cognate allele-specific transcripts, which differ by SNPs or an MNP, and emit fluorescence (VIC or FAM). (C) Mouse X chromosome showing the locations of Lamp2 , Atrx , and the X-inactivation center (Xic). (D) Linear correlation between the ratio of the allele-specific <t>cDNA</t> amounts and the ratio of emitted fluorescence for each allele. cDNAs derived from homozygous B6 and Sp mice were mixed at five different ratios, and qRT-PCR was performed using TaqMan probes for the Lamp2 or Atrx gene. The square of the correlation coefficient is indicated by R 2 . (E) Relative abundance of allele-specific transcripts in drug-selected MEFs. The relative abundances of allele-specific transcripts in HAT- or 6-TG-selected bsMEFs were calculated by using TaqMan probes for Lamp2 or Atrx . Data represent mean ± SEM of at least three independent experiments. ∗ p < 0.05 and ∗∗ p < 0.01. n.s., not significant.
Reverse Transcriptase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Qiagen universal rt microrna pcr; polyadenylation cdna synthesis kit
Establishment of a TaqMan-based system to quantify allele-specific transcripts from the X chromosomes (A) Isolation of hybrid MEFs for XCR analyses. (B) TaqMan probes that discriminate transcripts from B6 and Sp alleles. TaqMan probes in a single reaction recognize their cognate allele-specific transcripts, which differ by SNPs or an MNP, and emit fluorescence (VIC or FAM). (C) Mouse X chromosome showing the locations of Lamp2 , Atrx , and the X-inactivation center (Xic). (D) Linear correlation between the ratio of the allele-specific <t>cDNA</t> amounts and the ratio of emitted fluorescence for each allele. cDNAs derived from homozygous B6 and Sp mice were mixed at five different ratios, and qRT-PCR was performed using TaqMan probes for the Lamp2 or Atrx gene. The square of the correlation coefficient is indicated by R 2 . (E) Relative abundance of allele-specific transcripts in drug-selected MEFs. The relative abundances of allele-specific transcripts in HAT- or 6-TG-selected bsMEFs were calculated by using TaqMan probes for Lamp2 or Atrx . Data represent mean ± SEM of at least three independent experiments. ∗ p < 0.05 and ∗∗ p < 0.01. n.s., not significant.
Universal Rt Microrna Pcr; Polyadenylation Cdna Synthesis Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Servicebio Inc rt first strand cdna synthesis kit
Establishment of a TaqMan-based system to quantify allele-specific transcripts from the X chromosomes (A) Isolation of hybrid MEFs for XCR analyses. (B) TaqMan probes that discriminate transcripts from B6 and Sp alleles. TaqMan probes in a single reaction recognize their cognate allele-specific transcripts, which differ by SNPs or an MNP, and emit fluorescence (VIC or FAM). (C) Mouse X chromosome showing the locations of Lamp2 , Atrx , and the X-inactivation center (Xic). (D) Linear correlation between the ratio of the allele-specific <t>cDNA</t> amounts and the ratio of emitted fluorescence for each allele. cDNAs derived from homozygous B6 and Sp mice were mixed at five different ratios, and qRT-PCR was performed using TaqMan probes for the Lamp2 or Atrx gene. The square of the correlation coefficient is indicated by R 2 . (E) Relative abundance of allele-specific transcripts in drug-selected MEFs. The relative abundances of allele-specific transcripts in HAT- or 6-TG-selected bsMEFs were calculated by using TaqMan probes for Lamp2 or Atrx . Data represent mean ± SEM of at least three independent experiments. ∗ p < 0.05 and ∗∗ p < 0.01. n.s., not significant.
Rt First Strand Cdna Synthesis Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Servicebio Inc rt first strand cdna synthesis kit g3330
Establishment of a TaqMan-based system to quantify allele-specific transcripts from the X chromosomes (A) Isolation of hybrid MEFs for XCR analyses. (B) TaqMan probes that discriminate transcripts from B6 and Sp alleles. TaqMan probes in a single reaction recognize their cognate allele-specific transcripts, which differ by SNPs or an MNP, and emit fluorescence (VIC or FAM). (C) Mouse X chromosome showing the locations of Lamp2 , Atrx , and the X-inactivation center (Xic). (D) Linear correlation between the ratio of the allele-specific <t>cDNA</t> amounts and the ratio of emitted fluorescence for each allele. cDNAs derived from homozygous B6 and Sp mice were mixed at five different ratios, and qRT-PCR was performed using TaqMan probes for the Lamp2 or Atrx gene. The square of the correlation coefficient is indicated by R 2 . (E) Relative abundance of allele-specific transcripts in drug-selected MEFs. The relative abundances of allele-specific transcripts in HAT- or 6-TG-selected bsMEFs were calculated by using TaqMan probes for Lamp2 or Atrx . Data represent mean ± SEM of at least three independent experiments. ∗ p < 0.05 and ∗∗ p < 0.01. n.s., not significant.
Rt First Strand Cdna Synthesis Kit G3330, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
EuroClone wonder rt cdna synthesis kit
Establishment of a TaqMan-based system to quantify allele-specific transcripts from the X chromosomes (A) Isolation of hybrid MEFs for XCR analyses. (B) TaqMan probes that discriminate transcripts from B6 and Sp alleles. TaqMan probes in a single reaction recognize their cognate allele-specific transcripts, which differ by SNPs or an MNP, and emit fluorescence (VIC or FAM). (C) Mouse X chromosome showing the locations of Lamp2 , Atrx , and the X-inactivation center (Xic). (D) Linear correlation between the ratio of the allele-specific <t>cDNA</t> amounts and the ratio of emitted fluorescence for each allele. cDNAs derived from homozygous B6 and Sp mice were mixed at five different ratios, and qRT-PCR was performed using TaqMan probes for the Lamp2 or Atrx gene. The square of the correlation coefficient is indicated by R 2 . (E) Relative abundance of allele-specific transcripts in drug-selected MEFs. The relative abundances of allele-specific transcripts in HAT- or 6-TG-selected bsMEFs were calculated by using TaqMan probes for Lamp2 or Atrx . Data represent mean ± SEM of at least three independent experiments. ∗ p < 0.05 and ∗∗ p < 0.01. n.s., not significant.
Wonder Rt Cdna Synthesis Kit, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Genet Bio Inc suprimescript cdna synthesis kit
