rnasin Promega Search Results


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  • 90
    Thermo Fisher 11836170001 superasin thermo am2696 rnasin promega n2515
    11836170001 Superasin Thermo Am2696 Rnasin Promega N2515, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 16 article reviews
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    84
    Promega rnasin promega
    RT–PCR amplification of nifH mRNA from wood-fed P. nigrolineatus GI tract regions. After dissection, RNA was extracted from fore (lane 2), hind (lane 3) and midgut (lane 4) regions, as described in the materials and methods section. RNA (3 μg) was first treated with <t>DNaseI</t> in the presence of <t>RNasin</t> and RT–PCR was carried out using the Superscript III One-Step Platinum Taq high fidelity kit (see Methods). Complementary DNA synthesis was followed by amplification with nifH32 and nifH623 primers and a second round amplification using nifH1 and nifH2 primers as described in the materials and methods section. Reaction products were separated on a 1.5% agarose gel. RNA preparations treated with DNase in the absence of RT and amplifications without DNase and RT treatments are shown. M is a 100 bp ladder; lane 1 is a negative control that did not contain RNA.
    Rnasin Promega, supplied by Promega, used in various techniques. Bioz Stars score: 84/100, based on 1272 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Promega rnasin plus rnase inhibitor
    FUS forms a complex with itself, PABP and RNA. ( A ) Co-immunoprecipitation of HA-FUS, Myc-FUS and PABP from HEK293T cells. Immunoprecipitation with a Myc (mouse anti-Myc, CST) antibody pulled down PABP (mouse anti-PABP, Sigma) along with anti-HA-FUS (rabbit HA, CST). <t>RNasin</t> was added to block all <t>RNase</t> activity. ( B ) The interaction between HA-FUS and Myc-FUS was not altered by the addition of RNase, while the co-immunoprecipitation of PABP was completely abolished. ( C ) Immunoprecipitation of FUS (mouse anti-FUS, Santa Cruz) from untransfected cells shows that endogenous FUS (rabbit anti-FUS, Novus Biologicals) also interacts with PABP (mouse anti-PABP, Sigma) and treatment with RNase shows that this interaction is also dependent on RNA. ( D ) Mock immunoprecipitation experiments with either untransfected cells or a single transfection of either HA-FUS WT or Myc-FUS WT with no antibody showed that neither endogenous nor tagged FUS binds to the beads. ( E ) Immunoprecipitation of single transfections with an antibody to the wrong tag showed that a Myc antibody does not pull down HA-FUS and a HA antibody does not precipitate Myc-FUS. FT indicates flow-through of proteins that did not bind to the beads.
    Rnasin Plus Rnase Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1553 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega recombinant rnasin rnase inhibitor
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    Recombinant Rnasin Rnase Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 328 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega rnasin lcb promega
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    Rnasin Lcb Promega, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega rnasin inhibitor
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    Rnasin Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 268 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    Promega ll rnasin
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    Ll Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega placental rnasin
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    Placental Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Promega super rnasein
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    Super Rnasein, supplied by Promega, used in various techniques. Bioz Stars score: 83/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Promega rnaase rnasin
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    Rnaase Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega u rnasin
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    U Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Promega rnasin 1
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    Rnasin 1, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Promega ui rnasin
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    Ui Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Promega iu rnasin
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    Iu Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Promega 1ul rnasin
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    1ul Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Promega rnasin enzyme
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    Rnasin Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Promega isoamyl alcohol rnasin plus rnase inhibitor
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    Isoamyl Alcohol Rnasin Plus Rnase Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Promega 20u milliliter rnasin
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    20u Milliliter Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega u reaction rnasin
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    U Reaction Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega protector rnasin inhibitor
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    Protector Rnasin Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Promega rnasin ribonucleotide inhibitor
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    Rnasin Ribonucleotide Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    Promega rnasin ribounclease inhibitor
    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
    Rnasin Ribounclease Inhibitor, supplied by Promega, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
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    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
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    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
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    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
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    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
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    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
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    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
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    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
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    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
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    2′-F modified <t>siRNA</t> inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, <t>RNasin,</t> ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.
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    Image Search Results


    RT–PCR amplification of nifH mRNA from wood-fed P. nigrolineatus GI tract regions. After dissection, RNA was extracted from fore (lane 2), hind (lane 3) and midgut (lane 4) regions, as described in the materials and methods section. RNA (3 μg) was first treated with DNaseI in the presence of RNasin and RT–PCR was carried out using the Superscript III One-Step Platinum Taq high fidelity kit (see Methods). Complementary DNA synthesis was followed by amplification with nifH32 and nifH623 primers and a second round amplification using nifH1 and nifH2 primers as described in the materials and methods section. Reaction products were separated on a 1.5% agarose gel. RNA preparations treated with DNase in the absence of RT and amplifications without DNase and RT treatments are shown. M is a 100 bp ladder; lane 1 is a negative control that did not contain RNA.

    Journal: The ISME Journal

    Article Title: Nitrogenase diversity and activity in the gastrointestinal tract of the wood-eating catfish Panaque nigrolineatus

    doi: 10.1038/ismej.2015.65

    Figure Lengend Snippet: RT–PCR amplification of nifH mRNA from wood-fed P. nigrolineatus GI tract regions. After dissection, RNA was extracted from fore (lane 2), hind (lane 3) and midgut (lane 4) regions, as described in the materials and methods section. RNA (3 μg) was first treated with DNaseI in the presence of RNasin and RT–PCR was carried out using the Superscript III One-Step Platinum Taq high fidelity kit (see Methods). Complementary DNA synthesis was followed by amplification with nifH32 and nifH623 primers and a second round amplification using nifH1 and nifH2 primers as described in the materials and methods section. Reaction products were separated on a 1.5% agarose gel. RNA preparations treated with DNase in the absence of RT and amplifications without DNase and RT treatments are shown. M is a 100 bp ladder; lane 1 is a negative control that did not contain RNA.

