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Image Search Results
Journal: Stem Cell Reports
Article Title: Early reactivation of clustered genes on the inactive X chromosome during somatic cell reprogramming
doi: 10.1016/j.stemcr.2021.11.008
Figure Lengend Snippet: Establishment of a TaqMan-based system to quantify allele-specific transcripts from the X chromosomes (A) Isolation of hybrid MEFs for XCR analyses. (B) TaqMan probes that discriminate transcripts from B6 and Sp alleles. TaqMan probes in a single reaction recognize their cognate allele-specific transcripts, which differ by SNPs or an MNP, and emit fluorescence (VIC or FAM). (C) Mouse X chromosome showing the locations of Lamp2 , Atrx , and the X-inactivation center (Xic). (D) Linear correlation between the ratio of the allele-specific cDNA amounts and the ratio of emitted fluorescence for each allele. cDNAs derived from homozygous B6 and Sp mice were mixed at five different ratios, and qRT-PCR was performed using TaqMan probes for the Lamp2 or Atrx gene. The square of the correlation coefficient is indicated by R 2 . (E) Relative abundance of allele-specific transcripts in drug-selected MEFs. The relative abundances of allele-specific transcripts in HAT- or 6-TG-selected bsMEFs were calculated by using TaqMan probes for Lamp2 or Atrx . Data represent mean ± SEM of at least three independent experiments. ∗ p < 0.05 and ∗∗ p < 0.01. n.s., not significant.
Article Snippet: Dissociated cells were subjected to single-cell sorting using MoFlo XDP (Beckman) followed by cDNA synthesis using the
Techniques: Isolation, Fluorescence, Derivative Assay, Quantitative RT-PCR