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Image Search Results
Journal: bioRxiv
Article Title: A Universal, AI-based Design Framework for Efficient Manufacturing of mRNA Therapeutics
doi: 10.64898/2026.03.09.710039
Figure Lengend Snippet: A. A DNA library of 1 million templates that contain 100% of all possible 11-mers is used as input for pooled in vitro transcription (IVT) using different manufacturing processes to create eight IVT-RNA pools. B. Oxford Nanopore Technology Sequencing is used to quantify full-length, (untruncated) DNA and IVT-RNA templates (3 and 8 pools, respectively). The sequencing depths generated are used to calculate a pseudoyield (PY), representative of the amount of full-length (untruncated) IVT-RNA produced per DNA template. C. PY values from the 1M DNA library are used to train, validate and test a Multi-scale Attention Projection Network (MAP-Net), that accurately predicts the PY of unknown DNA templates. MAP-Net is then used within a Genetic Algorithm that generates new synonymously mutated templates with different predicted PY for any DNA template. D. The MAP-Net-based genetic algorithm is used on DNA templates for two real-world mRNA products, hSpCas9 and XBB.8 Spike, generating for each 4 synonymous templates with predicted PYs higher (2) and lower (2) than the starting template. Yields measured for full-length transcripts in individual IVT experiments validate MAP-Net PY predictions. E . The same Genetic Algorithm is now used with MAP-Net and RiboNN as fitness functions to co-optimize the Wuhan Spike sequence for translatability and manufacturability, and results are compared with commercial SARS-COV-2 vaccine sequences.
Article Snippet: To prepare libraries for Illumina sequencing in order to determine
Techniques: In Vitro, Sequencing, Generated, Produced
Journal: bioRxiv
Article Title: A Universal, AI-based Design Framework for Efficient Manufacturing of mRNA Therapeutics
doi: 10.64898/2026.03.09.710039
Figure Lengend Snippet: A : GO term enrichment of the 100 primary transcripts in the human transcriptome with the lowest manufacturability score; shown are the 4 terms with the most significant p-values. B, C : distribution of transcription initiation rates (b) or transcription elongation rates (c) for primary human transcripts with top or bottom 10% predicted PY. D : Predicted PY and TE in the human transcriptome for 19761 primary transcripts from the ENSEMBL database; dotted lines delimit low-medium-high manufacturability (gray) and translatability (orange); numbers in each cell indicate the corresponding number of human transcripts. E : Predicted PY and TE for different codon optimizations of the Spike protein CDS from the SARS-CoV-2 Wuhan-Hu-1strain; wild type sequence (black), codon-optimized sequences using seven commercial software products (grey, see Methods 10.4), BioNTech (orange) and Moderna (blue) commercial vaccine sequences, co-optimized sequences using a genetic algorithm based on MAP-Net and RiboNN (green).
Article Snippet: To prepare libraries for Illumina sequencing in order to determine
Techniques: Sequencing, Software