Establishment of a TaqMan-based system to quantify allele-specific transcripts from the X chromosomes (A) Isolation of hybrid MEFs for XCR analyses. (B) TaqMan probes that discriminate transcripts from B6 and Sp alleles. TaqMan probes in a single reaction recognize their cognate allele-specific transcripts, which differ by SNPs or an MNP, and emit fluorescence (VIC or FAM). (C) Mouse X chromosome showing the locations of Lamp2 , Atrx , and the X-inactivation center (Xic). (D) Linear correlation between the ratio of the allele-specific <t>cDNA</t> amounts and the ratio of emitted fluorescence for each allele. cDNAs derived from homozygous B6 and Sp mice were mixed at five different ratios, and qRT-PCR was performed using TaqMan probes for the Lamp2 or Atrx gene. The square of the correlation coefficient is indicated by R 2 . (E) Relative abundance of allele-specific transcripts in drug-selected MEFs. The relative abundances of allele-specific transcripts in HAT- or 6-TG-selected bsMEFs were calculated by using TaqMan probes for Lamp2 or Atrx . Data represent mean ± SEM of at least three independent experiments. ∗ p < 0.05 and ∗∗ p < 0.01. n.s., not significant.
Suprimescript Cdna Synthesis Kit, supplied by Genet Bio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega pcr cdna synthesis kit
Establishment of a TaqMan-based system to quantify allele-specific transcripts from the X chromosomes (A) Isolation of hybrid MEFs for XCR analyses. (B) TaqMan probes that discriminate transcripts from B6 and Sp alleles. TaqMan probes in a single reaction recognize their cognate allele-specific transcripts, which differ by SNPs or an MNP, and emit fluorescence (VIC or FAM). (C) Mouse X chromosome showing the locations of Lamp2 , Atrx , and the X-inactivation center (Xic). (D) Linear correlation between the ratio of the allele-specific <t>cDNA</t> amounts and the ratio of emitted fluorescence for each allele. cDNAs derived from homozygous B6 and Sp mice were mixed at five different ratios, and qRT-PCR was performed using TaqMan probes for the Lamp2 or Atrx gene. The square of the correlation coefficient is indicated by R 2 . (E) Relative abundance of allele-specific transcripts in drug-selected MEFs. The relative abundances of allele-specific transcripts in HAT- or 6-TG-selected bsMEFs were calculated by using TaqMan probes for Lamp2 or Atrx . Data represent mean ± SEM of at least three independent experiments. ∗ p < 0.05 and ∗∗ p < 0.01. n.s., not significant.
Pcr Cdna Synthesis Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tiangen biotech co cdna first-strand synthesis kit quantscript rt kit
Establishment of a TaqMan-based system to quantify allele-specific transcripts from the X chromosomes (A) Isolation of hybrid MEFs for XCR analyses. (B) TaqMan probes that discriminate transcripts from B6 and Sp alleles. TaqMan probes in a single reaction recognize their cognate allele-specific transcripts, which differ by SNPs or an MNP, and emit fluorescence (VIC or FAM). (C) Mouse X chromosome showing the locations of Lamp2 , Atrx , and the X-inactivation center (Xic). (D) Linear correlation between the ratio of the allele-specific <t>cDNA</t> amounts and the ratio of emitted fluorescence for each allele. cDNAs derived from homozygous B6 and Sp mice were mixed at five different ratios, and qRT-PCR was performed using TaqMan probes for the Lamp2 or Atrx gene. The square of the correlation coefficient is indicated by R 2 . (E) Relative abundance of allele-specific transcripts in drug-selected MEFs. The relative abundances of allele-specific transcripts in HAT- or 6-TG-selected bsMEFs were calculated by using TaqMan probes for Lamp2 or Atrx . Data represent mean ± SEM of at least three independent experiments. ∗ p < 0.05 and ∗∗ p < 0.01. n.s., not significant.
Cdna First Strand Synthesis Kit Quantscript Rt Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Servicebio Inc swescript rt i first-strand cdna synthesis kit
Establishment of a TaqMan-based system to quantify allele-specific transcripts from the X chromosomes (A) Isolation of hybrid MEFs for XCR analyses. (B) TaqMan probes that discriminate transcripts from B6 and Sp alleles. TaqMan probes in a single reaction recognize their cognate allele-specific transcripts, which differ by SNPs or an MNP, and emit fluorescence (VIC or FAM). (C) Mouse X chromosome showing the locations of Lamp2 , Atrx , and the X-inactivation center (Xic). (D) Linear correlation between the ratio of the allele-specific <t>cDNA</t> amounts and the ratio of emitted fluorescence for each allele. cDNAs derived from homozygous B6 and Sp mice were mixed at five different ratios, and qRT-PCR was performed using TaqMan probes for the Lamp2 or Atrx gene. The square of the correlation coefficient is indicated by R 2 . (E) Relative abundance of allele-specific transcripts in drug-selected MEFs. The relative abundances of allele-specific transcripts in HAT- or 6-TG-selected bsMEFs were calculated by using TaqMan probes for Lamp2 or Atrx . Data represent mean ± SEM of at least three independent experiments. ∗ p < 0.05 and ∗∗ p < 0.01. n.s., not significant.
Swescript Rt I First Strand Cdna Synthesis Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Establishment of a TaqMan-based system to quantify allele-specific transcripts from the X chromosomes (A) Isolation of hybrid MEFs for XCR analyses. (B) TaqMan probes that discriminate transcripts from B6 and Sp alleles. TaqMan probes in a single reaction recognize their cognate allele-specific transcripts, which differ by SNPs or an MNP, and emit fluorescence (VIC or FAM). (C) Mouse X chromosome showing the locations of Lamp2 , Atrx , and the X-inactivation center (Xic). (D) Linear correlation between the ratio of the allele-specific cDNA amounts and the ratio of emitted fluorescence for each allele. cDNAs derived from homozygous B6 and Sp mice were mixed at five different ratios, and qRT-PCR was performed using TaqMan probes for the Lamp2 or Atrx gene. The square of the correlation coefficient is indicated by R 2 . (E) Relative abundance of allele-specific transcripts in drug-selected MEFs. The relative abundances of allele-specific transcripts in HAT- or 6-TG-selected bsMEFs were calculated by using TaqMan probes for Lamp2 or Atrx . Data represent mean ± SEM of at least three independent experiments. ∗ p < 0.05 and ∗∗ p < 0.01. n.s., not significant.