    Article Snippet: RNA (3 μg) was first treated with DNaseI (Fermentas, Hanover, MD, USA) in the presence of RNasin (Promega, Madison, WI, USA) and RT–PCR was carried out using the Superscript III One-Step Platinum Taq high fidelity kit (Invitrogen) with a 15 min complementary DNA synthesis step at 55 °C followed by amplification with nifH32 and nifH623 primers as described above.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Dissection, DNA Synthesis, Agarose Gel Electrophoresis, Negative Control

    FUS forms a complex with itself, PABP and RNA. ( A ) Co-immunoprecipitation of HA-FUS, Myc-FUS and PABP from HEK293T cells. Immunoprecipitation with a Myc (mouse anti-Myc, CST) antibody pulled down PABP (mouse anti-PABP, Sigma) along with anti-HA-FUS (rabbit HA, CST). RNasin was added to block all RNase activity. ( B ) The interaction between HA-FUS and Myc-FUS was not altered by the addition of RNase, while the co-immunoprecipitation of PABP was completely abolished. ( C ) Immunoprecipitation of FUS (mouse anti-FUS, Santa Cruz) from untransfected cells shows that endogenous FUS (rabbit anti-FUS, Novus Biologicals) also interacts with PABP (mouse anti-PABP, Sigma) and treatment with RNase shows that this interaction is also dependent on RNA. ( D ) Mock immunoprecipitation experiments with either untransfected cells or a single transfection of either HA-FUS WT or Myc-FUS WT with no antibody showed that neither endogenous nor tagged FUS binds to the beads. ( E ) Immunoprecipitation of single transfections with an antibody to the wrong tag showed that a Myc antibody does not pull down HA-FUS and a HA antibody does not precipitate Myc-FUS. FT indicates flow-through of proteins that did not bind to the beads.

    Journal: Human Molecular Genetics

    Article Title: ALS mutant FUS disrupts nuclear localization and sequesters wild-type FUS within cytoplasmic stress granules

    doi: 10.1093/hmg/ddt117

    Figure Lengend Snippet: FUS forms a complex with itself, PABP and RNA. ( A ) Co-immunoprecipitation of HA-FUS, Myc-FUS and PABP from HEK293T cells. Immunoprecipitation with a Myc (mouse anti-Myc, CST) antibody pulled down PABP (mouse anti-PABP, Sigma) along with anti-HA-FUS (rabbit HA, CST). RNasin was added to block all RNase activity. ( B ) The interaction between HA-FUS and Myc-FUS was not altered by the addition of RNase, while the co-immunoprecipitation of PABP was completely abolished. ( C ) Immunoprecipitation of FUS (mouse anti-FUS, Santa Cruz) from untransfected cells shows that endogenous FUS (rabbit anti-FUS, Novus Biologicals) also interacts with PABP (mouse anti-PABP, Sigma) and treatment with RNase shows that this interaction is also dependent on RNA. ( D ) Mock immunoprecipitation experiments with either untransfected cells or a single transfection of either HA-FUS WT or Myc-FUS WT with no antibody showed that neither endogenous nor tagged FUS binds to the beads. ( E ) Immunoprecipitation of single transfections with an antibody to the wrong tag showed that a Myc antibody does not pull down HA-FUS and a HA antibody does not precipitate Myc-FUS. FT indicates flow-through of proteins that did not bind to the beads.

    Article Snippet: 5 µl of an RNase A/T1 mix (Fermentas, Yorkshire, UK) or RNasin Plus RNase inhibitor (Promega, Southampton, UK) was added to the tubes and incubated at 37°C for 5 min, then placed back on ice.

    Techniques: Immunoprecipitation, Blocking Assay, Activity Assay, Transfection, Flow Cytometry

    2′-F modified siRNA inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, RNasin, ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.

    Journal: RNA

    Article Title: In vivo activity of nuclease-resistant siRNAs

    doi: 10.1261/rna.5239604

    Figure Lengend Snippet: 2′-F modified siRNA inhibits gene expression in living mice. Representative bioluminescence images of light emitted from living mice transfected, 2 d earlier, with the luciferase expression plasmids pGL3-control. Mice ( N = 4–5 mice per group) received pGL3-control alone ( A ) or were cotransfected with ( B ) 2′-OH GL3 siRNA, ( C ) 2′-OH GL3 siRNA and the RNase inhibitor, RNasin, ( D ) 2′-F GL3 siRNA, ( E ) 2′-F inverted GL2 siRNA. The mouse in panel F received a low pressure i.v. injection of 2′-F GL3 siRNA on day 1 to test if stabilized siRNAs could be taken up by the liver without hydrodynamic transfection. Mice in panels B , C , and D show significant reductions in emitted light as a result of RNA. Hydrodynamic transfection with 2′-F inverted GL2 siRNA and low pressure i.v. transfection of 2′-F GL3 siRNA did not result in significant RNAi.

    Article Snippet: Briefly, 1.8 mL of DPBS (with no MgCl2 or CaCl2 ) was mixed with 3 μg of pGL3-Control (Promega), 3 μg of pThAAT , 10 μg of indicated siRNA, and if indicated, 20 μL of recombinant RNasin (Promega).

    Techniques: Modification, Expressing, Mouse Assay, Transfection, Luciferase, Injection