Journal: Tissue Engineering and Regenerative Medicine

Article Title: In Vivo Safety and Regeneration of Long-Term Transported Amniotic Fluid Stem Cells for Renal Regeneration

doi: 10.1007/s13770-018-0162-6

Figure Lengend Snippet: Antibodies used in immunohistochemistry for human cell tracking and inflammation analysis

Article Snippet: The details of antibodies used for IHC are provided in Table . table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Antibody Company Dilution Primary antibody CD4 BD Pharmingen 1:200 CD8 Biorbyt 1:200 Human nuclei (HuNu) Millipore 1:200 Secondary antibody Alexa Fluor 594 Goat anti-rabbit IgG Thermo 1:1000 Alexa Fluor 594 Goat anti-mouse IgG Thermo 1:1000 Open in a separate window Antibodies used in immunohistochemistry for human cell tracking and inflammation analysis For real-time PCR, total RNA was isolated from each group using Trizol reagent (Thermo Fisher Scientific, Waltham, MA, USA) and cDNA was synthesized from 1 μg of RNA using a reverse transcription kit (Applied Biosystems, Foster City, CA, USA).

Techniques: Immunohistochemistry, Cell Tracking Assay

Journal: Tissue Engineering and Regenerative Medicine

Article Title: In Vivo Safety and Regeneration of Long-Term Transported Amniotic Fluid Stem Cells for Renal Regeneration

doi: 10.1007/s13770-018-0162-6

Figure Lengend Snippet: Immunohistochemical analysis of kidneys implanted with freshly harvested cells or transported cells. A Cytotoxic T cells identified using an anti-CD4 antibody. B Helper T cells identified using an anti-CD8 antibody. C Human amniotic fluid stem cells (AFSCs) tracked using the human nuclear-specific (HuNu) antibody. D Human AFSC migration into distant organs tracked using the HuNu antibody. E Identification of human β–globin to quantitatively demonstrate that the introduced human cells did not migrate to other distant organs. Arrow: Cells positive for each antibody. Control, sham operated normal kidney; PLGA, kidney implanted with a poly(lactide-co-glycolide) (PLGA) scaffold (without cells); Fresh cell, kidney implanted with freshly harvested cells seeded on a PLGA scaffold; Transport cell, a kidney implanted with transported cells (under optimized conditions) seeded on a PLGA scaffold. n = 5 for each group scale bar 20 μm

Article Snippet: The details of antibodies used for IHC are provided in Table . table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Antibody Company Dilution Primary antibody CD4 BD Pharmingen 1:200 CD8 Biorbyt 1:200 Human nuclei (HuNu) Millipore 1:200 Secondary antibody Alexa Fluor 594 Goat anti-rabbit IgG Thermo 1:1000 Alexa Fluor 594 Goat anti-mouse IgG Thermo 1:1000 Open in a separate window Antibodies used in immunohistochemistry for human cell tracking and inflammation analysis For real-time PCR, total RNA was isolated from each group using Trizol reagent (Thermo Fisher Scientific, Waltham, MA, USA) and cDNA was synthesized from 1 μg of RNA using a reverse transcription kit (Applied Biosystems, Foster City, CA, USA).

Techniques: Immunohistochemical staining, Migration, Control