Journal: Stem Cell Reports

Article Title: Early reactivation of clustered genes on the inactive X chromosome during somatic cell reprogramming

doi: 10.1016/j.stemcr.2021.11.008

Figure Lengend Snippet: Establishment of a TaqMan-based system to quantify allele-specific transcripts from the X chromosomes (A) Isolation of hybrid MEFs for XCR analyses. (B) TaqMan probes that discriminate transcripts from B6 and Sp alleles. TaqMan probes in a single reaction recognize their cognate allele-specific transcripts, which differ by SNPs or an MNP, and emit fluorescence (VIC or FAM). (C) Mouse X chromosome showing the locations of Lamp2 , Atrx , and the X-inactivation center (Xic). (D) Linear correlation between the ratio of the allele-specific cDNA amounts and the ratio of emitted fluorescence for each allele. cDNAs derived from homozygous B6 and Sp mice were mixed at five different ratios, and qRT-PCR was performed using TaqMan probes for the Lamp2 or Atrx gene. The square of the correlation coefficient is indicated by R 2 . (E) Relative abundance of allele-specific transcripts in drug-selected MEFs. The relative abundances of allele-specific transcripts in HAT- or 6-TG-selected bsMEFs were calculated by using TaqMan probes for Lamp2 or Atrx . Data represent mean ± SEM of at least three independent experiments. ∗ p < 0.05 and ∗∗ p < 0.01. n.s., not significant.

Article Snippet: Dissociated cells were subjected to single-cell sorting using MoFlo XDP (Beckman) followed by cDNA synthesis using the RT-RamDA cDNA Synthesis Kit (Toyobo).

Techniques: Isolation, Fluorescence, Derivative Assay, Quantitative RT-PCR